Influence of flow velocity and cell concentration on dynamic adhesion of erythrocytes to glass surface. Comparative studies were carried out on dynamic adhesion of 51Cr-labelled erythrocytes to the surface of glass beads in the presence of serum in the medium (50 microng of protein/ml) and in protein-free medium. The influence of cell concentration (within the range 4 X 10(5) to 8 X 10(6)/ml) and of cellular flow velocity (within the range 1.5-0.4 cm/min) on the value of adhesion was investigated. It was found that when serum was present in the medium, the decisive influence on erythrocyte adhesion was exerted by the velocity with which the cells pass though the glass bead layer. Cell concentration under these conditions has only a very slight effect. When the medium does not contain serum, erythrocyte adhesion to the bead layer seems to depend on both cell concentration and flow velocity. Preliminary data were obtained concerning the release of 51Cr from the bead layer after erythrocyte adhesion.


Effects of withdrawal of a mitogenic stimulus on progression of fibroblasts into S phase: differences between serum and purified multiplication-stimulating activity. Multiplication-stimulating activity (MSA) for chicken embryo fibroblasts was purified from serum-free medium conditioned by the growth of a rat liver cell line. A comparison between calf serum and purified MSA was made regarding the regulation of the fibroblast cell cycle. Addition of serum or MSA to stationary, quiescent cells stimulates them to enter the DNA synthetic phase after a characteristic lag period. Exposure to serum for shorter periods of time will irreverisbly commit cells to continue through the cell cycle and initiate DNA replication in the absence of serum. In contrast, the withdrawal of purified MSA from the medium results in an abrupt halt in the progression of cells towards S phase. The results of labeled thymidine incorporation and autoradiographic experiments clearly indicate that the point at which cells become irreversibly committed to enter the DNA synthetic period is at or near the G1-S boundary. The abrupt decay of the stimulation upon withdrawal of purified MSA provides a unique opportunity to investigate the biochemistry of this discrete phase of the cell cycle.


Milk and tissue lipid composition after feeding cows protected polyunsaturated fat for two years. The long-term effects of feeding Holstein cows plant lipids protected from microbial hydrogenation in the rumen were studied. Of particular interest were cow health and changes in fatty acid and cholesterol concentrations of milk and meat. Safflower oil-casein or safflower oil-casein treated with formaldehyde to impede microbial attack were fed to two groups of three cows as 10% of the concentrate ration for two lactations. Production of milk fat of cows fed the protected concentrate increased significantly. Linoleic acid of milk fat was twice normal, providing a polyunsaturated milk. Cholesterol of milk or meat did not increase even though cholesterol of blood plasma was higher in both groups fed safflower oil than in control cows. Cardiovascular systems showed no marked abnormalities and no differences that could be due to treatment. All cows maintained normal health and milk production throughout the experiment.


Relationships between radioimmunoassays of alpha lactalbumin and prolactin in bovine skim milk. A radioimmunoassay developed for alpha-lactalbumin was sensitive between 5 and 140 ng alpha lactalbumin. Addition of increasing volumes of milk to assay tubes progressively decreased binding of iodine-125-labeled alpha-lactalbumin to the antisera in a manner which paralleled the binding curve generated by increasing concentrations of standard alpha-lactalbumin. The addition of 10, 20, 30, or 40 ng of alpha-lactalbumin to diluted milk samples gave 90, 100, 105, and 102% recoveries. Alpha-lactalbumin antisera did not crossreact with 1000 ng of bovine casein, blood serum albumin, beta-lactoglobulin, prolactin, or growth hormone. Milk from each of approximately 100 Holstein Friesian cows at different stages of lactation was sampled monthly for 12 mo. Concentrations of alpha-lactalbumin (1.63 mg/ml) and prolactin (24.9 ng/ml) in samples of skim milk collected in the 1st mo of lactation were greater than those in the remaining months of lactation. Monthly concentrations of alpha-lactalbumin and prolactin in skim milk did not change significantly during seasons of the year. The correlation between concentrations of prolactin and alpha-lactalbumin pooled within subclasses of month of lactation within month of year was .08 for 1125 pairs.


Effect of age on the saturated lecithin contents of human and rat lung tissues. Changes with age in the lecithin (especially saturated lecithin) content of human and rat lung and in lecithin synthetic activities in rat lung were examined to obtain information on lecithin metabolism in the lung in old age. In lung tissues of humans and rats, the content of saturated lecithin as a percentage of total lecithin was fairly constant (about 35%) throughout adult and old age; The percentage content in bronchial wash was 55--60% in both young and old rats. The radioactivities incorporated into lung tissue per milligram total and saturated lecithin after intravenous administration of palmitic acid3-H were similar in young and old rats. These results suggest that lung tissue contains a rather constant amount of saturated lecithin throughout adult and old age and that lecithin synthesis in the lung is still active in old age.


Aging and ovarian steroidogenesis in the rat. Ovaries obtained from rats of 4 and 18 mo of age were incubated in vitro with 3H-pregnenolone as the added precursor. Incorporation of radioactivity into progesterone (P), 20alpha-dihydroprogesterone (20alpha-P), 17alpha-hydroxyprogesterone (17alpha-OHP), androstenedione, and testosterone were studied by serial paper and thin-layer chromatography. Significantly more P, 17alpha-OHP and androgen was produced by the older ovaries. Addition of hCG (100 I.U.) to the incubate increased 20alpha-P production in the ovaries of both age groups, depressed 17alpha-OHP and androgen formation in the ovaries of older rats, and had no effect on progesterone synthesis in either age group. Serum progesterone in 4-mo-old rats was 13.5 +/- 1.91 ng/ml at estrus and 3.69 +/- 0.9 ng/ml at diestrus, whereas serum progesterone in 18-mo-old rats was 0.85 +/- 0.15 ng/ml at constant estrus and 1.23 +/- 0.54 ng/ml at constant diestrus. Treatment with hCG for 20 days increased serum progesterone to 29.6 ng/ml in young rats and to 4.21 +/- 2.0 ng/ml in older rats. Shorter treatments were less effective. These data reveal a definite change in steroidogenic competence in older rat ovaries.


Stability of perceived tension as a function of the menstrual cycle. The stability of tension judgments among males and females who were either taking or not taking combination low-progestin oral contraceptive pills was investigated. Judgments of interpersonal pressure in videotaped vignettes provided tension measures. The vignettes were presented in two sessions separated by two weeks. Correlations between pressure judgments of .94 for males, .79 for pill females and .40 for non-pill females (all sig less than .002) supported the prediction that non-pill females experience the largest fluctuations in tension across the menstrual cycle. A significant number of non-pill females who changed their scores and who changed from midcycle to premenstrual-menstrual phases or vice versa between the two experimental sessions shifted their tension scores in the predicted direction (p less than .04). As predicted, non-pill females scoring in the lower half on premenstrual-menstrual symptomatology were more consistent in pressure judgments than those high on symptomatology, with correlations of .51 (sig less than .004) and .24 (NS) respectively. Analysis of pressure judgments as a function of menstrual phase and high-low symptomatology indicated a main effect for symptomatology (sig less than .01) with the high group perceiving more pressure. Lack of a main effect for menstrual phase was attributed to large individual variations in tension scores. Results were interpreted as indicating physiological causality underlying the variability in tension across the menstrual cycle. Substituting the Repression-Sensitization dimension for symptomatology yielded no significant results which could lead to a psychological interpretation of such variability.


Gnome and other effects of a small translocation in the mouse. A semisterile F1 male mouse from an X-ray experiment produced about 25 percent lethal gnome young in outcrosses. These animals were about half normal size, with short tail and small eyes, and died at birth. Surviving progeny were of four classes: 1) like the sire, 2) semisterile, 3) normal, and 4) gnome-producing, but not semisterile. Two independent reciprocal translocations have been identified from the original male, one of the classic type giving semisterile heterozygotes and involving chromosomes 5 and 15. The second translocation seems to be very small, giving the gnome type as one duplication-deficiency product, and the other unbalanced type seeming to pass for normal, although large body size and occasional agnathism may be produced. The small translocation has been found linked with the loci of v (waltzing) and Sl (steel) on chromosome 10. Cytological study has not revealed obvious structural changes. The translocation is now maintained in the homozygous state. The designation T(10;?)2Ho is proposed.


Concentration of oestradiol, progesterone, luteinizing hormone and follicle-stimulating hormone in the jugular venous plasma of ewes during the oestrous cycle. Changes in the concentrations of ovarian steroids and pituitary gonadotrophins were measured by radioimmunoassay in the jugular plasma of six Clun Forest ewes throughout the oestrous cycle. The concentration of oestradiol began to rise 12-14 h before the onset of oestrus from values of 11-2 +/- 0-36 (S.E.M.) pg/ml during the luteal phase to 21-1 +/- 2-01 pg/ml at -8 to 0 h (oestrus). There was no distinct increase during the luteal phase. Circulating progesterone varied in a cyclic manner with the highest values at the mid-luteal phase (3-70 +/- 0-28 ng/ml; n = 28). In five out of six ewes the concentration was still quite high (1-86 +/- 0-43 ng/ml) at 35 h before the onset of oestrus. The concentration declined rapidly thereafter, reaching minimum values about 12 h before oestrus coincident with the increase in oestradiol concentration. Plasma LH increased from very low values of 2-59 +/- 0-09 ng/ml during the luteal phase to 75-3 +/- 7-4 ng/ml about 9 h after the onset of oestrus. Two peaks of plasma FSH concentration were detected after the onset of oestrus. The first peak (171-0 +/- 35-5 ng/ml) coincided with the LH peak and the second (133-0 +/- 10-7 ng/ml) occurred about 24 h later at a time when LH values were low. The mean FSH concentration at other times during the cycle was 61-9 +/- 2-8 ng/ml.


Changes in aggressive and sexual responsiveness of male golden hamsters after neonatal androgen administration. On day 1 after birth, male golden hamsters received either 300 microng of an androgen (testosterone propionate, testosterone, dihydrotestosterone or androstenedione) in 0-03 ml arachis oil, or oil alone. As intact adults, their aggressiveness towards unreceptive females was measured. After this, all animals were castrated. At least 3 weeks after the operation all animals received oestradiol benzoate (10 microng) + progesterone (500 microng), after which their capacity to show patterns of female sexual behaviour towards a stud male was tested. Control hamsters which had received oil as neonates showed less aggression than the females with which they interacted; these controls also readily assumed lordosis after castration and priming with ovarian steroids. Conversely, animals which had received testosterone propionate or androstenedione neonatally were as aggressive as the female hamsters, and showed a markedly decreased ability to display lordotic behaviour after castration. The behaviour of male hamsters which received testosterone or dihydrotestosterone was unaffected. Thus, at the level of treatment used, increased aggressiveness appeared to co-vary with a decreased capacity to show female sexual behaviour patterns. However, within each treatment there was little evidence of such a relationship at the level of the individual animal.


Cytodifferentiation in the accessory glands of Tenebrio molitor I. Ultrastructure of the tubular gland in the post-ecdysial adult male. The tubular accessory reproductive glands of the male mealworm beetle consist of a secretory epithelium surrounded by a thin muscular sheath. Each columnar secretory cell is divisible into three zones: basal which is adjacent to the muscle layer and contains rough endoplasmic reticulum and Golgi, intermediate, which contains endoplasmic reticulum and Golgi zones in the immature gland and is filled with secretory vesicles in the mature gland, and apical. Maturation also involves proliferation and organization of the rough endoplasmic reticulum in the basal and intermediate zone. The process appears to be complete at four days after ecdysis. Parallels with other insect glands and with the mammalian prostate are striking.


Some effects of mating in female tsetse, Glossina austeni Newst. Virgin females of G. austeni were mated at carefully chosen physiological states to determine the effect of mating on ovulation, neurosecretion and blood meal size. The results suggest that ovulation and larviposition are controlled by neurosecretion, and that the release of neurosecretion requires both a mating stimulus and the presence of a mature egg in one of the ovarioles. The mating stimulus was also found to lead to an increase in blood meal size. Unmated female flies do not ovulate and their mature eggs eventually disintegrate. Virgin blood meal sizes remain relatively low.


Cyclic accumulation of zinc by Candida utilis during growth in batch culture. Intracellular accumulation of zinc by Candida utilis NRRL-Y-7634 was mediated by an energy-and temperature-dependent, highly specific process exhibiting saturation kinetics. In zinc-supplemented medium, uptake occured only during the lad and late-exponential phases; this type of transport did not occur with zinc in bacteria nor with iron in either yeast or bacteria. Cells of C. utilis did not possess a zinc-efflux system; they could reduce their level of intracellular zinc only by dilution of the metal into daughter cells. Zinc-deficient organisms accumulated 12 times more zinc than did cells of the same culture age grown in zinc-supplemented medium. The varied, but experimentally reproducible levels of intracellular zinc that occured in response to the physiological and environmental parameters had no detectable effects on respiration, rate of growth, total cell yield, or cell viability. Neither the mechanism underlying the cyclic accumulation of sinc nor the function of such behaviour are understood.


The applicability of inescapable shock as a source of animal depression. Male rats (N = 27) were given initial experience with escapable shock, equivalent amounts of inescapable shock, or no shock. Measures were then obtained in the ensuing 15 hours on food intake, water intake, number of cage crossings, and weight change for all groups. Following this, animals were tested on an escape task. Inescapably shocked animals showed significant decreases in food and water consumption in comparison to both nonshocked and escapably shocked control rats. Weight gains were significantly decreased by exposure to shock irrespective of the availability of a coping response. Consistency of these findings with proposals suggesting that exposure to inescapable shock leads to a state of animal depression (learned helplessness) is discussed and compared to alternative stress explanations.


Effect of dietary composition on hepatic lipid accumulation of rats with chronic ethanol intake. Diets higher in carbohydrate, fat or protein (diets 1, 2, and 3, respectively) were formulated isoenergetically with or without ethanol to study their effects on the accumulation of hepatic total lipids in rats fed for a period of 8 weeks. Ethanol ingestion did not affect body weight gain of rats fed diet 1, but diets 2 and 3 resulted in decreased weight gain as compared to the pair-fed controls. These body weight changes between control and ethanol groups were significant 2 weeks after beginning the treatment. Ethanol administration did not change hepatic weights of rats fed diet 1, but increased hepatic weights of rats fed diets 2 and 3. Higher protein alone in the diet increased liver weight. Ethanol intake increased the hepatic total lipid content of rats fed diets 2 and 3, but did not affect those fed diet 1 compared to their pair-fed controls. Hepatic cholesterol content increased in rats fed both the higher protein and higher fat diets. Both weight gain, liver weight, and hepatic total lipids consistently showed that the rats consuming 39% wheat starch as carbohydrate were not adversely affected by ethanol ingestion while those groups fed higher fat or higher protein with ethanol were adversely affected. Possible mechanisms are discussed.


Occurrence and survival of the larval nematode Anisakis sp. in the flesh of fresh, frozen, brined, and smoked pacific herring, Clupea harengus pallasi. Two hundred and twenty Pacific herring (Clupea harengus pallasi) from Yaquina Bay, Oregon, were divided into five groups to test the effects of various methods of handling and processing (fresh, frozen, brined, cold smoked, and cold smoked-gibbed) on the occurrence and survival of Anisakis larvae in the flesh. Viscera and flesh were digested separately using a pepsin-HC1 technique, and counts were taken of Anisakis larvae. Parasite loads were statistically largest in the flesh of frozen, brined, and smoked fish. Smoked fish harbored the greatest proportions of larvae in the flesh. Human consumption of brined or cold smoked Pacific herring represents a potential public health hazard.


Revision of Mediorhynchus van cleave 1916 (acanthocephala) with a key to species. Species characters in Mediorhynchus are discussed, and a consistent method of describing hook arrangement, longitudinal rows, is proposed. Mediorhynchus papillosus Van Cleave 1916, and M. orientalis Belopol'skaya 1953 are redescribed, and M. edmondsi sp. n. is described from Palawan. The new species is most similar to M. rodensis Cosin 1971 in armature, but differs from it in proboscis shape and overall size. Among other new records are M. gallinarum (Bhalerao, 1937) in chickens in Palawan, and M. turnixena (Tubangui, 1933) from a button quail in Palawan. Several synonymies are pointed out and several unidentifiable species are mentioned. A key to 29 species recognized as valid is provided.


Comparative activity and properties of lactate dehydrogenase, xanthine dehydrogenase, and dihydrofolate reductase in normal and Brugia pagangi-infected Aedes aegypti. The amount of xanthine dehydrogenase (XDH), dihydrofolate reductase (DHFR), and lactate dehydrogenase (LDH) in crude extracts of 4- to 5-day-old adult Aedes aegypti was determined, and the properties of these enzymes were partially characterized. It was then found that the amount and other selected characteristics of XDH and LDH in extracts of female Ae. aegypti processed 5 to 7 days and 12 to 14 days after they had fed upon either normal or Brugia pahangi-infected jirds were indistinguishable from those of these two enzymes in extracts of female mosquitoes that did not have a blood meal. Under the same circumstances, the selected characteristics of DHFR were also unaffected. However, there was a suggestion that the amount of DHFR was slightly increased in extracts of female Ae. aegypti processed 5 to 7 days after they had fed upon B. pahangi-infected jirds; by 12 to 14 days after the blood meal, there was a consistent 30% to 60% increase in the amount of DHFR inextracts of infected mosquitoes. DHFR activity could not be detected in a similarly prepared extract of 4,000 to 5,000 infective (L-3) B. pagangi larvae, the approximate number present in the infected mosquito extracts. It would appear, therefore, that the increased amount of turnover of DHFR in the mosquito host occurs in response to advanced infection with B. pahangi.


The breeding system of Polysphondylium pallidum, a cellular slime mold. A mating type analysis was performed on 231 isolates of the cellular slime mold, Polysphondylium pallidum found in 61 samples collected in eastern North America between northern Florida and sourthern Canada. Seventy-eight percent of the isolates belonged to one of 2 mating types; 18% were incapable of mating with any partner; 3% were homothallic; and 1%, consisting of 2 isolates from a Florida sample, belonged to a separate breeding group. It is suggested that the majority of isolates represent a species capable of local genetic adaptation to a niche, the parameters of which undergo considerable variation over space and time.


Life cycles of two isopora species in the canary, Serinus canarius Linnaeus. The endogenous stages of Isospora serini Arogão and Isospora canaria Box are described from experimentally infected canaries, Serinus canarius Linnaeus. Unlike other Coccidia, the first part of the I. serini life cycle takes place in mononuclear phagocytes. Five asexual generations are described from this cell type; 2 additional asexual generations and the sexual stages take place in the intestinal epithelium. Isospora canaria, on the other hand, has a conventional coccidian life cycle in that all of the endogenous stages are in the epithelium of the small intestine, with 3 asexual generations and the sexual generation described in the duodenal epithelium. The 2 species differ in their position relative to the nucleus of the intestinal epithelial cell. Isospora serini is usually on the lumenal side of the nucleus while I. canaria is below the nucleus, toward the basement membrane. The prepatent period is 4-5 days for I. canaria and 9-10 days for I. serini. Patency lasts for 11-13 days in I. canaria infections, but duration of oocyst output is more chronic in I. serini infections, persisting for as long as 231 days. Both species have a diurnal periodicity of oocyst discharge which occurs in late afternoon and evening.


Formation of amino acids and related oligomers from formaldehyde and hydroxylamine in a solution of transition metal ions. In a solution of transition metal ions with or without kaolin not only amino acids but also their oligomers were formed. The maximum ratio of amino acids after to before acid hydrolysis 5  6.


Insulin stimulation of fatty acid synthesis in human breast cancer cells. Four human breast cancer cell lines were tested for their response to hormones with respect to the lactational function, fatty acid synthesis. Physiologic concentrations of insulin enhanced the incorporation of [14C]acetate into fatty acids in two of four cell lines tested. All cell lines had specific, high-affinity insulin receptors; therefore, the failure of two lines to respond could not be attributed to the absence of receptor. The effect of insulin involved an increase in the maximun velocity of incorporation rather than a decrease in the Michaelis constant.


Cell-free coupling of influenza virus RNA transcription and translation. A cell-free coupled system for the transcription and translation of fowl plague virus RNA is described. The system utilizes a new nuclease-preincubated rabbit reticulocyte lysate that has a high sensitivity to exogenous mRNA and a very low level of nuclease activity. Translation of the viral proteins in the coupled system is strictly dependent upon the viral transcriptase activity. In the coupled system the optimal concentration of magnesium is intermediate between the optimum for transcription and that for translation. Translation of the viral proteins seems faithful. The products represent the major viral peptides M and NP and two peptides with the same electrophoretic mobility as HA and P2. Viron NA is not resolved in the kind of polyacrylamide gels described. Proteins M and NP were immunoprecipitable with monospecific antisera. It is concluded that the virion-associated RNA polymerase transcribes the negative-stranded segments of the viral genome coding for these major structural proteins into fully functional mRNA's.


Synthesis of Sindbis virus nonstructural polypeptides in chicken embryo fibroblasts. The identification of eight previously undescribed polypeptides in chicken embryo cells infected with Sindbis virus is reported. Seven of these polypeptides were distinguishable from the virus structural polypeptides and their precursors by their molecular weights and tryptic peptide maps. The eighth was closely related to pE2 (Schlesinger and Schlesinger, 1973), a precursor to one of the virus particle glycoproteins. Pulse-chase experiments and the use of an inhibitor of proteolytic cleavage allowed a division of the seven nonstructural (NS) polypeptides into three stable end products (NS p89, NS p82, and NS p60) and four precursors (p230, p215, p150, and p76). The labeling kinetics after synchronous initiation of translation indicated that synthesis of the NS polypeptides started at a single site and showed that the order of the genes coding for the NS polypeptides was (5' leads to 3') NS p60, NS p89, and NS p82. Short-pulse experiments under conditions of both synchronized and nonsynchronized translation suggested that cleavage of the primary translation product of the NS genes occurred only after its synthesis was completed and that the first cleavage removed the C-terminal polypeptide. From these and other experiments, we propose a detailed scheme for the synthesis and processing of Sindbis virus NS polypeptides.


Three unique viral RNA species of snowshoe hare and La Crosse bunyaviruses. Two-dimensional gel electrophoreses of RNase T1-derived oligonucleotides of the three individual RNA segments of the bunyavirus snowshow hare virus indicate that its three RNA segments possess distinct nucleotide sequences. The fingerprints of the RNA species of snowshoe hare virus differ from those of the antigenically closely related La Crosse virus. Three viral RNA species have been identified in preparations of Melao and Trivittatus as well as snowshoe hare, Lumbo, and La Crosse bunyaviruses.


Virus-directed post-translational cleavage in Sindbus virus-infected cells. The viral polypeptides synthesized in cells coinfected with group C and group D or E Sindbis virus mutants were studied. Cleavage of the ts2 protein occurs in cells coinfected with ts2 and ts20. Since the ts2 protein fails to chase in cells infected with ts2 alone, the activity effecting this cleavage must be, at least in part, virus specified.


RNA synthesized in calicivirus-infected cells is atypical of picornaviruses. RNA labeled with [3H]uridine from Vero cells infected with San Miguel sea lion virus in the presence of actinomycin D was analyzed by glycerol density gradient sedimentation and polyacrylamide gel electrophoresis. The predominant single-stranded RNA (36S, 2.6 x 10(6) molecular weight) was genome size. There was also a prominent 22S, 1.1 x 10(6)-molecular weight, single-stranded component and one or more double-stranded or partially double-stranded classes. Replicative forms, sedimenting at 18S, contained single-stranded RNA corresponding to the larger-molecular-weight class. All classes of intracellular RNA and virion RNA were polyadenylated. These findings and results with pig kidney cells infected with vesicular exanthema of swine virus and feline cells infected with feline calicivirus indicate that caliciviruses exhibit a strategy of replication different from typical picornaviruses and supports removal of the caliciviruses from the family Picornaviridae.


Contagious ecthyma in mountain goat of coastal British Columbia. Contagious ecthyma has been reported previously from mountain goat (Oreamnos americanus) in one restricted area of eastern British Columbia. A second focus of infection is reported for mountain goat from western British Columbia. Diagnosis was based on appearance of lesions at necropsy, histopathology and demonstration of poxvirus with the electron microscope. The epizootiology of this infection in mountain goat is discussed briefly.


An epizootic of waterfowl associated with a red tide episode in Florida. During February and March, 1974, an epizootic involving lesser scaup (Aythya affinis) occurred in the Tampa Bay area on the west coast of Florida. Several thousand ducks are estimated to have died. Concurrent with this epizootic was a red tide caused by heavy blooms of the toxic dinoflagellate Gymnodinium breve intoxication were evident in some of the lesser scaup. A controlled experimental feeding of G. breve toxic material to White Pekin ducklings produced illness and death with signs comparable to some of those seen in the scaup.


Physiological and clinical studies on norms and variations in counts of circulating basophile leucocytes. Variations in the counts of circulating basophile leucocytes in 225 subjects were studied. The results obtained are as follows: 1) The counts (per mm3) show a gradual rise with growth, from infancy to adolescence, and maintain a fair constancy in adults. 2) No significant sex difference was found in the peripheral basophile counts. 3) A definite diurnal variation in the counts exists, which is lowest in the morning and highest in the evening. 4) the counts are altered in different phases of the menstrual cycle. 5) Pregnant women show lower counts than nonpregnant women. 6) Surgical trauma and a single electroconvulsive shock lowers the counts significantly but variations are not significant after meals and physical exercise. 7) Smokers have slightly higher counts than non-smokers. 8) course of irradiation therapy lowers the counts significantly in patients with inoperable noplastic diseases. 9) Counts show insignificant variation with alteration of blood glucose level. The results mentioned above are discussed.


Hepatic aminopyrine N-demethylase system: further studies of assay procedure. Rat hepatic aminopyrine N-demethylase activity was measured by detecting the amount of formaldehyde produced from aminopyrine. Some optimal conditions for the N-demethylation were determined using both isolated microsomes and whole homogenates, and the standard assay method is described. Formaldehyde production from the substrate by microsomal enzyme system was linear to the amount of microsomes added during 3 min reaction time, whereas long-time incubation caused a decrease in the apparent activity of aminopyrine N-demethylation. The N-demethylase activity observed in normal rat liver homogenate was quite similar to that in microsomes when the activity was expressed on the basis of cytochrome P-450 as molecular activity. Pretreatment of animals with typical inducers, phenobarbital and 3-methylcholanthrene, resulted in alteration of the aminopyrine N-demethylase system, which was detectable in both microsomes and whole homogenates.


[Online CRT visual masking system].

[Online CRT visual masking system]. To analyse the human visual perception process by backward masking, thousands of TS's and MS's must be exposed even in an experiment. If a mechanical tachistoscope should be used, labors for making such stimuli, for changing them at each exposure and for arranging data of the S's reaction would be exceedingly great. In order to save the lavors, a computer-controlled visual masking system is designed. The computer generates the TS and MS, exposes them on the CRT and collects the S's reaction. A command language named OLM-1 is used to described forms of TS's and MS's and other parameters of backward masking. Using this system one thousand TS's can be exposed within an hour or so.


[Phakofragmentation and aspiration through the pars plana ciliaris (author's transl)]. Not until Machemer et al. started doing what they called posterior lensectomies with the vitreous-infusion-suction-cutter did removal of the lens through the pars plana become standard procedure. Effected originally as a first step in eyes undergoing vitrectomy, when an opaque lens interfered with fundus visibility, it has started to be used as an independant procedure in the case of soft-mostly traumatic-cataracts, and in that of ectopic or subluxated lenses. In 40 out of 43 operated cases the results have been as good as could be expected from the condition of the macula. Three cases, or 6.9%, developed serious complications: one a retinal detachment which could not be cured by surgery, one a chronic corneal edema, and one a secondary glaucoma which reacted poorly to medical therapy.


Morphologic changes during incubation of renal slices. The progression of ultrastructural changes was studied during various conditions of incubation of rat and mice renal cortex slices. The kidneys were sliced with a Stadie-Riggs microtome and incubated in various media (tris(hydroxymethyl) aminomethane (Tris), Tris-proline, and Hanks), in different oxygenation conditions (O2-CO2, 6 liters per minute; N2, 0.5 liter per minute), at different temperatures (37 degrees C, 23 degrees C.) and different time intervals (0 to 120 minutes). The most conspicious changes appear to involve plasma membranes and mitochondria. Early changes (0 to 30 minutes) are mainly confined to mitochondria cristae and matrix; there is also light swelling of the endoplasmic reticulum. After 30 minutes of incubation, the majority of mitochondria have a condensed form: dense matrix and slight dilation of the cristae. These modifications are more accentuated at 75 minutes and many mitochondria are swollen and contain dense material. The modification of the membrane takes place at 30 minutes by vesiculation and/or a diffuse dilation of brush border and is followed by myelinization. The antiluminal membrane appears less sensitive but the same modification pattern appears after 75 minutes. These changes appear more slowly at 23 degrees C. than at 37 degrees C. Kinetic studies were also carried out confirming findings of various authors; cellular uptake and accumulation of 14C-glycine still take place after 2 hours of incubation. The kidney cortex slicing technique does not seem to be suitable for the study of absorption at the luminal membrane. Furthermore, our observations suggest that the generally accepted ultrastructure of some organelles does not correspond to their appearance in the noramlly functioning state in vivo.


A light and electron microscopic study of experimental portal-systemic (ammonia) encephalopathy. Progression and reversal of the disorder. A sequential light and electron microscopic study of cerebral cortex was performed in a rat model of portal-systemic encephalopathy produced by creating a portacaval shunt and followed by ammoniate resin feedings. Prior to coma, astrocytes were characterized ultrastructurally by marked cytoplasmic enlargement, proliferation of mitochondria and endoplasmic reticulum, and an accumulation of cytoplasmic glycogen. The Alzheimer type II astrocyte change was seen only in coma and was characterized ultrastructurally by additional hydropic and degenerative mitochondrial and nuclear changes. Attempts at reversal of the encephalopathy were successful only if ammoniated resin feedings were discontinued prior to coma. Results suggest (1) that the astrocyte response initially reflects an ammonia-induced increased metabolic activity in that cell; (2) that subsequently a gliopathy develops having the light microscopic appearance of the Alzheimer type II change; and (3) that the Alzheimer type II astrocyte change may be responsible for an irreversible clinical course in this experimental condition.


Tobacco chewing an unusual case of drug dependence. This case report is that of a 53-year-old man without current evidence of significant psychiatric disorder who requested treatment for dependence on chewing-tobbaco. During his successful treatment in the Alcoholism and Drug Dependence Unit, we noted several similarities between his tobacco chewing and other drug dependencies.


[Formaldehyde determination in adhesives and in work environment (author's transl)]. A simple method is presented for analysis of urea-formaldehyde resins and other substances used in glueing operations, in relation to the environmental control of formaldehyde, considered as a pollutant in furniture plants.


PSRO and the dissolution of the malpractice suit. The effect of PSRO on the practice of defensive medicine and the effect of the civil immunity provision on the numbers of malpractice suits may well be substantial. Cost savings in terms of control of overutilization and of the potential for patient injury engendered by that overutilization will ensue. While substantial, those effects will be small compared to the effect of PSRO on the present medical malpractice system. As a social system, malpractice has two positive purposes. It serves as a measure of quality control on outcomes of medical care, and it serves to compensate people for injuries received in medically related situations. How well it serves these purposes is unknown, but what few data exist indicate that it does not significantly enhance the quality of care nor provide an efficient method of compensation. It exists in theory because it is the final check and balance on physician practice--the only existing control on the outcomes of care. Until some system other than malpractice could be conceived which was at least as efficient in outcomes control, malpractice was destined to continue, and no other method of patient compensation could be considered seriously since the compensation and quality control aspects of malpractice were so inextricably bound. With the advent of PSRO, another means of outcomes control came into existence, and as PSRO becomes operational nationwide, that system will prove an efficient and effective system of control over the quality of outcomes of medical care. Within the environment of PSRO, malpractice as a system of outcomes control has little reason to exist, and its worth as a system of patient compensation can be reexamined. It is within the framework of PSRO that other systems of compensation of the injured patient can be considered and developed--systems hopefully more just and more efficient than malpractice. This is the greatest impact PSRO will have on malpractice. It is, of course, a long-term process, and one which will not produce immediate results. In fact, the role of PSRO in effecting a change in patient compensation may never be recognized directly. It is only within the existence of a working system controlling the quality of outcomes of medical care that the injustices of the malpractice system can be righted and alternatives to that system can be considered.


Fatty acid compositions of lipid fractions from vegetative cells and mature sorocarps of the cellular slime mold Dictyostelium discoideum. A wild-type strain of Dictyostelium discoideum was grown upon Aerobacter aerogenes. Fatty acid compositions of lipid fractions and of total lipids obtained from vegetative amoebae and mature sorocarps were compared. Fatty acids isolated from vegetative cells were found to include large quantities of 17- and 19-carbon cyclopropane fatty acids while straight-chain, saturated fatty acids represented only 10% (w/w) of total fatty acids. These cyclopropane fatty acids appear to be derived from ingested bacteria and are preferentially incorporated into neutral lipids of the slime mold. Development of amoebae to mature sorocarps is accompanied by a substantial decrease in cyclopropane fatty acid content and a concomitant increase in unsaturated fatty acids, mostly as octadeca-5,11-dienoic acid. The delta-22 stigmastenyl ester fraction is the richest source of this acid. Fully 65% of the fatty acids in this fraction are the octadecadienoate.


Combination therapy for osteoporosis.

Combination therapy for osteoporosis. The effect of therapy that simultaneously stimulates bone formation and inhibits bone resporption was evaluated in five patients with primary osteoporosis. The technique of in vivo neutron activation analysis was used to measure total body calcium (TB-Ca). The patients were treated with calcium supplements and salmon calcitonin combined with either a low or a high dose of growth hormond (hGH). There was no significant increase in skeletal mass (TB-Ca) during the low-dose hGH regimen. An increase in skeletal mass was observed in all patients following the high-dose regimen, except for one patient who developed secondary hyperparathyroidism. Although this study must be considered to be of a preliminary nature, the magnitude of the response in calcium balance suggests that skeletal mass can be increased in osteoporosis if combination therapy is employed.


Modulative effect of endogenous granuloid inhibitors on cell proliferation. The particle-free crude extract of differentiated granulocytes (GCE) and the GI-1, GI-2, GI-3 proliferation inhibitory fractions (M.W. larger than or equal to 70,000 approximately 11,500 and less than or equal to 4000) were studied for the effects they exert on cultured cells. As shown by the curve, in bone marrow suspension cultures, in the dose range of 0.01-10.0 microng/ml, GCE maximally inhibits 3H-TdR incorporation into the DNA for 5.5 hours. In agar-gel cultures, 1.0-5.0 microng/ml of GCE reduces both the number (by 50.6-54.8%) and the size of the colonies formed. The manifestation of the inhibitory effect of GI-1, GI-2 and GI-3 fractions requires 2.5-3.5 hours. The inhibitors designated GI-2 and GI-2 are target-cell specific but they do not reduce 3H-TdR incorporation either in thymocyte suspension or HeLa monolayer cultures. On the other hand GI-1 inhibis the proliferation in HeLa cultures, as well. These endogenous inhibitors exert their optimum effect, even in a partially purified state at 2-4 orders or magnitude lower concentrations than does 1,2/5,6-dianhydrogalactitol (DAD), a cell-aspecific inhibitor used for comparison in the same system.


Clinical manifestations of tansy ragwort poisoning. The clinical signs of tansy ragwort poisoning are variable and in 5 cases included diarrhea, tenesmus, ascites, bloody feces, icterus, paresis, CNS involvement, rectal prolapse, poor appetite and weight loss. It is thus apparent that the disease can be confused clinically with many others, and tansy ragwort poisoning should be considered in animals exhibiting ascites, diarrhea and rectal prolapse. Several cases of tansy ragwort (Senecio jacobaea) poisoning occurring from 1969 to 1976 are reported to illustrate the numerous clinical pictures that might confront a practitioner. The disease is sporadic in this area. The poisonous principles in tansy ragwort are pyrrolizidine alkaloids, which cause gradual alteration and necrosis of liver cells with replacement by fibrous tissue. The development of signs in all of these cases could be attributed to failure of one or more liver functions.


Anisomycin sensitive mutants of Physarum polycephalum isolated by cyst selection. The haploid myxamoebae of Physarum polycephalum reversibly differentiate to form dormant microcysts under conditions of starvation. The thin-walled cysts can be selective recovered from a cell suspension which has been treated with the surfactant Triton X-100 to lyse amoeboid forms. Excystment, which is initiated by suspension in liquid medium, is inhibited by antibiotics which block protein synthesis. Cysts of drug resistant mutants excyst rapidly in media containing sufficient antibiotic to maintain drug sensitive strains in the encysted state. The selective survival of non-excysted cells following Triton X-100 treatment has been employed to enrich for drug sensitive mutants. Several anisomycin sensitive mutants have been isolated, one of which has been analysed genetically. The possible applications of this mutant enrichment technique are discussed.


Evidence against mutational "hot-spots" at aggregation loci in Dictyostelium discoideum. Complementation tests were performed on 10 strains of Dictyostelium discoideum which carry developmental mutations representing aggregation loci identified previously in two independent studies. When the 5 aggregation-deficient strains representing loci CGI-5 were fused with the 5 strains carrying mutations at loci ago A-E, all 25 crosses produced aggregation-competent diploids. Complicating factors, such as negative gene interactions and possible interallelic complementation are discussed. The results of this experiment suggest that the 10 aggregation loci identified in the two studies are different and that aggregation loci in D. discoideum are probably not associated with significant mutational "hot-spots".


Linkage analysis of developmental mutations in aggregation-deficient mutants of Dictyostelium discoideum. Simple parasexual genetic techniques have been employed to extend the linkage analysis initiated in an earlier study (Coukell, 1975) of developmental mutations (agg mutations) in 40 independently isolated aggregation-deficient mutants of Dictyostelium discoideum. Using these techniques, agg mutations in 28 of the 40 mutants have been assigned to 4 linkage groups: 16 in group II, 1 in group III, 10 in group IV, and 1 in group VI. None of the agg mutations analyzed appear to map in linkage group I. In addition, a new temperature-sensitive growth locus, designated tsgJ, was mapped in group III. It was also found that diploid strains of D. discoideum are readily induced to undergo haploidization when grown on 0.1% p-fluorophenylalanine (PFP) at 25.5 degrees C. Growth of diploid strains on PFP had no effect on the type of segregant classes obtained (i.e. PFP does not induce mitotic crossing-over), the subsequent growth and/or development of the segregants, or the ability of the segregants to reform stable diploids.


Mutational synergism between p-fluorophenylalanine and UV in Coprinus lagopus. Previous studies have shown that the amino acid analogue p-fluorophenylalanine (PFP) is mutagenic to Coprinus lagopus due to its incorporation into proteins [32]. Spontaneous mutations, PFP and UV mutagenesis and PFP/UV synergism have been studied in a UV resistant strain and in two complementing UV sensitive mutant strains. By comparison to the UV resistant strain, one UV sensitive strain shows normal spontaneous mutations, 1.4% PFP-induced mutations and 50-fold UV mutagenesis. The second UV sensitive strain has 19-fold spontaneous mutation frequency, 8% PFP induced mutations and slightly elevated UV mutagenesis. In all 3 strains the PFP/UV synergism is comparable (4--5 times the arithmetic expected). The results indicate that PFP mutagenesis is due to the incorporation of PFP into enzymes normally functioning in the organism but which also participate in UV repair mechanisms. A model is proposed for UV repair which is based on a PFP sensitive excision repair system of at least two enzymes, and alternative "error proof" pathway which is not suscetible to PFP and an "error prone" pathway which is responsible for UV mutagenesis and is susceptible to PFP as shown by the PFP/UV synergism. Because PFP is given before UV treatment, this implies a UV inducible cofactor and a PFP sensitive enzyme which only functions after UV activation.


Cerebral mucormycosis following experimental inoculation with Mortierella wolfii. A strain of Mortierella wolfii isolated from a case of bovine mycotic abortion in the United Kingdom did not produce disease in mice when inoculated by the intraperitoneal, intramuscular, intravenous or subcutaneous routes. When injected by the intracerebral route, it induced a chronic granulomatous infection of the brain resulting in hydrocephalus and eventually death.


Importance of free living mustelid carnivores in circulation of adiaspiromycosis. The study of adiaspiromycosis in 8 species of free living mustelid carnivores (266 specimens) revealed the average intensity of infection to be 41.4%. The highest incidence rate was found in the exoanthropic species Putorius eversmanni (73.1%) and Martes martes (72.2%) while the lowest was observed in the hemisynanthropic species Putorius putorius (30.6%). The stone marten (Martes foina)) is a new, still unknown reservoir host of C. parvum var. crescens, C. parvum for which Putorius eversmanni and mustela nivalis are new hosts, was also demonstrated in 3 cases. In the present paper, the role of mustelid carnivores in natural foci of adiaspiromycosis is discussed and evaluated. The importance of these predators in the circulation of C. parvum var. crescens is relatively wide. They make possible the liberation of adiaspores from the lungs of their prey - primarily small mammals - into the environment and participate in the spread of infection in both the horizontal and vertical directions. They play a part in the process of distributing of the organism to the vicinity of human dwellings, in the development of new elementary foci, and also act as important reservoir hosts of C. parvum var. crescens.


Familial spastic ataxia: occurrence in childhood. The childhood form of familial spastic ataxia differs in many aspects from the disease of adult onset but as yet has received little attention in the literature. Five children with familial spastic ataxia are presented. A general review of the pertinent literature on familial spastic ataxia is included. The clinical and pathologic features of the childhood form of this disease are variable. Because this recessive disease has no known metabolic marker, differential diagnosis is difficult, requiring detailed history and careful observation.


Acute cerebellar hemorrhage. Analysis of clinical findings and outcome in 12 cases. Although a majority of reported cases of cerebellar hemorrhage are subacute or chronic, an acute form of cerebellar hemorrhage occurs that results in coma within 48 hours of onset and is probably always fatal without surgical intervention. Our experience with 12 consecutive patients with proved acute cerebellar hemorrhage is summarized. Of three patients treated with aggressive medical therapy alone, none survived more than 48 hours. In seven of nine operated cases, emergency surgery was undertaken solely on the strength of clinical diagnosis without radiologic confirmation. Three died postoperatively. Of six survivors, two recovered fully, and two show mild and two moderate residua. The major factors influencing survival were the rate of evolution of signs and the level of consciousness at the moment of surgery.


Alexia without agraphia in a left-handed patient with prosopagnosia. A left-handed patient was studied who had the acute onset of alexia without agraphia, a left homonymous hemianopia, and prosopagnosia. Neurodiagnostic tests including computerized axial tomography and angiography disclosed bilateral lesions in the posterior cerebral hemispheres involving the splenium of the corpus callosum. Neuropsychologic examination indicated marked impairment of facial discrimination, deficient naming and memory of presented visual material, and mild visual agnosia with relative preservation of other cognitive functions. These findings are consistent with a double disconnection syndrome involving disconnection of the dominant angular gyrus and right inferotemporal cortex from their bilateral visual inputs.


Failure of inactivation of Duchenne dystrophy X-chromosome in one of female identical twins. Duchenne muscular dystrophy manifested in one of girl twins. The twins were monozygous on the basis of red cell and HL antigens and skin graft compatibility. Karyotyping, including banding techniques, showed a normal number of chromosomes and a normal configuration of the X-chromosome in both twins. The twins were identical in appearance until symptoms of Duchenne dystrophy developed in  one at age 4 years. The maternal uncle had classic Duchenne dystrophy; the mother and the nonmanifesting twin showed evidence of being heterozygous for Duchenne dystrophy. The phenotypic difference in monozygous twins is readily explained by lyonization of the X-chromosome after twinning has occurred. The findings substantiate the existence of Duchenne dystrophy manifesting in females with normal karyotypes.


Nonhypoxemic hazards of prolonged myoclonus. Hyperkalemia, hyperthermia, and systemic hypotension developed in a patient with generalized myoclonus. These disorders reached life-threatening proportions but were rapidly and completely corrected after the patient was paralyzed with pharmacologic agents. Induced neuromuscular blockade may be indicated when the systemic effects of pathologically contracting muscle become life-threatening.


Myasthenia gravis and myotonic dystrophy in a 13-year-old girl. G. Milton Shy Award Essay, 1976. A 13-year-old girl demonstrated clinical, pharmacologic, and electromyographic evidence of myasthenia gravis, as well as clinical, electromyographic, and genetic evidence of myotonic dystrophy. Although myotonic dystrophy was documented in the family, no other family members were found to have myasthenia gravis.


Effective treatment of infantile myasthenia gravis by combined prednisone and thymectomy. Myasthenia gravis developed in two children at 2 and 3 years of age respectively. Minimal improvement followed chronic oral administration of cholinesterase inhibitors. Patient 1 had ptosis and ophthalmoplegia but no clinical or electromyographic involvement of muscles of the extremities, although a quadriceps muscle biopsy revealed lymphorrhages. Patient 2 had progressive generalized myasthenia for 3 1/2 years. Both children were given a 3-month course of prednisone followed by thymectomy. They both are in remission, 12 and 8 months after thymectomy, with only minimal residual ocular weakness, but this weakness is much more responsive to anticholinesterase drugs than before thymectomy. Long-term administration of steroids, with the attendant complication of growth retardation, is avoided.


Retrobulbar neuritis. In vitro evidence of sensitization to myelin basic protein in patients without multiple sclerosis. Thirty-six normal subjects and 34 patients with retrobulbar neuritis were studied with use of the technique of macrophage migration inhibition factor assay and myelin basic protein as antigen. Serial studies were carried out when possible. Normal subjects gave a mean migration index of 100.9+/-9. Eleven patients with retrobulbar neuritis alone gave a mean migration index of 55+/-16 in the first 3 weeks of illness, 89+/-17.3 during the fourth to the twenty-fourth weeks, and 100.9+/-9.0 after the twenty-fourth week. Ten multiple sclerosis patients with retrobulbar neuritis gave values of 61+/-21 in the first 3 weeks of an attack and 92+/-22.8 during the fourth to the twenty-fourth weeks, and 12 other multiple sclerosis patients 24 weeks or longer after an attack gave a value of 101.9+/-12.6. In a mean follow-up period of 1.9 years, only two patients presenting with retrobulbar neuritis alone have had a diagnosis of multiple sclerosis established; three others have weakness and reflex change in one limb only; and four have minor psychiatric problems. One retrobulbar neuritis patient has a family history of multiple sclerosis, but has no neurologic abnormalities. Comparison of these studies in both groups shows no statistical differences and supports the concept that cell-mediated hypersensitization to central nervous system myelin basic protein, however initiated, is a factor in the pathogenesis of retrobulbar neuritis.


Multiple neuroepithelial (colloid) cysts: association with other congenital anomalies. A 4-year-old boy had three neuroepithelial cysts, one in the third ventricle and two in the supratentorial subarachnoid space, and also had stenosis of the cerebral aqueduct, obstructive internal hydrocephalus, frontoethmoidal meningoencephalocele, and right microphthalmia. The occurence of these combined lesions supports the congenitially derived nature of the cysts. The cysts originated from neuroepithelium including ectopic ependyma-lined structures. The exception may be those cysts arising in or near the floor of the third ventricle; these cysts could be derived from the cleft of Rathke's pouch, an endodermal derivative. Folding of the neuroepithelium in contact with mesenchyma or neuroglial tissue is proposed as the mode of formation of most "colloid" cysts. Review of the literature as well as our experience indicates that neuroepithelial cysts are widely distributed throughout the central nervous system.


Epileptogenic action of intraventricularly injected antimelatonin antibody. In rats, intraventricularly injected antimelatonin antibody caused the appearance of transitory epileptiform abnormalities. Epileptic activity arose from and was limited to the cortical mantle of the hemisphere ipsilateral to the injection side. The occasional occurrence of lateralized seizures has also been observed. Control injection of saline, of the vehicle (rabbit serum), or of antibody saturated with melatonin induced flattening and desynchronization of the electroencephalogram but not epileptiform activity. Repeated antimelatonin injections caused reappearance of the same type of epileptic abnormalities that lasted slightly longer than the first time. Melatonin may play an inhibitory role in neuronal excitability.


Progressive cerebellar ataxia, spasticity, psychomotor retardation, and hexosaminidase deficiency in a 10-year-old child: juvenile Sandhoff disease. During the course of investigating a 10-year-old boy because of progressive deterioration of intellectual functioning, ataxia, and hemiplegia, an absence of serum hexosaminidase activity was noted. A skin biopsy examined by electron microscopy showed axonal accumulations of dense osmiophilic deposits. Because of the patient's age at onset and the slowly progressive nature of his ilness, we are reporting an atypical juvenile case of Sandhoff disease.


The electroencephalogram in childhood basilar artery migraine. In two pediatric patients with the clinical picture of basilar artery migraine, transient electroencephalographic disturbances were seen. Both children manifested posterior rhythmic delta activity in close temporal proximity to their attacks. Resolution of these abnormalities was documented by serial electroencephalographic tracings. These electroencephalographic findings are consistent with transient dysfunction in the neural structures supplied by the basilar artery and its branches. Pediatric patients in whom there is a clinical picture of basilar artery migraine and who have a family history of migraine and transient electroencephalographic changes of the type herein described need not undergo invasive neurodiagnostic study.


Herpes simplex infection and experimental allergic encephalomyelitis. An experimental model system for reactivation of EAE. The effects of herpes simplex virus type 1 (HSV-1) infection on the course of experimental allergic encephalomyelitis (EAE) were studied in rats. Fifty percent of animals given two intracerebral injections of HSV-1, one before and one after induction of EAE, showed clinical and pathologic evidence of recently exacerbated EAE 16 days after the second HSV-1 injection. When HSV-1 injections were administered subcutaneously before and after induction of EAE, 45% of survivors showed pathologic changes of recent EAE. A single injection intracerebally or subcutaneously of HSV-1 given before the development of EAE did not change the clinical severity or time course of EAE. A single injection intracerebrally or subcutaneously of HSV-1 given after the development of EAE did not cause clinical recrudescence of the EAE. Pathologic but not clinical evidence of EAE recurrence was found in three of nine animals given one injection of HSV-1 intracerebrally before and one of control material intracerebrally after induction of EAE. Pathologic evidence of EAE recurrence was found in six of 14 rats given one injection of control material intracerebrally before and one of HSV-1 intracebrally after induction of EAE. Cell suspensions, free of HSV-1, given prior and subsequent to the development of EAE did not cause a change in the EAE severity or a recrudescence of the EAE.


Granulomatous angiitis of the brain: a successfully treated case. A patient with clinical and radiographic findings initially suggesting a neoplasm was diagnosed as having Hodgkin disease. Computerized axial tomography demonstrated profound diffuse decreased density similar to that seen in cases of cerebral edema or leukomalacia. Cerebral angiography showed blood vessels with a beaded appearance. A diagnosis of granulomatous angitis was made on the basis of brain biopsy specimens. Viral cultures were negative. Skin testing revealed poor response to antigens. The patient improved dramatically following a combination of radiotherapy and steroid therapy. Resolution of the low-density abnormally seen on serial EMI scanning and resolution of the vasculitis demonstrated in the post-therapy brain biopsy specimen was dramatic. This is the first case of granulomatous angitis of the brain diagnosed during life, and it points out the value of radiation and steroid therapy for relief of neurologic symptoms.


Normal cerebral arteriography in patients with spontaneous subarachnoid hemorrhage. Previous studies have reported that 18 to 27% of patients with spontaneous subarachnoid hemorrhage have normal cerebral arteriograms. Our series of 220 patients from three hospitals demonstrated normal arteriograms in only 16 (7%). Recent improvements in neuroradiologic techniques, such as femoral catheterization, magnification angiography, oblique and basal projections, and subtraction most probably contribute to the improved yield. Nonvisualization of a cerebral aneurysm probably represents the most common explanation for a normal cerebral arteriogram.


Inhibition of leukocyte chemotaxis by homogenates of tumor tissues. Homogenates of human cancer tissues were assayed for chemotactic activity in modified Boyden chambers utilizing polycarbonate filters with an 8micron pore size. Peritoneal exudate cells (PEC) of guinea pigs were used as indicator cells in the assays and the chemotactic responses of both eosinophilic and neutrophilic leukocytes were recorded. The chemotactic responsiveness of leukocytes to the majority of homogenates was significantly less than that observed with the negative controls. It is suggested that the anti-chemotactic activity observed in vitro may operate in vivo to abrogate inflammatory responses and tumor rejection.


Invasion of malignant cells into 51Cr-labeled host tissues in organotypical culture. We have attempted to quantitate malignant invasion in vitro. Therefore we adapted the 51Cr-release cytotoxicity test to organotypical culture systems, that proved useful for morphological analysis of invasion. We counted 51Cr-release from labeled fragments of embryonic chick heart and mesonephros, cultured either on semi-solid or in fluid medium, during their invasion by HeLa cells. Although demonstrated morphologically, malignant invasion did not bring about significant changes of 51Cr-release as compared to control cultures without HeLa cells. The failure of the 51Cr-release test in our experimental device might shed some light on the mechanisms of malignant invasion.


Neonatal red cell superoxide dismutase enzyme levels: possible role as a cellular defense mechanism against pulmonary oxygen toxicity. Red cell superoxide dismutase (SOD) enzyme activity was determined in 58 cord blood specimens obtained from infants over a range of gestational ages. An inverse relationship between red cell SOD activity and gestational age was demonstrated. Red cell SOD activity showed a progressive fall from 263.1 +/- 30.5 units/mg non-hemoglobin protein (NHP) in infants of less than 29 weeks of gestation to 168.9 +/- 21.3 units/mg NHP in infants of more than 36 weeks of gestation (P less than 0.05). Infants treated for RDS showed an increase in red cell SOD activity which reached significance at 72 hr when compared to cord blood levels from the same population (P less than 0.05). No similar significant difference could be demonstrated in gestational age-matched control subjects over the same time period. However, initial cord blood SOD enzyme levels were lower in premature infants with RDS (229.5 +/- 30.6 units/mg NHP) than in premature infants without RDS (264.0 +/- 38.0 units/mg NHP). When infants with RDS were examined for oxygen toxicity and survival, red cell SOD levels were noted to decrease over 24 hr in four of five infants who died, three of whom developed bronchopulmonary dysplasia. In the surviving infants, red cell SOD levels showed a significant increase by 48 hr (P less than 0.05). None developed bronchopulmonary dyplasia and all survived.


Phototherapy for neonatal jaundice in erythrocyte glucose-6-phosphate dehydrogenase-deficient infants. The effectiveness of phototherapy in the management of neonatal hyperbilirubinemia in glucose-6-phosphate dehydrogenase (G6PD)-deficient infants was studied. "Prophylactic" phototherapy for six continuous days commencing from the first day of life was effective in preventing a significant rise in bilirubin levels in 12 G6PD-deficient infants in the first three days, during which period a rapid rise was observed in a control group of G6PD-deficient infants. The hemoglobin levels on the first and eighth postnatal days were comparable in both groups. "Therapeutic" phototherapy proved equally effective in reducing bilirubin levels in 24 infants with nonhemolytic hyperbilirubinemia and an equal number of infnats with hyperbilirubinemia associated with G6PD deficiency. Phototherapy was efficacious in the prevention or treatment of neonatal hyperbilirubinemia associated with G6PD deficiency; even if its use is prolonged it does not cause hemolysis in such infants.


[Cleavage and gastrulation in Microsomacanthus paramicrosoma (Cyclophyllidea, Cestoda)]. On the basis of hystological studies a description of fission and gastrulation in Microsomacanthus paramicrosoma (gasowska, 1931) is given. Eggs lacking morphological characters of polarity and symmetry display features of duet and spiral fission characteristic of lower groups of Turbellaria. Serial sections (5 mcm thick) have shown that the fission ends in the formation of coeloblastula consisting of 26 +/- 3 cells. Gastrulation is expressed in immigration into blastocoel of two micromeres which later degenerate. These micromeres are homologous to entomesodermal micromeres of other Platodes. Blastocoel disappears as a result of local reproduction of ectodermal cells that is regarded as an anlage of ectomesenchyma. Ectoderm and mesenchyma are not morphologically separated from each other.


Response-selection processes in paranoid and nonparanoid schizophrenia. Previous research (Marshall, 1973) has shown that the most pronounced component of deficit on a choice reaction time task among a mixed schizophrenic sample involved response-selection processes. Other evidence has indicated that paranoids may be more deficient in this respect than nonparanoids. Hence, it was hypothesized that the former subgroup of schizophrenics would display response-selection deficit while the latter subgroup would display either less or no deficit. Response-selection processes were re-examined using the CRT paradigm with comparisons carried out among paranoid and nonparanoid schizophrenics and a group of nonschizophrenic controls. Results indicated that only the paranoid schizophrenics displayed abnormally retarded response-selection operations, the nonparanoid schizophrenics being nonsignificantly discriminable from the controls. It was suggested that past evidence of CRT response-selection deficit among mixed schizophrenics might have been attributable primarily to the performance of the paranoids, whose performance appears to be adversely affected by an increase in the number of dimensions relevant to response selection.


Effect of splanchnicotomy on the renal excretion of uric acid in anaesthetized dogs. The renal excretion of uric acid was examined during acute i.v. urate (Ua) loading on unilaterally splanchnicotomized ("renal denervation") anaesthetized mongrel dogs. Glomerular filtration rate (GFR) in general was not different in innervated and denervated kidneys, whereas urine flow (V) and urinary excretion of sodium (UNaV) on the splanchnicotomized side were significantly increased at any plasma concentration of Ua. The excretion (UUaV) and tubular transport (TUa) of urate calculated for unit GFR were considerably increased and depressed, respectively, at normal plasma Ua level and during minor urate loading (plasma concentration up to 4.7 mg%). Above this plasma level, i.e. up to 24.6 mg%, no difference in net urate reabsorption between intact and sympathectomized organs was found. It is suggested that both reabsorption and secretion of Ua in denervated kidneys are diminished.


Effects of aldosterone on lipid metabolism and renal oxygen consumption in the rat. The plasma level of free fatty acids (FFA) in adrenalectomized rats increases by 50% after treatment with aldosterone (2 microng/100 g rat). Lipolytic activity in peripheral fat tissue is lowered after adrenalectomy and doubles after in vivo administration of aldosterone to adrenalectomized rats (measured as free fatty acid release in vitro from epididymal fat tissue). Lypolysis of adipose tissue stimulated by the in vitro presence of ACTH also increases after in vivo administration of aldosterone. Incorporation of intravenously administered label from U-14C-palmitate into total extractable lipid of renal tissue is augmented 3 h after aldosterone administration to adrenalectomized rats, while no increase of the radioactivity is observed in total lipid from liver tissue. Treatment with aldosterone does not affect the total lipid content of kidney or liver in adrenalectomized rats. The oxygen consumption rate of kidney cortex slices with lactate, beta-hydroxybuterate or acetoacetate as substrates is lowered after in vivo administration of aldosterone to adrenalectomized rats. With slccinate, however, the respiratory rate of kidney slices increases after aldosterone treatment of adrenalectomized rats, the ouabain-sensitive respiration being more affected than the ouabain-insensitive respiration. An interpretation of the O2 consumption data implicating competition of lipid metabolism for CoA-SH is discussed.


Effect of contraction on lymphatic, venous, and tissue electr-lytes and metabolites in rabbit skeletal muscle. The effect of muscle contraction on lymphatic and plasma [K+], [Na+], [Ca2+], [Mg2+], [Cl-], [Pi], [lactate] ([Lac-]); [creatine] ([Cr]), ideal osmolality (OSM), and [protein] was evaluated in femoral venous blood and lymph specimens sampled from the calf muscles of rabbits before, in the course of, and after contractions. In addition, total [K+], [Na+], [Ca2+], [Mg2+], [Cl-], and [H2O] were analyzed in the muscle tissue. To facilitate lymph sampling both hind limbs were passively flexed and extended, in imitation of natural running movements, by an electrically driven crank. The muscles of one side also performed superimposed rhythmic isotonic contractions. Before contractions, lymphatic [K+], [Na+], [Ca2+], [Mg2+], [Lac-], [Cr], and OSM did not significantly differ from corresponding femoral venous concentrations, [Cl-], and [Pi] were significantly higher, [protein] significantly lower in the lymph than in the plasma. During contractions lymphatic [K+], OSM, [Lac-], and [Pi] were raised significantly more in the lymph compared with the plasma concentrations. [Na+], [Cl-], [Ca2+], and [Mg2+] showed only small changes in the course of contractions and thereafter, and they were altered in a similar way in the lymph and plasma. It was suggested that lymphatic and interstitial concentrations were in equilibrium. Comparing inactive with active muscles, the latter lost K+ but gained Na+, Cl-, and H2O, whereas minimal changes occurred in the [Ca2+] and [Mg2+]. The changes were discussed in connection with the hypothesis that electrolyte shifts might be involved in the activation of the muscular non-proprioceptive interstitial nerve endings which appear to play a role in reflexogenic cardiovascular and respiratory control.


Studies on the lithium transport across the red cell membrane. III. Factors contributing to the intraindividual variability of the in vitro Li+ distribution across the human red cell membrane. 1. Extracellular potassium bicarbonate, ouabain, dipyridamole and the Na+ distribution between red cells and plasma influence Li+ transport across the human red cell membrane. The significance of these parameters for the intraindividual variability of the steady-state ratio of external to internal Li+ was studied in vitro. 2. Elevation of external K+ in the physiological concentration range increases the steady-state distribution ratio Lie+/Lii+ indirectly by increasing the ratio Nae+/Nai+ through activation of the Na+-K+ pump, and directly by inhibiting ouabain-sensitive Li+ uptake. 3. A rise in bicarbonate concentration decreases the Li+ ratio directly by accelerating Li+ uptake through a leak, and indirectly by increasing the Na+ leak, thus reducing the Na+ ratio. 4. Dipyridamole blocks both bicarbonate effects. 5. Ouabain decreases the Na+ ratio and inhibits Li+ uptake by the Na+-K+ pump, thereby exerting two opposite effects on the Li+ distribution ratio. 6. The results confirm the previous observation that the steady-state Li+ distribution depends strongly on the Na+ distribution ratio, i-e., the driving force for Na+-dependent Li+ uphill countertransport. It is concluded that the Na+ distribution between red cells and plasma and the concentrations of K+ and bicarbonate in plasma need to be considered as factors influencing the in vivo Li+ distribution. However, the considerable interindividual differences of Li+ distribution cannot be ascribed to variations in these parameters.


Extracellular fluid volume and central circulation after long lasting exercise and dehydration in conscious dogs. Two aspects of the recovery period after endurance exercise were investigated: a) the fluid distribution between the intra- and extravascular parts of the extracellular fluid volume (ECFV) induced by exercise dehydration, b) the cardiovascular response pattern [blood pressure (BP), heart rate (HR), cardiac output (CO), total peripheral resistance (TPR), and central venous pressure (CVP)] to the heat load which results from the preceding exercise. Seven conscious dogs performed endurance exercise in a cool environment (16 degrees C) on a horizontal treadmill till 4% of the body weight was lost. It was found that about 70% of the total fluid loss of the body came from intracellular water. During exercise sodium and chloride concentrations rose by 6 mMol and 7 mMol respectively (P less than 0.005) and remained elevated throughout the early recovery period indicating a fluid loss of about 100-200 ml out of the ECFV. Direct measurements of the ECFV as sulfate space confirmed these values. Since the plasma volume remained unchanged, this fluid loss was carried totally by the interstitial fluid volume. Immediately after exercise body temperature was elevated by 1.5 degrees C and returned towards control within 90 min. Cardiac output was above control level for 2 h after the end of exercise, at first due to an increased HR and thereafter to an elevated stroke volume (SV) (P less than 0.02). CVP and TPR were below control levels for at least 2 h (P less than 0.01). A linear correlation was found between CVP and TPR. A close correlation existed between the body temperature and the cardiovascular parameters. It can be concluded that even long after exercise the cardiovascular system has to serve thermoregulatory needs.


Time-dependent changes in the rate of noradrenaline synthesis in various rat brain areas during cold exposure. The synthesis of noradrenaline (NA) was studied in vivo in the hypothalamus, the brain stem and the "rest of brain" of rats which were exposed to 4 degrees C for 0.5, 2.5 or 24 h. The rate of NA synthesis was estimated 30 min after an i.v. injection of 3H tyrosine (TY) by evaluation of the ratio: 3H-NA specific activity/3H-TY specific activity. Cold exposure did not have the same effect on NA synthesis in the three brain areas. In the hypothalamus, the rate of NA synthesis was increased by a factor of 1.7 and 2 after 0.5 and 2.5 h of cold exposure, respectively and returned to control values after 24 h of cold exposure. In the brain stem, NA synthesis was enhanced by a factor of 1.5 at 2.5 h and returned also to control values at 24 h. In the "rest of brain", cold exposure did not alter NA synthesis at the three intervals studied. These results indicate that the activation of central noradrenergic neurones by cold is only a transient response which is restricted to specific brain areas and which can be interpreted as the consequence of an initial general stress associated with cold exposure.


A comparison of the electrical properties and morphological characteristics of the smooth muscle of the rat and guinea-pig vas deferens. Microelectrodes were used to compare a variety of electrophysiological parameters of the rat and guinea-pig vas deferens. In comparison to the guinea pig, spontaneous junction potentials in the rat tissue were of shorter duration and occurred with greater frequency and amplitude. Action potentials induced by nerve stimulation could be observed in the smooth muscle of both species. However, in the rat tissue the majority of action potentials were generated in the impaled cell while 60% of the action potentials in the guinea-pig vas deferens were propagated. When current was intracellularly applied, spike potentials could be induced in approximately 90% of the cells of the rat vas deferens but in less than 10% of the cells of the guinea-pig vas deferens. The space constant was 1.48 mm for the guinea-pig vas deferens, but less than 0.5 mm for the rat vas deferens. Electromicroscopic examination of the homologous tissues indicates that the differences in electrical properties can be accounted for in part by differences in morphology. The incidence and intimacy of neuromuscular contacts was greater in the rat vas deferens while the incidence of nexuses between smooth muscle cells was greater in the guinea-pig tissue.


Effects of verapamil on excitation-contraction coupling in single crab muscle fibers. The effects of verapamil and its optical isomers on the electrical and mechanical characteristics of single muscle fibers of Callinectes danae were studied. Verapamil (10-20 microng/ml) blocked the procaine- and TEA-induced spikes; the blockade was preceded by reduction in the rate of rise of the upstroke and increase in the duration of the action potentials. Inhibition of Ba-spikes required higher concentrations of verapamil (greater than 50 microng/ml). These concentrations reduced the amplitude of the normally occurring graded electrogenic membrane responses and reduced the rate of development of the current-induced tensions. With lower concentrations (10-30 microng/ml) verapamil enhanced the negative afterpotentials and the peak amplitude of the local contractions elicited by depolarizing current pulses, while the graded membrane responses were not markedly modified. Verapamil (1-100 microng/ml) did not affect the resting membrane potential but increased the effective membrane resistance. Determination of the cable characteristics by DC pulses indicated that verapamil (1-10 microng/ml) shortens the membrane length constant, increases the specific resistivity of the sarcoplasm and, in most cases, increases the membrane time constant. Verapamil (10 microng/ml) induced tension in these crab fibers. The contractions were potentiated in Na-deficient media, by increase in [Ca]0, and by membrane depolarization; "Ca-free" salines depressed, and procaine abolished these contractions. The results suggest that verapamil affects both Ca and K conductances and interferes with the Ca-sequestering mechanisms of these fibers. The (-)-isomer of verapamil was more effective than the (+)-isomer with respect to tension development, prolongation and subsequent blockade of procaine-spikes and enhancement of current-induced after-potentials and contractions.


Intravascular heat exchanger for conscious goats. Polyethylene tubings were chronically implanted into the vascular system of goats and served as heat exchangers to remove heat directly from the body core at a rate equalling several times resting heat production.


[Treatment of arterial hypertension with propranolol in pregnant women. 10 cases]. Ten hypertensive patients were treated with propranolol throughout pregnancy. Propranolol had a favorable effect on blood pressure and perhaps on other signs of pre-eclampsia too. No increase in uterine contractility, causing premature delivery, was noted. Weight at birth and APGAR index in infants was apparently not unfavorably influenced. Cardiac rhythm and blood glucose levels at birth were not studied. Theoretical risks of beta-blockers during pregnancy (premature delivery, danger to the foetus) may be overestimated and should be reevaluated in a prospective way.


Interactions of Hg(II) ions with DNA as revealed by CD measurements. The circular dichroism spectra of Hg(II) complexes with native calf thymus DNA, chemically methylated Streptomyces chrysomallus DNA and with Ag(I)-DNA complexes were measured in the region of 220 - 340 nm. As a main result a conversion of the conservative CD spectrum of DNA to a distinct nonconservative type of CD spectrum for the complexes occurs with increasing Hg(II) concentration. The CD spectra of the Hg(II) complexes as well as some additional arguments strongly support the idea, that DNA in the complex undergoes a structural transition to a more condensed state with 4 -like character.


Transcriptional organization of the 5.8S ribosomal RNA cistron in Xenopus laevis ribosomal DNA. Hybridization of purified, 32p-labeled 5.8S ribosomal RNA from Xenopus laevis to fragments generated from X. laevis rDNA by the restriction endonuclease, EcoRI, demonstrates that the 5.8S rRNA cistron lies within the transcribed region that links the 18S and 28S rRNA cistrons.


Purification of uricase by biospecific adsorption-desorption. A simple procedure for the purification of uricase from bovine kidney is described. The procedure involves the following steps: 1) processing of kidney mince by borate/butanol, 2) ammonium sulphate precipitation, and 3) biospecific adsorption-desorption. The adsorbents were prepared by chemical attachment of urate or xanthine to agarose gel beads. The desorption was performed by a xanthine solution. The adsorption-desorption procedure resulted in an 11 000-12 000-fold purification. The specific activity of the purified uricase was 19.8 U/mg using either "urate" adsorbent. The recovery was about 70%. The adsorbents were also used for the purification of commercial uricase preparations from hog liver. In this case the purified uricase also possessed a specific activity of 19.8 U/mg. The products were homogenous as judged by gradipore electrophoresis and gel filtration.


A method for isolation of 2S, 7S and 11S proteins of soybean. A method is described for the isolation of 2S, 7S and 11S proteins of soybean. The unfractionated proteins are first precipitated with Mg (11) which yields a 11S-rich precipitate and this is further purified by (NH4)2SO4 precipitation. From Mg(11) supernatant which is rich in 7S and 2S proteins, they are separated by the use of (NH4)2SO4 and cold ethanol. The 7S and 11S protein are obtained in a highly purified form as indicated by ultracentrifugation and polyacrylamide gel electrophoresis.


[Influence of aerobic and anaerobic conditions on the chemical composition and enzyme activity of the buds of mother cells of Candida utilis IBFM-405 yeasts]. The effect of aerobic and anaerobic conditions on the chemical composition and enzyme activity of buds and mother cells of yeast Candida utilis IBPM-405 was studied. Upon transition from aerobic to anaerobic conditions buds and mother cells of yeast Candida utilis IBPM-405 showed significant changes in the qualitative composition of exchange pool of free intracellular amino acids and quantitative content of its individual components. The transition did not influence the amino acid composition of proteins. Upon the transition from aerobic to anerobic conditions the activity of phosphorylase, glucose-6-phosphatase andn glutaminase in mother cells increased and in buds decreased, inadequate changes in the activity of aminoacyl-tRNA-syntheses occurring.


Metastic tumor presenting as intracerebral hemorrhage. Report of 6 cases examined by computed tomography. Six patients with intracranial metastases were studied with computed tomography (CT). The initial appearance suggested cerebral hemorrhage. Atypical location, contrast enhancement, or multiplicity proved helpful in differentiating tumors from other causes of intracerebral hemorrhage. Four patients showed contrast enhancement and 3 had multiple lesions. Metastases with associated intracerebral hematoma may simulate a spontaneous hematoma.


The endocrinology of testicular tumors.

The endocrinology of testicular tumors. The endocrine manifestations of testicular tumors can be produced by one of three mehcansims: (1) direct synthesis of androgens or estrogens by the tumors; (2) synthesis of gonadotropins that stimulate secondary secretion of androgens or estrogens; (3) alteration of the peripheral metabolism of androgens. In this manuscript we have discussed these mechanisms in relationship to steroid-secreting tumors and gonadotrepin-secreting tumors. In postpubertal males, the most common manifestation of these tumors is the production of gynecomastia. Because the breast is a target organ for the action of both androgens and estrogens, gynecomastia occurs when there is an alterartion in the normal androgen/estrogen ratio. Consequently, gynecomastia may be produced by either deficient androgen production or excessive estrogen secretion. The endocrinology of testicular tumors is a fascinating subject and recent development of sensitiv specific radioimmunoassays for the measurement of tumor markers has opened a new area of investigation into the biology of these neoplasms.


Follow-up of prostate carcinoma with serial bone scanning using cyclotron-produced 18-fluorine. Seventy-four patients with carcinoma of the prostate were studied annually by combined radiological and 18F scintigraphy over a 5-year period. Of the patients who have no radiological evidence of bone metastases, 25% have a positive 18F bone scan. Follow-up of these patients has shown that scan abnormalities perceded x-ray changes from between 1 to 4 years. False negative scans were not seen with 18F which allows for greater accuracy in the detection of skeletal metastases. Teh accurate staging of carcinoma of the prostate cannot be made without bone scanning. Preliminary results with 111In bleomycin as an adjunct to 18F have shown this to be a useful radio-pharmaceutical to distinguish metastases from benign lesions, and further studies are warranted.


Perinatal mortality in the St. Louis metropolitan area. Statistical data in fetal, neonatal, perinatal, and infant mortality were collected from various sources for the St. Louis metropolitan area (St. Louis City and St. Louis County). The overall perinatal mortality rate of 25.8 for the St. Louis metropolitan area in 1973 compares favorably with the national rate of 25.5 in 1973. The prematurity rate at St. Louis City Hospital (SLCH) is almost three times that of St. Mary's Health Center (SMHC), 12.7 in contrast to 4.8. Both the neonatal and perinatal mortality rates at SLCH are about twice the rate of SMHC, neonatal 19.5 versus 7.4 and perinatal 31.7 in contrast to 19.6. Prematurity and its complications still seem to be the leading cause of neonatal mortality. With modern obstetrical and intensive neonatal care, the survival rates for low birth weight infants has improved markedly. The combined survival rates at SLCH and SMHC, 1972 through 1974 for infants weighing 501-1,000 gms 28 percent; 1,001-1,500 gms, 74 percent; 1,501-2,5000 gms, 95.5 percent; and greater than 2,500 gms. 99.7 percent. Recent studies have shown that the long-term prognosis for these low birth weight infants, in terms of neurological or intellectual sequelae is good. Thus, a more aggressive approach to the management of perinatal problems can be expected to yield excellent results.


[Radiology of portal hypertension (author's transl)]. Description of the applications of radiology to portal hypertension, mainly of the 2 most common conditions, the secondary prehepatic block and the intra-hepatic block in cirrhosis of the liver. In prehepatic block the veins in the splanchnic area which will have to be anastomosed to relieve pressure must be shown. This can be done with direct and indirect spleno-portography and by contrasting the proximal and, possibly, the distal mesenteric veins. For intrahepatic postsinusoidal block of cirrhosis of the liver the portal vein and its major contributaries must be shown. In addition, circulation and pressure in the liver and its surroundings have to be elucidated. For this, catheterisation of the umbilical vein and the veins of the liver are suitable. In addition splenoportography and mesentericography have to be used, when transumbilical portohepatography cannot be done.


Lens cataract formation and reversible alteration in crystallin synthesis in cultured lenses. Embryonic chick lenses developed cortical cataracts and altered their pattern of delta-crystallin synthesis within 3 hours, if cultured without their vitreous body or traumatized with their vitreous body attached. delta-Crystallin reverted to the normal pattern by 24 hours in the cataractous lenses. Thus, biochemical differences that are only observable during the initial stages of cataractogenesis can exist between opaque and normal lenses.


Torulopsis glabrata fungaemia. A report of two cases. Two cases of Torulopsis glabrata fungaemia are presented. The literature on detection of micro-organisms in peripheral blood and on systemic T. glabrata infection is briefly reviewed. Microscopical examination of a buffy coat preparation, a simple and rapid procedure for diagnosing this condition, is described. A scheme of criteria which may be helpful in the diagnosis of clinically significant fungaemia is offered.


[Aetiology and pathogenesis of abomasal displacement (author's transl)]. The incidence of abnormal displacement continues to show a gradual increase. The need for preventive measures has therefore become a pressing matter. Successful prevention of abomasal displacement is based on adequate knowledge of the aetiology and pathogenesis. The present state of affairs is reviewed on the basis of the literature, personal experience and studies. Obviously, a large number of factors may play a role in the pathogenesis of abomassal displacement. Although the knowledge of the aetiology of abomasal displacement is anything but complete, a number of (probably useful) preventive measures are suggested.


[Mange caused by Trixacarus in guinea-pigs (authors transl)]. Six cases of mange in guinea-pigs due to the mite Trixacarus caviae (F ain et al., 1972) are reported. The clinical and histological features are described. In spite of the alarming symptoms occasionally shown by guinea-pigs, the usual antiparasitic therapy was effective. Morphological features by which T. caviae may be differentiated from Sarcoptes scabiei and Notoedres muris, are reviewed.


Ocular manifestations of sickle hemoglobinopathies. The ocular manifestations of sickle cell hemoglobinopathies, while often not severe enough to affect vision, may develop in proliferative stages, resulting in arteriolar-venular anastomoses, neovascular proliferations, vitreous hemorrhage and retinal detachment. The authors review the historical, biochemical, and geographic aspects of the disease, and provide detailed descriptions of findings in theconjunctiva, uvea and fundus, with particular emphasis on the retina. Pathogenesis, histopathologic and clinical appearance, and modes of treatment are discussed.


[Quantitative characteristics of serum antitryptic activity]. The method suggested includes incubation of trypsin with native blood serum and subsequent quantitative determination of trypsin, that did not react with inhibitor, by means of the antitryptic precipitating serum. The method is based on the principle of radial immunodiffusion. In native serum that was not subjected to dilution the antitryptic capacity is 210.0 +/- 27.3 microng/ml in people, 238.7 +/- 33.1 microng/ml in rabbits. A distinct dynamics of changes in the antitryptic capacity of blood serum is observed with experimental hypertrypsinemia.


The use of cloprostenol for the termination of pregnancy and the expulsion of mummified fetus in cattle. Misalliance and mummification are two indications for treatment with cloprostenol. Of 56 animals mated accidentally that were treated with 0-5 mg cloprostenol, 12 were more than 150 days pregnant and of those, nine required more than one treatment. The animals treated early in pregnancy aborted promptly and completely whereas two, considered to be 200 days pregnant at the first treatment, failed to respond at all. There were no reports of retained fetal membranes or any adverse side effects. Eight cases of fetal mummification were also submitted for treatment. All aborted within three to five days, the fetus being removed manually per vaginam in each case. Serial blood progesterone assays indicated that successful treatment was associated with leutolysis in all cases. It was concluded that cloprostenol may successfully be used for the treatment of misalliance and mummification of the fetus.


[Cytospectrophotometric study of the concentration of RNA and SH-groups in swine embryos]. Cytospectrophotometry was used to study the contents of RNA and SH-groups in zygotes as well as in 8- and 12-day embryos embryos of the pig large white breed. Significant correlation is found between the content of RNA in the embryos and their survival level in sows. A high degree of variability within one generation is established for the indexes under study. The data obbtained give reasons for a conclusion on the important role of RNA and SH-groups, as their content determines to a considerable extent the biological quality of the embryos and thus their survival rate too.


Germ cell tumors of the testes.

Germ cell tumors of the testes. Most malignant testicular neoplasms are of germ cell origin. They are divided into five basic types: seminomas, embryonal carcinomas, teratocarcinomas, adult teratomas and choriocarcinomas. Clinically they may present as an enlarging testicular mass, or with symptoms resulting from metastases or hormonal secretions. The treatment of choice for patients with seminomas is orchiectomy, followed by radiation therapy. This combination results in an 80 to 100 percent five-year survival rate in patients with nonmetastatic or locally metastatic disease. The treatment of nonseminomatous germ cell tumors is more controversial. An aggressive approach, however, with retroperitoneal lymph node dissection and adjuvant chemotherapy has resulted in an overall 78 percent survival rate. Several placental and fetal proteins are secreted by these tumors. Two of these, human chorionic gonadotropin and alpha-fetoprotein, have been shown to be useful for the diagnosis of these neoplasms, for following the disease activity during therapy and for detection of recurrences.


Persistent measles virus infection in a calf kidney cell line. Note I. Establishment of calf kidney cell lines chronically infected with measles virus and some of their biological characteristics. Several types of persistent measles virus infection resulting in lytic infections could be established in a calf kidney cell line, according to the passage level of the substrate and to the virus strain inoculated. Persistent measles virus infection in calf kidney cells was characterized by the limited proportion of infected cells, the large amount of infectant virus, and the alteration of morphological and growth characteristics of the cells. The mechanism of this persistent infection is discussed.


[Cortical influences on thyroid and sex gland function in neurotic dogs]. Disturbances of higher nervous activity charactetized by the development of an experimental neurosis were elicited in three male dogs by means of functional influences. This was accompanied by an increase, decrease or no change in the level of total thyroxine in the blood with greater fluctuations of its content on different days. An euthyreoid state persisted in the dogs throughout all periods of the neurosis, which is testified by the absence of changes of the free thyroxine coefficient. In the first three to four weeks of the experimental neurosis the testosterone content in the blood plasma was enhanced, its level not correlating with the variations of thyroxine content in the blood.


[Effect of prolonged alcoholization on the cerebral electical activity and emotional behavior of rats]. Two stages of alcohol intoxication were detected in an experiment on 21 rats during formation of addiction to alcohol. It has been shown that the emotiogenic hypothalamic zones may play the part of trigger mechanisms with infolvement of limbic and neocortical apparatuses. At the same time a different type of integration of addiction to alcohol food is possible, in which the emotiogenic zones do not play the principal part. The late stages of formation of addiction to alcohol are characterized by EEG hypersynchronia with a qualitatively different structure in the periods of abstinence and "saturation" with alcohol. A close connection has been recorded between the mechanisms of emotion, hypersynchronia and behavioral epileptic phenomena.


[Dysontogenetic giant cyst].

[Dysontogenetic giant cyst]. Casuistics of congenital dysontogenetic giant cyst which has lead to the displacement and the restriction of the function of the two kidneys. Therapeutic experiment with repeated instillations of formaldehyde for the obliteration of cysts.