[Fluorescence and electron-microscopic analysis of differentiation of the myocytes of the ventricles and atria of the avaian heart in ontogenesis]. The histogenesis of the ventricle and atria myocardium was studied in 240 chick embryos of from 30--34 hours up to 20 days of incubation and in 66 chickens at the age of 1--90 days. Fixators were the Bouin's Carnoy's and Newkomer's fluids and the 100% solution of neutral formalin. The paraffin sections were stained by histological and histochemical methods. RNA, DNA, glycogen and lipids were detected. The ultrafine sections of the material fixed in 1% buffer solution of osmium tetroxide (pH 7,2) and embedded in araldite were prepared for electron microscopy. The contrasting was made in solutions of acetic uranium and lead citrate. Electron microscope--UEMB-100K, accelarating voltage--75 kV. The prefunctional period of cardiogenesis is characterized by most intensive processes of specific differentiation of cardiac myocytes and by accumulation of energetic material. With the growing functional activity of the tubular heart the amount and size of lipid drops in the cytoplasm of differentiating cardiomyocytes diminished suggesting the use of lipids as the main source of energy. In the period of formation of the heart the trabecular myocardium of ventricles and the atria myocardium, seeming to perform the main functional load, are characterized by a better developed contracting apparatus and a considerable content of glycogen granules. A sharp decrease of the glycogen content at the end of the embryonic period is likely to be due to growing hypoxia, the appearance of lung respiration and transition to the other type of energy metabolism.


[Changes in the lymphoid tissue and in the level of corticosteroids in staphylococcal intoxication and infection]. A complex quantitative study of changes of proliferative processes in lymphatic vessels on the one hand and changes of the level of corticosteroids in the peripheral blood in staphylococcal intoxication and infection in the same rats on the other hand has been carried out. In the work H3-thymidine has been used. The comparison of the obtained morphological data with the dynamics of the change in the corticosteroid level shows that the decay of lymphocytes, the devastating of light centers and the cortical substance of lymph nodes, an abrupt lowering of the amount of DNA-synthesizing and mitotically dividing cells take place in the period associated with a release of a great amount of corticosteroids into the blood. The drop of their level to control values goes in parallel with increasing the amount of DNA-synthesizing and mitotically dividing cells. The obtained data suggest that the leading role in those changes of the lympoid tissue which had been described by a number of authors in infectional processes is played by corticosteroids.


[Adaptive characteristics of growth of human embryos and fetuses during the intrauterine life]. Under observations was the dynamics of growth of human embryos and fetuses in embryogenesis which is directly dependent on the month of the intrauterine life. Coefficients of correlation and regression were calculated and with their help the average growth of the general length of fetuses in each month of prenancy was determined. The enlargement of the fetus length was found to be more intensive in the embryonic and prefetal periods.


[Ultrastructure of heart synapses].

[Ultrastructure of heart synapses]. The nerve endings in the intramural ganglia of the rat's heart are connected by synaptic and non-synaptic junctions with the dendrites and neuron bodies. Peri-membrane indurations of synaptic complexes may look relatively symmetrical or asymmetrical depending on the orientation of the section in relation to the elements of the Akert's presynaptic lattice. In non-synaptic junctions the indurations are symmetrical, but the presynaptic one may be more complicated in its structure. The synaptic complexes are disposed in the field of synaptic dilatations of axons and in the sites of interlacing thin "preterminal" parts of axons with dendrites. They connect preganglionic fibres with dendrites, neuron bodies and with the filamentous and fungiform thorns.


[Causes of rupture of formalinized xenovalves in the post-transplantation period]. The article discusses results of the morphological study of xenografts of the pig's valves fixed in 0.32% and 4% solution of formaldehyde which remained in the human heart during the period ranging from 21 months to several hours. In the xenovalves there occurred progressive destructive changes in their connective-tissue basis, which led to weakening of the mechanical strength and to rupture of the cusps. In the majority of cases, no morphological signs of immune or nonspecific inflammatory cellular reaction were observed. The authors hold that polycondensation of protein molecules occurring in the course of preservation under the effect of formaldehyde, and formation of "superstrong" structures at the expense of increased number of transversal connections between collagenous fibres not always could ensure sufficient mechanical strength of the cusps of the graft.


[Destruction of the aortic allo- and xenovalves of the heart depending on their position and time of transplantation]. The aortic valve, as well as the other valves of the heart, has a cellular structure. Collagenous fibres form the skeleton of the cellular structures, and the plexus of thinner fibres--the "walls". At early periods following the implantation destruction of thin fibres distributed along the periphery of the alveolus takes place. In the course of prolonged functioning of the allo- and xenovalves in a mitral position the large collagenous fibres collapse, defibration thereof develops and slits-vacuums and "cysts" are formed. In the valves which functioned for a long time in the tricuspid position no considerable destructive changes in the connective-tissue carcass take place.


[Change in the ultrastructure of the muscles in thyrotoxic periodic paralysis]. The article presents data on the clinico-morphological study of three patients suffering from thyrotoxicosis with attacks of periodic paralysis. The muscles obtained by biopsy in two patients at the moment of attack and in one patient--in the absence of attack were investigated by the electron microscopy method. The data obtained make it possible to assume that in the thyrotoxic periodic paralysis the action of the thyroid hormones causes not only impairment of the mineral metabolism, but brings about changes in the structure of the membranes of the sarcolemma and T-system, which leads to disturbances of conductance of action potential into the fibre. These changes affect the function of the end cisterns and lead to distorsion of the processes of conjugation of excitation-contraction with resulting development of paresis and paralysis of muscles. Moreover, during the attack there take place secondary disturbances of carbohydrate metabolism, which aggravates the pathological process.


[Diagnostic analysis of biogenic amines in tumors]. The modifield Falck's fluorescence-microscope method was used to investigate the content of biogenic amines in 218 bioptic specimens taken from patients suffering from tumours and other pathological conditions. It was established that malignanat tumours were characterized by accumulation in the tissue of the tumour and particularly in the reactive zone, of considerable amounts of substances with green luminescence (histamine, catecholamines, and probably, di- and polyamines) and by the disappearance of serotonin from the connective-tissue cells. In benign tumours, as well as in focal processes of non-tumour character (chronic gastric ulcer, mastitis, pulmonary tuberculosis) in the reactive zone there were always an increased number of connective-tissue cells containing serotonin. An opinion was put forward to the effect that one can judge about a degree of lesion of the connective tissue and the nature of the course of a pathological process from the character of biogenic amine contained in the elements of the connective tissue in the reactive zone. Identification of biogenic amines in the tissue of the reactive zone may serve as an additional test in carrying out differential diagnosis and will be of use in differentiating malignant tumours from benign processes and other focla pathology of non-tumour nature.


[Autoradiographic study of cancer of the rectum before and after radiotherapy]. Prolifirative activity of rectum cancer cells was investigated with the help of 3H-thymidine in 13 patents before and after radiotherapy in doses 2000-4000 rad. It was established that index of the label depended upon the degree of differentiation of a tumour and was in the range of 10.3 to 21%. Preoperative irradiation in a dose of 2000 rad caused a slight decrease in the label index (8.7-19%), but affected markedly the intensity of inclusion of the preparation. Radiotherapy in a dose of 4000 rad brought about a well pronounced reduction in the label index (3.1%) and in the intensity of labelling.


[Cellular contacts and "cytoplasmic bridges" in the inflammation focus (scanning and transmission electron microscopic study)]. Contacts between cells in an inflammation focus were studied by the methods of scanning (raster) and transmission electron microscopy on a model of aseptic inflammation in the subcutaneous connective tissue in rats. It is shown that contacts among the cells of the connective tissue and the blood in the form of "cytoplasmatic bridges", observed in the scanning electron microscope, were not confirmed by the studies in the transmission microscope. The investigation of the "cytoplasmatic bridges" in the transmission  electron microscope revealed a close adjacence of the cytolemma of the contacting cells, or a harrow space between them as wide as 10-30 micron.


[Diffuse polyposis of the large intestine complicated by amyloidosis of the internal organs]. Polyposis of the large intestine is a common affliction of the intestine which usually occurs as a result of inflammatory diseases or of dysplasia of embryonic anlages. Malignization of diffuse polyposis is observed in 70-100% of cases. A case of diffuse polyposis of the large intestine with initial phenomena of malignization in a male patient aged 44 years is described. The case in question was characterized by the presence of accompanying diffuse amyloidosis of a number of internal organs (kidney, spleen, pancreas). The onset of amyloidosis in this case was associated apparently with the character of absorption by a pathologically changed surface of the large intestine mucosa and with the enlargement of the surface of absorption of products of the protein metabolism.


[Atypical form of hemochromatosis].

[Atypical form of hemochromatosis]. A rare case of an atypical course of hemochromatosis in a woman at the age of 25 years is described; it was characterized by predominant lesions of the cardiac muscle, liver, pancreas, by phenomena of cardiovascular incompetence, by the absence of signs of melanodermia.


Alterations in lipid metabolism produced by ethyl trichloracetate. Rats maintained on a choline deficient diet and treated with subcutaneous doses of ethyl trichloracetate responded by increasing plasma beta-lipoprotein and plasma triglyceride levels while excess triglyceride was being removed from the liver. There was a transient depression in plasma phospholipid at the beginning of the treatment. Continued administration of ethyl trichloracetate raised plasma triglyceride in choline depleted rats and raised hepatic phospholipid concentration in both choline deficient and supplemented rats. It is suggested that the lipotropic action of ethyl trichloracetate occurs through hepatic triglyceride being removed by the altered plasma lipids and not by inhibition of hepatic triglyceride synthesis.


Hepatic vitamin A fat-storage cells and the metabolism of chylomicron cholesterol. These experiments were designed to test the hypothesis that the vitamin A fat-storage cell removes chylomicron remnant cholesterol from hepatic portal venous blood; A modified Ficoll density gradient ultracentrifugation procedure was used to isolate from rat liver cellular fractions that were enriched in vitamin A. In rats fed a normal diet and in rats fed excess vitamin A isolated hepatocytes were fractionated 15 min after the intravenous injection of chylomicrons labelled in vivo with radioactive cholesterol. The results showed that cholesterol radioactivity was not concentrated in the vitamin A enriched cellular fractions, so it was concluded that the vitamin A fat-storage cell is not implicated in clearance of chylomicron remnants by the liver.


The origin and significance of macrophages in thoracic duct lymph. Efferent lymph collected from a thoracic duct fistula initially contains no macrophages. However, the surgical procedures used to insert plastic cannulae into efferent lymphatics incite a vigorous foreign body reaction leading to the contamination of collected lymph with significant numbers of these cells. A sensitive and specific assay for the presence of macrophages in lymphocyte populations was used to quantitate the degree of contamination in rats bearing thoracic duct cannulae. The origin of some of these contaminant cells from the peritoneal macorphage population was established by adoptive transfer of labelled peritoneal cells to cannulated recipients.


Host resistance to a spontaneously metastasising melanoma after primary tumour excision. Tumour resistance was investigated in a spontaneously metastasising murine melanoma. Specific tumour resistance was found in mice with intact small primary tumours and no metastases. If primary tumours were excised, resistance was demonstrated in mice which had metastases and in mice which did not have metastases but were given crude melanoma homogenate. No resistance could be detected in mice which had large primary tumours left intact or in mice which had small primary tumours excised four days prior to tumour challenge. These in vivo findings are compared with previously documented in vitro results.


Mechanism of thyroid allograft rejection. Balb/c thyroids, held in organ culture for 26 days, survive and function as well as isografts for greater than 100 days in CBA recipients. Uncultured allografts are totally rejected by 20 days after transplantation. Prolonged allograft survival can also be achieved by the treatment of donor animals with cyclophosphamide prior to harvesting tissues for transplantation. These allografts do not survive as well as 26 day cultured allografts, but cyclophosphamide pretreatment reduces the culture time required to achieve indefinite survival to 7 days. The provision of an allogeneic (LD) stimulus by thyroid tissue that is I-region incompatible with the host does not facilitate the rejection of a tolerated cultured allograft. However, activation of the host immune system by an uncultured graft syngeneic to a tolerated cultured allograft leads to the chronic rejection of the cultured transplant. The transfer of a tolerated cultured allograft back to its strain of origin induces an acute inflammatory reaction that causes tissue damage within the transplant but does not lead to the total destruction of the tissue.


Studies on the transfer of plasma iron to milk in the lactating rat. The transfer of an intravenous does of 59Fe from plasma to milk in the lactating rat was a rapid process which accelerated exponentially with time until a plateau was reached 2 to 4 hours after injection. This transfer of iron involved little participation of the iron in the mammary gland pool. Also, neither the lactoferrin of the milk whey nor the iron-carrier molecule of the casein played a specific role in the iron transfer process. The present results show that the total percentage of plasma 59Fe transferred to the milk and sucklings in the lactating rat is much more than those values reported in lactating rabbits (Tarvydas, Jordan and Morgan, 1968) and sows (Hansard, 1965).


[Synthesis of iodoamino acids during in vitro thyroglobulin iodination in different states of its molecule]. Iodotyrosine and iodothyronine residues are formed in the protein molecule during bovine thyroglobulin iodination in vitro. Dissociation and reassociation of the thyroglobulin molecule have no significant influence on its iodoaminoacid composition. Thyroglobulin iodination in the presence of 8 M urea does not result in thyroxine synthesis  despite the increased formation of iodotyrosine residues. Similarly, during iodination of reassociated thyroglobulin the new molecules of thyroxine are not formed either. It is presumed that during reassociation of thyroglobulin subunits the native conformation of the protein is not completely reconstituted. The results obtained suggest that the structure of thyroglobulin controls the distribution of the iodine atoms incorporated by the iodoaminoacid residues.


[Stimulating effect of histones on DNA synthesis in the regenerating rat liver]. Effect of exogenous histones, nuclear globulins and acid proteins on DNA synthesis is studied in regenerating liver of rats in which the synthesis of their own proteins and thus DNA replication are inhibited by cycloheximide. In these conditions histones from regenerating rat liver are found to stimulate 3H-thymidine incorporation into DNA of hepatectomyzed rat liver. Nuclear globulins and acid proteins from regenerating liver, and histones from intact liver produced no stimulating effect on DNA sythesis.


[Purification and certain properties of pyruvate decarboxylase from bovine brain]. A procedure of isolation and purification of pyruvate decarboxylase (PDC) from bovine brain is worked out. 350-fold purified enzyme preparation was homogenous under polyacrylamide gel electrophoresis. Molecular weight of PDC from bovine brain was estimated to be 180 000 by means of gel chromatography through Sephadex G-200. The protein was eluted in two peaks (with molecular weight of 180 000 and 90 000 respectively). After the treatment of the enzyme preparation with 6 M guanidine chloride. Probably, partial dissociation of the enzyme molecule into two subunits takes place in this case. Data on paper chromatography confirmed that highly purified PDC preparations from bovine brain were isolated as apoenzyme, since they were almost free of TPP.


[Effect of hydrocortisone on methylation and molecular population of DNA in rat liver]. The content of 5-methyl cytosine in rat liver DNA increases 1,7-fold 8 hours after intraperitoneal injection of hydrocortisone (5 mg per 100 g animal weight). The content of GC, physicochemical parameters (Tm, delta T, etc.) and DNA renaturation pattern did not show any changes. No changes were observed in the pattern of H3-thymidine incorporation into rat liver DNA: after hydrocortisone injection the radioactivity was found to be equally distributed in all isolated sequences of DNA, differing in the degree of reiteration (specific radioactivities of these DNA, fractions are very similar). Thus, the molecular population of DNA in liver cells remains unchanged, which suggests that the hormone-induced increase in the 5-methyl cytosine content is due to a change in the DNA methylation level. The methyation level of unique sequences (COt greater than 600), i. e. that of structural genes, does not undergo any essential changes. The reversible methylation of DNA regulated by hormones seems to be one of the mechanisms controlling gene activity.


[Purification and properties of high-molecular-weight rabbit kininogen]. A preparation of high-molecular weight kininogen (HMWK) was isolated from rabbit citrate blood plasma and purified using chromatography on DEAE-Sephadex A-50 and CM-Sephadex C-50. From 1 mg HMWK, trypsin or kallikreine of human blood plasma release 10 mkg bradykinine. The HMWK preparation is homogeneous during electrophoresis in 7.5% polyacrylamide gel in tris-glycine buffer, pH 8.3; its electrophoretic mobility corresponds to that of alpha2-globulins. The molecular weight of HMWK estimated using the collumn with Sephadex G-200, is 130.000--150.000; the sedimentation constant S20w is 7.6. Rabbit HMWK is neither a dimer, nor a trimer of low molecular weight kininogen (LMWK), since it does not degrade into subunits after treatment by 2.5% solution of sodium dodecyl sulfate, containing 8 M urea. 0.05 M 2-mercaptoethanol and 8 M urea induce HMWK splitting into 2 fragments with respective molecular weights of 80.000 and 30.000, the kinine-containing group being localized in the low-molecular weight fragment. Estimation of rates of kinine formation by different kininogenasses from highly purified HMWK and LMWK preparations showed that those kininogens are functionally different substrates, since blood plasma kallikreines release kinines from HMWK at a greater rates, whereas tissue kallikreines, e. g. human saliva kallikreine release kinines from LMWK. The specificity of kallikreines as kininogenase, to trypsin, was determined. Tripsin removes bradykinine from both kininogens at the same rates, which are an order of magnitude less than those found for kallikreines.


[In vitro methylation of nuclear DNA from various organs of a rat: Tissue and age differences in acceptor ability of DNA]. DNA-methylase activities have been found, isolated and partially purified from the nuclear and cellular extracts of rat liver, kidneys, spleen, brain and lungs. These metylases are capable of methylating in vitro homologous and heterologous DNA from rat tissues in the presence of (H3-methyl)-S-adenosylmethionine. The radioactive methyl groups incorporated in vitro into DNA were found only in 5-methylcytosine. No radioactivity was revealed in either N6-methyladenine or N6-dimethyladenine. Rat spleen DNA-methylase differs in some properties (pH and temperature dependences) from those of DNA methylases from other rat organs. This enzyme methylates various DNA to a higher extent than DNA-methylases from other rat tissues. There may exist a tissue specificity of DNA-methylases from other rat tissues. There may exist a tissue specificity of DNA-methylases and a different mode of their action and recognition of nucleotide sequences. The nuclear DNA from various rat organs methylated in vitro by the same enzyme possesses a different ability to accept methyl groups from S-adenosylmethionine: there exists a tissue specifity of DNA methylation in vivo and in  vitro. The degree of DNA methylation changes with age tissue specifically. Aging is accompanied with decrease in the 5-methylcytosine content of DNA of some tissues (brain and others) and increase in methyl-accepting capacities of these DNA in the in vitro methylation by home- and heterologous DNA-methylases. The age changes detected in DNA are correlated with exhaustion of tissue functional activity with age and support our previous suggestion that DNA methylation is a mechanism for regulation of transcription and gene activity.


[Study of the membranes of pigment-free mutant of Staphylococcus aureus]. The membranes from pigmented strain of Staphyloccus aureus 209-P and its apigment mutant B-6 were isolated. The infrared spectra, the cytochrome content, and the activities of the dehydrogenases and oxidases of the membranes were studied. The apigment mutant membranes differed from those of the pigmented strain in all parameters studied. It is concluded that the carotenoid pigments affect the chemical composition and functional activity of the Staphylococcus membranes.


[Aspartate transaminase from cytosol of the chicken muscles: Its purification and various properties]. Homogeneous cytosolic aspartate aminotransferase was prepared from chicken muscle. The purification procedure involves heat and ammonium sulfate fractionation, chromatography on ion-exchage cellulose CM-52 and crystallization of the enzyme. A comparison of some properties of aspartate aminotransferases from chicken skeletal muscle and heart has been made. Both enzymes were found identical in terms of their electrophoretic mobility, isoelectric point, pyridoxal phosphate content and the amount of SH-groups.


[Effect of the conditions of isolation and incubation of the nuclei on digestion of DNA chromatin by calcium- and magnesium-dependent endonuclease. Change in the spectrum of chromosomal proteins and possible role of DNA-protein interactions]. Digesting of chromosomal DNA of interphase rat liver nuclei by Ca, Mg-dependent endonuclease in situ in the presence of chelating agents results in the appearance of the soluble DNP--up to 30% of the total DNA. In addition, 50% of the chromatin is solubilised after mild ultrasonication. In the absence of the chelating agents the degree of fragmentation is considerably increased. The process is accompanied by a loss of some histone and nonhistone chromosomal proteins; the nonhistone proteins are lost selectively. The preliminary removal of the nuclear membrane and significant part of the proteins by tritone X-100 promotes the chromatin degradation and the appearance of low molecular weight fragments. The DNA-fragments of solubilised chromatin are similar to the DNA-fragments of residual chromatin, but in the presence of the chelating agents the latter does not contain monomeric fragments.


[Isolation and certain properties of human pituitary prolactin]. A simple method of isolation of highly purified prolactin from acetonated preparations of anterior hypophysial lobes is described. The new method permits to obtain higher (about 10-fold) yields of the hormone, as compared to those obtained using previously described methods. Prolactin was extracted by acid aqueous acetone and was subsequently purified of extract by fractionation with acetone and NaCl and by isoelectric precipitation. The final stage of the hormone purification involved gel-filtration through Sephadex G-200; prolactin yield was 400 microgram per 1 hypophysis. The lactogenic activity of the hormone is 14 MU/mg; the sequence of N-terminal amino acid residues of prolactin is as follows: NH2-Leu-Pro-Ile-x-Pro-Leu(?)-Gly-Ala-.


[Selectivity of lysozyme oxidation by singlet oxygen]. Peptide analysis of tryptic hydrolysates of two lysozyme forms derived from oxidation of lysozyme with singlet oxygen shows that Trp-62, located at the active site, is destroyed. This is confirmed by the protective effect of the substrate (chitin), whose presense practically prevents the oxidation. A possibility of oxidating different tryptophan residues is discussed from the view-point of their availability to the reagent.


[Study of reactivity of sulfhydryl groups of troponin]. The reactivities of SH-groups of troponin and its components were studied, using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). At low concentrations of Ca2+ (pCa greater than 8) one rapidly- and two slowly-reacting SH-groups of troponin I and one SH-group of troponin C slowly reacting with NBD-chloride are titrated in the whole troponin complex. When Ca2+ concentration is increased up to pCa less than 5, only one slowly-reacting and one rapidly-reacting with NBD-chloride SH-groups of troponin I are titrated. The increase of Ca2+ concentration from pCa greater than 8 to pCa less than 5 results in a change of the environment polarity for the highly reactive SH-group of troponin I in the whole troponin complex. This phenomenon may suggest that the changes in troponin C structure during Ca2+ binding somehow induce changes in that of troponin I. The half-maximal change of troponin SH-groups reactivity is found at pCa 6.8. No cooperativity for Ca2+ binding by the troponin complex is observed using SH-groups titration by NBD-Cl.


RNA synthesis in nuclei of rat liver and of human lymphocytes. Physico-chemical properties and RNA synthesis in the rat liver and human lymphocytes have been compared in a nuclear system in vitro. Human lymphocytes were isolated from blood of healthy donors and of chronic lymphocytic leukaemia patients. The isolated nuclei served as the source of polymerase and template DNA. 3H-CTP was incorporated into the acid insoluble fraction linearly for 60 min. The nuclei of lymphocytes contained small amounts of RNA and protein, and the isolation procedure was complicated. Rat liver nuclei seem to be less prone to clumping at high pH values and may incorporate much more 3H-CTP. The nuclear synthesis was compared with incorporation of 3H-rU and 32P-orthophosphate into nuclear RNA of intact lymphocytes. Normal cells easily incorporated 32-P, and in contrast leukaemic cells incorporated 3H-rU to a greater extent.


Effect of aminonucleoside on the stability of rat liver ribosomal RNA to heating. High molecular weight ribosomal RNA has been isolated from normal rat liver and from the livers of rats treated with aminonucleoside. Melting curves of the rRNA from treated rat livers were shifted to higher temperatures. The melting temperatures were found to be 62.5 and 66 degrees C for normal and treated material respectively. The rRNA was heated at different temperatures and analysed by polyacrylamide gel electrophoresis. In both normal and treated material, the 18 S was more stable in the treated material when compared with the control material.


Chloroplast fine structure in the japonica-2 maize mutant exposed to continuous illumination. 1. The green tissues. Exposure to continuous illumination causes the appearance of numerous plastoglobuli in the stroma of both the mesophyll and bundle sheath chloroplasts of the green tissues of the leaves of the japonica-2 mutant of maize. In the pale green tissues the thylakoids have markedly swollen membranes. Another feature of the plastids exposed to continuous illumination is the heavy accumulation of starch. The japonica-2 chloroplasts show a different sensitivity to light, the chloroplasts of the pale green tissues being affected more markedly than the ones of the dark green tissues, and the bundle sheath chloroplasts more than those of the mesophyll. The effects of continuous illumination may be interpreted as an acceleration of chloroplast ontogenesis.


Chloroplast fine structure in the japonica-2 maize mutant exposed to continuous illumination. 2. The white tissues. Exposure to continuous illumination causes an enhancement of thylakoid swelling in the mesophyll chloroplasts of the white tissues of the japonica-2 maize mutant. In the bundle sheath plastids the effects of continuous illumination are striking and intriguing, because a regular honeycomb-like fret of membranes is formed from a provesicular body. No interpretation of this fret of membranes is, at present, possible.


Lymphocyte transformation in newborns, women and those who had used oral contraceptives. Lymphocyte transformation, utilizing the mitotic index parameter has been assessed in newborns' cord blood, adults and women who had been on oral contraceptives and their babies. The results show that the newborns' cord blood response is significantly higher to PHA when compared with the respective adults' response. No significant difference was observed between the women taking oral contraceptives and their respective controls.


A scanning electron microscopic study of trichomes of IAA-induced tumours in bean embryos (Phaseolus vulgaris L.). A scanning electron microscopic study of trichome morphology of bean embryo, growing in semi-solid synthetic medium, has been undertaken. The work shows some distinct differences in trichome morphology as well as variation in their development, due to the effect of indole-3-acetic acid (IAA).


Appearance and accumulation of the brain specific S 100 protein in the developing nervous systems of rat, rabbit and guinea pig. The appearance and accumulation of S 100 protein during postnatal development of rats and rabbit, and during pre- and postnatal development of guinea pig, was studied quantitatively by immunoelectrophoresis. High amounts of S 100 were found in newborn guinea pig brain. In rabbit and rat central nervous systems the content of S 100 rose linearily between the 1st and the 3rd and 4th weeks of postnatal life, respectively. From this later period to the adult animal there was a small increase in the amount of S 100 in rabbit and rat brain. The results of this study are compared with other studies on S 100 accumulation during postnatal development of rat, rabbit and guinea pig, using immunofluorescence and immunoelectron microscopic techniques.


Treatment of traumatic urethral lesions. From 1955 to 1975 the author has treated 705 patients with stricture of the urethra. He has elaborated his own procedure consisting of radical excision of the stricture and scar tissues with end-to-end anastomosis. In cases of long strictures, reconstruction of the urethra has been by the use of a scrotal flap.


The importance of the bladder washout technique in the management of a solitary calyceal stone. Renal infection is an indication for surgical treatment of a solitary calyceal stone. It must be proved however, that the infection is localised to the kidney before deciding on operation, as this may be very difficult and produce severe complications. Bladder washout seems to be an excellent technique to differentiate the localisation of the infection.


Management of ureteric obstruction in the solitary kidney by a segmental suspended ureteric prosthesis. Ureteric obstruction of a single kidney, secondary to an aorto-iliac bypass graft, was treated with a suspended segmentary ureteric prosthesis. No urinary stasis was observed during 1 year and the urodynamic implication are discussed.


Prevention of reflux by a ureteric intussusception valve: a preliminary study on dogs. In 10 dogs in anti-reflux procedure by intussusception of the ureter is examined. The surgical procedure is developed and the urinary flow and prevention of the anti-reflux valve are explored. The study shows that an invagination is technically possible and that reflux can be prevented in half the cases without significant obstruction of the urinary flow.


The influence of various drugs on artificial oedema of the rat's bladder. An experimental oedema of the bladder wall was produced by electric coagulation current in 130 rats. The amount of oedema was calculated by determination of the difference between the weight of the freshly excised wet bladder and that after drying to constant weight. Thus, oedema of a fairly constant and reproducible amount could be produced. The effect of three different drugs on such oedema was tested (Na-Aescinat, 6-methylprednisolone, alpha-Chymotrase). After the application of Na-Aescinat and 6-methylprednisolone no significant oedema could be measured after 48 h. Using alpha-Chymotrase, there was no measurable influence on the oedema.


Fibroelastosis of the posterior urethra associated with urinary, cardiac and digestive anomalies. A case of fibroelastosis of the posterior urethra associated with ectopic opening of the ureter of a solitary kidney in the urethra is described. Oesophageal atresia and anomalous origin of the left coronary artery were also observed. Management of fibroelastosis is pointed out.


A successfully treated case of condyloma acuminatum of the urethra and urinary bladder. Condyloma acuminatum very seldom affects the urinary bladder, but if it does conservative treatment has been considered of little value and cystourethrectomy will eventually be required. A case with urethrovesical condyloma is presented, where repeated transurethral resections cured the patient.


A transpubic approach to radical prostatectomy. A transpubic approach to radical prostatectomy is presented in a series of 15 cases. The main advantages and some typical complications of this approach are described.


[Structure of the cholinoreceptors].

[Structure of the cholinoreceptors]. The results of research works of a number of Soviet authors concerned with the structure of cholinoreceptors based on their response to cholinomimetics and cholinolytics are summarized. A brief review of foreign research involving separation of cholinoreceptors "in vitro" is given.


[Pharmacokinetic study of the neuroleptic, azabutyrone]. By using gas-chromatographic and spectrophotometric assays and adopting pharmacokinetic models of various types the pharmacokinetics of the neuroleptic azabutyron in the plasms, bile, gastric juice and urine of rabbits and rats was studied. The maximum level of azabutyron in the plasma (27  gamma/ml) was recorded 5 minutes after introduction of the drug. The amount of unchanged azabutyron passed with urine comprises 2--4 pc of the dose actually introduced, while the quantity of the agent excreted together with the bile and gastric juice was below 1 pc. The results thus obtained were subjected to electronic data processing according to the two-compartment pharmacokinetic model. It is presumed that in order to properly explain the pharmacokinetics of azabutyron some models of a greater complexity have to be used.


[Use of parameters of ionic permeability and mechanical activity for the rating of interaction of acetylcholine with the smooth muscle M-cholinoreceptors]. When employed in high concentrations (1-10(-5)-1-10(-3) g/ml) acetylcholine (ACC) causes an accrued uptake of Na22 and liberation of Rb86 (K) from smooth muscles of the rat's stomach. An increased release of Ca45 occurs in concentrations of 1-10(-7) g/ml and higher. The contractile reaction is recorded under the action of lower ACC concentrations (starting from 1-10(-9) g/ml). The observed changes in the ionic permeability and tonicity are due to the ACC action on the M-cholinoreceptors of the muscles. A comparison of emipirical relation between the effects and the concentration against the theoretical one showed that it is only the incorporation of Na22, liberation of Rb86 pand the contractile reaction in a depolarizing solution that adequately reflect the ACC interaction with cholinoreceptors of the smooth muscles in the rat's stomach.


[Effect of thyrocalcitonin on the pain sensitivity in animals]. With a single parenteral introduction of thyrocalcitonin (TCT) the algesthesia of soft tissues (tall skin, paw) is moderately increasing, while the algesic action of morphine and promedol against the background of TCT-gets weaker. And contrarywise algesthesia of hard tissues of the tooth becomes significantly lower under the effect of TCT and this continues for a long time.


[Study of interaction between contrast media and human serum albumin by the fluorescence method]. Structural changes in the human serumal albumin under the effect of roentgen-contrast media, viz. bilignost, triiotrast, endografine, iodamide, triombrine and cardiotrast, were investigated by the method of fluorescence. The complex-formation of albumin with triiodotrast, bilignost and endografine was shown to call forth a short-wave shift of the fluorescence peak by approximately 20 nm. The binding section for these substances is characterized by an association constant equalling 1.2-10(5) M-1. The authors presume that triiodotrast, bilignost and endografine cause changes in the microstructure of the aqueo-protein layer in the area of the tryptophan residue localization. As to triombine, iodamide and cardiotrast fluorescence failed to disclose their combination with the human serumal albumin.


[Antiarrhythmic activity of trimecaine in experimental arrhythmia and its effect on the heart conduction system]. The anesthetic trimecaine is shown to be capable of eliminating the flutter of atria in dogs simulated by an electric stimulation of the myocardium and atrial fibrillation in cats induced with aconitin and precludes ventrical fibrillation in rats arising due to intoxication with calcium chloride. Trimecaine noticeably mitigates the toxic effect of strophanthin. While depressing the automatism of the sinoatrial node the drug does not affect the conduction function.


[Effect of Soviet-made prostaglandin E1 on the aggregation of thrombocytes and erythrocytes]. The effect of prostaglandin E1 in a concentration of 0.1 gamma/ml on the aggregation and disaggregation of thrombocytes and erythrocytes in vitro was  studied. Prostaglandin E1 was found to materially reduce the  aggregation and to produce disaggregation of thrombocytes. A somewhat diminished aggregation of erythrocytes was also noted. Prostaglandin E1 failed to cause disaggregation of erythrocytes.


[Histological changes in the liver and lungs of rabbits after experimental hepatography with iophendylate emulsion]. Hepatography with a 75% iodophendylate emulsion was carried out in 21 rabbits to which the contrast medium had been introduced after laparotomy in a dose of 0.84-1.9 g/kg. The roentogenological and histological dynamics and the liver contrasting was studied in its continuity. In experimental hepatography the emulsification of iodophendylate does not cause any damaging effect on the liver and the lungs. In the mechanism of the emulsified iodophendylate elimination of prime importance are phagocytic cellular reactions on the part of lympho- and histocytic elements.


[Effect of mellaril on the liver lysosomes of rats with acute toxic hepatitis]. In 24 hours after a single administration of melleril (30 mg/kg) an elevated injuriousness of "heavy" and "light" liver lysosomes of rats subjected to "in vitro" treatment with a pH 5.0 solution at 37 degrees was recorded. Introduction of melleril to rats with toxic CCl4-induced hepatitis (CCl4 dosage of 0.15 ml per 100 g body weight) depresses free activity of acid phosphatase, this fall, however, failing to reach the corresponding values in intact animals. An elevated injuriousness of "heavy" and "light" lysosomas undergoing treatment at pH 5.0 and 37degrees, revealed in all variants of the tests, is related to activation of lysosomal phospholipases with subsequent liberation of surfactant lipids and volumetric changes of the particles.


[Characteristics of liver circulation and the outflow of bile and lymph in acute carbon tetrachloride poisoning]. In acute tests set up on 13 dogs the pressure and blood flow in the portal vein and hepatic artery, the pressure in the inferior vena cava along with lymph- and bile currents were registered following intraportal introduction of CCI4. Subject to determination were also the resistance of venous and arterial hepatic vessels and of the splachnic zone, as well as the summary blood flow. The rising pressure and diminished blood flow in the portal vein were found to be the result of the growing resistance of venous vessels in the liver. Falling pressure and reduced blood flow in the hepatic artery bore witness to the general toxic effect of CCl4 on the animal organism. Increased portal pressure and disturbed permeability of biological membranes was attended  by a greater lymph outflow. The inhibition of bile secretion came as a result of hypoxic and toxic damage of the liver.


[Antifibrotic properties of lidase in experimental berylliosis]. The effect of lidase with its inhalation against the background of a fibrotic process in the lungs was studied on a model of experimental berylliosis provoked in rats through intratracheal administration of 5 mg of Be (OH)2. The degree of fibrosis was assessed according to the dry residue in the lungs, content therein of oxyproline, hexosamines and hexuronic acids. Lidase was found not only to delay the development of the affections but also to lower the level of fibrosis existing prior to the treatment. The therapeutic effect of the compound continued for a comparatively long time.


[Effect of metaphos on the bile-forming function of the liver]. In experiments with albino male rats the effect of a single introduction into the stomach of methaphos in doses of DL50, 1/10 DL50, 1/20 DL50, 1/50 DL50 and also of a daily consumption of the compound for a month was studied. Metaphos was found to exert a manysided action on the cholopoietic function of the liver, viz. it increases the intensity of the bile secretion, inhibits the synthesis of primary bile acids and conjugation of cholic acid with taurine and glycine, stimulates the formation, conjugation and secretion of bilirubin.


An in vitro method for screening compounds for the effect on the rate of sickling of erythrocytes. Since no practical animal model is available for the evaluation of compounds in vivo, we have developed an in vitro model for determining the effect of compounds on the rate of sickling of erythrocytes in whole blood taken from patients with sickle cell anaemia. RMI 6792 (a phenethanol-diamine derivative), procaine, and L-glutamine were tested in this in vitro system. RMI 6792 was tested at various concentrations in whole blood. The data indicate that RMI 6792 decreased the rate of sickling at and above 60 microgram/ml. Procaine slightly decreased sickling rate at 100 microgram/ml. L-glutamine at 555 had no inhibitory effect. RMI 6792 and procaine had no effect on the oxygen dissociation curve. RMI 6792 affected the calcium flux of the erythrocytes and the calcium concentration in the erythrocytes.


Serum quinidine levels after chronic administration of four different quinidine formulations. The serum levels produced by four different quinidine formulations have been studied. The relative bioavailability of the formulations was demonstrated as were the mean peak serum levels and their timing in relation to dosage. From the data obtained, the biological half-lives were measured and the apparent volume of distribution and total body clearance were calculated for each formulation. The generic tablets of quinidine monosulphate from five different manufacturers were not significantly different from each other in any respect and produced the expected peak and trough serum level curves. The serum level curves resulting from administration of quinidine polygalacturonate (Cardioquin) were not significantly different from those resulting from the generic tablets, and this formulation may be regarded as therapeutically equivalent to the generic formulations. Both sustained-release formulations of quinidine bisulphate, Durettes and Kiditard (given at the same dosage) were shown to offer a means whereby, with simple twice-daily dosage, quinidine maintenance treatment may be continued with the confidence that the serum levels may be maintained throughout each 24-hour period without peaks into the toxic levels and troughs into the levels of no effect.


Systemic and coronary haemodynamic effects of the new hypotensive drug L 6150. The acute systemic and coronary haemodynamic effects of a new hypotensive drug L 6150 (3-hydrazino-6-[N,N-bis(2-hydroxyethyl)amino]pyridazine) have been studied in six patients with renal or essential hypertension. The drug, administered intravenously at a dose of 2-9 mg, caused a marked hypotension and increase of cardiac output in five cases. Though left ventricular work was reduced or unchanged after the drug, myocardial blood flow increased markedly, coronary resistance decreased and coronary A-V oxygen difference decreased by about 50% indicating coronary vasodilation. When compared with the available data on the effects of hydrazinophthalazine, L 6150 appeared to have qualitatively similar effects, but a somewhat greater direct coronary vasodilator effect.


A trial of Fortagesic and Paramol 118 in osteoarthritis. Fortagesic was compared with Paramol 118 in a fourteen day single-blind crossover study in patients with moderate pain due to osteoarthritis. Both preparations were shown to be similarly effective. More side-effects were seen with Paramol 118 than with Fortagesic and more patients preferred Fortagesic. These differences were not statistically significant, but it is concluded that Fortagesic is a useful effective analgesic for standard use in moderate pain.


Pharmacokinetics of amoxycillin according to administration schedule. Thirty subjects, free of any heart, kidney or liver disease, were given the same dosage of amoxycillin--100 mg/kg/24 hr. The first ten were given the drug in three equal doses at 8-hourly intervals, a further ten in four doses at 6-hourly intervals, and the last ten in eight doses at 3-hourly intervals. The final administration schedule resulted in much higher and more regular serum levels of amoxycillin than the other two, suitable for the treatment of severe infectious conditions, and has the advantage of avoiding prolonged antibiotic infusions which are a source of venous complications in the patient. The first results obtained clinically, which will be published in a further paper, seem favourable.


[Social psychological and sexological aspects of oral contraception (author's transl)]. An inventory is made on the hindrances to the acceptance of contraception in general and oral contraception in particular. They are grouped as hindrances related to the social and psychological significance of "making children", hindrances related to the social and psychological significance of "having children" hindrances, related to the method of oral contraception itself, to be divided in social hindrances, psychological hindrances and medical hindrances and finally hindrances related to the provision of the pill to the individual user. Each of these is amply discussed, the author expresses the hope that by identification of these hindrances, lessons may be learned for the future, when other methods of contreception become available.


Ovarian dysgerminoma, investigations on cell-and humoral-mediated immunologic reactions. A 17-year-old girl with repeated ovarian dysgerminoma is described. Postoperative immunologic investigations prior to irradiation showed a transient increased cell--an humoral--mediated immunologic responsiveness of the patient for approximately 2 1/2 months. After irradiation, a markedly defective response to PHA was observed which improved when reexamined 11 months later. The patient is well 22 months after the second operation.


[How to stimulate the placental function (author's transl)]. Imminent abortion, habitual abortion and threatened premature labor, all constitute difficult clinical problems. Those cases require on every occasion a diagnosis as acurate as possible, and unfortunately our present methods of biochemical determinations only represent a means to evaluate placental function. On those cases where a faulty placental function is detected thru the tests presently available, the authors recommend the utilization of a placentotropic substance, Gestanon, that is capable to stimulate and normalize the placental function, a is demostrated by the statistical results published in the international medical bibliography.


[Bovine milk progesterone concentration as a method of early diagnosis of pregnancy (author's transl)]. With the purpose of obtaining an early indicator of pregnancy in bovines, excretion levels of milk progesterone were determined by a simple radioimmunological method. In the pregnant group, progesterone concentration was significantly (p less than 0,001) greater than in non-pregnant ones. In some cases (20-25%) there was no difference in values, resulting a method efficiency of 75-80%. Milk must be processed a few hours after collection since a decrease in progesterone values is observed within a few days. Easy obtention of this biological matherial, without special technical requirements as well as the simple dosification method make this test a practical index for establishing early diagnosis of pregnancy in bovines.


[Post-pill amenorrhea and anovulation (author's transl)]. The numerous pregnancies that occur as early as the first cycle after the discontinuation of an oral contraceptive prove that in general ovulation reappears  immediately and that after a period of "rest" more or less prolonged the ovaries reasume their normal function very rapidly. However, in less than 1% of the cases the discontinuation of oral contrecption is followed by anovulation or even amenorrhea. The authors try to find an explanation to this over supression syndrome and review the different treatments that have been proposed in the bibliography, but keeping in mind that within a year 14% of the anovulations and 5% of the amenorrbeas will have disappeared spontaneously.


[Ultrastructure function and regulation of the oviduct of the Rana ridibunda (author's transl)]. An investigation has been carried out into the structure, ultrastructure, function and of the oviduct on the adult female of Rana ridibunda. The most important part of the oviduct comprises tubulary glands and a luminal epithelium which is composed of ciliated cells and vesicular cells. The discharge processes of secretory substances were studied. Injection of the mature females with estrogens and progesterone have show that progesterone was the most effective in provoking jelly release. It is probably that in Rana ridibunda the pituitary hormones act on the follicle cells of ripe oocytes, causing them to secrete a progesterone-like hormone which provodes the maturation of the oocytel and jelly release from the oviducal glands.


[A new technic for the correction of malunited distal radius fractures]. After malunited fractures of the distal radius the original anatomy of the wrist joint can be restored only by osteotomy of the radius and interposition of a cortico-cancellous bone block. This procedure has been shown to be much easier using a new bone plate for internal fixation. The plate is applied to the radius volarly through a radio-volar approach. The correcting angles can be adjusted easily. The stable osteosynthesis allows early postoperative motion. In cases of osteoarthritic changes the procedure may be combined with a denervation of the wrist joint (A. WILHELM).


[A rare form of median nerve injury during elbow dislocation]. The following is a report concerning a rare complication in the case of an elbow luxation. In the course of repositioning, the nervus medianus slipped into the joint cavity, causing total paralysis of the nerve medianus. Operative revision of the nerve and restoration through interfascular nerve transplantation. Results one half a year after the operation are already satisfactory. Since this complication does not sand in immediate connection with the accident, and in consequence representing a serious injury, besides being very rare--the literature produced only 6 cases--we see ourselves induced to further discuss the mechanism of injury and the problems of such an accident, and to point out the necessity of an early revision.


[Problems of treating the clubhand in dysmelia syndrome (thalidomide-embryopathy)]. In our follow-up examination of about 500 children suffering from thalidomide embryopathy we have studied the results of conservative and operative treatment of club hand. The results of treatment have not corresponded to the prognosis with regard to function. In our opinion the possibility of operative treatment should not be considered until growth of the ulna ceases.


[Familial occurence of Martin-Gruber anastomosis]. 1. A Case of MARTIN-GRUBER anastomosis detected intraoperatively is reported. 2. The two children of the above patient also showed a MARTIN-GRUBER anastomosis. The phenomenon was proved by EMG and local anaesthesia.


[Tenosynovitis nodosa].

[Tenosynovitis nodosa]. Nodular tenosynovitis occurs in a localized and in a diffuse form. The histologie finding are rather variable. According to electron microscopic studies nodular tenosynovitis originates in the synovial membrane. Mainly there are two types of cells to be found in the tumor: Type A, similar to macrophages - type B, similar to fibroblasts. The localized form of nodular tenosynovitis has a higher incidence in woman and occurs predominantly in the hand. The clinical symptoms are not characteristic and usually not very pronounced. More than one tumor in a single patient and also bone erosions caused by nodular tenosynovitis are rare occurrences. A case of a patient with multilocular occurrence of tenosynovitis is described here.


Thymic hormone activity and spontaneous autoimmunity in dwarf mice and their littermates. Serum thymic hormone activity (TA) was determined in hereditary hypopituitary dwarf mice (dw/dw) and their littermates (+/dw or +/+). It was found to be very low in the dwarf animals in comparison to their littermates. At 14 weeks of age, the dwarf littermates exhibited significant glomerular lesions characterized by deposits of IgG, IgG1, IgG2, IgA, IgM and C3, which were augmented by thymectomy of adult females. In contrast, hypopituitary dwarf mice had minimal glomerular deposits of immunoglobulins. Unlike these animals, their littermates showed antinuclear antibodies (ANA) and anti-deoxyribonucleic acid (DNA) antibodies in their serum. The present findings are discussed in relation to recent hypotheses on: (1) the role of the hypophysis in thymus-dependent immunological functions; and (2) the significance of T-cell deficiency in the development of autoimmunity.


Evidence for the genetic control of antibody affinity from breeding studies with inbred mouse strains producing high and low affinity antibody. The amount (Abt) and relative affinity (KR) of antibody produced in response to protein antigens injected in saline has been measured in the parents, F1 hybrids and backcross offspring of inbred mice which produce high and low KR antibody to these antigens. The results obtained support the view that antibody affinity is under polygenic control. Furthermore, strain related variation in Abt is independent of KR and the breeding experiments indicate that these two parameters are under independent genetic control.


Suppression of immune responsiveness by a submandibular salivary gland factor. Both tissue extracts prepared from submandibular salivary glands of male mice and epidermal growth factor isolated from these glands depressed the delayed type hypersensitivity response to 2,4-dinitro-1-fluorobenzene in mice. Extirpation of the submandibular salivary glands from male mice did not alter the delayed type hypersensitivity response. The role of salivary gland factors, particularly epidermal growth factor, in influencing the immune response has been discussed.


A phagocytosis-enhancing factor in human plasma. A phagocytosis-enhancing factor (PEF) with the capacity to stimulate the ingestion of sensitized sheep erythrocytes by human polymorphonuclear and mononuclear leucocytes has been isolated from human plasma by chromatography on DEAE-cellulose and filtration on Sephadex G-150 and Sephadex G-100. PEF is a protein of approximately 70,000 molecular weight which is susceptible to inactivation by heating at 60 degrees or by tryptic digestion. PEF promotes phagocytosis of erythrocytes sensitized with intact 7S antibody or bearing the C3b complement fragment, but not of unsensitized erythrocytes or erythrocytes sensitized with 19S antibody. The specificity of PEF interaction with target erythrocytes and the persistence of its stimulatory effect after the target cells are washed suggest that it promotes phagocytosis by an action on the erythrocytes.


A lipid chemotactic factor from anaerobic coryneform bacteria including Corynebacterium parvum with activity for macrophages and monocytes. A lipid with chemoattractant (chemotactic) activity for mouse and guinea-pig macrophages and for human blood monocytes is released by anaerobic coryneform bacteria (including Corynebacterium parvum). The active lipid is associated with fibrillar structures which lie on the outside of the bacterial cell and are released spontaneously during growth. The lipid can also be extracted easily by a number of methods. The fibrils are loosely associated with a capsule-like structure composed largely of polysaccharide. Purification of the active lipid was achieved by chloroform-methanol extraction of the whole organisms yielding a chloroform-soluble fraction attracting mononuclear phagocytes at concentrations around 10 microgram/ml. The infra-red spectrum of this material showed lipid but no peptide or sugar. Thin-layer chromatography yielded twelve spots of which three had chemoattractant properties. The most active of these gave staining reactions consistent with the presence of phospholipid, the other two probably contained free fatty acids and triglycerides. Thin-layer electrophoresis also yielded an active phosphorus-containing spot. Saturated fatty acids of chain lengths found in the anaerobic coryne forms had weak monocyte-attractant activity. As the active material was progressively purified, its activity as a monocyte attractant weakened.


Demonstration of thymus-independent immune system in Xenopus laevis. Response to polyvinylpyrrolidone. Larvae of Xenopus laevis were thymectomized at stage 45 (4 days old), and raised beyond metamorphosis. Thymectomized toads, when injected with polyvinylpyrrolidone (PVP), produced the same titres of antibodies as non-thymectomized animals, providing strong evidence that the thymus-independent immune response is present in this anuran. Antibodies were of exclusively high molecular weight, heat-stable, and 2-mercaptoethanol sensitive. After immunization, lymphoid accumulations were evident in the splenic white pulp of both thymectomized and non-thymectomized toads, but in the red pulp the accumulations were prominent only in non-thymectomized toads.


Quantitative and qualitative effects of cyclophosphamide administration on circulating polymorphonuclear leucocytes. The effect of cyclophosphamide (CY) on the absolute numbers and function of polymorphonuclear leucocytes (PMN) surviving in the circulation following either a single dose (100 mg/kg, i.p.) or daily administration (20 mg/kg, i.p., for 5 days) was studied in the guinea-pig. The quantitative effect of CY on peripheral blood leucocytes was assessed by measuring the absolute numbers of neutrophils, lymphocytes, and monocytes daily for 5 days following the initial injection of CY. The qualitative effects of CY on PMN function were determined by measuring the ability of these cells to function as killer cells. The two functional assays employed were the PMN-mediated PHA-induced cellular cytotoxicity and antibody-dependent cellular cytotoxicity (ADCC) assays against chicken erythrocyte targets. Both regimens of CY administration produced an equivalent degree of leukopenia 5 days after the initial injection with disproportionately severe neutropenia (less than 300 PMN/mm3). However, neither regimen of CY administration produced a significant decrease in cytotoxic effector function as measured through a wide range of effector to target cell ratios, PHA concentrations, and antiserum dilutions. These findings have clinical relevance in that they demonstrate the dichotomy between the quantitative and qualitative effects of (CY) on PMNs in that CY administration can dramatically decrease the absolute numbers of circulating polymorphonuclear leucocytes while leaving intact certain effector cell functional capabilities of those PMN surviving in the circulation during drug administration.


Reaction of an activated complex of guinea-pig complement components, C56, with unsensitized erythrocytes and with erythrocytes carrying C3b molecule. During the interaction of guinea-pig complement intermediate cells, EAC423, with guinea-pig C5 and C6, an activated complex of C5 and C6, C56, was demonstrated in the fluid phase of the reaction mixture. C56 also was eluted from EAC42356 which had been generated by the interaction of EAC423 with C5 and C6. Both preparations of C56 showed quite similar characteristics and were not distinguished from one another. Both were capable of reacting with unsensitized erythrocytes (E) in the presence of C7 to form EC567. Further, they were able to react with EAC43 in the absence of C7 to form EAC43568 but did react with EAC43 pretreated with C3b inactivator, dithiothreitol or N-bromosuccinimide. These results indicate that guinea-pig C56 generated on EAC423 has a tendency to dissociate into the fluid phase. Nevertheless, the dissociated C56 can bind again to intact C3b molecule on the cells. The ability of cell-bound C3b to combine with C56 may lead to localization of C56 to the cell membrane carrying C3b, resulting in acceleration of attachment of C567 to the membrane. This assumption could be supported by the finding that the replacement of E by EAC43 increased the susceptibility of the cells to lytic action of complement induced by cobra venom factor. Thus, a new function of cell-bound C3b as localizing C56 to the membrane of sensitized cells was indicated.


Local immunity in the respiratory tract of the chicken. I. Transudation of circulating antibody in normal and virus-infected birds. Three-week-old chickens were given sheep erythrocytes or bovine serum albumin intravenously. Seven days later their tears and saliva possessed low levels of antibody to those antigens. Concurrent infection with lentogenic Newcastle disease virus (NDV) caused a significant increase in transuded antibody in those fluids. In chickens with circulating antibody to NDV, induced by parenterally administered inactivated vaccine, respiratory infection with heterologous infectious bronchitis virus resulted in limited transudation of anti-NDV. In contrast, the tears, saliva and tracheal fluid of non-vaccinated chickens undergoing primary infection with NDV acquired considerable levels of specific anti-NDV. The difference between the two groups is attributed to locally synthesized antibody.


Specific suppression of delayed hypersensitivity skin reactions to collagen in guinea-pigs after immunization with collagen and Freund's incomplete adjuvant. Partial suppression of cutaneous delayed hypersensitivity reactions to collagen in guinea-pigs was induced by pre-immunization with collagen and FIA. This suppression is specific since: (a) pretreatment with OA and FIA or FIA alone did not cause suppression of skin reactions to collagen; (b) suppression was observed only if the collagen used for pretreatment was from the same species as that employed for sensitization for delayed hypersensitivity reactions; and (c) animals with depressed skin reactivity to collagen reacted normally to PPD. The suppression is not mediated by inducible, circulating antibodies to collagen since: (a) antibody titres measured by passive haemagglutination did not correlate with the degree of suppression; (b) suppression was observed with collagen in random coil conformation which sensitizes guinea-pigs for delayed hypersensitivity skin reaction but does not induce antibodies to denatured collagen; (c) best suppression was obtained if the animals were pretreated with collagen and FIA 3 days before the sensitizing injection; and (d) passively transferred antibody from animals with suppressed skin reactivity did not suppress skin reactivity of animals made hypersensitive to collagen by injection of collagen and FCA.


Intracellular modifications induced in mouse submaxillary glands by antibodies directed against saliva. The purpose of this paper is to investigate whether an antibody directed against the exocrine secretion, saliva, is able to induce a modification in vivo within the specific cells of the submaxillary glands which synthesize this secretion. The salivary antigens synthesized within the glandular acinar cells are secreted into their respective lumen and are not present in the general circulation or in the intercellular spaces of the gland. When heterologous anti-saliva antibodies are injected into mice, they induce marked lesions within the acinar cells of the submaxillary glands. When different antibodies recognizing different salivary components are injected, they all induce similar modifications; it is not yet possible to distinguish whether different cells are involved in the synthesis of the different salivary components. The lesions described seem specific since other organs studied, such as the pancreas and the stomach, are unaffected. The different possible mechanisms of this cytotoxic effect are discussed.


Further characterization of IgA in chicken serum and secretions with evidence of a possible analogue of mammalian secretory component. Immunochemical studies of the intestinal secretory immune system of the chicken have led to further characterization of IgA in bile, intestinal contents and serum. A component was detected in late Sephadex G-200 fractions of caecal and intestinal contents which showed partial identity with bile, intestinal and a high molecular weight fraction of serum IgA. This component showed similar sedimentation characteristics to bovine serum albumin in sucrose density gradients, a fast electrophoretic mobility on polyacrylamide gel and is a possible analogue of mammalian secretory component (SC). Fractionation of serum from birds affected with infectious synovitis revealed two moleculare classes of IgA. Comparative double diffusion studies produced a reaction of complete identity between bile IgA and high molecular weight serum IgA (15S) and partial identity with low molecular weight serum IgA (7S), suggesting a lack of an SC determinant on the latter. A spur of partial identity between 15S and 7S serum IgA was also observed. Although no direct structural homology with mammalian or human IgA could be demonstrated by immunological cross-reactivity, the similarities of molecular characteristics, particularly emphasized by the presence of a secretory component, favour a functional analogy between the secretory immune system of the fowl and mammalian species.


Immune response in the garter snake (Thamnophis ordinoides). Garter snakes (Thamnophis ordinoides) were immunized with hen egg albumin, human gamma-globulin and Keyhole limpet haemocyanin in Freund's adjuvant. Antibody was consistently detected by radioimmunoelectrophoresis and in three different gamma- and beta-globulin precipitin lines called IgM (approximately or equal to 20S), Ig-1 (approximately or equal to 9S) and Ig-2 (approximately or equal to 8-5S). Early antibody (day 31 after immunization) was frequently Ig-M whereas Ig-2 and especially Ig-1 were detectable for the longest duration (992 days). After immunization with antigen in Freund's adjuvant, Ig-1 serum concentration showed the greatest increase, from almost undetectable levels to the most prominent immunoglobulin in immune serum.


Studies on the role of suppressor cells in specific unresponsiveness to DNCB. Lymph nodes--and to a lesser extent spleen cells--from guinea-pigs tolerant to DNCB contact sensitivity, when injected into normal syngeneic guinea-pigs, decrease the ability of the recipients to become sensitized to contact with DNCB. The difference between the complete tolerance transferred by parabiosis with tolerant partners and the partial tolerance induced by transfer of tolerant cells can be explained by the different numbers of cells homing in the recipients. The tolergen does not play any role in the transfer of tolerance.


Specialized structure and metabolic activities of high endothelial venules in rat lymphatic tissues. Microscopic, histochemical and ultrastructural techniques were used to define characteristics of high endothelial venules (HEV) in rat lymphatic tissues. This endothelium contained acetyl esterase and acid hydrolase activities which were not altered by lymphocyte depletion. No immunoglobulins were detected on luminal surfaces of HEV by fluorescent antibody staining. Only minor structural differences were seen between HEV within lymph nodes and Peyer's patches. At both sites, high endothelial cells were linked together by macular junctional complexes and interlocking basal foot processes. Endothelial cell cytoplasm moulded about surfaces of lymphocytes migrating through the venular wall, and flocculant deposits of basement membrane formed over lymphocytes penetrating the basal lamina. The endothelium was ensheathed by three to five layers of overlapping reticular cell plates and connective tissue. Each plate was linked to the reticular meshwork of the node by collagen bundles and anchoring filaments which inserted into the plate's external limiting membrane. This permitted individual paltes to separate or approximate each other as tissue and intravascular pressure varied, and lymphocytes moved across the sheath by insinuating themselves into gaps between overlapping plates. This composite structure of the HEV wall appeared to facilitate lymphocyte entry into the node and minimized vascular leakge.


The nature of the local immune system of the bovine small intestine. In an examination of the immunoglobulins of the small intestine of calve, IgG1 was the major immunoglobulin in the secretions, and IgA was present in smaller amounts. Immunohistochemical studies showed few IgA containing plasma cells but many cells that stained with anti-IgG antiserum. Histological evidence of transport of IgG1 across the crypt epithelial cell was found. Studies with 125I-labelled IgG1 indicated that a significant proportion of IgG1 in the intestine originated in serum.


The circadian variations of the rates of excretion of urinary electrolytes and of deep body temperature. 53 apparently healthy subjects were studied for control spans of between 60 and 184 hours while they followed a regular pattern of meals, ambulance and sleep before experiments on altered time schedules. Urine was collected and deep body temperature was measured every 2 hours while they were awake, and in some rectal temperature was also recorded during sleep. Urine was analysed for sodium, potassium, chloride, phosphate and creatinine and in 26 subjects also for calcium and uric acid. The rhythms of temperature, urine flow, and rate of excretion of each urinary solute, are presented as consinors. The two rhythms most closely linked were of excretion of chloride and sodium; the choloride acrophase led the sodium acrophase by one a half hours.


Two biological rhythms of perception distinguishing between intact and relatively damaged brain function in man. A simple test of perception, the Critical Flicker Fusion threshold (CFF) was given successively for 20 mins. to 69 healthy subjects aged 7-63 and to 53 comparable neuropsychiatric patients. The latter could be divided into a functionally sick group and a group with brain damage. Auto-correlation analysis revealed significant sine-wave cycles of amplitude and ultradian frequency for the CFF mean scores and the CFF "Scatter* scores. Both cycles of recurrence showed frequencies which distinguished significantly between the total healthy subjects and the patients. Further analysis showed no difference between controls and emotionally sick patients (i.e. those with sociopathy, schizophrenia and manic depressive psychosis) but there was a highly significant difference between these three groups and patients with relatively damaged C.N.S. (i.e. those with mental deficiency, organic brain syndrome and organic dementia). Even among the control group a progressive increase in frequency of these perceptual cycles occurred with advancing age. These CFF results point to the existence of a cycle of perceptual acuity and another of vigilance in the organism. Both appear to relate to the neural integrity of the C.N.S.


A chronobiological investigation of fitness for work in women on a 220 cal 24 h protein diet. In a group of nine healthy but obese women a set of tests were used to estimate several physiological and psycho-physiological variables: self-rating of hunger, mood and physical vigor, self-recording of grip-strength, muscular fatigue test, eye-hand skill, tempo, etc. Measurements were performed every 6-hours, at fixed times, during 24-hours, once a week, before (non-restricted spontaneous food intake) and for three weeks on a 220 cal. special protein diet (calcium caseinate: 55g, associated with potassium salts, water and vitamins). Findings relating to the metabolic and hormonal variables while on this diet have already been reported: (a) The diet does not alter the circadian acrophase (peak) of the variables and (b) two distinct metabolic steps occur: first a peak of gluconeogenesis and second, a phase when ketone bodies and fatty acids are used almost exclusively as source of fuel. The present findings show that so long as nitrogen remains in balance this restricted diet (a) does not change the grip strength and the muscular fatigue test; (b) does not alter the mood, the physical vigor etc. and (c) induces a statistically significant fall in "hunger" (self-rating method). We conclude that this low protein diet is compatible with non-strenuous work.


The chronomutagenic effect of deuterium oxide on the period and entrainment of a biological rhythm. II. The reestablishment of lost entrainment by artificial LD cycles. Mice caused to ingest 25% or 30% deuterium oxide could no longer be entrained to a 12:12 light-dark cycle. Reentrainment could be established by artificially lengthening the light-dark cycle from a natural 24-hours one, to LD 13:13 or 14:14. It is noted that the relationship between biological rhythms and the ambient LD cycle is strikingly similar to that between coupled unidirectional physical oscillators. It is concluded that while the similarity between the responses of a great many organismic rhythms to deuterium oxide administration suggests that the biological clocks of all organisms are very similar, if not the same, a substance with more specificity to key biological processes than D2O will be required to eventually elucidate the escapement mechanism of the enigmatic living clock.


Acetabulaira mediterranea: circadian rhythms of photosynthesis and associated changes in molecular structure of the thylakoid membranes. Acetabularia mediterranea algae, grown in three different light-dark regimes, were frozen in liquid nitrogen at c.t.(1) 0 and c.t. 6 and a record made of 77 degrees K fluorescence emission spectra of their chloroplasts. Algae grown under LD cycles exhibited a clear circadian rhythm of oxygen production. The low temperature fluorescence emission spectrum at c.t.0 was different from that at c.t.6 and this difference was increased by submitting the algae to successive "freeze-thaw" treatment. Similar results were obtained in DD, and the photosynthesis rhythm remained fully expressed. Algae grown in LL, where no rhythm of photosynthesis could be detected in the samples because there is a great individual variability in period lenght under these conditions, exhibited a similar difference in their low temperature flourescence emission spectra between c.t.0 and c.t.6. We conclude that the circadian rhythm in low-temperature fluorescence emission of the chloroplasts in Acetabularia is related to the circadian rhythm in photosynthesis.


Irradiation-induced changes in circadian DDT-susceptibility and metabolic rhythms in the housefly. The diel pattern of susceptibility to DDT of houseflies, reared under LD 14 : 10, was shown to be affected by 10 Krads of gamma radiation administered at the pupal stage. An additional peak in susceptibility of the flies to DDT corresponded to the time of irradiation of the pupae. Irradiated flies had higher general LD50 values than untreated flies. Flies reared in darkness demonstrated no discernable group rhythm apart from an altered susceptibility at the pupal irradiation time. Respiration rates of irradiated flies were slightly lower than the control flies and did not change at the time of irradiation. Groups of flies reared in darkness have no significant respiration rhythm.


A self-assessment questionnaire to determine morningness-eveningness in human circadian rhythms. An English language self-assessment Morningness-Eveningness questionnaire is presented and evaluated against individual differences in the circadian vatiation of oral temperature. 48 subjects falling into Morning, Evening and Intermediate type categories regularly took their temperature. Circadian peak time were identified from the smoothed temperature curves of each subject. Results showed that Morning types and a significantly earlier peak time than Evening types and tended to have a higher daytime temperature and lower post peak temperature. The Intermediate type had temperatures between those of the other groups. Although no significant differences in sleep lengths were found between the three types, Morning types retired and arose significantly earlier than Evening types. Whilst these time significatly correlated with peak time, the questionnaire showed a higher peak time correlation. Although sleep habits are an important déterminant of peak time there are other contibutory factors, and these appear to be partly covered by the questionnaire. Although the questionnaire appears to be valid, further evaluation using a wider subject population is required.


[A rare form of cancer of the common bile duct: papillary epithelioma. Report of 4 cases]. Papillary carcinoma is rare as it represents only 5 p. cent of carcinomas of the common bile duct. The authors report 4 cases and emphasize the special characteristics of this carcinoma. The long prodromal phase, dominated by attacks of cholangitis and the incomplete appearance of the jaundice when fully constituted. It affects mainly the common bile duct and has, on microscopy, a villous appearance. Its papillary structure consists of fine coalescent fringes producing a typical glandular appearance. Finally, it evolves very little, therefore there is a good hope of survival even after palliative treatment.


[Fractures of the lower extremity of the femur. Therapeutic indications and results of a series of 86 fractures]. The authors report 76 recent fractures of the lower femoral end. The types of fractures, the treatment and the results are analyzed. Kuntscher's close wound intramedullary nailing with reaming should be employed for transversal supra-condylar fractures and classical osteosynthesis for other cases.


Culture and the development of vision.

Culture and the development of vision. Given Chinese ethnographic data, a model of a style of visual performance was predicted. The prediction was that the style would be "inwardizing". Inwardizing was related to individual styles of social behavior as observed in optometric offices. A complete 21-point optometric examination was performed on Chinese subjects and the results were compared to a control group of American subjects. Throughout the optometric examination the Chinese subjects displayed the predicted pattern of inwardizing visual performance. That physiologically measurable patterns of visual performance could be predicted on the basis of ethnographic data gives experimental support to the anthropological assumption of reciprocity between biology and culture. The discovery of a culturally determined pattern of visual performance beyond the work already done with various isolated refractive conditions and the observed relationship of this to patterns of social behavior pose interesting tasks for anthropology, optometry and other professions. First, there is need for further investigation of 1) the role of culture in determining visual performance and 2) the relationship of visual performance patterns to social behavior, beyond that which has already been done, for example, with myopia and hyperopia. Then there is need to incorporate these new areas of knowledge into all fields dealing with human behavior.


[Palilloma and papillary tumor of the gallbladder]. Frequently confused with adenomas or pseudopolyps of the gall bladder, papillomas are rare tumours defined as vegetations covered with an epithelium of gall bladder type in the centre of which is a thin connective tissue axis. They are usually recognised on lyon histological examination of the operative specimen. The uncertainty concerning their course makes them a potentially malignant lesion as shown by the two cases reported here including one personal case concerning a carcinoma situated close to a benign papillomatous lesion. The frequent coexistence with lithiasis which may induce their malignant transformation reinforces the indications for surgery for gall stones.


Systemic arterial blood pressure and its relevance to optometric practice. The measurement of systemic blood pressure is a very significant detection test that can provide a great deal of information for the optometrist. The blood pressure readings can be critical in aiding the practitioner's evaluation of both the patient's visual health and related systemic condition and in indicating the possible need for referral. This paper presents a general survey of the benefits blood pressure measurements can provide in the optometric practice.


[Barium granuloma of the rectum].

[Barium granuloma of the rectum]. The authors report a case of barium granuloma of the rectum. This complication of barium enema is exceptional. During enema, the cannula may ulcerate the mucosa which is blown up secondarily by the barium. Clinically, the granuloma presents like a hard polyp which is suggestive in certain cases of a malignant tumour. The precise diagnosis is made by careful examination of the abdominal films before administration of barium taking in the whole of the pelvis.


[Rupture of the subclavian artery. Apropos of a case]. The authors report a typical case of rupture of the sub-clavian artery in closed trauma of the shoulder and review the world literature. The pathology of this lesion is described and its mechanism studied, it seems to be due to an acute encounter between the artery and the scalenus anterior.


[Ureter and Wertheim's operation].

[Ureter and Wertheim's operation]. The surgery for carcinoma of the uterine cervix combined with radiotherapy, as carried out at present, includes a definite risk for the urinary tract. Not so much the immediate functional disorders, which always recede, except in rare cases of fibrous retracted bladder, but the lesions which occur in several varieties lead one to re-implant immediately the ureter. Fistulae and early stenosis (16 out of 1,500) should be treated in the same way. These late complications, such as obstruction (12 out of 1,500) raise de problem of their etiology. Usually they are due to recurrent carcinoma (9 out of 12), rarely due to pelvic fibrosis (3 out of 12). It is rare then for re-implantation to be possible. There remains either nephrectomy which is the simplest solution, or an urinary by-pass operation either using an ileal bladder or as a palliative measure, using a silastic prosthesis. Finally, the risk of advanced carconoma is without remedy, abstention from surgery may then be justified but one should, as far as possible, attempt to prevent these complications both from the surgical technical point of view and from the point of view of irradiation dosage.


Clinical manifestations of visual information processing: part I. This paper discusses three aspects of visual information processing and their significance to clinical optometric practice. It analyzes the factors involved in central-peripheral organization, pictorial-linguistic interaction and total versus partial utilization os sensorimotor information as they relate to perceptual style and visual function. It emphasizes these factors as possibly being the commonalities behind many of the divergent yet generally successful theoretical frameworks and techniques utilized in optometric diagnosis and therapy.


Impact testing of ophthalmic lenses: stress distribution and the "search" theory. Elementary concepts in fracture mechanics, stress analysis, and impact are discussed as they pertain to fracture of lenses in drop ball testing. Quasi-static estimates for stress distributions during impact are obtained. These profiles show a sharp peak in the tensile stress under the impact point. Identical groups of lenses were impact tested in two different modes: 1) 25 impacts at a single spot on the lens, 2) 1 impact at each of 25 different spots. Much more breakage was observed in the latter group. Both the experimental and analytical considerations lend support to the "search" theory of drop ball testing--fracture occurs when the ball strikes a weak spot (where there exists a sufficiently severe flaw) on the lens. Lenses which pass an impact test may well have dangerous flaws away from the impact point.


Should there be a restriction of optometric treatment? The restriction of optometric services in third party payment programs is of considerable concern to the profession. Limitations placed on the type and amount of lens prescriptions which can be provided is not in the best interest of the patient. Those outside the profession of optometry must be made aware of the significance of ophthalmic lens prescriptions in terms of total visual performance rather than mere correction of "significant" refractive errors.


[Transit-slowing anastomosis by 180 degree axial rotation of the upper intestinal segment after massive resection of the small intestine. Preliminary note on an experimental study in the adult dog]. After massive resection of 85 p. cent of the small intestine in the dog, there occurs diarrhoea and malabsorption. These consequences may be palliated by an oblique end-to-end anastomosis with 180 rotation on the intestinal axis of the jejunal sugment above in relation to the ileal segment below. The authors noted slowing of the transit in the 10 operated dogs. The experimental conditions and the results obtained suggest that the technic may be applicable in man.


Research in orthokeratology. Part III: results and observations. The effects of contact lenses, fitted by defined conventional and orthokeratological techniques, are given and compared to a group of non-contact lens wearers. Data presented include changes in corneal curvature, topography, refraction and astigmatism. General trends and variability of data are discussed in conjunction with pertinent clinical observations for the three test groups.


The interpretation of systemic arterial blood pressure. Part III: hypertension retinopathy. Systemic blood pressure is a vital sign and fundamental variable and as such the areas of its involvement in all individuals are indeed multifaceted. The purpose of this paper is to discuss the interpretation of systemic blood pressure readings as they specifically relate to a detailed analysis of hypertensive retinopathy.


Tear breakup time and the Schirmer tear test. An analysis of tear film breakup time (BUT) and the Schirmer tear test in 40 optometric students demonstrated that BUT cannot be used as a tear quantity indicator.


A descriptive epidemiological study of optometry services in the Birmingham Veterans Administration Hospital. The Optometry Service in the Birmingham Veterans Administration Hospital has been delivering comprehensive vision care to outpatients and inpatients for almost 3 years. The veteran population receiving care and the optometric services provi4ed are described.


The rural optometrist: who and why?

The rural optometrist: who and why? A first step toward the goal of better optometric manpower distribution is the identification of variables associated with the optometrist's choice of practice location. This study focuses on the optometrist who chooses a rural practice location and on ways in which the rural optometrist differs from the non-rural optometrist. One hundred sixty optometrists practicing in rural locations in ten states answered questions about their background, mode of optometric practice, attitude toward the environment, interests, and personality traits. Rural optometrists were found to differ from other optometrists in their rural origin, their strong liking for the people and life style of the rural community, and their interest in outdoor pursuits. Community recognition as a health professional, rapid practice growth, professional independence, and the opportunity for community leadership were seen by the rural optometrist as major advantages of rural optometric practice.


Health planning and resources development: an opportunity for optometry. The American Optometric Association views the goal of the National Health Planning and Resources Development Act of 1974, as the promotion of physical, emotional and environmental health for all. The National Health Planning and Resources Development Act will work towards this goal by identifying problems, preparing reports and studies and developing recommendations for implementation directed toward solving the problems. These functions will be carried out in collaboration with private and governmental individuals and organizations concerned with health care services. Only through this coordinated effort will success be achieved.


Optometry and NHI--where are we going.

Optometry and NHI--where are we going. The author reviews and estimates the overall trend toward some form of national health entitlement against a background of optometry's position on this issue. He summarizes how optometric services are included in most major NHI bills, and explains the relationship to NHI of the National Health Planning and Resources Development Act of 1974. Stressed are the needs for optometry to more accurately describe its own role and to continue active interest in the NHI issue.


Optometry and the military.

Optometry and the military. This article comments on several of the major issues facing military optometry which were aired earlier this year at the AOA Federal Service Optometry Seminar. Among these issues, all of which influence recruitment and retention of optometry officers, are constructive credit, bonus and incentive pay, promotion policies, and optimum scope of care. Dr. Turner emphasizes that solutions to these problem areas depend not only upon AOA actions but also personal efforts and professionalism of military OD's.


Professional participation in HMO's.

Professional participation in HMO's. As HMO's spring up throughout the country, a conflict between state and federal laws becomes evident. The traditional standards of practice by which the solo practitioner abides may no longer be enforceable against those physicians, dentists, and optometrists working in HMO's.


Comprehensive vision care: a model.

Comprehensive vision care: a model. This paper suggests a model for the practice of vision care in which the optometrist and ophthalmologist are each used to maximum advantage. It is demonstrated how the present antagonism between the two fields can be removed with a resultant enhancement of the practice of both optometry and ophthalmology. The natural outcome of the new relationship will be better vision care for the public.


Rationalizing the delivery of eye care--part I. The often proclaimed health care crisis has led to a variety of efforts to reorganize and rationalize the existing health care system. One such effort has been to develop a conceptual model for the delivery of personal health services in which the region is the fundamental planning unit and levels of care form its organizational framework. Three levels of care are defined--primary, secondary, and tertiary--each level being based on a combination of the frequency with which a service is needed and its professional and technical sophistication. The regionalization of health services would serve to "achieve a balance between the public's request for decentralized resources and the centralization required so that they may be used efficiently."


Optometric manpower development and utilization: a look toward the future. This paper attempts to discuss in detail the rise in demand for optometric services and the manpower problems of the future. A proposal for the rational future development of optometric manpower has been made. Hopefully optometry will be able to consider its future development and scope of practice, and the types of changes to be made as the profession prepares for its increasing role in providing quality vision care to all Americans.


A retrospective analysis of the systolic BP/IOP ratio in glaucoma screening. It has been postulated that the BP/IOP ratio is more predictive of glaucoma than is tonometry alone. An analysis of clinical data from 128 glaucoma patients and 115 normals yields a sensitivity of 80%. Specificity of 86% and relative risk ratio of 22 for this screening test. Evidence of the existence of a dose-response relationship is also found.


Optometry and genetic counseling: the first lines of defense against blindness. Genetic diseases may be present at birth or may be manifested later in life. The best preventative measure for either type is early referral of the affected family for genetic counseling. Since several genetic disorders include ocular complications either primarily or secondarily, optometrists are likely to encounter some of these disorders. If the family is unaware of the hereditary pattern, they should be referred to a geneticist to be advised of the risk rates for having future children affected. A partial listing of the counseling centers throughout the United States is provided.


Suggested criteria for vision classification on the AAMD adaptive behavior scale. The AAMD Adaptive Behavior Scale for Children and Adults is an instrument designed to assess the degree to which an individual has adapted to his environment. While one item of the scale refers to vision, no criteria have been established to indicate degree of vision impairment on such a scale. Such criteria are suggested including a supplement which describes other visual functions. A method of assessing visual acuity in non-verbal or uncooperative patients is given.


Vision anomalies of Indian school children: the Lame Deer study. The occurrence of vision anomalies among American Indian school children is measured for an entire school population, according to grade (pre-kindergarten through 12). The anomalies are identified, classified in three major groups (myopes, astigmats, all other), and each group is compared to Caucasian school children. Some conclusions and recommendations are made concerning the vision care needs of Amerind school children.


Rationalizing the delivery of eye care--part II. The provision of eye care within the previously described regional model requires that resources be distributed in a rational manner. In a levels of care approach those eye care services that would meet most of the people's needs most of the time would be provided at the primary level, on an ambulatory basis. At the secondary level those more specialized services such as glaucoma surgery would be provided, usually at the community hospital setting. At the tertiary level those superspecialized services requiring the resources of a major medical center would be provided. Such a system of eye care would require changes in the role and responsibility of both optometrists and ophthalmologists.


A rational approach to license drivers using bioptic telescopes. A great deal of interest and controversy has arisen about the ability of persons with reduced vision driving with bioptic telescopes. This article is the author's approach to the public. The author has been fitting drivers with bioptic lenses for approximately ten years.