def calcThermo(seq1, seq2, calc_type='ANY', mv_conc=50, dv_conc=0,
                 dntp_conc=0.8, dna_conc=50, temp_c=37, max_loop=30,
                 temp_only=False):
    """ Main subprocess wrapper for calls to the ntthal executable.

    Returns a named tuple with tm, ds, dh, and dg values or None if no
    structure / complex could be computed.
    """
    args = [pjoin(PRIMER3_HOME, 'ntthal'),
            '-a',       str(calc_type),
            '-mv',      str(mv_conc),
            '-dv',      str(dv_conc),
            '-n',       str(dntp_conc),
            '-d',       str(dna_conc),
            '-t',       str(temp_c),
            '-maxloop', str(max_loop),
            '-path',    THERMO_PATH,
            '-s1',      seq1,
            '-s2',      seq2]
    if temp_only:
        args += ['-r']
    out = subprocess.check_output(args, stderr=DEV_NULL,
                                  env=os.environ)
    return _parse_ntthal(out)
def calcThermo ( seq1 , seq2 , calc_type = 'ANY' , mv_conc = 50 , dv_conc = 0 , dntp_conc = 0.8 , dna_conc = 50 , temp_c = 37 , max_loop = 30 , temp_only = False ) : args = [ pjoin ( PRIMER3_HOME , 'ntthal' ) , '-a' , str ( calc_type ) , '-mv' , str ( mv_conc ) , '-dv' , str ( dv_conc ) , '-n' , str ( dntp_conc ) , '-d' , str ( dna_conc ) , '-t' , str ( temp_c ) , '-maxloop' , str ( max_loop ) , '-path' , THERMO_PATH , '-s1' , seq1 , '-s2' , seq2 ] if temp_only : args += [ '-r' ] out = subprocess . check_output ( args , stderr = DEV_NULL , env = os . environ ) return _parse_ntthal ( out )
Main subprocess wrapper for calls to the ntthal executable.

    Returns a named tuple with tm, ds, dh, and dg values or None if no
    structure / complex could be computed.