Ultrastructural studies of human gingiva. V. Microfibrils of elastic nature and their direct penetration of the basal lamina in chronic periodontitis. In this study, microfibrils widely distributed in the connective tissue of the gingival wall of periodontal pockets were examined by electron microscopy, Theirdistribution, ultrastructural characteristics, close association with fibroblasts and direct communication with the basal lamina were shown. The microfibrils in our specimens were observed in three different groupings (Types A, B, and C). They were believedto represent different stages of maturation of elastic fibera. There was a striking ultrastructural resemblance between oxytalan microfibrils, and the immature or imcompletetype of elastic fiber shown in this report.


Myofascial pain dysfunction syndrome involving tumor metastasis. Case report. This report is an example of tumor metastasis to the temporomandibular joint and its effect on pain, restriction of jaw movement, and the subsequent inability of the patient to function normally. Neoplasm is not the usual etiology of myofascial pain dysfunctionsyndrome and the muscle sparm component of this patient's jaw dysfunction was emphasizedby the relief of symptoms from the use of symptomatic reversible type therapy (tranquilizer, muscle relaxant, and heat). There was an overlay of stress-tension factors in this case history that contributed to the muscle spasm symptoms of the patient. There canbe other etiological factors in jaw dysfunction associated with TMJ pathology such asdevelopmental anomalies, trauma, arthritis, and neoplasm, but as a group they comprise only about 5% of TMJ dysfunction problems (D.M. Laskin, personal communication).


Acute nonlymphocytic leukemia. An adult cluster. Between 1970 and 1973, seven cases of acute nonlymphocytic leukemia were diagnosed in the village of Elmwood, Wis, and the surrounding area. The patients ranged in age from 14 to 97 years. Interpersonal relationships were noted among all patients in the group. This cluster of cases represents a greater than 20-fold increase in the expected incidence of this disease for this population. Morphologic similarities in the blast cells were seen in all cases. This cluster of cases may bear a relationship to bovine leukemia since the town was located in dairy farming country and because a number of the patients had either worked at or lived near the town creamery.


Effects of central-nervous-system irradiation on neuropsychologic functioning of children with acute lymphocytic leukemia. Two neuropsychologic studies were performed to determine the long-term effects of "prophylactic" cranial or craniospinal irradiation on the psychologic and neurologic functions of children with acute lymphocytic leukemia. In a prospective study, 34 patients with leukemia who received either craniospinal irradiation or cranial irradiation combined with intrathecal methotrexate were evaluated by standardized neurologic and psychologic examinations before and after irradiation. Their performance was compared with that of 27 controls who received irradiation to parts of the body other than the cranium. In a retrospective study, 11 patients with leukemia receiving prophylactic craniospinal irradiation and 12 controls with the disease not receiving such therapy were followed from the second year after either irradiation or the initial hematologic remission. Eighteen months after irradiation in the prospective study and four years after irradiation in the retrospective study, no noteworthy neurologic or psychologic differences were found between subjects and controls.


Post-neonatal infant deaths in the Auckland Hospital Board area 1972. A study has been made of the final, and contributory, causes of post-neonatal deaths in the Auckland Hospital Board area for 1972. There was a high rate of deaths from infection. Maori infants died from pneumonia at a rate 28 times that of european infants. Prevention of these infant deaths will need improvement in parent recognition of illness, medical practitioner availability and training, and hospital facilities.


Industrial hand injuries in Pacific Island immigrants. There is a disturbingly high incidence of heavy machinery, industrial hand mutilation, involving recent Pacific Island immigrants many of whom have a poor comprehension of English. A survey of cases admitted to the Hutt Hospital during eight monts of 1973, showed a proportionately greater incidence of severe injuries amongst these immigrants, and indicates that one causative factor is unsuitable selection of works for this dangerous machinery, coupled with inadequate instruction in its use and safety precautions.


Poisoning and overdosage.

Poisoning and overdosage. Admissions to Waikato Hospital during 1973 for poisoning and drug overdosage are compared with the figures for 1963, with allowances for population growth. The figures are analysed on the basis of age, sex, race and residential area. A real reduction is shown in poisoning of children, but a large increase has occured in adults, especially young adults and particularly in drug overdosage. Some possible factors in this are discussed.


X-ray emmissions from colour television receivers in New Zealand. Colour television receivers produced for the New Zealand market have been tested to ensure that any x-radiation emmissions result in dose rates less than the 0.5mrem/h (500murem/h) limit allowed under the Radiation Protection Regulations, 1973. Five receivers representing the five different models available on the New Zealand market were surveyed and the maximum dose rate measured from any screen was 0.05murem/h. The estimated maximum annual radiation dose to an average viewer from the receivers surveyed will be less than 5murem or less than 5 x 10-minus 3 percent of the dose from natural background radiation. This level of x-radiation is well below that which could constitute a health hazard.


Drug surveillance in general practice: a study of oxprenolol in the treatment of angina. Information on the incidence of adverse drug reactions is essential for rational drug therapy. Accurate information of this type is almost entirely derived from intensive surveillance of hospital patients and may not reflect the situation in general practice. In a sutdy of oxprenolol (Trasicor) in the treatment of angina in general practice, a simple non-comparative assessment of efficacy indicated complete or substantial relief of symptoms in nearly 80 percent of patients. Side effects, which were principally related to beta-blockade and were all reversible, occured in 16.6 percent of patients but necessitated stopping treatment in only 10 percent. Simple studies of this nature may yeild valuable evicence about the benefit-to-risk ratio of drugs used under general practice conditions.


Dieldrin poisoning: case report.

Dieldrin poisoning: case report. A case of dieldrin poisoning is reported. This case exemplifies the factor of weight loss and speculates on possible treatment.


The development of social skills in autistic twins. This case study describes the development of social skills in autistic identical male twins, during a year's attendance, as over-age placements, at their local kindergarten. Management techniques were based on behavioural principles; and a team approach was employed.


The later growth of children who were preterm and small for gestational age. The four-year-old growth status of 56 preterm, 35 small for gestational age, and 111 randomly selected appropriate weight for gestational age children who were born at the Queen Mary Hospital (Dunedin) in 1968, is described. Children who were preterm but were greater than 2500g at birth were significantly heavier, taller and had larger head circumference measures than the control group. Preterm children who had weighed less than 2500g at birth were significantly lighter than the controls. Children who were smaller for gestational age were significantly shorter and lighter than the control group. Other results were reported on relationships between gestational age and birth weight and the later growth status of children, and some implications were discussed.


Characteristics of New Zealand women seeking abortion in Melbourne, Australia. In a 24-month period 145 New Zealand women sought abortion in a Melbourne practice, comprising 10 percent of such patients seen. Some 4000 New Zealanders are estimated to fly into Australia annually because of less restrictive abortion laws in some Australian states. The age range in this sample of 145 was from 14-43 years with the mean average age at 21.4 years. Only 24 (16.5 percent) had ever been married and 111 (76.5 percent) were pregnant for the first time. Almost half, 63 (43.5 percent), had never used any contraception in the past. Of the 142 patients aborted, 71 (50 percent) were at least 11 weeks pregnant, four being 14 weeks. Obstacles along the route to abortion, personal, medical and those due to international airline schedules contributed to delaying the date of the operation. The operative procedure was evacuation curettage performed in a private hospital under general anaesthesia. Patients received contraceptive education and returned to their local New Zealand doctor for follow-up.


Acute myocardial infarction in Suva, Fiji. A review of 100 cases of acute myocardial infarction admitted to the Colonial War Memorial Hospital in 1964-65 is presented. The incidence of acute myocardial infarction in this series was 97 percent in Indians and 3 percent in Fijians. The predisposing factors such as diabetes mellitus, hypertension and hypercholesterolaemia are commoner in Indians than Fijians and their role in these patients are described. The mortality rate in the first week was 23.7 percent. A two bed coronary care unit was opened in this hospital last year as part of the overall care for these patients.


Torulopsis glabrata endocarditis complicating aortic homograft valve treated with 5-fluorocytosine: case report with discussion of antifungal chemotherapy. A case of Torulopsis glabrata endocarditis occurring in a patient 14 months after aortic homograft valve replacement is reported. The infection was not controlled by amphotericin B which led to progressive renal impairment. Re-operation was delayed by the development of multiple infarctions due to coronary emboli. The infection was subsequently eradicated by oral treatment with the newer antifungal agent, 5-fluorocytosine, but death of the patient eventually occurred from an arrhythmia related to the persisting myocardial failure consequent upon episodes of transmural infarction. Current evidence favours the use of early re-operation in all cases of endocarditis in addition to aggressive chemotherapy with a combined regime of amphotericin B and 5-fluorocytosine. Clinical pharmacology of 5-fluorocytosine is briefly discussed.


A simplified exercise for the temporomandibular joint following codylotomy. A technique of fabricating an exerciser to prevent ankylosis or reankylosis ofthe condyles is presented which is relatively simple and inexpensive to make. The technique and a brief case report are illustrated and discussed.


The oral component of Sjögren's syndrome. This study reports the results of an interdisciplinary approach to the diagnosis of Sjögren's syndrome in 100 patients. Ocular and systemic diagnoses and oral features from the history and physical examination are correlated with measurements of stimulated parotid flow rate and labial salivary gland histopathology. A new diagnostic criterion is introduced whereby labial salivary gland focus scores are used to establish the presence of the oral component of Sjögren's syndrome in place of the subjective evaluation of xerostomia. The differential diagnosis of the oral clinical features of Sjögren's syndrome and the clinical management of the oral component of this disease are discussed.


Odontogenic myxoma. A clinical and ultrastructural study. The odontogenic myxoma is an uncommon neoplasm of the jaws that is believed to arise from the primitive, mesenchymal portion of the tooth germ. This study surveys nine cases of odontogenic myxoma of the jaws. Analysis of the clinical material revealed that the myxoma occurred most frequently in the posterior regions of the mandible and will recur if initial therapy is too conservative. The average age of the patients in this series was 26.5 years. Five cases involved females and four involved males. Electron microscope findings revealed that the tumor cells are probably responsible for the secretion of the myxomatous intercellular material of the neoplasm.


Unusual oral lesions in a uremic patient. Review of the literature and report of a case. Uremia is defined as the accumulation of nitrogenous waste products in the blood. Uremia may be caused by either acute or chronic renal failure. Uremic stomatitis represents a relatively uncommon intraoral complication of uremia. Uremic stomatitis has classically been divided into ulcerative and nonulcerative types. Reported here is a patient with chronic renal failure exhibiting intraoral lesions that persisted despite local treatment but rapidly cleared following renal dialysis. This case represents the first published report of the microscopic appearance of the nonulcerative type and presents unusual tissue changes heretofore unreported.


Double bilateral dens invaginatus in the maxillary incisor region. Two cases of bilateral double dens invaginatus in the maxillary incisor region have been reported. One of the patients (Case 1), a 12-year-old boy, had double invaginations in both of the erupted teeth in the maxillary lateral incisor region. The other patient (Case 2), a boy 8 years of age, had double invaginations in each of the two partially developed supernumerary teeth that were impacted in the palate in the maxillary central incisor region. Double bilateral dens invaginatus would appear to be a most unusual occurrence.


Synchronous malignant mucoepidermoid tumor of the parotid gland and Warthin's tumor in adjacent lymph node. A case of a malignant mucoepidermoid tumor (poorly differentiated) occurring simultaneously with a homolateral Warthin's tumor is presented. The simultaneous occurrence of two salivary gland tumors of different types is extremely rare. Only five cases have been reported in the literature. The occurrence of a malignant mucoepidermoid tumor and a Warthin's tumor in the same patient has not been previously reported.


Radiologic features of ameloblastoma in Nigerians. Radiology plays an important role in preoperative diagnosis and postoperative assessment of ameloblastoma. The postoperative appearances, including complications as well as radiologic features of recurrence, are discussed. The differential diagnosis of the unilocular lesion is difficult, and particularly noteworthy are the five cases of histologically proven adamantinoma whose radiographic appearance closely mimicked dentigerous cyst.


Lerbek, a synergistic mixture of methyl benzoquate and clopidol for the prevention of chicken coccidiosis. Methyl benzoquate at 20 ppm or clopidol at 250 ppm in the diet show true coccidiostatic activity in infections with Eimeria tenella in the chick. Lerbek, a synergistic mixture yielding 8-35 ppm methyl benzoquate and 100 ppm clopidol, produced a partially coccidiocidal effect; relapses following restricted periods of medication were less severe, and suppression of parasite development following delayed medication was quicker and more effective with either component of the mixture used alone.


Prenatal diagnosis of type II glycogenosis (Pompe's disease) using microchemical analyses. 1. In order to reduce the time interval between amniocentesis and prenatal diagnosis of Pompe's disease microchemical techniques were used for assay of acid alpha-1,4-glucosidase activities in cultured amniotic fluid cells. 2. Microtechniques used on homogenates of cultured amniotic fluid cells enabled the waiting period to be reduced to 2-3 weeks. 3. When dissected lyophilized groups of 200-300 cultured cells were analyzed, a prenatal diagnosis was possible at about 10 days after amniocentesis. 4. The acid alpha-1,4-glucosidase activity in the amniotic fluid supernatant is not informative in prenatal diagnosis of Pompe's disease. 5. Conditions of cell cultivation such as length of time in culture were found to influence markedly the acid alpha-1,4-glucosidase activity in cultured amniotic fluid cells. 6. For a reliable prenatal diagnosis of metabolic disorders primary cultures of control amniotic fluid cells should be used and the analytical results from the pregnancy at risk should be compared with primary cultures of control amniotic fluid cells and with those in cultured fibroblasts from heterozygous carriers, and an affected sibling from the particular family.


[Dental damage after radiotherapy for malignant lesions in the head and neck region]. A review is presented of dental damage resulting from radiotherapy for malignant lesions in the head and neck region.


[Some indices of the fibrinolytic system of the blood in patients with acute leukemia]. An attempt is made to ascertain the mechanism of increased fibrinolytic activity in patients suffering from acute leukemia. Investigations confirmed a possibility of both the primary and the secondary character of hyperfibrinolysis which required the choice of rational therapeutic tactics in each individual case.


Ozone model for bonding of an O2 to heme in oxyhemoglobin. Several rather different models of the Fe-o2 bond in oxyhemoglobin have previously been proposed, none of which provide a satisfactory explanation of several properties. We propose a new model for the bonding of an O2 to the Fe of myoglobin and hemoglobin and report ab initio generalized valence bond and configuration interaction calculations on FeO2 that corroborate this model. Our model is based closely upon the bonding in ozone which recent theoretical studies have shown to be basically a biradical with a singlet state stabilized by a three-center four-electron pi bond. In this model, the facile formation and dissociation of the Fe-O2 bond is easily rationalized since the O2 always retains its triplet ground state character. The ozone model leads naturally to a large negative electric field gradient (in agreement with Mössbauer studies) and to z-polarized (perpendicular to the heme) charge transfer transitions. It also suggests that the 1.3 eV transition, present in HbO2 and absent in HbCO, is due to a porphyrin-to-Fe transition, analogous to that of ferric hemoglobins (e.g., HbCN).


Third component of complement (C3): structural properties in relation to functions. The third component of complement (C3) fulfills a pivotal role in the functions of the complement system. We have investigated the topological relationships among its polypeptide chains, physiologic fragments, enzyme attack regions, and functional sites. C3 consists of two chains (alpha and beta) which are linked by disulfide bonds and noncovalent forces and which have molecular weights of, respectively, 120,000 and 75,000. C3 is activated by action of C3 convertase on the alpha-chain. With hydrolysis of one polypeptide bonds, C3a, the 9000 dalton activation peptide is dislocated from the NH2-terminal portion of the alpha-chain. A previously concealed binding region is thereby transiently revealed in the C3b-fragment (181,000 dalton) which displays affinity for apparently nonspecific acceptors present on biological membranes. Binding of nascent C3b membranes occurs through the C3d portion of the fragment because subsequent action of the C3b-inactivator or trypsin on bound C3b causes release of C3c, but not of C3d. Bound C3b and C3d possess stable sites that are capable of binding to specific receptors present on a limited variety of cells. We propose that all known physiologically occurring fragments of C3 arise by enzymatic cleavage of the alpha-chain: C3a, C3b, C3c, and C3d. Whereas C3a (alpha1) and C3e (alpha2) consist of a single chain and C3b consists of two chains (alpha' and beta), C3c is composed of the entire beta-chain and multiple fragments of the alpha-chain, each of which is linked by disulfide bonds to the beta-chain.


Reactions of migrating birds to lights and aircraft. Midair collsions between birds and aircraft pose a hazard for both. While observing migrating birds with a tracking radar, we find that birds often react, by taking evasive maneuvers, at distances of 200-300 m to both searchlight beams and the approach of a small airplane with its landing lights on. Appropriately arranged lights on aircraft should decrease the hazard of collisions with birds.


Sidechain torsional potentials and motion of amino acids in porteins: bovine pancreatic trypsin inhibitor. Conformational potentials of sidechains in the bovine pancreatic trypsin inhibitor have been studied with an empirical energy function. Calculated minimumenergy positions are in excellent agreement with the x-ray structure for sidechains in the core or at the surface of the protein; as expected, angles for sidechains that are directed out into the solvent do not agree with the calculated values. The contributions to the potentials are analyzed and compared with the potentials for the free amino acid. Although there is a large restriction in the available conformational space due to nonbonded interactions, the minimum energy positions in the protein are close to those of the free amino acid; the significance of this result is discussed. To estimate the effective barriers for rotation of the aromatic rings (tyrosine and phenylalanine), calculations are done in which the protein is permitted to relax as a function of the ring orientation. Thr resulting barriers, which are much lowere than the rigid rotation barriers, are used to evaluate the rotation rates; comparison is made with the available nuclear magnetic resonance data.


Method for predicting RNA secondary structure. We report a method for predicting the most stable secondary structure of RNA from its primary sequence of nucleotides. The technique consists of a series of three computer programs interfaced to take the nucleotide sequence of any RNA and (a) list all possible helical regions, using modified Watson-Crick base-pairing rules; (b) create all possible secondary structures by forming permutations of compatible helical regions; and (c)evaluate each structure for total free energy of formation from a completely extended chain. A free energy distribution and the base-by-base bonding interactions of each possible structure are catalogued by the system and are readily available for examination. The method has been applied to 62 tRNA sequences. The total free-energy of the predicted most stable structures ranged from -19 to -41 kcal/mole (-22 to -49 kJ/mole). The number of structures created was also highly sequence-dependent and ranged from 200 to 13,000. In nearly all cases the cloverleaf is predicted to be the structure with the lowest free energy of formation.


Failure of the opiate antagonist naloxone to modify hypnotic analgesia. Hypnotic analgesia in some respects resembles opiate analgesia. We tested the hypothesis that some features of hypnotic analgesia are mediated through neuronal pathways activating specific opiate receptors in brain. The opiate antagonist naloxone had no effect on hypnotic analgesia in three subjects. Thus, the hypothesis was not confirmed.


Physical basis of charge pairing in mitochondria. The postulate of charge pairing in the mitochondrial inner membrane is justified by applying a formula due to Fuoss to calculate the probability density for the distance between a positive and a negative charge. For dielectric constants 10 or less pairing is absolute, for 20 there is some tendency towards pairing, and at 78 it is nonexistent. Pairing, partner exchange or charge substitution, inhibition, and antiport uncoupling can be rationalized within this framework.


Mechanism and modification of bradykinin-induced coronary vasodilation. In isolated perfused rabbit hearts, bradykinin produced a concentration-dependent decrease in coronary resistance directly associated with biosynthesis and release of prostaglandin-E-like substance. An inhibitor of bradykinin destruction (the nonapeptide SQ-20881) markedly enhanced both the coronary vasodilation and release of prostaglandin-E-like substance produced by cardiac injection of bradykinin. Indomethacin inhibited both the myocardial prostaglandin biosynthesis and the decrease in coronary resistance induced by bradykinin. The demonstration that bradykinin is a potent stimulator of prostaglandin biosynthesis in the heart has implications as to the cause of the afferent cardiovascular reflexes and pain in myocardial infarction and angina pectoris.


Somatic rearrangement of chromosome 14 in human lymphocytes. Ataxia-telangiectasia is a rare genetic disorder associated with immune deficiency, chromosome instability, and a predisposition to lymphoid malignancy. We have detected chromosomally anomalous clones of lymphocytes in eight patients with this disorder. Chromosome banding disclosed that the clones are consistently marked by structural rearrangement of the long arm (q) of chromosome 14. A translocation involving 14q was found in clones obtained from seven of the eight patients whereas a ring 14 chromosome was found in a clone obtained from the other. These findings as well as data obtained by others for patients with ataxia-telangiectasia suggest that structural rearrangement of 14q is the initial chromosomal change in lymphocyte clones of patients with this disorder. Chromosomes of lymphocytes from one of the patients were studied before and after the onset of chronic lymphocytic leukemia. Before leukemia was diagnosed, the patient had a lymphocyte clone with a 14q translocation. This clone appears to have given rise to the leukemic cells. We hypothesize that structural rearrangement of 14q is directly related to abnormal growth of lymphocytes and that it may be a step toward the development of lymphoid malignancies. Increasing evidence, provided by others, for the nonrandom involvement of 14q in African-type Burkitt's lymphoma and other lymphoid neoplasms further strengthens this hypothesis.


Localization of the IgG effector site for monocyte receptors. A peptide consisting of 10 amino acids derived from the CH3 region of human IgG was shown to bind to monocytes and to inhibit rosette formation of antibody-coated erythrocytes with human monocytes. Two myeloma proteins of the IgG1 and IgG3 subclass, both with known deletions in the CH2 region of the gamma chain, showed unimpaired ability to bind to monocytes. These experiments suggest that the isolated peptide represents the primary site of attachment of IgG to monocytes.


Atomic structure of intracellular amorphous calcium phosphate deposits. The radial distribution function calculated from x-ray diffraction of mineralized cytoplasmic structures isolated from the hepatopancreas of the blue crab (Callinectes sapidus) is very similar to that previously found for synthetic amorphous calcium phosphate. Both types of mineral apparently have only short-range atomic order, represented as a neutral ion cluster of about 10 A in longest dimension, whose probable composition is expressed by the formula Ca9(PO4)6. The minor differences observed are attributed to the presence in the biological mineral of significant amounts of Mg-2+ and ATP. Synthetic amorphous calcium phosphate in contact with a solution containing an amount of ATP equivalent to that of the biological mineral failed to undergo conversion to the thermodynamically more stable hydroxyapatite. The amorphous calcium phosphate of the cytoplasmic mineral granules is similarly stable, and does not undergo conversion to hydroxyapatite, presumably owing to the presence of ATP and Mg-2+, known in inhibitors of the conversion process. The physiological implications of mineral deposits consisting of stabilized calcium phosphate ion clusters are discussed.


Lymphokine-induced production and release of lysosomal enzymes by macrophages. MACROPHAGES ARE ASSOCIATED WITH MOST CHRONIC INFLAMMATORY LESIONS, AND THESE CELLS CONTAIN ENZYMES THAT ARE ABLE TO DESTROY CONNECTIVE TISSUE CONSTITUENTS. Normal lymphoid cells responding to a mitogen, phytohemagglutinin-P, release factor(s) that cause a marked increase in the size and enzyme content for mononuclear phagocytes maintained in culture. The stimulated macrophages, which by several criteria remain otherwise viable and healthy, selectively release large quantities of hydrolytic enzymes to the culture medium.


Detection of new temperature-dependent conformational transition in lysozyme by carbon-13 nuclear magnetic resonance spectroscopy. A specific temperature-dependent conformational transition of hen egg-white lysozyme, occurring between 20 degree C and 30 degree C in solution, has been detected by 13-C-nuclear magnetic resonance spectroscopy. Selective changes in the chemical shifts of aromatic residues, together with differences in the chemical shifts, and nuclear Overhauser enhancement in the carbonyl, carboxyl, and alpha-carbon regions of the spectrum point to the vicinity of subsites D and E as the primary locus of the structural change.


Human X-Linked genes regionally mapped utilizing X-autosome translocations and somatic cell hybrids. Human genes coding for hypoxanthine phosphoribosyltransferase (HPRT, EC 2.4.2.8; IMP:pyrophosphate phosphoribosyltransferase), glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49; D-glucose-6-phosphate:NADP+ 1-oxidoreductase), and phosphoglycerate kinase (PGK, EC 2.7.2.3; ATP:3-phospho-D-glycerate 1-phosphotransferase) have been assigned to specific regions on the long arm of the X chromosome by somatic cell gentic techniques. Gene assignment and linear order were determined by employing human somatic cells possessing an X/9 translocation or an X/22 translocation in man-mouse cell hybridization studies. The X/9 translocation involved the majority of the X long arm translocated to chromosome 9 and the X/22 translocation involved the distal half of the X long arm translocated to 22. In each case these rearrangements appeared to be reciprocal. Concordant segregation of X-linked enzymes and segments of the X chromosome generated by the translocations indicated assignment of the PGK gene to a proximal long arm region (q12-q22) and the HPRT and G6PD genes to the distal half (q22-qter) of the X long arm. Further evidence suggests a gene order on the X long arm of centromere-PGK-HPRT-G6PD.


Genetic variation as a test of natural selection. Allozymic variation encoded by 26 loci was analyzed electrophoretically in 507 specimens representing 12 populations of green toads, Bufo viridis, in Israel and the Vis Adriatic  Island. Genetic variation in Bufo viridis is higher than in any vertebrate yet studied. Mean heterozygosity per locus per individual (H) is 0.133 (range, 0.105 to 0.159). H is higher in central populations as compared with isolates, and varies among four major protein classes, being highest in transferases and hydrolases and lowest in oxidoreductases and nonenzymatic proteins. Differential gene frequencies among polymorphisms was tested as an indicator of natural selection. Significant heterogeneity between loci in their apparent inbreeding coefficients Fe=S-2p/P(1-p) was found for all alleles and for each of the four major classes of proteins tested, which may be taken as evidence of selection. Both uniform and diversifying selection are suggested by the low and high Fe values, respectively. The general pattern of high heterozygosity in Bufo viridis is best explained as an adaptive strategy in heterogeneous environments.


Tertiary structure of myohemerythrin at low resolution. X-ray diffraction studies have produced a low resolution image and also located the iron atoms of a monomeric hemerythrin from muscles of a sipunculan worm. These results reveal the course of the polypeptide chain and some details of the active center.


Mouse SmI cortex: qualitative and quantitative classification of golgi-impregnated barrel neurons. The neurons in layer IV of the mouse somatosensory cortex are arranged in a remarkably consistent pattern of multicellular cytoarchitectonic units called "barrels." Each barrel is known to be related, in a one to one manner, to a contralateral whisker or vibrissa on the animal's face. In this study we have examined Golgi-impregnated neurons that comprise the "barrels." Several criteria, some being quantitative measures of dendritic arbors and somal sizes which were obtained with a computer-microscope system, suggest that all barrel neurons can be placed in two classes, the members of which are present in approximately equal numbers. The cells in the two classes can be further subdivided on the basis of the relationship of their processes to the barrels: 85% of them have processes restricted to a single barrel; 15% of them distribute their processes to two or more barrels. From these observations it is possible to suggest that a majority of neurons comprising the barrels would respond initially to movements of only one whisker while the remainder would respond to movements of two or more whiskers. In addition it has been shown that the quantitation of neuronal structure can provide a numerical basis for the classification of neurons in the central nervous system.


Alamethicin-mediated fusion of lecithin vesicles. It was recently shown that alamethicin greatly facilitates the fusion of small, sonicated, lecithin bilayer vesicles. In the present work the details of this fusion process have been followed by monitoring the inner and outer choline methyl signals separately by proton magnetic resonance spectroscopy. It is shown that during the alamethicine-induced fusion some of the antibiotic molecules become translocated from the extravesicular aqueous medium into the enclosed intravesicular space, and these alamethicine molecules were found to affect the choline methyl signals from the inner half of the bilayer only. No evidence was obtained for transmembrane coupling of the two halves of the bilayer in the presence of alamethicin or for any effects that might be construed as due to incorporation of alamethicin molecules into the hydrophobic core of the bilayer.


Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. Unstacked thylakoid membrane vesicles were obtained from a homogenate of Chlamydomonas reinhardtii by flotation in a 1.8 M sucrose layer containing 5 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid)-10 mM EDTA (pH 7.5). Sodium dodecyl sulfate-gradient gel electrophoresis showed that the wildtype membranes have a total of at least 33 polypeptides ranging in molecular weights from 68,000 to less than 10,000. The wild-type and three non-photosynthetic mutant strains were studied with respect to their photosynthetic electron transport properties, their fluorescence rise kinetics, and their membrane polypeptide compositions. The results showed a strong correlation between the presence of a membrane polypeptide (molecular weight = 47,000) and the activity of the photosystem II reaction center. This polypeptide is missing from F34 (a mendelian mutant lacking Q, the primary electron acceptor of photosystem II), but is partially restored in a suppressed strain of F34 in which there is an incomplete recovery of photosystem II activity. In a thermosensitive mutant, T4, the same polypeptide is present in reduced amount only in cells grown at 35 degrees but not in those grown at 25 degrees. Evidence from fluorescence rise kinetics and partial photochemical reactions show that the cells grown at 25 degree are similar to wild-type cells but the cells grown at 35 degrees are greatly deficient in Q.


Enzymes as reagents in peptide synthesis: enzymatic removal of amine protecting groups. A model system is described for the enzymatic deprotection of suitably masked amino groups during stepwise peptide synthesis. Nitrophenyl esters of amino acids, N-protected with trypsin-labile benzyloxycarbonylarginyl groups, were prepared as crystalline, analytically pure picrate salts in a standardized procedure. These intermediates were shown to react with amino compounds to form the expected peptide linkages. A pair of diasteriomeric peptides prepared in this way and featuring benzyloxycarbonylarginyl-L-, AND -D-glutaminyl sequences, respectively, were subjected to tryptic digestion. In both cases, a specific cleavage of the arginyl bond was achieved; however, the peptide containing the L-glutaminyl residue was deprotected much more rapidly than its diasteriomer containing the D-glutaminyl residue. The hydrolysis of the former isomer was not noticeably impeded by the presence of the latter. The results of these studies suggest that C-activated amino-acid derivatives, N-protected with trypsin-labile groups, are readily prepared in convenient form and that the peptide derivatives prepared from these intermediates are readily freed of their amino-protecting groups under mild, aqueous conditions with a potentially useful degree of stereospecificity. Theoretical implications of this first enzyme-catalyzed step in the repetitive cycle of peptide elaboration are discussed along with the procedural advantages implicit in the alternation of strongly and weakly basic groups in the protected and unprotected peptide intermediates, respectively.


Formation of bone marrow in fibroblast-transformation ossicles. The genesis of hemopoietic bone marrow was studied in matrix-induced transformation plaques and ossicles in subcutaneous spaces of thorax and abdomen of rat. With the advent of blood vessels in the plaque on day 9, there began a rapid and radical conglomerate shift, cartilaginous to osseous, which was nearly total in 72 hr. Incorporation of 59-Fe into heme provided a sensitive quantitative assay for hemopoiesis. On day 12 the first colonies of hemopoietic cells were observed. These developed adjacent to cavernous sinuses which had formed to fill the void left by chondrolysis. Total occupation of the ossicle with hemopoietic marrow was found on days 23-28. The thoracic region was favorable for the formation of hemopoietic marrow, whereas lower abdominal sites were disadvantageous.


Nuclear magnetic resonance spectrum of living tunicate blood cells and the structure of the native vanadium chromogen. The 1-H nuclear magnetic resonance spectrum of living tunicate blood cells was examined in an attempt to develop a biophysical assay for the native vanadium chromogen. The living cell spectrum was found to exhibit a broad 21 ppm downfield Gaussian signal which, however, disappears immediately upon cell disruption. Examination of the properties of this extremely low field signal revealed that it corresponds to a labile vanadium (III) aquo complex contained in the cell vacuoles, that vanadium(III) concentrations are rigidly regulated within these vacuoles, and that artifact formation does occur in the hemolysate. The living cell spectrum also indicates the number of ligand-bound vanadium(III) coordination sites in the native blood pigment. Results are discussed relation to the possible functions of the vandium chromogen.


Natural selection and the evolution of reproductive effort. Reproductive effort is defined as that proportion of the total energy budget of an organism that is devoted to reproductive processes. Reproductive effort at a given age within a species will be selected to maximize reproductive value at that age. Reproductive effort is not directly affected by changes in juvenile survivorship, nor necessarily reduced by an increase in adult survivorship. Selection for high levels of reproductive effort should occur when extrinsic adult mortality is high, in environments with constant juvenile survivorship, and in good years for juvenile survivorship in a variable environment, provided that the quality of the year is predictable by adults. Data necessary to measure reproductive effort and to understand how selection results in different levels of effort between individuals and species are discussed. We make several predictions about the effect of increased resource availability on reproductive effort. The empirical bases for testing these predictions are presently inadequate, and we consider data on energy budgets of organisms in nature to be essential for such test. We also conclude that variance in life table parameters must be known in detail to understand the selective bases of levels of reproductive effort.


Genetic analysis of the cell surface: association of human chromosome 5 with sensitivity to diphtheria toxin in mouse-human somatic cell hybrids. Diphtheria toxin inhibits protein synthesis in eukaryotic cells by catalyzing inactivation of elongation factor 2. The 10,000-fold greater sensitivity in vitro to diphtheria toxin of human cells as compared to mouse cells seems to be attributable to a difference at the level of the cell membrane. Mouse-human cell hybrids are as sensitive to diphtheria toxin as human cells. We have shown that the sensitivity of the hybrid cells is due to a gene or genes located on human chromosome 5. Mouse-human hybrid cells in which chromosome 5 is present are as sensitive to the toxin as human cells, which hybrids without chromosome 5 are as resistant as mouse cells. Entry of toxin into cells seems to be a two-step process involvin, (1) binding of toxin to the cell surface and (2) endocytotic uptake of toxin. The difference in sensitivity between human and mouse cells and between hybrid cells with and without chromosome 5 does not appear to be due to a difference in endocytotic activity and may be due to presence or absence of toxin-specific receptor.


Language boundaries and biological differentiation of Bougainville: multivariate analysis of variance. Blood genetic and antropometric data on Melanesians from Bougainville, Papus New Guinea, are analyzed by random-effects analysis of variance to partition the observed variation into components for the individuals, village, and language group level. Both clinial and unpatterned group differences exist. The differences between language groups appear to be substantial, even when the results are corrected for clinal effects. The amounts of variation of each level correspond roughly to a similar analysis of heterozygosity in blood polymorphisms. Observed current migration figures suggest that language and village constitute a 2-fold hierarchical subdivision of the breeding system, and this analysis shows probable random drift effects between groups at both the village and language level. Both language and genetic constitution of these villages are the result of differentiation in place.


Early effect of interferon on mouse leukemia cells cultivated in a chemostat. Mouse interferon preparations inhibited the multiplication of mouse leukemia L 1210 cells cultivated under steady-state conditions in a chemostat. The use of this sensitive and controlled system led to the detection of a rapid inhibition in the incorporation of (3-H)thymidine into cellular acid-precipitable material 2 hr after the addition of interferon, whereas an effect on cell multiplication was not detected until 22 hr later. Interferon exerted only a transitory effect on the incorporation of (3-H)uridine into acid-precipitable material and no effect on the incorporation of 14-C-amino acids into cellular protein. It is suggested that the chemostat offers many advantages for the investigation of those physiologic factors or chemotherapeutic substances that modify cell division.


Regulation of protein synthesis at the translational level in neuroblastoma cells. Protein synthesis in reuroblastoma cells has been studied in a cell-free system. The activity of lysates from cells grown insuspension and monolayer has been compared. A higher level of activity has been found in monolayer cells. The activity of some components of the lysate that are involved in protein synthesis has been analyzed. The data suggest that the controlling step of protein synthesis in this system might be in the initiation process. The correlation between activation of protein synthesis and neurite outgrowth in monolayer cultures is discussed.


On the prokaryotic nature of red algal chloroplasts. The sequences of oligonucleotides released by T1 ribonuclease digestion of 32-P-labeled 16S (chloroplast) and 18S (cytoplasmic) ribosomal RNAs from a marine species of Porphyridium (Rhodophyta) have been determined. The resultant catalogs have been compared to those obtained for three prokaryotes:Escherichia coli, Bacillus subtilis, and Anacystis nidulans (a blud-green alga). There is extensive sequence homology between the Porphyridium chloroplast 16S ribosomal RNA and each of the prokaryotic 16S ribosomal RNAs, but little homology between the Porphyridium cytoplasmic 18S ribosomal RNA and any of the 16S species. These data provide a measure of the evolutionary distance separating existing chloroplasts from contemporary bacteria and blue-green algae, and are discussed in terms of the hypothesis that these organelles evolved from endosymbiotic photosynthetic prokaryotes.


X-ray absorption spectroscopy using synchrotron radiation for structural investigation of organometallic molecules of biological interest. The technique of x-ray absorption spectroscopy using tuneable, very intense x-rays from a high energy electron storage ring has been applied to study of the estended x-ray absorption fine structure for Cu and Ni tetraphenylporphyrin and methemoglobin. Preliminary analysis shows that the spectra may be interpreted as a super-position of modulations arising from the nearest neighbor nitrogen and pyrrole alpha-carbon coordination sheels of the metal atoms. We estimate that with the observed magnitude of noise to modulation amplitude, relative shifts of 0,5% in the metal-nitrogen to metal-carbon bond distances in the prophyrins should be observable using extended x-ray absorption fine structure and that this technique may provide a method of observing these types of structural changes in solution.


Isolation of rabbit reticulocyte initiation factors by means of heparin bound to sepharose. Passage of cell-free extracts of rabbit reticulocytes through heparin-Sepharose affinity columns results in the loss of the ability of the effluent to initiate protein synthesis. This is shown by the loss of response to added rabbit globin mRNA or to inhibitors of initiation of protein synthesis, such as heparin and aurin tricarboxylic acid, and by recovery of initiation activity by addition of protein retained and subsequently eluted from the columns. The effluent retains, however, the ability to elongate protein chains. Only 0.8% of the applied cell extract protein binds to heparin-Sepharose columns. This bound protein, which can be recovered by increasing the salt concentration of the eluting buffer, has initiation factor activity equal to that of a crude initiation factor preparation obtained from rabbit reticulocyte ribosomes by extraction with 0.5 M KCl. The protein patterns on polyacrylamide gels of the initiation factors prepared by either method are very similar and indicate a protein mixture, which may represent a complex. These data confirm that heparin interacts specifically with initiation factos, and indicate that heparin-Sepharose chromatography will simplify procedures for the preparation of initiation factors.


Effect of chromosomal proteins extractable with low concentrations of NaCl on chromatin structure of resting and proliferating cells. When quiescent WI-38 cells are stimulated to proliferate by nutritional changes, the chromatin of stimulated cells shows an increased ellipticity in circular dichroism spectra and an increased ability to bind ethidium bromide. The differences between the chromatins of quiescent and stimulated cells are abolished if both chromatins are treated with 0.25 M NaCl. However, when salt-treated chromatins are reconstituted by dialysis agains 10 mM Tris-HCl, the difference in circular dichroism spectra and ethidium bromide binding re-appear. Furthermore, addition of the 0.25 M NaCl extract from chromatin of stimulated cells to chromatin of quiescent cells causes an increased ellipticity in circular dichroism spectra similar to that of stimulated chromatin. Since only proteins (mostly nonhistones) are detectable in the 0.25 M NaCl extract, these results strongly suggest that the chromosomal proteins extractable with low concentrations of salt are responsible for the functional and structural changes occurring in chromatin of WI-38 cells stimulated to proliferate.


Kinetics of opiate receptor inactivation by sulfhydryl reagents: evidence for conformational change in presence of sodium ions. The role of SH groups in opiate-receptor interactions has been further examined. In activation by N-ethylmaleimide of sterospecific opiate binding by rat brain membrane fractions follows pseudo-first order kinetics and exhibits strong temperature dependence. The kinetics indicate that alkylation of a single SH group suffices to block opiate binding. Considerable protection from SH group inactivation is observed when treatment with N-ethylmaleimide is carried out in the presence of an opiate or an antagonist, suggesting close proximity of the SH group to the opiate binding site. The rate of inactivation of receptor binding by N-ethylmaleimide is markedly slower in buffers containing 100 mM NaCl (t1/2 equals 30 plus or minus 1.4 min) than in sodium-free buffers (t1/2 equals 10 plus or minus 1.0 min). Since the rate of alkylation of model SH compounds is unaffected by sodium ions, this protection seems best explained by a conformational change in the receptors that renders the SH groups less accessible to alkylation. The rate of inactivation is not affected by K+, Rb+, or Cs+ and only slightly by Li+. This cation specificity as well as the concentration-response to Na+ are remarkably similar to those previously shown to lead to increased antagonist and decreased agonist binding. We suggest that the same conformational change is involved in the two phenomena.


Membrane difference in peripheral blood lymphocytes from patients with chronic lymphocytic leukemia and Hodgkin's disease. Lymphocytes were isolated from the peripheral blood of 21 normal persons and 66 patients with chronic lymphocytic leukemia (CLL), CLL in remission, Hodgkin's disease, Hodgkin's disease in remission, various other tumors, or cardiovascular diseases; The lymphocytes were studied for cap formation and agglutinability by concanavalin A, and for cell attachment to the surface of a petri dish. The frequency of cap formation was lowest in lymphocytes from patients with untreated Hodgkin's disease (2.1 plus or minus 0.8%), next lowest in lymphocytes from patients with CLL who were or were not under treatment (7,0 plus or minus 1;3%), and also low in Hodgkin's disease in remission (10.6 plus or minus 1.2%). The frequencies of cap formation by lymphocytes from patients with various other tumors (19.1 plus or minus 2.5%), with CLL in remission (24.0 plus or minus 0.9%), and with nonmalignant diseases (26.0 plus or minus 2.2%) were more similar to the frequency found in lymphocytes from normal persons (29.4 plus or minus 2.8%). Lymphocytes from all the patients, including those in remission, showed a higher degree of agglutinability by concanavalin A than lymphocytes from normal persons. Cell attachment to a petri dish was highest with CLL, next highest with CLL in remission, and low for normal persons and all the other patients. Lymphocytes from normal persons that consisted predominantly of thymus-derived cells gave similar results to isolated normal bone marrow-derived cells. The results indicate that there were different changes in the surface membrane of lymphocytes from patients with CLL, CLL in remission, Hodgkin's disease, and Hodgkin's disease in remission, and that the patients in clinical remission still showed abnormalities in their lymphocytes.


Cytoplasmic control of nuclear DNA synthesis during early development of Xenopus laevis: a cell-free assay. Nuclei isolated from nondividing cells were induced to synthesize DNA by incubation with cytoplasm from early embryos of Xenopus laevis. Numerous replication eyes were formed in the nuclear DNA molecules, and high levels of [3-H]dTTP were incorporated. With this assay a protein(s) which appears to initiate DNA synthesis was found at high levels in the cytoplasm of eggs, blastulae, or gastrulae, but only at low levels in the cytoplasm of oocytes, hatched embryos, or adult tissues.


Caries reduction 1.5 years after application of a fissure sealant as related to dietary habits. Findings 1.5 years after the application of the sealant revealed that among the 200 sealed permanent first molars, 2.5% of the sealants were lost or partly missing, while 95% of the sealants remained in good condition. During the test period, 7 of the 200 sealed permanent fissures had become carious, while 64 of the respective control fissures had decayed. The percentage effectiveness of the prevention by the present method was 85%. Four out of five molars that had lost the sealants became carious, but of the 190 molars having sealants in good condition, only three were diagnosed as carious. All teeth reported as contaminated with saliva at the sealing session had lost the material. No correlation could be found between the type of food eaten or oral hygiene habits of the subjects and the retention of the sealants.


Contralateral symmetry of acid etched enamel surfaces. Scanning electron microscopy (SEM) of phosphoric acid etched labial enamel surfaces of human permanent incisors and canines showed that the etch pattern, including the depth of the etched relief, may vary significantly from tooth to tooth and between different areas of the same tooth. On the other hand, pairs of contralateral teeth showed a very similar etch pattern symmetrically along the midline. No difference in the pattern could be found in enamel etched with three different orthophosphoric acids: 50% with 7% ZnO, 37%, and 35%. Therefore, when comparing the depth of penetration of different cold-curing restorative resins, composite or non-composite, into the etched enamel relief, it seems appropriate to study these materials two-by-two on pairs of contralateral teeth.


Adaptation of resinous restorative materials to acid etched enamel surfaces. The purpose of this paper was to study by means of scanning electron (SEM) and optical microscopy the adaptation of the low-viscous non-composite. Nuva-Seal and of the three high-viscous composite restorative materials Adaptic, Compocap and Concise to phosphoric acid etched enamel surfaces from symmetric areas of pairs of contralateral human permanent anterior teeth. The SEM study demonstrated that the two types of material equally well reproduce the details of the enamel surface; the light microscopic observations showed that the length and shape of resin tags in the enamel surface are independent of the materials examined. The observations seem to allow the conclusion that bonding of composite restorative resins to phosphoric acid etched enamel surfaces is not conditioned by the employment of an intermediate film of a low-viscous non-composite resin.


One-hour compressive strength of dental amalgam. The one-hour compressive strength of 22 commercially available dental amalgams has been measured. Large differences between different alloys were observed. It was recognized that an intermediate one-hour compressive strength is probably the most desirable in view of the other properties which are involved.


The location of zinc in dental silver amalgam. The phases in amalgams prepared from alloys of varying zinc content and with varying alloy-to-mercury ratios have been determined by means of X-ray diffraction. By measuring lattice constants of the phases in these amalgams it has been established that the major part of the zinc is dissolved in the gamma1 phase.


Microscopic features of the lateral periodontal cyst. The lateral periodontal cyst is of developmental odontogenic origin and must be differentiated from the gingival cyst of adults, a primordial cyst in a lateral periodontal position, and a cyst of inflammatory origin. This paper is based on a histologic study of five cases. Four were in the mandibular premolar region and one in the lateral incisor-canine region of the maxilla. The cysts are lined by a thin non-keratinized epithelium which resembles the reduced enamel epithelium. Many of them arecharacterized by the presence of localized plaque-like thickenings of their epithelial linings, consisting of fusiform or large swollen, edematous cells. These epithelial thickenings appear to result from a localized proiferation of basal cells. The lateral periodontal cyst may arise initially as a dentigerous cyst which develops by expansion of the follicle along the lateral surface of the crown and comes to lie on the lateral aspect of the root if tooth eruption is normal. Alternatively, it may arise from the cell rests of Malassez.


Enamel damages caused by contracting restorative resins. Several observations have shown that contraction stresses in restorative resins bonded to the enamel of cavity walls readily broke the enamel surrounding the cavities. The forces studied were caused by polymerization contraction, thermal contraction or elastic hysteresis. The bonding was obtained mainly by etching the cavity walls by phosphoric acid.


A 3-year clinical follow-up study of pulpotomized primary molars treated with the formocresol technique. The purpose of the present investigation was to evaluate the prognosis of primary molars treated with pulpotomy employing formocresol. The material consisted of 98 primary molars. The study included clinical and radiographic observations 3, 12, 24 and 36 months after treatment. The survival rate at the 3-month follow-up was 91%, whereas the rate was 83%, 78% and 70% - 12, 24 and 36 months respectively after the treatment. The results were computed according to the life table method.


Pulp reactions in human teeth to tooth colored filling materials. In each of 49 intact premolars from children between 9 and 15 years of age, one buccal and one lingual cavity were prepared. The 98 cavities were filled with either Silicap, Sevriton Simplified or Adddent XV. After 2 weeks 19, and after 6 months 30 of the teeth were extracted. The histologic investigation included  remaining dentin, reduction in the number of odontoblasts, inflammatory cells, necrosis, tertiary dentin and bacteria. After 2 weeks a loss of odontoblasts and inflammation was seen beneath two-thirds of the cavities, and small necrotic areas in 11 of the 38 instances. After 6 months the loss of odontoblasts was a little more pronounced, the inflammation a little less, and necrosis could not be observed. Tertiary dentin was registered beneath most of the cavities after 6 months. The reactions to the three filling materials were quite similar in quality and quantity. The amount of remaining dentin influenced the degree of inflammation and amount of tertiary dentin after 6 months beneath cavities filled with Silicap, and inflammation after 2 weeks and 6 months beneath Addent. Bacteria were observed in nearly all cavities 6 months after the treatment.


Leucine and fucose incorporation into odontoblast protein. Odontoblasts isolated from rat incisors were incubated in vitro in the presence of tritiated leucine or fucose, and the amount of precursor incorporated into TCA-recipitated proteins was determined. The effect on precursor incorporation of varying the total precursor concentration was examined. The incorporation of leucine and fucose into TCA-precipitated proteins was found to be linear with time for at least 90 min. Inhibition studies with puromycin, cycloheximide and chloramphenicol demonstrated the presence of odontoblastic protein synthesis. One hour after in vivo administration of triated leucine the highest specific activity of odontoblast-predentin protein material was noted the corresponding maximum for pulp proteins was found to occur after 30 min. The in vivo incorporation of triated fucose was maximal after 4 h in both odontoblasts and pulp tissue.


Calcium fluoride in demineralized bones from rats with experimental chronic fluorosis. Thirteen male rats, 7 or 10 months old, were given 0.005% NaF in the diet or 0.025 NaF in the drinking water fro periods of 3 to 20 weeks. Femur/tibia bones were demineralized in 3.25% HNO3, the process being watched on radiographs. As soon as all radiopaque material representing mineralized bone had disappeared, demineralization was discontinued. In some instances the process was stopped while some radiopaque material was still present. Hematoxylin-eosin stained paraffin sections from all bones showed deep blue granules, fluoride granules. Transmission electron microscopy and selected area diffraction revealed that the fluoride granules contain microcrystalline CaF2. X-ray diffraction of hydrazine-deproteinated, demineralized fluorotic bones revealed the presence of CaF2 and a minor unidentified component. The results are discussed in relation to (1) the identity of the fluoride granules, and (2) their inconsistent presence in demineralized hard tissues.


The ultrastructural localization of iron in rat incisor enamel. Previous studies have shown that the pigmented surface layer of rat incisor enamel contains up to 30% iron but have left the question open as to whether iron is bound to the hydroxyapatite crystals or is present as a separate crystalline phase. Maxillary incisors from adult rats were demineralized for an extended period of time in EDTA. An insoluble membranous structure representing the remaining superficial portion of the enamel was collected and analyzed in the electron microprobe. The analyses showed that the demineralization procedure had removed calcium and phosphorus nearly completely, whereas the iron content of the surface layer had not been appreciably reduced. Electron microscopic examination of these specimens showed that the residual material contained electron-dense particles whose size, form and orientation indicated that they were remnants of the original crystal population. Several other demineralized and undemineralized specimens were also examined electron microscopically. The results indicate that iron is not present as a separate particulate phase but is, probably, bound to the surface of hydroxyapatite crystals.


Blood group antigens A and B in ameloblastomas, odontogenic keratocysts and non-keratinizing cysts. The expression of blood group antigens A and B has been studied in 8 ameloblastomas, 16 odontogenic keratocysts from patients with basal cell nevus syndrome, 11 odontogenic keratocysts from patients without the syndrome, and 12 non-keratinizing odontogenic cysts, using a double layer immunofluorescence staining technique. The amount of antigen in the lesions was compared with the content of antigen in normal buccal mucosa from each patient. All ameloblastomas reacted negatively, three cysts from the patients with the basal cell nevus syndrome reacted negatively, and the odontogenic keratocysts from patients without the syndrome as well as the non-keratinizing odontogenic cysts all gave a positive reaction.


Occlusion in the primary dentition in 3-year-old children. The material for the present study comprises plaster models of 310 Copenhagen children, impressions for which were taken during the month each child attained the age of 3 years. On the basis of fixed criteria a description is given of individual symptoms of occlusion. Using the primary canines as the guide to occlusion it was found that the same occlusion at both sides occurs in 79.7% of the overall total, the remaining 20.3% having different occlusion at each side. Approximately one-half recorded neutral occlusion at both sides, and one-third had distal occlusion at both sides. The termination pattern of the dental arches in centric occlusion were normal or straight in 68.0% and bilateral distal in 14.8%. In 82 subjects or 27% of the degree of overlapping was greater than or equal to half the height of the mandibular central incisors. The number of cross-bites constituted 11.6% of all subjects. Assessment of spacing was undertaken exclusively on the basis of whether or not a space existed. About 3.5% of all subjects had close contact between all teeth in the maxilla or crowding, and about 24% had spacing between all teeth in the maxilla. In the mandible about 5% had close contact between the teeth and only 15% had spacing between all teeth in the mandible.


Developmental disturbances in permanent teeth after exarticulation of their primary predecessors. The frequency and type of developmental disturbances in the permanent teeth in relation to the patient's age at the time of injury were examined in 77 patients with 85 exarticulated primary incisors. The central incisors are the most frequently affected teeth and injuries occur more often to teeth on the right than on the left side. The frequency of disturbed normal development of the permanent teeth was 74.1% for the total material. From 0-2 years the frequency is 94.5% and after the age of 5 years the frequency is only 18.2%. External enamel hypoplasia is the more common result in patients whose trauma occurred when they were less than 3 years old, whereas internal enamel hypoplasia is the unsual result of traumas occurring after the age of 3 years.


Traumatic injuries to enamel formation in rat incisors. Twenty-three fullgrown white male rats were fitted with an inclined plane on the mandibular incisors with consequent raising of the bite by 2.5 mm. After an observation period of between 1 and 30 d the animals were killed. In all cases traumatic injuries to the enamel organs were seen. The defects were mainly of four different types. Type 1 occurred in the early maturation zone of the enamel as a local defect with dedifferentiated ameloblasts. Vascular injury was moderate and deformation of the dentin was seen. After 30 d there was still a local defect of the enamel organ. Type 2 occurred apically to the maturation zone and caused a two-layered ameloblastoma resembling a sandwich. Hemorrhages were commmon in the surrounding connective tissue, with accompanying appearance of phospholipids in the tissue. After 30 d some restitution of the enamel formation was seen. Type 3 was necrosis was seen in the surrounding tissue. After 30 d a new ameloblastoma had developed in some areas. These three types appeared separately or combined. Type 4 occurred as a folding of newly formed apical dentin in areas without differentiated ameloblastoma and was seen in all of the experimental animals.


Effect of soluble plaque factors on inflammatory reactions in the dental pulp. The present investigation was undertaken to determine whether soluble factors from human dental plaque are capable of inducing or mediating pathological alterations in the dental pulp. The experiment was performed in three monkeys and involved 40 teeth in all: 20 test and 20 control teeth. Class V cavities were prepared on the buccal surface close to the marginal gingiva. In the test cavities an extract of human dental plaque was applied once very 5 min during an 8-h period. The cavities of the contralateral teeth were treated in an identical manner but with Ringer's solution. The animals were sacrificed 10 or 32 h after the initiation of the test. Two hours prior to sacrifice the animals were injected with colloidal carbon and then perfused with 10% neutral buffered formalin. Histologic examination demonstrates that the pulp tissue response to topical application of a plaque extract was characterized by (1) increased degree of carbon retention - indicative of vascular exudation and (2) enhanced rate of emigration of neutrophils and monocytes.


Glycoproteins in fluid from non-keratinizing jaw cysts. Fluid from 47 non-keratinizing jaw cysts and autologous serum were separated by electrophoresis on cellulose acetate membranes and stained for glycoproteins. All cyst fluids contained glycoproteins, but the separation patterns differed from those of serum. The major carbohydrate-bearing fractions of cyst fluid migrated with alpha2- and gamma-globulins, while in serum alpha2 and beta-glycoproteins contained most bound carbohydrates. The appearance of the gamma-globulin patterns indicated local synthesis and accumulation of cyst fluid gamma-globulins. The other glycoproteins most likely had escaped from plasma. Electrophoretograms of cyst fluid stained for glycosaminoglycans revealed that cystic glycoproteins often were complexed with glycosaminoglycans, causing more or less distorted separation patterns.


Effects of triamcinolone acetonide on experimental oral candidiasis in monkeys. Thirteen adult monkeys (Macaca irus) were infected with Candida albicans by inoculating the microorganisms under an acrylic plate covering the palatal mucosa. Six of the monkeys were treated with the steroid triamcinolone acetonide intramuscularly for 2 weeks before and 2 weeks after inoculation. The palatal mucosa was studied clinically and histologically at weekly or biweekly intervals for up to 5 months after inoculation. The cellular immune response was studied using the direct leukocyte migration test. In the group of seven non-steriod-treated monkeys an acute atrophic candidiasis developed that healed spontaneously in 2-3 weeks. No tissue invasion by Candida was seen in tissue sections, but the inflammation was pronounced. Migration inhibition was significant up to 5 months after infection. In the group of six steroid-treated monkeys an acute pseudomembranous candidiasis was induced that showed retarded healing, tissue invasion by Candida, and enhanced yeast proliferation. Inflammation was only slight and the peripheral blood leukocytes were not inhibited in their migration by Candida antigen. The study has shown that systemic treatment with the steroid, triamcinolone acetonide, potentiate oral Candida infections, probably by suppressing both non-specific inflammatory responses and cellular immunity.


Distribution of cadmium in developing teeth and bone of young rats. 109-Cadmium was injected intraperitoneally into 10-d-old rats. The rats were terminated at 1, 4, 24 and 96 h postinjection. Distribution of the tracer was studied by autoradiographic techniques, with particular attention directed to the dental tissues. Considerable accumulation of 109-cadmium occurred in the cells of the enamel and dentin was observed. The injected cadmium was also accumulated in the growing bone. Microautoradiography seemed to indicate that the isotope was preferentially localized in the osteoblasts.


Elution of cadmium from dentures.

Elution of cadmium from dentures. Heat-cured, pink-pigmented (light veined) Stellon maxillary acrylic denture blanks were shaken in 150 ml synthetic saliva varied from 0.15 to 1.05 mug independently of elution time. With o.1N CH3COOH the amount of cadmium, varying from 1,20 to 2.55 mug, increased slowly with elution time. Another batch of Stellon gave higher values in 0.1N CH3COOH.


Experimental periodontal breakdown in the dog. This paper describes a method for inducing rapidly progressing and reproducible periodontal lesions around selected teeth in dogs. The experiments were performed on 10 beagles which were fed a diet favoring gross formation of plaque. Approximately 1 mm of the marginal alveolar bone aroumd 4P and P4 was removed and a notch prepared in the root. A ligature of cotton floss was placed around 4P and P4 at the cemento-enamel junction. Radiographs and sections prepared 230 d later revealed that a progressive breakdown of the periodontal tissues had occurred. The average bone loss was 2.4 mm and the distance between the apical cells of the pocket epithelium and the notch was 854 mum.


Evaluation of the activity of rheumatoid arthritis. A comparative study on clinical symptoms and laboratory tests with special reference to serum sulfhydryl groups. In the present investigation the significance of various clinical and laboratory examinations (especially the S-SH-group level) in the evaluation of the activity of RA was assessed. The material comprised 140 patients with RA and 360 control patients or healthy control persons. The principal results can be summarized as follows: 1. Patients with RA have a decreased S-SH-group level, more decreased in patients with active disease. Also a change in the activity of RA is correspondingly followed by a change in the S-SH-group level. The S-SH-group level diminishes significantly with the advance of age also in healthy persons. These findings and the possible role of the S-SH-groups in the pathogenetic process of RA are discussed. 2. Highly significantly with Lansbury's activity index correlated the S-SH-groups, B-Platelets, S-Fe, B-Hb and patient's own assessment of the disease; and significantly correlated S-Haptoglobin, S-TIBC and Te-index. A summary of the correlations between some parameters studied is presented in Table LI. 3. As a result, an activity index based on six laboratory tests (ESR, B-Platelets, S-Fe, S-Haptoglobin, S-SH-groups, Te-index) is proposed for the estimation of the activity of RA.


Influence of ventilation, exercise, and body position on techniques for determining steady state diffusing capacity. Three different methods of measuring steady state diffusing capacity were analysed regarding the effect of using either the physiological dead space for CO2 (DLCOss1), the measured anatomical dead space (DLCOss2), or an assumed anatomical dead space of the lungs (DLCOss3) for the determination of alveolar CO. The material consisted of 28 healthy men and 91 patients with various pulmonary diseases. DLCOss1 and DLCOss2 were compared in the total material (Section 1) using determinations both at rest and during exercise. As was expected DLCOss1 was almost always higher than DLCOss2. The difference between the two methods followed the curve that could be predicted from the mathematically obtained relation between DL, VD/VT, and fractional CO uptake, published by West in a diagram 1962. In determinations with a large sum of the latter two variables, DLCOss1 was highly dependent on the validity of the PaCO2 used. The validity of an end-tidal sample under different ventilatory patterns is investigated in Section 2 and the relation between DLCOss and alveolar ventilation in Section 3. Relative hypoventilation gave low values for DLCOss regardless of method. There was a rapid increase in DLCOss with increasing alveolar ventilation in the normal ventilation range. During hyperventilation DLCOss1, like FuCO, decreased with increasing ventilation. The composition of the end-tidal sample was frequently distorted under forced breathing with large VT so that erroneously high VDa and DLCOss2 were computed. Consequently, DLCOss2 appeared positively correlated to VE and VT over the whole ventilation range. On the other hand, the uncertainty of the size of the anatomical dead space--assumed in DLCOss3--at high flow rates in the bronchial tree reduces the usefulness of this method during pronounced hyperventilation.


[Changes in the histochemical structures of the gingiva in the topical use of plant based drug]. If the active permeability of the oral mucosa ensures the possibility of the direct application of various substances, its influence on the intensity of metabolic processes must be detectable in the histochemical way. The present animal experiments were intended for verifying thaction of a preparation (Herbadont) on the basis of vegetable active principles on changes in the contents of ribonucleic acid, glycogen and SH and COOH groups in the gingiva. It was shown that the removal of clinical inflammatory symptoms by this preparation is associated with a decrease in the contents of COOH and SH groups, ribonucleoproteids and glucogen which are increased in case of disease. Concomitantly, the intensity of the reaction on NH2 groups and the ascorbic acid content increase.


[Some pecularities in the ultrastructure of gingival capillaries]. The authors performed electron microscopical studies on biopsy specimens of gingival papillae from subjects with clinically healthy periodontium. They observed certain peculiarities of the ultrastructure of the gingival capillaries which are indicative of a greater functional activity of the vessels in this part of the periodontium.


[Somatologic morbidity study-- Berlin 1972. 2. Incidence of periodontal diseases, condition of oral hygiene]. Using the Periodontal Disease Index and the Oral Hygiene Index, the authors determined the periodontal and oral hygiene conditions of 3853 individuals (18 to 100 years of age) selected at random from the Berlin population 67% of these individuals presented with periodontal disease; 10% were threatened by periodontal disease. The symptom "tooth loosening" was stated in 18%. The mean PDI was 2.93. The mean plaque incidence (index according to Quingley and Hein) amounted to 1.4 in subjects without periodontal disease, and to 2.2 in periodontally diseased individuals. The means supragingival calculus -- subgingival calculus ratio (index according to King) in these groups was 0.53 and 0.85, respectively.


[Lymphadenitis colli in the child from the veiwpoint of the dentist and oral surgeon]. In the light of the relevant literature and personal cases, the author deals with swellings of the cervical lymph nodes which occur most frequently in childhood. Simultaneously, he pays attention to their differentiation from odontogenic inflammatory processes in the mandibular region, which is of importance from the viewpoint of stomatology. Of 402 soft-tissue inflammations in children 0 to 14 years of age, 211 (=52.5%) were odontogenic, whereas 191 were not. There were 173 cases of cervical lymphadenitis. The aetiology, genesis, clinical picture and distribution of this disease are treated in detail as well as clinical und paraclinical symptoms which permit to differentiate them from odontogenic pyogenic infections.


[Osteomyelitis of the jaw].

[Osteomyelitis of the jaw]. A brief description of nomenclature differences and classification principles is followed by a report of the results from an analysis of cases of osteomyelitis of the jaw which have been treatedduring the years 1967-1972. During this period, altogether 745 patients with soft-tissue infection and 72 patients with osteomyelitis have been treated at the clinic. The chronic form of osteomyelitis is considerably more frequent than the acute one. The odontogenic mode of infection prevails by far. The flora of causativeorganisms and the resistance conditions are dealt with separately. The mixed flora dominates; monoinfections are rare. Antibiotherapy occupies the first place in the treatment of the acute forms whereas surgical intervention is the method of choice in the treatment of the chronic forms of osteomyelitis.


[Is there any possibility to the technic of unilateral suspended fixation?]. The ever-increasing number of traffic accidents causing fractures of the facial bones and additional complications which resulted from the rigid intermaxillary fixation may induce the operator to have recourse to other, mainly monomandibular methods. Ten years of positive experience with the technique of suspended fixation prevail on the author to apply this procedure also to unilateral fractures of the mandible. In Brno as well as in Magdeburg, the technique of suspended fixation has been found to be very efficient. The indications of this method for unilateral fractures in the region of the submaxillary body or angle are pointed out.


[Localization and frequency of interglobular dentin in human premolars from regions with various fluorine contents in the drinking water]. Using a light microscope, the authors compared 484 longitudinal ground sections of human premolars from a region where the drinking water contains 1.0 p.p.m. of fluorine withe 777 preparations from a region where the drinking water contains 0.35 p.p.m. of fluorine. Water fluoridation of fluorine supply during tooth development does not lead to a statistical significant change in the occurrence of interglobular dentine areas. Additional analyses justify at best the suspicion that teeth (and in particular female teeth) which developed under the influence of fluorine show a greater variability as to the occurrence of interglobular dentine areas.


[Radiation protection in intra-oral dental radiography]. At the time being, all dental X-ray apparatuses in the German Democratic Republic are inspected step by step on the basis of the regulations in force. An essential objective of these measures consists in ensuring that the X-ray apparatuses used comply with the standards for radiation hygiene. In this regard, the present situation is still unsatisfactory. A considerable reduction of the radiation exposure to the patients and the operating personnel, however, may be achieved easily, using simple means. An example is the restriction of the useful beam, as to which practical advice is given. Furthermore, recommendations are presented for the proper operation and for the determination of the control area required by the radiological protection regulation.


[Evaluation of the accomplishments of a state dental practice]. Starting from the great importance of morbidity and efficiency statistics to health political decisions, the author presents a critical evaluation of the services provided by a State dental practice in 1973. The stomatological primary services, i. e., those of conservative and surgical stomatology, constitute by far the greatest part of all services provided in the field of basis dental care. A comparison with data from the national and the international literature shows that these services as well as those provided in the field of prosthetic stomatology are satisfactory. Furthermore, there was a great percentage of stomatological services performed for children. - But the special consideration services provided do not and cannot meet the demands of the population. This is illustrated by an inquiry into the actual need for stomatological services per time unit.


[Ultrasound diagnosis of bone and soft tissue tumours]. The findings of ultrasonic examination in bone and soft tissue tumours are evaluated for their usefulness in preoperative tumour diagnosis, with particular reference to size, demarcation, structure, as well as the topographic situation of the tumour. Furthermore, the results of ultrasonic tomograms are compared with those of X-rays and angiography. It seems that this simple method, which is without risk to the patient, is a valuable additional investigation in the diagnosis and therapy of tumours of bone and soft tissue.


[A misleading diagnosis of ovarian tumours in a case of paramyeloblastosis (author's transl)]. A case is reported of bilateral ovarian tumours in a 44 year-old patient, who was submitted to total hysterectomy with removal of both appendages. Preoperative laboratory investigations had not yielded any useful information. Histologically, both ovaries showed replacement of the normal tissue by sheets of undifferentiated tumour cells. A sudden rise in temperature with rigors occurred on the 8th postoperative day, in conjunction with a considerable degree of anaemia, the appearance of ascites and a massive increase in size of the liver. Shortly afterwards, 50% of the peripheral leucocytes were found to be paramyeloblasts, the total leucocyte count being 4300. Sternal puncture confirmed the diagnosis of paramyeloblastosis, which proved rapidly fatal in spite of intensive therapy with corticosteroids, antibiotics and blood transfusions. The typical picture of paramyeloblastosis with tumour formation was observed on post-mortem examination, with leukaemic invasion of the liver, spleen, lymph glands and bone marrow.


[The "juvenile" type of chronic myelogenous leukemia (author's transl)]. The juvenile type of chronic myelogenous leukemia (CML) is characterized by hemorrhagic diathesis, hepatosplenomegaly, generalized enlargement of lymph nodes, reduced erythro- and thrombopoiesis, leukemic infiltration of bone marrow, and decreased activity of leukocyte alkaline phosphatase. The disorder differs from the adult type by the early manifestation of anemia and thrombocytopenia, the minor degree of leucocytosis, the failure to demonstrate the Philadelphia chromosome, and the presence of fetal markers in the patients' erythrocytes. The persistence of fetal markers in erythrocytes and the clinical manifestation in infancy and early childhood suggest that the disease is congenital in origin. The occurrence of CML of the juvenile type in an 8-year-old boy, which is reported in this paper, favors a postnatal acquisition of the disorder. The significance of hematological and cytogenetic findings and new aspects of nosological classification are discussed.


[Incidence of coeliac disease in children in Austria (author's transl)]. The incidence of coeliac disease in infants and children was estimated by the number of children born between 1 January 1969 and 31 December 1972 and the number of patients with coeliac disease born during the same period in the area served by the Kinderklinik Graz. The drainage area of the Kinderklinik Graz comprises 10.5% of the population of Austria and 11.1% of the children born in Austria during the period mentioned. The diagnostic criteria for coeliac disease includes evidence of intestinal malabsorption, a subtotal atrophy of the duodenojejunal mucosa and the demonstration of clinical and biochemical response to gluten withdrawal. In all patients born in 1972 a second biopsy was done after a period on gluten free diet and a third biopsy after reintroduction of gluten. The incidence of coeliac disease showed to be 1 in 496. The incidence for girls proved to be 1 in 403 and that for boys 1 in 636. These figures are in good correlation to that reported for Ireland and Switzerland but much higher than those estimated for Britain.


Studies on small intestinal obstruction. The results of the present study can be summarised by the following answers to the framed questions: (I) Simple small-bowel obstruction in the cat entails predominantly a distension of the intestine, whereas the sustained intraluminal pressure is rather modest, between 5 and 10 mmHg. Spontaneous bowel activity is still present after 72 hours and responds to pharmacologic stimulation (II) Haemodynamic and microcirculatory evaluation in vitro shortly after release of an in vivo obstruction reveals no difference between obstructed and non-obstructed intestine; after decompression, thus any impairment of bowel "viability" caused by obstruction is rapidly ameliorated. (III) A moderate distension of short duration increases regional vascular resistance in non-obstructed intestine but compromises neither capillary filtration rate nor oxygen consumption. Upon a further and considerable distension, both haemodynamic and microcirculatory variables deteriorate in parallel; at an intraluminal pressure of 100mmHg, 30% of the blood flow and probably less than 15% of the perfused capillary surface area are preserved. Thus, the intestinal microcirculation is not interrupted until an 'unphysiologic' distention is applied. (IV) After release of an in vivo obstruction, a considerable distension produces the same circulatory responses as those recorded in non-obstructed intestine, whereas a moderate distension compromises the capillary function in obstructed but not in non-obstructed intestine; it is suggested that this is mainly the result of enhanced bowel-wall distensibility in obstruction. (V) A moderate and sustained distension occasions a 50-60% resistance augmentation in non-obstructed bowel and a 45-50% reduction of the perfused capillary surface area but does not compromise the oxygen uptake; thus, moderate distension involves no threat to bowel microcirculation or 'viability'. The experimental procedure per se entails some degree of resistance augmentation and CFC reduction during a 3-hour perfusion; however, no changes appear during the initial stage, i.e., corresponding to the period of artificial distension...


Changing cleft widths: a problem revisited. A study was undertaken to evaluate changing cleft widths and the timing of surgical repair of the lip in so far as it affects the types of cross-bite occlussion that result in cases treated by presurgical orthopedics (as practiced at Tufts-New England Medical Center Hospitals) and by purely surgical intervention. On the basis of the analyses carried out, the following conclusions were reached: 1. Both the alveolar cleft width prior to lip surgery and the arch form following lip surgery are significantly related, and are predictors of, the type of cross-bite occlusion that will result from purely surgical intervention. 2. Neither the alveolar cleft width prior to lip surgery nor the arch form following lip surgery is significantly related to, or is a predictor of, the type of cross-bite occlusion that will result from presurgical orthopedics. 3. For both presurgical orthopedic treatment programs and purely surgical treatment programs, there is a significant relationship between the arch form following palate surgery and the type of cross-bite occlusion that will result. 4. In cases treated by presurgical orthopedics there is a higher probability of achieving favorable occlusal relationships than in cases treated by purely surgical intervention. 5. In a period of 6 months following birth, but prior to lip surgery, presurgical orthopedics results in a smaller percentage reduction in alveolar cleft width than does treatment without the use of an appliance. However, the posterior cleft widths and posterior palate widths show comparable changes in size. 6. The larger anterior cleft width maintained by the use of presurgical orthopedics prior to lip surgery is completely compensated for following lip surgery, so that no significant difference remains between cases treated by presurgical orthopedics and those treated by purely surgical intervention. 7. Since previous findings showed a larger reduction in posterior cleft width in cases treated by presurgical orthopedics than in cases treated by purely surgical intervention and these results show that there is a comparable reduction in posterior cleft width when lip surgery is delayed until the age of 6 months, then early lip surgery tends to limit the natural reduction of the posterior cleft width. 8. Additional studies are needed. These studies must be based on as complete a description of the cleft and the treatment program as possible. It is the total implication for patient rehabilitation that must be considered in the evaluation of treatment outcomes.


Orthodontic positioner used for home fluoride treatments. It has been well documented that the more time the enamel surface is exposed to fluoride, the higher will be the caries protection. An extremely good method of greatly increasing caries resistance is by daily or weekly home fluoride treatments. This procedure is usually limited to special cases of rampant caries, patients with hemophilia, and handicapped patients in whom caries control is vital. It is not used more widely because of the added costs of impressions, tray fabrication, and professional. If the patient already has a vehicle for applying fluoride, such as a positioner or mouth guard, it is a simple matter to prescribe or dispense fluoride gel to be used when these appliances are being worn. This is expecially true for orthodontic postioners. When bands are removed, a slight space usually exists interproximally. This space greatly facilitates fluoride contact with the proximal surfaces. Fluoride will tend to recalcify decalcified areas of enamel in addition to providing added caries protection for the patient.  If positioners are not used after band removal, home fluoride trays or rinses can also be effective. Since many orthodontic patients wear a positioner when bands are removed, the orthodontist can provide his patients with the benefits of topical fluoride by prescribing fluoride gel and explaining how to use it along with his positioner.


Variability of the craniofacial skeleton.  III. Radiographic cephalometry of juvenile Macaca mulatta. The variability of the craniofacial skeleton of the juvenile Macaca mulatta was studied from lateral radiographic cephalograms of twenty-three animals, using a previously described method based on fourteen lines depicting certain anatomic features.  The findings, when compared to corresponding human data, revealed that a number of angular relationships are similar in this monkey and in human childrena and adults.  The same is true regarding the variability of individual lines.  These similarities suggest that biologic similarities exist in the cranio-facial skeletal architecture in these species.  Specifically, the findings in the Macaca mulatta were in agreement with previous findings in human children and adults as regards the parallelism between the anterior parts of the neurovascular canals of the upper and lower jaws and the apparently adaptive role of the ramus and the condyle.


Palatal growth studied on human autopsy material.  A histologic microradiographic study. The postnatal development of the hard palate was studied by conventional histologic and microradiographic means on autopsy material from thirty-three boys and twenty-seven girls aged 0 to 18 years.  The findings indicated thet growth in length of the hard palate until the age of 13 to 15 was due to growth in the transverse suture and to apposition on the posterior margin of the palate.  After this age the sutural growth was found to cease, whereas the apposition seemed to continue for some years.  During the postnatal development the morphology of the transverse suture changed.  At birth the suture was broad and slightly sinuous; later it developed into a typical squamous suture, the palatine part covering the maxillary part.  During puberty the course of the suture was again slightly sinuous.  The importance of this change for the vertical growth of the hard palate was discusses.  It was pointed out that the lowering of the anterior part of the palate.  The transverse growth of the midpalatal suture continued up to tha age of 16 in girls and 18 in boys.  On the basis of morphology, the development of the median suture could be divided into three stages.  In the first stage the suture was short, broad, and Y shaped; in the second the course was more sinuous; and in the third interdigitation was so heavy that a separation of the two halves of the maxilla would not be possible without fracturing the interdigitated processes.


["Narcose à distance" for pneumoencephalography using the x-ray apparatus neurocentrix (author's transl)]. A method of anesthesia for pneumoencephalography patients in combination with a modern automated x-ray apparatus (Neurocentrix, C.G.R.) is presented with all technical and apparatus details. The method was used in 62 patients.


Complications of BCG vaccination in neoplastic disease. More than 300 patients with neoplastic disease (most with lymphoma or leukemia) were given one or more BCG vaccinations. In the great majority of cases, these were well tolerated. The complications we observed were of three types: (A) persistent BCG infection that could disseminate widely, (B) activation of old, dormant acid-fast infection, and (C) hypersensitivity reactions. The latter were related to the frequency of administration and dose of organisms. It is important to recognize that BCG organisms may not be completely destroyed by patients with impaired immunologic defenses, and that their inoculation may create a source of disseminated acid-fast infection at a later time. In our limited experience, treatment with isoniazid for 2 to 3 months has resulted in cure of BCG infection. Hypersensitivity reactions may constitute a more serious problem than such infections.


Drugs five years later: cytarabine.

Drugs five years later: cytarabine. Cytarabine is an effective agent in the treatment of acute leukemia. Since its approval by The Food and Drug Administration in 1969, the clinical effectiveness of this drug has increased as knowledge of its pharmacologic and biologic properties has been translated into clinical trials. A complete remission rate of greater than 50% can be achieved when cytarabine is used in combination with other agents in the treatment of adult acute myeloblastic leukemia. Remissions occur only after the development of significant bone-marrow hypoplasia, and the care of patients through this period of pancytopenia requires elaborate supportive techniques and facilities. The role of cytarabine in the treatment of acute lymphoblastic leukemia and lymphoma is still under clinical investigation and appears promising. Because the clinical effectiveness of cytarabine in the treatment of nonmalignant diseases has not been proved, its use in these disorders must be considered investigational and weighed against the serious bone-marrow suppression and potential long-term hazards of this drug.


[Blastic crisis and cerebral leukotaxis in a case of lymphoblastic acute leukemia]. The present report deals with the case of an eleven-years-old child with acute lymphoblastic leukemia who showed blastic crises, leukostasis and cerebral hemorrhage. The pathogenesis of these complications is analyzed and some therapeutic rules are outlined.