Functional properties of the corticotectal projection in the golden hamster.

Approximately 31% of the cells recorded in the hamster's superior colliculus could be activated by stimulation of the ipsilateral primary visual cortex. While cortically activated cells were encountered in all laminae of the colliculus where visual cells were isolated, the highest probability of driving visual cells was observed in the deeper laminae, that is, those ventral to the stratum opticum. Response latency, jitter (latency variability), latency shifts as a function of shock intensity, thresholds, and spike numbers did not vary as a function of depth in the colliculus. There was a clear correspondence between the visual fields of the best cortical stimulus points and the receptive fields of cortically activated cells recorded in the superficial laminae of the colliculus. However, there was considerably less retinotopic fidelity for the cortical areas from which cells isolated in the deeper laminae could be driven. This suggests a greater degree of convergence from relatively widespread cortical regions upon visual cells of the deeper laminae. The visal organization) of the cortically activated cells did not differ appreciably from the overall sample of visual cells recorded in the colliculus. Only 3 of the 159 cells tested were driven by stimulation of the contralateral visual cortex and two of these were responsive only at very long latencies.


Ionic milieu of bovine and ovine rumen as affected by diet.

Postprandial changes in osmolality, mineral, and volatile fatty acid concentrations in rumen fluid were examined in three cattle fed six widely differing diets. Mineral concentrations varied with diet and postprandial time. However, net changes in postprandial mineral concentrations did not contribute significantly to changes in osmotic pressure. There was an effect of diet and time after feeding on concentrations of volatile fatty acids. Postprandial changes in osmotic pressure were primarily due to changes in concentrations of volatile fatty acids. Magnesium oxide and sodium bicarbonate were fed to sheep to increase the osmotic pressure in rumen fluid. The salt supplement decreased acetate, propionate, heat, and methane production. However, rumen water flux was unaffected by the salt supplementation. Diets which produce hypertonic rumen fluid due to high mineral and/or concentrations of volatile fatty acids may reduce fermentation in rumen.


Mastitis infection from abrupt loss of milking vacuum.

During a single reversal trial of two 2-wk experimental periods, teats of all glands of 12 Holstein cows were subjected to a milking routine conducive to large vacuum fluctuations and flooded teat cups. High pathogen contamination was produced by continuous infusion into the long milk tube of 10(10) colony-forming units of Streptococcus agalactiae per min. At each milking, teat cups of control glands (two per cow) were clamped at the short milk tube and removed after milk ceased flowing. As inflations were opening, we subjected teats of the remaining (treated) pair of glands to abrupt vacuum drop by venting the long milk tube at its connection to the bucket of the milking machine. Excluding glands infected at starts of periods, 10 (22%) of 45 treated glands versus 2 (5%) of 43 control glands became infected with Streptococcus agalactiae (criterion: Wisconsin mastitis test rise plus positive culture at two consecutive milkings). We concluded that the rapid airflow toward teats which resulted from sudden vacuum loss characterizes vacuum abnormalities associated with increased risk of mastitis infection.


Limiting amino acids for milk protein synthesis by bovine mammary cells in culture.

Experiments are presented to determine the effect of labeled precursor amino acid on synthesis of beta-lactoglobulin and beta-casein and to identify the most limiting amino acids in Eagle's minimal essential medium for synthesis of milk protein. Protein synthesis was measured by incorporation of carbon-14 labeled amino acids into milk protein from bovine mammary cell culture. Mammary tissue from 13 Holsteins (12.7 kg milk/day) was dispersed and cultured for 18 h. In Experiment 1, three replications (cows) with three amino acid mixtures, utilizing labeled lysine or leucine as the marker amino acid, showed that labeled lysine overestimated the effect of medium lysine, and labeled leucine overstimated the effect of leucine on beta-lactoglobulin and beta-casein synthesis. Subsequently Experiment 2 utilized 10 replications (cows) to determine the effect of a three-fold increase of each of 13 amino acids in Eagle's medium. Increased threonine or methionine improved beta-lactoglobulin synthesis and cystine increased beta-casein. Second and third best response in beta-casein were from addition of methionine and threonine. Combined analysis from 10 cows and two protein responses by multivariate procedures identified, in order of decreasing magnitude, cystine, threonine, and methionine as limiting amino acids in the dispersed cell culture.


Selective absorption of immunoglobulin IgM in the newborn calf.

The efficiency of absorption of colostral immunoglobulin classes was determined by the ratio of the quantity of immunoglobulins in the calf serum after absorption was complete to the quantity in the colostrum fed the calves. The experiment with 58 pooled colostrums assayed for absorbability of immunoglobulins had three to eight calves per assay. Colostrums with similar concentrations of immunoglobulins varied from 10 to 46% in absorption of IgG and 5 to 50% in IgA. The percent of immunoglobulin IgM absorbed increased as the amount ingested decreased. The absorption efficiencies of immunoglobulins IgA and IgG did not change as intake varied. Correlations of colostral immunoglobulins ingested with percent absorption in summaries of two experiments were -.76 and -.90 for IgM, 0 and -.33 for IgG, and -.05 and -.02 for IgA. The selective transport of IgM is important for its role as the primary immunoglobulin giving the calf immune protection during the first few days of life. The variation in absorption of IgM in different colostrums of similar immunoglobulin content was not different from that among calves receiving the same colostrum.


Development of the conceptus in the bovine.

Growth of fetuses, fetal membranes, and fetal fluids was characterized by least squares analysis for 254 conceptuses ranging from 25 to 278 days of gestation. Prior to 100 days of gestation, increases in allantoic fluid volume and chorio-allantoic membrane weight preceded those of fetal weight, amnio-allantoic membrane weight, and amniotic fluid volume. The relative rate of growth of components suggests a prerequisite of chorio-allantoic membrane expansion for subsequent fetal growth. Male fetuses were heavier than female fetuses as early as 100 days of gestation. Rate of growth of all fetuses was maximal at 230 days of gestation with a peak rate of greater than 200 g/day. Growth rate then declined to less than 100 g/day by term.


Cutaneous pseudolymphoma.

The diagnosis of cutaneous lymphoreticular infiltrative lesions is difficult. There are many diagnoses possible, and oftentimes it is difficult to distinguish between a malignant lymphoma cutis and any of the many benign lymphomas. This article deals with a patient who was diagnosed as having metastatic small-cell carcinoma. Because of the inconsistency between the pathologic diagnosis of malignancy and the benign clinical findings and course, a wide ablative excision of the lesion was done and the surgical specimen was studied thoroughly by numerous pathologists. Even then the diagnosis could not be clearly established. The differential diagnosis of cutaneous lymphomatoses is discussed.


Thermal injury on the foot and ankle of a paraplegic: report of a case from exposure to sunshine.

The case of a paraplegic patient who sustained a fullthickness thermal injury to his left foot from exposure to sunshine is described. Electromagnetic energy from the sun can cause enough damage to full thickness of the skin when the heat is intense enough or not dissipated sufficiently. The lesson of it is that paralyzed and anesthetic individuals should be cautious about sunbathing.


Oral squamous-cell carcinoma within a white-sponge nevus.

A 59-year-old woman had thick plaques of leukoplakia on the tongue bilaterally. The condition was initially diagnosed by biopsy as a white-sponge nevus. A second biopsy of a suspicious area on the right side showed squamous-cell carcinoma two years later. It is speculated that prednisone therapy for steroid-dependent intrinsic asthma may have caused a loss of "immunologic surveillance," which permitted development of malignancy in a previously benign condition.


Hypopigmentation at the site of application of a tourniquet.

Hypopigmentation at a site of application of a tourniquet has not to our knowledge been reported in the literature. We are reporting such a case. We have conjectured that this hypopigmentation may be due to temporary anoxia suffered by melanocytes.


Minimum dose requirements of steroid-dependent asthmatic patients for aerosol beclomethasone and oral prednisone.

In 34 steroid-dependent asthma patients who improved markedly during 2 mo of treatment when progressively larger doses of beclomethasone aerosol were added to their oral prednisone regimen, we subsequently reduced both steroids to ascertain the minimum dose of each needed to prevent recurrence of significant asthmatic disability. After 80 wk of follow-up, 15 patients had successfully terminated oral prednisone; 19 were better controlled with a combination of aerosol plus oral steroid than with either drug alone; all patients previously unable to convert to alternate-day prednisone did so successfully during the combined therapy. The minimum effective maintenance dosage varied greatly among these patients-the median values being 2.5 mg prednisone and 1,200 microgram beclomethasone per day. The latter ranged from 200 to 1,8000 microgram. Only 4 patients were satisfactorily controlled without prednisone on 400 microgram beclomethasone per day or less. Seven needed extra intranasal beclomethasone to help control the nasal polyps which worsened after prednisone withdrawal. Suppression of plasma cortisol levels, apparently attributable to the beclomethasone, persisted in most patients, but on the average this was no worse than before commencing this treatment and valuable clinical improvement accrued. There were no other important complications of the regimen. In most of these patients with severe chronic asthma, optimum control of the disease required combined aerosol-oral therapy and maintenance doses of beclomethasone higher than those usually recommended. In some patients, effective control of chronic asthma by beclomethasone treatment may require acceptance of some persisting suppression of adrenal function as a considered risk.


Comparisons of alum-precipitated and unprecipitated aqueous ragweed pollen extracts in the treatment of hay fever.

In 1969 and 1970, groups of patients with ragweed hay fever never before treated were started on preseasonal course of immunization with an alum precipitate of aqueous ragweed extract. A comparison between these two groups of patients and a similar group of patients treated with unprecipitated aqueous extract in 1968 shows that treatment with alum precipitate was safely initiated with fever injections even though a higher dose was administered. The larger cumulative dose appeared to give better IgG antibody responses and greater relief of symptoms. A repeat preseasonal course the next year again required fewer injections of the alum-precipitated extract than a repeat course of aqueous extract.


Antibody deposition in the pleura: a finding in drug-induced lupus.

Pleural tissues from a group of 36 consecutive patients comprised of 15 malignancies, 3 tuberculous, 2 rheumatoid arthritis, 3 procainamide-induced systemic lupus erythematosus (SLE) syndromes, 1 infectious mononucleosis, and 12 nonspecific pleural effusions undergoing needle biopsy were studied by immunofluorescent techniques for antibody deposition. Specific nuclear fluorescence was detected only in procainamide-induced SLE and was characterized by in vivo staining with either IgG, IgM, and in one case, also C3. C1q could not be detected. Two other patients who had antinuclear antibodies (ANA) in their peripheral blood did not have detectable in vivo antinuclear staining in their pleural tissue. The presence of in vivo fixation of ANA in the pleura may be of etiologic and diagnostic significance in procainamide-induced SLE syndrome.


Effect of central air conditioning and meteorologic factors on indoor spore counts.

We have studied the effect of residential central air conditioning on indoor spore counts. The air of 6 pairs of homes (air-conditioned and non-air-conditioned) was sampled volumetrically 4 times daily for 3 consecutive days at 2-hr intervals in the kitchens, bedrooms, and basements with an Anderson sampler. Nearly simultaneous samples were taken outdoors at the same intervals. The total spore count was significantly lower (p less than 0.05) in the kitchens and bedrooms of air-conditined as compared to non-air-conditioned homes. Logarithmic conversion of the total spore count showed significant reduction in all indoor locations of air-conditioned homes. Multiple regression analysis revealed the lower relative humidity of air-conditioned homes to be associated with the lower spore counts. There was no difference in the percent concentration of the major genera within both types of homes. Since reducing the influx of outdoor spores decreases the total spore count without altering the relative concentration of the genera whereas filtration preferentially removes the larger spores, we conclude that the major mechanism in reducing spore counts in air-conditioned homes is the closed windows, although the lower relative humidity and perhaps filtration are also associated with lower spore counts.


Effect of phenobarbital on theophylline disposition.

The effect of chronic phenobarbital administration on the pharmacokinetics of theophylline was studied in 6 healthy, nonsmoking adults. Subject compliance was verified by the determination of plasma phenobarbital levels. Following four weeks of phenobarbital administration, all six subjects showed a resultant increase in serum clearance varying from 11% to 60% with a mean increase of 34% (from 3.01 to 4.04 L/hr/1.73 M2). Theophylline appears to be metabolized more rapidly during chronic phenobarbital administration. This effect, therefore, must be taken into account when administering barbiturates to asthmatic patients for whom theophylline therapy is prescribed.


Prostaglandin-induced eosinopenia in splenectomized rats.

PGE1, PGE2, and PGF2alpha over a dose range of 1 to 50 microgram/kg decreased the number of circulating eosinophils in splenectomized rats. Pretreatment with a beta-blocking agent, propranolol, did not alter this effect, but adrenalectomy eliminated it. Anterior hypothalamic destruction also abolished eosinopenia induced by PGE1 and PGF2alpha but not that induced by PGE2.


Some considerations in the measurement of change.

When conducting a study to assess the "gains in ability" or the "shifts in attitudes" of a group of subjects, the researcher must be aware of the presence of measurement error and regression toward the mean and must attempt to minimize and allow for these phenomena. Also of concern are whether the study can be generalized to a larger population and what statistical techniques are appropriate for the conditions of the study and the type of data collected. Educational and psychologic measurement is subject to particular types of error and must be analyzed and interpreted accordingly. The authors discuss and make recommendations regarding these most important considerations.


Assessing achievement for minimum academic competency. I. Instrument development.

Test instruments for evaluating The American Dietetic Association's Minimum Academic Competencies were developed and tested for four major instructional areas--food, nutrition, nutrition in disease, and management--relevant to professional sciences for general dietetics. The study consisted of four steps: Development of objectives or test criteria; determination of mastery performance standards; test instrument development; and test administration and evaluation. This paper describes procedures used in developing and testing two forms of a criterion-referenced test instrument, which was then administered to determine academic competency of students in dietetics. Salient findings of the test results and evaluation are reported.


Assessing achievement for minimal academic competency. II. Validity and reliability.

Research was undertaken to develop, administer, and evaluate two forms of a criterion-referenced test instrument for measuring the competency status of students graduating from undergraduate programs in general dietetics. Involved in the evaluation of the final forms were 186 students graduating from fourteen college or university programs. Results are reported on the content validity, concurrent validity, reliability, and summary and individual test-item statistics. It is concluded that the test forms need further refinement and development and that the use of criterion-referenced evaluation tools to measure academic competency of students graduating in dietetics is needed.


Assessment of nutrition education of dental students.

This study was undertaken to determine the source and quality of the nutrition education received and to determine the nutritional knowledge gained by dental students. The assessment of the dental education program revealed a limited nutrition component uidely scattered among a number of departments and offered diversely in each of the four years. Faculty interviews and a review of course outlines indicated that some material was presented on foods; disorders; diets; nutrition, biochemistry, and physiology; and anatomical terms. A "self-instructional unit" contained seven tapes on aspects of nutrition. National Dental Board Examinations contained nutrition-related questions which would be helpful in developing a nutrition program, including the development of a dental-related nutritional knowledge test. Such a test, developed within the Department of Nutrition and Food Science, was administered to the dental students on an honor-code basis early in the fall semester. Tests were completed by 178 students. The mean per cent score, 58.7 +/- 10.2, was below the mean for undergraduate students who had completed a basic course in nutrition with a grade of "C". Evidence is presented indicating that the general nutritional knowledge of the dental students increased only during the first year of their program. A gain in nutritional knowledge was noted in the categories of: Nutrition, biochemistry, and physiology; disorders, diets, and organisms.


Sterol content of foods of plant origin.

Available data on phytosterols from the world's literature have been compiled and summarized. There still exists a paucity of data on the quantities of plant sterols in many foods. More extensive data are available on the relative sterol composition. Our compilation shows that plant oils are excellent sources of phytosterols. Nuts and seeds contain moderate levels, and fruits and vegetables generally contain the lowest concentrations of plant sterols. Analyses of the minor sterols, namely, the delta5- and delta7-phytosterols, have become available only recently.


Validity of the 24-hr. dietary recall and seven-day record for group comparisons.

The internal validity of a 24-hr. dietary recall and a seven-day dietary record was investigated among a group of non-institutionalized elderly subjects who were participating in a congregate meals program. Internal validity was assessed by comparing reported intake with unobtrusively obtained data on actual intake. Validity results suggest that the recall is prone to over-reporting low intakes and under-reporting high intakes. This pattern has been referred to as the "flat-slope syndrome." Records collected during the first few dyas were less prone to this syndrome; however, validity declined by the fifth, sixth, and seventh record days. Also, as the record progressed to the seventh day, the demographic nature of the sample became biased due to drop-outs and decreased usability of the records.


New dimensions: the dietitian in private practice.

Private practice is coming to the fore in the profession of dietetics. It can serve as a much needed arm of the health care delivery system in the area of nutrition. Institutions need to re-evaluate and expand their opportunities to sue private practioners, thus releasing more employee time for short-term remedial counseling and education. Referral processes need to be designed and implemented to open new opportunities for consumers to choose and obtain continuing services. Private practice must be included in funding methods, such as third-party payment. Educationl institutions must design programs to prepare persons for private practice. The profession must solve the problem of obtaining community recognition of properly qualified professional authority. The profession must also support the concept of a lifetime career in direct private practice and plan career tracks to keep the most able practioners available to consumers.


Serum uric acid and the renin-angiotensin system in hypertension.

To study whether the renin-angiotensin system is related to hyperuricemia in hypertension, the serum concentration of uric acid was determined in 96 patients with various types of hypertension and various degrees of plasma renin activity (PRA). In malignant hypertension, both PRA and the serum uric acid level were higher than in essential hypertension; but in primary aldosteronism or desoxycorticosterone-excess hypertension, they were lower than in the essential type. In renovascular hypertension, PRA was higher than in essential hypertension, but the serum uric acid levels were similar. There were no differences in PRA and serum uric acid concentration between Cushing's syndrome and essential hypertension. The serum uric acid level in high-renin essential hypertension was higher than in either the normal-renin or the low-renin type. There was a significant correlation between serum uric acid concentration and PRA in the basal state, and between the change in PRA and the change in serum uric acid induced by administration of furosemide. Apparently the close correlation between the renin-angiotensin system and the concentration of serum uric acid is related to changes in extracellular fluid volume, although an intrarenal effect of angiotensin II cannot be excluded.


Relationship of age and biomedical risk factors to progression of coronary artery disease.

The relationship between age, biomedical risk factors and the progression of occlusive disease of the coronary arteries was studied in 176 patients (age range, 27-66 years) who had undergone at least two cine angiograms. The biomedical risk factors of interest were serum concentrations of cholesterol and triglycerides, smoking, hypertension, diabetes mellitus, family history of coronary disease, electrocardiographic abnormalities, obesity, and age. The findings did not reveal any significant differences in mean lipid levels between patients showing progression of disease and those who did not. However, the distribution of serum cholesterol values indicated more hypercholesterolemic patients among the disease-progression group, and more patients with ideal cholesterol levels among the no-progression group. The other biomedical variables did not appear to be related to the progression of coronary disease. Among the older patients, hypercholesterolemia and diabetes mellitus were related to disease progression. Among the younger patients, smoking was related to progression.


Geriatric rigidity and its psychotherapeutic implications.

The elderly tend to be more rigid than younger adults in their attitudes and personalities, and such rigidity is correlated with poor adjustment. However, the rigidity is not the cause of the poor adjustment; rather, it is an attempted solution. One of the patterns of rigidity is an outgrowth of the lifestyle of pessimism, suspicion, self-reliance, self-discipline, determination, and endurance. Two case studies are presented illustrating how rigidity can channel thoughts and prevent the brooding which works itself into depression or anxiety, while simultaneously reinforcing self-help behavior. Geriatric psychotherapy should be problem-centered and should not launch a frontal assault on rigidity or attempt to reconstruct the patient's personality. Behavioral modification, authority, and motivation slogans can be used in conjunction with the rigidity to improve the patient's coping ability.


The evening meal and atherosclerosis.

A trial by any means that can possibly prevent or retard the development of arteriosclerosis is to be recommended. Arteriosclerosis is the chief cause of strokes and heart attacks. The heavy evening meal now in vogue is characteristically rich in animal fats. Normal digestion is at its peak at about the seventh hour, usually during sleep, when the body economy is at its lowest ebb. A large amount of digested material with a high content of lipids is dumped into the slow-moving circulation. As this material, full of saturated fats, moves slowly through the arteries, the situation is ideal for clot formation, possibly resulting in a stroke, a heart attack, or sudden death. Such a catastrophe can occur in almost anyone, but is more apt to strike the high-powered executive or the apparently healthy man past 45 who has a voracious appetite. It seems logical to postulate that a light, rather than a heavy evening meal would result in less arteriosclerosis with complications such as heart attacks and strokes.


Cardiovascular changes in/of old age.

Although the causes of aging are still unknown, the effects of decrements in anatomic structure and physiologic function have led to wrong concepts and resulting discriminatory policies against old people. Cardiovascular changes are common among the aged but are not necessarily symptomatic; they may be the result of past illnesses or signs of diminished cardiac reserve. In many cases, a cardiac abnormality detected by our modern diagnostic hardware dose not in itself constitute the necessity for treatment. Illustrative cases are cited. A rise in blood pressure with advanced age often is a sign of adaptation to the increased rigidity of the arterial system. Certain heart murmurs mimicking mitral insufficiency may indicate slight papillary muscle dysfunction or a clinically insignificant degree of mitral valve prolapse. On the other hand, the changing clinical status in old age may involve a diminution of symptoms. Therefore, in geriatric medicine, the physician should be on the alert for aberrant manifestations, e.g., painless myocardial infarction or atypical pulmonary embolism. Psychologic evaluation is important. There is no cardiac disorder which is typical for the older age group, but also there is none from which older people are exempt.


Sinus bradycardia in the aged.

In the aged, sinus bradycardia represents the sinus-node-dysfunction component of diffuse conduction system disease associated with coronary and cerebral arteriosclerosis. Findings are presented on 15 patients whose ages ranged from 69 to 93 years at the time of admission to a home for the aged. They remained under observation (with electrocardiographic data) for from less than 1 year up to 18 years. The findings illustrate the evolution of conduction system abnormalities, including sinus node dysfunction, occurring either as an initial or an interval event. These abnormalities represent positive indications for pacemaker insertion in the aged.


Hyaluronuria in a case of progeria. (Hutchinson-Gilford syndrome).

A classic case of progeria (Hutchinson-Gilford syndrome) in a 9-year-old Japanese boy is presented. The characteristic clinical features in this patient were similar to those reported in the literature. The total amount of acid glycosaminoglycans excreted in the urine was within the normal range, but there was an increase in hyaluronic acid excretion. The hyaluronuria was a novel finding in progeria, providing a common linkage with the hyaluronuria found in Werner's syndrome.


Depression in the elderly. II. Possible drug etiologies; differential diagnostic criteria.

Drugs for the treatment of various medical or psychiatric disorders may sometimes cause or exacerbate depression in the elderly. Drug interactions due to polypharmacy (a common method of prescribing for aged patients) may also play a role the genesis of depression. The roles of specific drugs and of drug interactions are reviewed. Criteria for the diagnosis of depression in the elderly, as well as differential diagnostic guidelines, are presented and constructive suggestions offered.


Nutritional hypovitaminosis-D and the genesis of osteomalacia in the elderly.

A review is presented of the genesis of osteomalacia due to dietary lack of vitamin D in the elderly in the United Kingdom. Subjects at high risk are identified and the diagnostic difficulties discussed. The importance of dietary vitamin D and exposure to sunlight in relation to vitamin status is stressed. The role of vitamin D in calcium metabolism is now much more clearly defined; the final active form of the vitamin is 1,25-dihydroxy-vitamin D, which acts like a hormone after the vitamin molecule has undergone two chemical modifications in the liver and kidney. A correlation between lack of vitamin D and femoral neck fractures has been established. Six illustrative cases of osteomalacia in the elderly due to dietary lack of vitamin D are reported.


Our elderly. Who are they?

Why are today's elderly remarkable? They are the small minority who represent the survivors of Americans and immigrants born during the period 1870-1913. The mortality experience during that period shows the dramatic changes that have occurred. The future populations of the elderly are developing with an ever-increasing number of people kept alive by the mass use of vaccines, the application of new medical, surgical and psychologic diagnoses and therapies, effective antibiotics, rapid communication, proper nutrition, safe water, a high level of health insurance coverage, and available education at all levels for those who want to participate.


Young adult, geriatric and aphasic group responses to simple analogies.

The efficiency of interactive language processing was compared in three groups: 25 young adults, 25 geriatric subjects, and 25 adult aphasic subjects. The task involved simple analogy completion. Data analysis included six categories of responses which represetned levels of convergence toward the expected relationships. Results of chi-square comparisons between groups were all significant, and indicated substantial reductions in interactive processing for geriatric subjects. The findings suggest that a general aging factor may contribute to the poor prognosis in elderly aphasic patients.


Pharmacy services in a Federal extended care facility, as provided by a pharmacy student.

The objective of this study was to identify the role of the pharmacy student in providing health care to a Federal Nursing Home Care Unit. Under the supervision of a graduate pharmacy resident, the student was responsible for monitoring compliance in drug therapy, providing consultation services, and developing several research projects. A detailed study of allergy documentation in 78 patients showed that 25.6 percent of their records indicated drug allergies, but upon further investigation only 5.1 percent could be confirmed. Fourteen (41 percent) of the 34 reported allergy-producing substances had been administered again after the initial allergic incident, without ensuing difficulties. Evaluation of the self-medication program indicated that 71.4 percent of 14 study patients could not easily open their child-proof medicine container. A change to "tight" containers enhanced the patients' ability to open them. However, no significant conclusions could be drawn regarding improvement in the patients' compliance with prescription directions. Pharmacy students can be a valuable resource in improving documentation, testing drug prescription compliance, and participating in the general care of patients in long-term facilities.


Bamboo phobia in an eighteen-month-old boy.

We have presented a developmental history and an account of the treatment of an eighteen-month-old boy who had a bamboo phobia. Conceptual problems concerning drive organization, the expression of drive derivatives, and object relations have been explored. The correlation of these with Mahler's separation-individuation theory has been considered. Analysis of the bamboo phobia revealed two castration themes. One related to projections onto the father, and another onto the mother and sister. Our case illustration highlights the important role of object-relations development in determining the level of psychosexual organization. It also demonstrates the difficulty in correlating level of psychosexual development with capacity for symptom formation. The importance of sexual and aggressive impulses in separation-individuation is emphasized. The bamboo phobia is seen as a symptom formed to deal with psychosexual conflicts that played a part in the rapprochement crisis. Tth role of fantasies and internal representations of objects, as well as the parents' actual behavior, is stressed--again, in relation to the influence of the drives.


The role of audition in early psychic development, with special reference to the use of the pull-toy in the separation-individuation phase.

The role of audition as an important perceptual modality in early psychic development has been neglected. Some reasons for this neglect are suggested. In the development of psychoanalytic technique, the analyst has changed from a "tactile presence" to a "visual presence," then finally, with the analyst positioning himself behind the couch, to an "auditory presence." Several clinical examples from analytic patients as well as child development in normal and deaf children provide instances of each type of perceptual "presence." It is suggested that, in evaluating analyzability, analysis requires a specific ego ability, namely, tolerance for the analyst as an "auditory presence." It is emphasized that some patients, for reasons of development, constitution, and/or significant stress (separation), cannot work with the analyst as an "auditory presence," but regress to the analyst as a "visual" or "tactile" presence. The importance of audition in early mother/stranger differentiations, and in the peek-a-boo game, is a developmental precursor to the use of audition as a contact modality in the separation and individuation phase. Audition permits active locomotion and separation from tactile and visual contact modalities between toddler and mother, while at the same time maintaining contact via their respective "auditory presence" for each other. The utilization of the pull-toy in mastering the conflicts of the separation-individuation phase is demonstrated. The pull-toy is heir to the teddy bear and ancestor to the tricycle. Greater attentiveness to the auditory perceptual modality may help us understand developmental phenomenon, better evaluate the potential analysand, and clarify clinical problems of audition occurring in dreams and those areas of psychopathology having to do with auditory phenomena. The more refined tripartite conept of "presence" as it relates to the predominant perceptual modality--tactile, visual, auditory--is felt to be a useful conceptualization for both developmental and clinical understanding.


The testing of reality from the standpoint of the body self.

Traditionally, the body self has been regarded as involved in reality testing principally in the determination of the distinction between the source of a stimulus being internal or external. The body has been closely related to instinctual drives, to urgency, and thus to conflicts that are crucial to normal and pathological development. However, the concept of the body self comprising all the psychic experiencing of body sensation, body functioning, and body image is broader than the body's connection to inner-outer reality testing and id pressures. The findings from childhood observations and from clinical research indicate that at each stage of the child's life, from earliest infancy on, the maturation and development of the body self becomes integrated with the whole group of psychic regulatory activities that contribute to the individual's sense of reality. The development of the body self at each period involves criteria by which some aspect of reality is tested. The subject of this paper has been the developmental pattern of experiences that contribute to the individual's sense of his body in particular and to the sense of reality of the self in general.


The teaching and learning of psychoanalytic developmental psychology.

I have described three areas in psychoanalytic education where I believe greater attention to existing methods is necessary so as to facilitate the learning of certain principles of development. Explanations might be enhanced by including constructivist with reductionist elements; metapsychology might be clarified in a way that would make the "developmental" more available to analytic scaffolding; and finally, the technical precepts that help focus on the emergence of regressive trends should be broadened to permit enhanced awareness of the progressive trends as well.


Candidates' evaluation of psychoanalytic supervision.

The origins, methods, results, and discussions of a project undertaken by the advanced candidates at the Denver Institute for Psychoanalysis have been described. This project was designed to examine the supervisory process and to compile a consensual critique of individual supervisors from the candidate's point of view. We have included vignettes of supervisory experiences. It became our purpose to: (1) Develop a set of criteria by which the strengths and weaknesses of supervisors can be evaluated, (2) Involve the candidate in assessing his own learning needs, (3) Promote the objective assignment of supervisors, an assignment determined by the patient's problems, the candidate's learning needs, and the supervisor's varying capacities and abilities to effectively understand these problems and deal with these needs, (4) Expand the supervisory process to include what we felt were often unconscious or hiterto unacceptable issues, such as the function and use of countertransference, and the detection and open discussion of transference dilemmas between analyst and both patient and supervisor, (5) Stimulate dialogue at all levels of psychoanalytic education on the patient-analyst-supervisor relational system.


[Arterial vascularization of the optic nerve trunk in humans (author's transl)].

We study the arterial vascularization of the trunk of the optic nerve partialarly in its anterior part in human. We injected in the carotids, a mass of colored an radio opaque substance, in the whole cadaver that allows simultaneously a visual study and a radiologic control of the results. This one is completely insufficient to allow a study of details; so we used the diaphanisation as described by Spalteholz; we were able to confirm Hayreh's results: the ophthalmic artery has a variable pathway according to individus but overcrosses the most frequently the optic nerve. The posterior ciliary arteries have also an origin pathway very variable; the posterior external ciliary is the most commonly present. The central retinal artery takes its origin the ophthalmic artery no more than half of the cases, and elsewhere from a common trunk with the ciliary arteries, or even from branches of those last arteries. It always penetrates the nerve in its inferior face and is responsable of its vascularization in periphery by the man of its pial branches and in depth by its intra neural branches.


[The child's vision and its measurement. Comparative results of different tests of visual acuity in children as a function of age].

The authors describe a new test for determining the visual acuity in pre-school children. It is a picture-test which is composed by pictures of animals of varying sizes, the child being asked to name them in turn. The six pictures (duck, rabbit, cock, elephant, fish, and butterfly) with an homogeneous density, can be utilized according to several manners-appariement or verbal expression--and several distances: 2,50 m or 5 m. That test gave good results on children aged from three years upwards, however the performances could be increased by prectising exercises.


[Morphological remarks concerning the venous circulation of the uveal tract (author's transl)].

The author has undertaken a study of the means of drainage of blood from the chorio-capillary system. The veinules arise in three essential ways: "comb teeth", "palm" and "vortex". Study of the prevortical veins showed, in addition, the presence of numerous fine anastomotic vessels which unite the veins which run to the vortical veins.


[Prevention of ocular toxicity of ethambutol: study of zincaemia and chromatic analysis (author's transl)].

In order to define the means of prevention of the ocular toxicity of ethambutol (EMB), 72 patients under treatment with EMB-INH-Rifampicin combination were subjected to systematic analysis of plasma, blood and urinary zinc levels together with a full ophthalmic examination. Comparison of the results from 12 patients sustaining ocular damage at the time of treatment with the results of 60 others: 1) demonstrates the existence of a correlation between plasma zinc levels before treatment and the probability of ocular damage from EMB (high risk for zinc is less than 0.70 mg/l - almost no risk for zinc greater than 1 mg/l); 2) confirms that dyschromatopsia is one of the first signs of ocular damage through EMB and thus constitutes an alarm signal; 3) suggests that the ocular toxicity of EMB might appear in the retina before the optic nerve.


[The diagnosis of isolated choroidal hemangioma (author's transl)].

The authors report clinical and laboratory data setting the diagnosis of isolated choroidal hemangioma. Main elements appear to be biomicroscopy and angiography after fluorescein and cardiogreen injection. The clinical history of two non typical cases is reported and discussed.


[Atenolol: its effects on pressure in open-angle glaucoma].

1. Instillation of 2 drops of 4% solution of atenolol decreases the ocular pressure in open angle glaucoma. 2. In 12 glaucomatous patients treated as in-patients, 4 daily instillations of 4% solution lower the average tension of the group below 20-22 mm Hg day and night. 3. The tensionnal effects of 4 or 6 daily instillations on out-patients seems to be less effective. 4. The hypotensive effects of atenolol, on some of the patients at least, decreases during the second month of uninterrupted treatment. However, the response to a single dose remains constant even after a long term treatment. 5. Treatment with atenolol is remarkably well tolerated. It does not provoke either local or general side effect. 6. Although the combination of atenolol and myotics or carbonic anhydrase inhibitors produces an additive pressure lowering effect this results in a loss of the advantages of atenolol with regard to absence of undesirable pharmacological effects.


[Antiglaucomatous therapy with reduced concentrations (author's transl)].

By reducing the concentration of hypotensive drops and increasing the number of instillations, a therapeutic effect similar to that of the concentrated drop can be obtained, with a reduction of side-effects. The results of experiments with pilocarpine, adrenaline-guanethidine combination and propranolol are described.


[Mucous graft in acute spastic ectropion (author's transl)].

Acute spactic ectropion often occurs with concomitant inflammatory and degenerative conditions of the conjunctiva. This is the cause of failures in the classic ectropion's surgery. Authors suggest to replace the altered conjunctiva with transplant of oral mucous membrane.


[The new surgery of functional esotropias (author's transl)].

In two previous papers published in this journal, mechanic phenomena induced by the surgical procedures and the surgical management of fundamental troubles in esotropias were analyzed fully into details. In this third part, the authors explain the various pratical conclusions which result from their study. They underline that more than 60-70% convergent squints in children have a apastic component, and to overcome it classical procedures alone (resections and recessions) are fully unfitted. Equally, the Fadenoperation cannot by itself make away with motor disorders. Generally, the various technics must be associated to get cure. But to draw up an adequate surgical plan it is necessary to take into account the anaesthetic sign and the muscular traction test. The Authors have settled a pratical surgical scale allowing to know immediatly according to clinical and electro-oculographic findings, and the results to those tests, the particular operating actions which are convenient to do in each case and also their amount.


[Corrective methods of the aphaque eye (author's transl)].

While in most cases, the operation for cataract gives full satisfaction to the patient, it is nevertheless a fact that in some cases--perhaps only a few but certainly not to be neglected--the long term effects of such an operation are not well tolerated. The cause is not faculty performance of the operation, but most often unsuitable operational timing or technics or else defective or illchosen visual aids. These, therefore are the problems dealt with in this paper by the authors who made thorough study of the aphaque eye and its corrective methods.


[Discussion of terminology concerning disorders of the optic nerve and disc (author's transl)].

Progress over the past few years in knowledge concerning disorders of the optic nerve and disc leads to changes in their classification. A new terminology is proposed. It uses a minimum of terms, their very general sense being understandable by any nonophthalmologist physician. It is based upon clinical symptoms and signs, thus being immediately applicable in clinical practice.


Limited exposure of rats to H(2)SO(4) with and without O3.

The purpose of this study was to determine the effects of sulfuric acid (H(2)SO(4)) alone and with ozone (O(3)) on rats. To accomplish this, rats were exposed for 8 hours daily to an atmosphere containing either nebulized H(2)SO(4), H(2)SO(4) plus 0.9 ppm O(3), or 0.9 ppm O(3). The atmosphere in the exposure chambers was maintained at a fairly constant temperature and humidity. Nebulized H(2)SO(4) was delivered uniformly to provide a particle size of about 0.3 micron mass median diameter (MMD) and a mass concentration of 2 mg/m(3). In preliminary experiments, animals exposed to 2 mg/m(3) of H(2)SO(4) daily for 82 days showed very slight morphologic injury to the respiratory tract. In contrast, biological effects were readily demonstrable in rats exposed to H(2)SO(4) plus O(3) or to O(3) alone, possibly with some enhancement of effect in animals exposed to the mixture. The effects observed were characteristic of the response to O(3) alone.


Exchange transfusion treatment of methylmercury-poisoned children.

Three children, ages 6 months, 13 months and 10 years were treated by exchange transfusions following exposure to methylmercury in the Iraq outbreak in 1971-72. Two had severe signs of poisoning and the other was clinically normal but had a high blood mercury concentration. Exchange transfusion resulted in clearance of mercury not only from blood but also from other tissues. The average amount of mercury removed in a two hour exchange was estimated as 6% of the body burden as compared to 1% of the body burden removed by normal excretion processes in 24 hours. No dramatic improvement in the clinical condition of the two poisoned patients occurred. Some improvement in motor power and function was observed during follow-up visits to their houses.


Red cell damage induced by peroxidized microsomes: the relationship between hemolytic activity and peroxide content.

Rat red blood cells will hemolyze if they are present in vitro in mixtures of rat liver microsomes in which lipid peroxidation has been initiated by NADPH. Recent work from this laboratory indicated that a toxic factor not having radical properties could be generated from the lipids of the peroxidizing microsomes. This toxic factor produced prelytic damage in rat red blood cells. In this communication we show that if Ca(++)-aggregated microsomes are first peroxidized and then sedimented by centrifugation, the resuspended peroxidized microsomes are capable of hemolyzing red cells in the absence of any further microsomal lipid peroxidation. This result shows conclusively that the microsomal lipid peroxidation step can be separated from the attack on red cells leading to frank hemolysis. Furthermore, lipids extracted from the peroxidized microsomes with chloroform-methanol account quantitatively for the degree of hemolysis produced. The active hemolytic material could not be detected in resuspended microsomal centrifugates obtained during the first 10 minutes of NADPH-stimulated microsomal lipid peroxidation. It appeared rapidly after 10 minutes. It was maximal at 20 minutes, and fell to a low level of activity by 60 minutes. Peak hemolytic activity correlated with peak generation of lipid soluble peroxides. High, but less than maximal levels of peroxides appearing at 10 minutes did not cause hemolysis, and high, but less than maximal levels remaining at 60 minutes were only weakly hemolytic. The extracted lipoidal material with hemolytic potency is more reactive than hydrogen peroxide in a peroxide assay.


The determination of moments for extension of the wrist generated by muscles of the forearm.

In deciding on suitable tendon transfers to replace denervated muscle-tendon units, important considerations are the strength and effectiveness of possible substitutes. A method is presented by which the strength of the wrist extensor muscles and their moment arms can be determined. The method can be applied to other muscles at other joints. It involves the use of a force transducer which measures the combined forces of the three wrist extensors in an isometric contraction. This moment for wrist extension, measured in the living intact arm, is the same as the sum of the moments of the three wrist extensor muscles. The contribution of each muscle to the total moment is calculated from ratios that have been developed from a quantitative study of moment arms and muscle masses in sixteen cadaver limbs. It is suggested that the ratio of one moment arm to another is fairly constant from subject to subject, and that muscle masses also have sufficiently similar ratios to each other to serve as the basis for practical estimations by the surgeon. Thus the surgeon needs only one or two direct measurements of moments externally and only one or two skeletal measurements on any living subject to be able to estimate the effectiveness of a number of muscles on the basis of cadaver studies such as this, and to project the behavior of a muscle after it has been transferred to a position where it will have new moment arms.


Von Frey's method of measuring pressure sensibility in the hand: an engineering analysis of the Weinstein-Semmes pressure aesthesiometer.

The development of microsurgical techniques has generated a resurgence of interest in estimating local pressure sensibility as a measure of sensory improvement. Because our experience with Weinstein's modification of Von Frey's probes yielded variable and poorly understood results, we measured two sets of probes and examined them in the light of the engineering principles on which their behavior is based. The mechanical behavior of the nylon monofilaments can be described as buckling with one end built in and the other end pinned. The probes are relatively uniform and consistent. However, no loss in sensitivity would accompany division of the set into two or three equivalent sets. Variations in the buckling stress as high as a factor of eight are difficult to avoid. Gross errors arise from careless application, variations in the elastic modulus due to changes in temperature and humidity, and variations in the attachment of fibers to handles and differences in the ends of the filaments. Interpreting results for this instrument requires an understanding of the factors which can influence those results. The rpobes are simple to use but easy to misinterpret.


Scaphotrapezial trapezoidal arthrosis.

Scaphotrapezial trapezoidal degenerative arthritis as an isolated entity or as a preponderant part of a pantrapezial degenerative process was seen in 49 hands of 34 patients. Pain and weakness were the primary complaints. Point tenderness was present in all but two hands. Significant loss of grasp and pinch strength was noted in 18 hands, and loss of wrist motion occurred in 16 hands. Roentgenographic findings included narrowing of the scaphotrapezial trapezoidal joint space, subarticular cortical sclerosis, and cyst formation. A dorsiflexed position of the scaphoid, with a scapholunate angle of less than 45 degrees, was noted in 38 of the 45 hands. Late collapse of the intercarpal joint with dorsiflexion instability of the lunate also was noted. Twenty-nine hands were treated conservatively by protective splints, corticosteroid injections, and avoidance of exacerbating activities. Surgical treatments consisted of fibrous arthroplasty (four), silicone interposition arthroplasty (six), trapezial replacement arthroplasty (three), arthrodesis (five), joint debridement (one), and trapezial excision (one). Late results were more encouraging than were early results and were moderately satisfactory for each method. Improvement in grasp and pinch strength was most predictable for arthrodesis, although wrist motion was diminished.


Treatment of trapeziometacarpal arthritis: results of resection arthroplasty.

Ninety-one thumbs with symptomatic trapeziometacarpal arthritis were studied and classified by the severity of trapeziometacarpal arthritis, as seen on the roentgenogram. Thirty-five patients with mild changes had satisfactory relief of pain after treatment with anti-inflammatory drugs and a C-splint. Operation, consisting of resection of the trapezium and shortening by 1.5 cm of the abductor pollicis longus tendon was performed on 16 thumbs. In six patients a rolled palmaris longus tendon was inserted between the metacarpal base and the scaphoid. All patients had relief of pain, improvement of pinch and grip strength, and an increased range of thumb pronation after operation. There was no difference in the results of those patients treated with or without tendon interposition. Trapezial resection was found to be an effective means of treating patients with symptomatic trapeziometacarpal arthritis who failed to improve with conservative care.


Hybridization between different strains of Schistosoma intercalatum Fisher, 1934 from Cameroun and Zaïre.

The offspring from hybridization experiments between Schistosoma intercalatum, Cameroun (intermediate host Bulinus forskalii) and S. intercalatum, Zaïre (intermediate host B. globosus) was investigated. Reciprocal crosses between the two forms were performed. F1- and F2-miracidia developed in both parental intermediate hosts. The minimum prepatent period was 24 days at 26 degrees C, an average of the prepatent periods for the two parental strains. The total cercariae production per 100 exposed snails decreased sharply from 121 000 for the parental strain from Cameroun in B. forskalii to 21 000 and 5000 from the F1- and F2-generations, respectively. The figures for S. intercalatum, Zaïre in B. globosus were 270 000, 134 000 and 3000, respectively. F1- and F2-generations were from the cross between males from Cameroun and females from Zaïre. The viablility of the four cross-breeding experiments carried out (two with each combinations of the parental strains) were low. It was only possible to obtain F2-cercariae. No miracidia were hatched from eggs produced by the F2-gerneation.


Egg-laying and hatching rhythms in the monogenean Diplozoon homoion gracile from the southern barbel (Barbus meridionalis).

Diplozoon homoion gracile from the southern barbel (Barbus meridionalis) displays an egg-laying and an egg hatching rhythm, the latter being more clearly defined. Egg laying continues both day and night but significantly more eggs are laid at night. The oncomiracidia hatch at dusk. If parasite eggs are eaten by the host they pass through the gut undamaged and hatch normally.


Allometric growth in three species of digenetic trematodes of marine fishes from Belize.

Allometric growth of body proportions and organs is determined for three speciies of digenetic trematodes of marine fishes from Belize: Apocreadium mexicanum Manter, 1937 (Apocreadiidae), Pseudocreadium lamelliforme (Linton. 1907) Manter, 1946 (Lepocreadiidae), Paracryptogonimus americanus Manter, 1940 (Cryptogonimidae). These are compared with three other species in which allometric growth has been studied. In all species only the hindbody shows positive growth, whereas the forebody, suckers, and pharynx are consistently negative. The body width, posttesticular body, testes, and ovary are positive in some species and negative in others. In some instances the body proportion or organ is growing at the same rate as the body length.


New schistorophine nematodes (Acuraiidae) from New Zealand shore birds.

Viktorocara torea and Sciadiocara tarapunga n. spp. are described from specimens from the gizzards of Haematopus ostralegus finschi and Larus novaehollandiae scopulinus respectively. All hosts were collected in the South Island, New Zealand. V. torea is characterised by the possession of doubly bifid apices to the long spicule and absence of a subterminal spicular spur; presence of six preanal papillae, the first and second widely spaced: the muscular region of the oesophagus constituting about one quarter of the total length of the oesophagus. S. tarapunga is similar to S. umbellifera, but differs in having the muscular region of the oesophagus shorter than the glandular region, a more slender body in both sexes, much smaller spicules (78--91 micrometer and 275--359 micrometer) with the right spicule not more than one-third as long as the left one. The long spicule bears a pair of similar, rounded, laterally placed spatulate apices. It is suggested that Sciadiocara umbellifera of previous authors may encompass more than one species.


Life history of Allocreadium fasciatusi Kakaji, 1969 (Trematoda: Allocreadiidae) from the freshwater fish Aplocheilus melastigma McClelland.

The life history of Allocreadium fasicatusi which occurs in the intestine of a freshwater fish Aplocheilus melastigma has been worked out in detail. The snail Amnicola travancorica acts as the first intermediate host. The mircadidium hatching out from the eggs attacks the snail and passes throught two generations of rediae. Cercariae are of ophthalmoxiphidiocercous type with very long tail and are identical to Cercariae Indicae XLIX Sewell, 1922. The cercariae penetrate and develop into metaceracriae in the haemecoel of the copepods Mesocyclops leuckarti, Microcyclops varicans and Marcocyclops distructus. Upon ingestion by the definitive host, the metacercariae excst and develop into adults. All the stages in the life cycle are described and the life cycle is compared with other allocreadiid life cycles. The original description of A. fasciatusi is revised and Psilostomum chilkai Chatterji, 1956 from Lates calcalifer is synonymized with it.


Immunohistochemical and ultrastructural distribution of antibodies to troponin-C and troponin-I in normal and dystrophic chicken skeletal muscle.

The pectoral muscles from normal and dystrophic chickens were reacted with rabbit antisera to troponin-C and to troponin-I, and the distribution of antibodies was determined by fluorescence microscopy of antibody-stained myofibrils and immuno-electron microscopy of separated I band segments. Chickens of dystrophic strain 308 and control New Hampshire hens were used in this work. Myofibrils which were prepared from both normal and dystrophic muscles and reacted with anti-troponin-I were fluorescent in the I band and A band regions. The Z lines and H zones were unstained. Myofibrils prepared from normal pectoral muscle and treated with anti-troponin-C yielded a pattern of fluorescence similar to that for anti-troponin-I treated myofibrils. However, those myofibrils isolated from dystrophic muscle and reacted with anti-tropinin-C had a weak fluorescence over their entire lengths, and discrete A- and I-band staining was not visible. These results were confirmed by ultrastructural studies of separated I segments reacted with the antisera. It is concluded that in the dystrophic muscle either the antigenic sites of troponin-C are changed which results in a loss of antibody-combining ability or these sites are masked in some way which prevents the reaction with the antibody.


The cytochemical localization of adenylate cyclase: fact or artifact?

In a study of the location of adenylate cyclase activity in rat pancreas with the method of Reik et al. (Science 168:382, 1970), as modified by Howell and Whitfield (J Histochem Cytochem 20:873, 1972) it was found that (a) unspecific staining occurs in rat pancreatic tissue fragments incubated in the Reik-Howell medium in the absence of substrate; (b) addition of adenylyl-imidodiphosphate (AMP-PNP) as substrate, either alone or together with stimulants of rat pancreas adenylate cyclase (secretin. NaF), does not result in increased precipitation; (c) cytochemical incubation of isolated rat pancreatic acinar cells and of rat liver and kidney fragments does not lead to substrate-specific precipitation. In subsequent chemical studies we have found that cyclic adenosine monophosphate (AMP) formation from [alpha32P]AMP-PNP in the presence of rat pancreatic particulate matter is very low in the Reik-Howell medium without lead ions, but is stimulated by addition of lead nitrate (4 mM). Whereas heat-treatment of the particulate matter abolishes all cyclic AMP formation in the absence of lead ions, it actually increases cyclic AMP production in the presence of 4 mM lead nitrate. This indicates that the cyclic AMP formation in the complet Reik-Howell medium occurs by a nonenzymatic mechanism. In addition, this medium shows a tendency to become turbid, particularly when calcium ions are added to the medium, suggesting a possible explanation for the apparently specific cytochemical detection observed by other authors. A revised cytochemical medium, with barium replacing lead and with a pH of 8.9 (optimal for adenylate cyclase with AMP-PNP substrate), leaves rat pancreatic adenylate cyclase activity intact and hormone sensitive, while it is still able to precipitate imidodiphosphate. However, cytochemical incubation of isolated rat pancreatic acinar cells in this revised medium in the presence of AMP-PNP and secretin does not yield an electron-dense precipitate, showing that the enzyme activity is to low to produce sufficient imidodiphosphate. These findings throw further doubt on the validity of the cytochemical detection of adenylate cyclase, reported by other investigators, notwithstanding the alleged positive results.


A detection method for lactic dehydrogenase activity in the inner ear.

A method for the detection of lactic dehydrogenase enzymatic activity in outer hair cells of the rabbit is described. The membranous labyrinth with temporal bone was prefixed in glutaraldehyde. After being placed into the incubation medium, it was postfixed in osmium tetroxide. Specimens of the organ of Corti were removed. Then the specimens were embedded in water-soluble glycol and cut with a cryostat for light microscopy, and also they were embedded in Epon and cut for light and electron microscopy. Sectioning of the membranous labyrinth was very easily made when the specimens were embedded in both the water-soluble glycol and the Epon. The structures of the frozen sections as well as the Epon-embedded ones were well preserved. In the frozen sections the preservation and localization of reaction products were thoroughly kept, but monoformazan of the Epon-embedded sections was soluble.


Gene activation of alcohol dehydrogenase in danio hybrids.

The regulation of alleles encoding the enzyme alcohol dehydrogenase (ADH) was investigated in F1Brachydanio hybrids (zebra danio female x spotted danio male) by acrylamide gel electrophoresis. Both parental species showed a single, cathodal band of species-specific ADH. During development at 26 degrees C, hybrid fry showed a preferential activation of the maternally derived Adh allele. It is suggested that the low activity of the paternally derived allele may result from an incompatibility between maternal regulatory factors and the paternal regulative element controlling gene expression.


A new dynamic model system for the study of capture reactions for diffusable compounds in cytochemistry. I. Description of the model with special attention to phosphate capture in acid phosphatase cytochemistry.

A model system is described for the investigation of the dynamics of precipitation processes in a matrix. In this system a solution containing the molecular species to be precipitated and the precipitating medium are pumped along opposite sides of a polyacrylamide film. The solutions flowing continuously along the film, interact and can form a precipitate inside the film. The applicability of the model was tested on the capture reaction for phosphate ions by the Gomori type medium for acid phosphatase. Precipitation of lead phosphate in the film occurred only at a phosphate concentration above a certain value. The dependence of this minimal phosphate concentration on various parameters was studied and the results were compared with values found in earlier model studies and calculations concerning phosphate concentrations that can be built up in lysosomes during the Gomori reaction. The system seems promising for obtaining fundamental data about other cytochemical enzyme trapping reactions as well as for the matrix facotrs involved in bone calcification and shell formation.


Characterization of rat bone marrow lymphoid cells. I. A study of the distribution parameters of sedimentation velocity, volume and electrophoretic mobility.

Various cell populations in rat bone marrow were characterized by means of a two dimensional separation using velocity sedimentation and free flow electrophoresis and by electrical sizing of the separated cells. Up to 4.5 mm/hr five different populations with discrete distributions in volume (coefficient of variation  10% to 13%) and sedimentation velocity (coefficient of variation 6% to 10%) were observed. Three of the small sized populations represented lymphocytes and small normoblasts and two of the larger sized populations represented myeloid cells. Almost all of these cells were in the G0/G1 cycle phase. In the faster sedimenting fractions which contained immature myeloid, erythroid and undefined blast cells and two S phase populations, discrete volume distributions were not evaluated. The cell populations with homogeneous volume (particularly the small lymphocytes) showed high density variations which condiserably impair the separation resolution. The cells sedimenting slower than 3.5 mm/hr were further separated by means of free flow electrophoresis into three peaks differing in electrophoretic mobility (EPM). The peaks of low and high EPM contained two populations and the peak of medium EPM contained three populations all characterized by normal volume distributions of uniform coefficient of variation between 11% and 14%. The small cells in the peaks of high and medium EPM were normolblasts and the other cells were lymphocytes. The biological significance of these results is discussed.


Characterization of the estrogen-induced uterine peroxidase by microelectrophoresis.

Estrogen-dependent peroxidase from rat uterine fluid has been investigated by microelectrophoretic techniques. The molecular weight of the enzyme has been determined in the range of 100,000 by using polyacrylamide gradient gels in the absence and presence of nonionic and anionic detergent. The isoelectric points are located between pH 4.5 and 5.9. Employing the two-dimensional combination of isoelectric focusing and gel gradient electrophoresis, the enzyme was separated into two subunits, one having a molecular weight of 70,000, the other less than 20,000. The large subunit has slight enzymatic activiy, while the smaller subunit may be responsible for the charge difference in the holoenzyme pattern. The glycoprotein pattern of the uterine fluid peroxidase is further defined by its separation by affinity chromatography using a concanavalin A-Sepharose column and by its susceptibility to neuraminidase treatment.


Retarded development of noenatal rat lung by maternal malnutrition.

Inadequate dietary intake during late pregnancy may have significant effects on the developing fetal lung which undergoes rapid cellular multiplication and differentiation shortly before birth. The morphology, glycogen distribution and acid phosphatase activity in normal and starved neonatal rats have been studied sequentially, by using histochemical and cytochemical methods. It has been shown that the normal pattern of lung growth and enzymatic development is retarded in neonates of malnourished mothers. A slowed rate of cellular division and differentiation in the critical prenatal period resulted in a more immature air-blood barrier at birth, with glycogen retention by some epithelial cells. Delayed Type 2 cell maturation with diminished acid phosphatase activity suggests a decrease in surfactant production in the malnourished newborn. In addition, fewer alveolar macrophages with reduced acid phosphatase activity were observed in the perinatal period of starved rats; this finding might have implications for the handling of inhaled bacteria shortly after birth. These results indicate that nutritional status of the mother has a marked effect on fetal lung growth and development by inhibiting cellular proliferation, differentiation and enzyme development by epithelial and macrophagic cells.


Peroxidase-positive endothelial cells in sinusoids of the mouse liver.

The cytochemical localization of endogenous peroxidase activity in sinus lining cells of mouse liver has been investigated. Kupffer cells, as identified by their exclusive ability to phagocytize large (0.8 micron) latex particles, exhibited strong peroxidase activity in nuclear envelope and endoplasmic reticulum. In addition, weak to moderate peroxidase activity was found in 57% of all endothelial cells. The enzyme in endothelial cells was also localized in nuclear envelope and endoplasmic reticulum, with a negative reaction in the Golgi apparatus. These observations indicate that peroxidase staining, as a marker for identification of Kupffer cells in mouse liver, is only of limited value and should be used in conjunction with other methods (e.g., latex phagocytosis).


A study of perceptions of rights and needs in interpersonal conflicts in homosexual relationships.

A pilot study was conducted to examine the issues of rights and needs in the interpersonal conflicts of couples of the same biological sexual orientation. The sample consisted of 91 men and 34 women who described conflict incidents that occurred in their relationships. The right most frequently perceived as an issue was participation in decision making. The need most frequently perceived as an issue was power. Issues were perceived more frequently as psychological needs rather than democratic rights. Almost no conflicts were resolved through negotiation.


Satisfaction in male homosexual couples.

Little information exists regarding factors in the satisfaction and stability of male homosexual relationships. On the basis of interviews with 14 male couples an open-ended questionnaire was developed concerning gay couples' relationships. This questionnaire and a modified version of the Marital Relationship Inventory, called here the Couples Relationship Inventory (CRI), were administered to 28 couples who described themselves as in a primary, relatively long-term relationship. Couples' descriptions of the successfulness of their relationships in conventional, heterosexual-oriented terms on the CRI were similar to their descriptions on the more homosexual-oriented, open-ended questionnaire. The study is seen as providing a point of departure for future research on gay relationships and the behavior transactions that may differentiate satisfying from unsatisfying relationships.


The identification of the social sex-role stereotypes.

The purpose of this study was to identify the social sex-role stereotypes that exist in the nation. The hypothesis for this study was that the stereotypes for femininity and masculinity are dimorphous. To determine the extent of dimorphism, four social sex-role stereotypes were examined: masculine men, feminine women, feminine men, and masculine women. Three hundred female and male respondents from all regions of the nation, who varied in age, ethnicity, education, sexual orientation, and social sex-role, completed a questionnaire by listing the characteristics people expected a masculine man, a feminine woman, a feminine man, and a masculine woman to have. The results supported the hypothesis that the stereotypes for femininity and masculinity are dimorphous.


Methodology for studying discrimination based on sexual orientation and social sex-role stereotypes.

This article presents the methodology used in collecting data for the study of the relationship of sexual orientation and social sex-role to the protection of civil liberties. The methodology is designed to determine how departures in sexual orientation and social sex-role are the basis for the abridgment of civil liberties. Departure in sexual orientation is defined as physical sexual activity involving individuals of the same sex. Departure in social sex-role stereotypes is defined as departing from or conforming to the feminine stereotype for men, the feminine stereotype for women, the masculine sterotype for men, and the masculine stereotype for women.


The relationship of assumption and knowledge of the homosexual orientation to the abridgment of civil liberties.

This article is concerned with the relationship of assumption or knowledge of departure from the heterosexual orientation to the abridgment of civil liberties. The methodology for obtaining data on sexual orientation is described. The definitions for assumption and knowledge of sexual orientation is described. The definitions for assumption and knowledge of sexual orientation are provided. The results indicated that: (a) the most frequently violated civil liberties were equality and procedural due process; (b) there was more assumption than knowledge of sexual orientation; (c) patterns of violations of civil liberties were similar whether sexual orientation was known or assumed; and (d) there was much more use of avoidance than any other mode of resolution, both when the sexual orientation was known and when it was assumed.


The relationship of departures in social sex-role to the abridgment of civil liberties.

This article examines the relationship of departures from the feminine stereotype for women and the masculine stereotype for men to the abridgment of civil liberties. The methodology is described for determining conformity to and departures from social sex-role stereotypes. Results show that: (a) there was more conformity than departure from social sex-role stereotypes; (b) there were proportionately more violations of the right of equality for those who departed; (c) sexual orientation was more often known for those who departed; (d) biological sex was not related to the violations of particular civil liberties for those who departed; and (e) for modes of conflict resolution, there was proportionately more use of avoidance by those who departed.


Attitudes toward homosexuality and femininity in men.

College students were asked to express their liking for a male stimulus person who was described as either heterosexual or homosexual and as masculine or feminine. Subjects disliked the homosexual man more than the heterosexual man, regardless of his gender attributes. Subjects did not, however, consistently dislike the feminine man more than the masculine man. The feminine heterosexual man was liked less than the masculine heterosexual man, but the feminine homosexual man was liked more than the masculine homosexual man. These results cast doubt on earlier notions that homosexual people are disliked because of their presumed inappropriate gender attributes. Instead, it appears that homosexual men are disliked even more if they violate the feminine stereotype. Reasons for this effect are proposed.


Lesbianism in female and coed correctional institutions.

Questionnaire responses from 13- to 17-year-old girls in four all-female and three coed institutions were used to determine rates and causes of institutional homosexuality. Rates were as high in coed as in single-sexed institutions. The overall rates of homosexuality for all seven institutions were 14% for "going with or being married" to another girl, 10% for passionately kissing, 10% for writing love letters, and 7% for having sex, beyond hugging and kissing, with another girl. The data suggest that previous homosexuality, often experienced in other correctional programs, explains much of the variance in institutional homosexuality.


Nonreproduction, homosexuality, transsexualism, and intelligence: I. A systematic literature search.

The relationship between various forms of nonreproduction (especially homosexuality) and intelligence (as measured by IQ and other tests) is investigated by a systematic review of the literature, selecting studies without regard to the direction of their findings. Most studies found the more homosexual subject groups' scores to be higher than those of the more heterosexual controls, and all exceptions to this trend are concentrated in one subgroup: prisoners. Moreover, the more representative the sample studied, and the less subject to challenge the methodology used, the clearer and more statistically significant was the superiority in intelligence of the more homosexual over the more heterosexual group.


Psychotropic agents in the management of chronic pain.

In recent years some psychotropic drugs have become useful aids in the management of chronic pain. In this paper these agents will be reviewed with respect to their mode of action, their indications and dosages. Problems in the assessment of their analgesic effects will be discussed.


Pain as abnormal illness behaviour.

In this article, the concept of abnormal illness behaviour is described, and chronic pain syndromes which represent variants of such behaviour are reviewed. Research using the Illness Behaviour Questionnaire has helped to elucidate aspects of chronic pain states, especially where a discrepancy exists between the patient's illness behaviour and the objectively assessed somatic pathology. Some comments are offered concerning the management of patients showing abnormal illness behaviour.


Hospital stress and patient reports of pain and physical status.

The Hospital Stress Rating Scale was used to measure stress due to the experience of hospitalization for 535 medical and surgical patients in a community hospital. Patients were also asked to rate the pain they experienced on a pain thermometer, and a recovery inventory was used to score patient self-reports of their physical status, both during hospitalization and subsequent to discharge. With statistical control for patient characteristics correlated with self-reports of pain and physical status, associations between hospital stress and these variables were observed. Patients scoring high in hospital stress tended to report more pain, lower physical status during hospitalization, and less improvement after discharge than patients scoring low in hospital stress.


Diagnosis of the patient with chronic pain.

The problems of classification of pain syndromes are briefly reviewed. A completely satisfactory taxonomy of pain has to be exhaustive and its categories must be mutually exclusive. This is not feasible in medicine in general and even less so in regard to pain which requires classification using several axes. Practical groupings of syndromes can be made which are helpful clinically. A list of these is provided, and principles which apply to the clinical investigation of patients with pain are stated.


Stress responses in male and female engineering students.

Male and female engineering students were studies under stress induced by a congnitive-conflict task and in a control condition spent in inactivity. The results showed that (a) in the control condition the sexes did not differ in adrenaline, noradrenaline or cortisol excretion, whereas heart rate was significantly higher in the females; (b) adrenaline excretion and heart rate increased significantly in both sexes during stress; (c) the rise in adrenaline excretion was more pronounced in the males, whereas the rise in heart rate was significantly greater in the females; (d) cortisol excretion increased significantly during stress in the male group only; and (e) self-estimates of effort and performance were consistently higher and increased more over time in the males than in the females, bu these sex differences on the subjective level were not reflected in actual performance. The interaction of biological and social factors in the development of sex differences in stress reactions is discussed.


A therapeutic milieu for chronic pain patients.

Traditional means of treating chronic pain have been unsuccessful in a discouraging number of cases. Pain centers have appeared within the last few years, offering a more comprehensive view of the whole pain problem. Pain centers address pain as a multifaceted event with social, economic, physiological, and psychological representations. In addition, the pain center constructs an atmosphere that provides every opportunity for reduction of pain, while minimizing those factors that tend to encourage its expression. Such a venture calls for a multidisciplinary approach; it further demands a rather sophisticated grasp of numerous factors which do not necessarily lend themselves to discussion with a single vocabulary. This paper will attempt to describe a number of conceptual models of chronic pain and to demonstrate how each of these models is addressed therapeutically in a multidisciplinary pain center millieu.


The binding of murine immunoglobulins to staphylococcal protein A.

The binding of murine IgG1, IgG2a, IgG2b, IgG3 protein molecules to Staphylococcal A protein is presented. Differential elution by sodium thiocyanate gradients is described. Proteins of the IgG2 class (either a or b subclass) require between 1.5 and 2.0 M for complete elution, whereas, IgG1 proteins are fully eluted with only 0.5 M NaSCN. These differential elution patterns can be utilized to distinguish between, or prepare proteins of different Ig classes. It is proposed that this quantitative rather than qualitative distinction between Ig subclass binding to Staph A, indicates the existence of multiple binding sites per molecule, with different markers of such sites being present on the heavy chain of the different Ig classes.


Normal mouse serum immunosuppressive activity: action on adherent cells.

The immunosuppressive activity of normal mouse serum (NMS) was evaluated by enumeration of primary in vitro plaque-forming cell response produced by normal spleen cell suspensions in response to sheep erythrocytes. The site of action of the inhibitor(s) was shown to be at the level of adherent cells since incubation of this cell type with NMS could reproducibly inhibit responses, whereas, in appropriate experiments, incubation of nonadherent cells with NMS had no effect. Furthermore, addition to normal spleen cells of excess spleen-adherent cells, normal peritoneal adherent cells, or of 2-mercaptoethanol could abrogate the inhibitory ability of NMS. Addition of excess nonadherent cells under the same conditions had no influence on inhibition. NMS was also shown to decrease the number of cell clusters, hemolytic clusters, and the number of nucleated cells recovered upon culture termination. It is suggested that NMS, acting on the adherent cell may hinder cell-to-cell contact and interactions.


Suppression of mitogen-induced proliferation of normal spleen cells by macrophages from chickens inoculated with Marek's disease virus.

Spleen cells from chickens 7 days after inoculation with Marek's disease virus (MDV) responded poorly to stimulation by phytohemagglutinin (PHA). Addition of these cells to syngeneic normal spleen cells caused of marked suppression of the PHA response of the normal cells. The MDV spleen cells also inhibited the DNA synthesis of MSB-1 lymphoblastoid cells in vitro. The suppressive activity is attributed to the presence in MDV spleen cells of a population of suppressor cells with characteristics typical of macrophages. The suppressor cell activity was not removable by treatment with anti-T or anti-B serum with C, but it was reversible by treatment with carrageenan or carbonyl iron/magnet, by passage through glass wool column, and by adherence to plastic Petri dishes. The adherent MDV spleen cells also showed strong suppressor cell activity against syngeneic normal spleen cells.


Tumor cell cytostasis by macrophages and antibody in vitro. II. Isolation and characterization of suppressed cells.

Tumor cells in a cytostatic state caused by macrophages and antibody were isolated. Such suppressed cells excluded vital dye, incorporated uridine and leucine, and metabolized glucose. They did not, however incorporate thymidine, nor did they resume cell division in culture. During prolonged culture, these cells eventually died. In this system, cytostasis was an all-or-nothing phenomenon at the level of the individual cell. Once in the cytostatic state tumor cells did not resume proliferation.


Trypan blue inhibits complement-mediated phagocytosis by human polymorphonuclear leukocytes.

Trypan blue completely inhibited attachment of human polymorphonuclear leukocytes (PMN) to Sepharose beads coated with C3 ant to sheep erythrocytes coated with IgM plus C3, but it did not inhibit attachment to erythrocytes coated with IgG. These results suggested that trypan blue inhibited C-mediated attachment to PMN membranes. Corroborative studies were performed with a strain of Staphylococcus aureus that requires C but not antibody, for opsonization and that activates the alternative pathway. Trypan blue was not toxic to PMN or bacteria, did nto interfere with immunoglobulin or C interactions, and did not affect attachment of opsonins to bacteria. However, the dye impaired PMN killing of S. aureus in normal nonimmune serum by inhibiting bacterial attachment to and ingestion by PMN. Further evidence that the inhibition was at the C3 receptor level came from the observations that, 1) once staphylococci were attached to PMN at either 37 degrees C or 0 degrees C, addition of trypan blue did not inhibit killing; and 2) trypan blue inhibited killing of bacteria opsonized with serum sufficient in C but previously absorbed at 0 degrees C with the same strain of organism to deplete specific antibody. Further studies with this agent may elucidate the roles of opsonic receptors on human phagocytes.


Specific suppression of anti-hapten reaginic antibody titers with hapten-coated liposomes.

Reaginic antibodies to DNP and ovalbumin (OA) were induced in B6D2F1 mice by a single i.p. injection of 1 microgram of DNP3-OA suspended with 1 mg of A1(OH)3 in 0.5 ml of saline. The anti-DNP reaginic antibody titers were markedly depressed by treatment of mice with DNP-coated liposomes. This treatment, however, did not affect the level of antibody formation to OA.


Role of interferon in the pathogenesis of viral diseases of mice as demonstrated by the use of anti-interferon serum. V. Protective role in mouse hepatitis virus type 3 infection of susceptible and resistant strains of mice.

Potent sheep anti-mouse interferon globulin has been used to determine the role of virus-induced interferon in mouse hepatitis virus type 3-infected susceptible (C57BL/6), semiresistant (C3H/He), and resistant (A/J) strains of mice. Injection of anti-interferon globulin accelerated the onset of death in C57BL/6 mice, induced almost 100% mortality in C3H/He mice that usually do not die of acute disease, and caused death in 4- and 6-week-old A/J mice, but not in older mice. We conclude that interferon is an important host defense factor in the initial response of different strains of mice to MHV-3 infection. Other factors, however, such as the capacity of macrophages to restrict viral multiplication probably underlie the genetically determined susceptibility or resistance of mice to MHV-3 infection.


Alteration of the fatty acid composition of membrane phospholipids in mouse lymphoid cells.

A simple method is described for introducing exogenous fatty acids into the membrane phospholipids of the murine leukemia cell EL-4, and into the membrane phospholipids of resting mouse lymphocytes. The method involves culturing of the cells with free or methylated fatty acids at concentrations up to 50 microgram/ml. The presence of serum in the culture medium does not interfere with fatty acid uptake, but does increase the growth rate and viability of the cells. Membrane lipid composition returns to normal after the cells are grown in medium without exogenous fatty acid. Fractionation of the cell membranes confirmed that exogenous fatty acids were incorporated into the phospholipids of the plasma membrane.


The requirement for the Fc portion of antibody in antigen-antibody complex-mediated suppression.

The role of the Fc portion of antibody in immune complex-induced suppression was studied in vivo and in vitro. BSA pheasant anti-BSA complexes, formed in antigen excess (Ag1Ab1), were found to suppress both responses to BSA and SRBC. When complexes were formed with F(ab')2 fragments of pheasant anti-BSA, no suppression was observed, indicating that the Fc piece was indeed essential for the induction of Ag-Ab complex-mediated suppression.


Studies on the terminal stages of immune hemolysis. III. Distinction between the insertion of C9 and the formation of a transmembrane channel.

The intermediate product EAC1-8 released cytoplasmic components as a result of at least two sequential reactions after its interaction with C9. Binding of C9 to EAC1-8 occurred in a few minutes even at 0 degrees C. Trypsinization of EAC1-9 prepared and held at low temperature resulted in nullification of the potential hemolysis of these cells. A brief incubation at 30 or 37 degrees resulted in the formation of an intermediate whose hemolytic potential could not be nullified by trypsin. The failure of trypsin to nullify hemolysis was attributed to the insertion of C9 into the cell membrane. Studies on the effec of EDTA or low temperature suggested that the reported temperature-dependent step in E* formation described by Frank et al. was the insertion of C9. The results of the studies with 86Rb-labeled EAC1-8 indicated that a transmembrane channel was not formed until after the C9 had been inserted and a further reaction or reactions had occurred.


Immunologic factors influencing the intra-tumor localization of ADCC effector cells.

The role of cellular and humoral immunity in the localization of blood-borne, bone marrow-derived ADCC effector cells into the T1699 mammary adenocarcinoma was investigated. Administration of ALG before tumor inoculation caused total immunosuppression and resulted in minimal in situ inflammation. ALG treatment started at the time of tumor inoculation suppressed the delayed hypersensitivity response below a detectable level but permitted a significant antibody response. Under these circumstances, the localization of ADCC effector cells into the tumors appeared normal. Similarly, administration of ALG at later stages of tumor growth, where ALG acts as an anti-inflammatory agent, did not interfere with the normal infiltration of ADCC effector cells in situ, although the delayed hypersensitivity response was totally suppressed. When melphalan treatment was used to produce tumor-bearing mice with an intact delayed hypersensitivity response but devoid of a significant antibody response, the drug-treated animals were found to have high levels of ADCC effector cells in situ. These results demonstrate that although the in situ inflammatory reaction appears to be immunologically inflammatory reaction appears to be immunologically dependent, neither the delayed hypersensitivity nor the antibody response is solely responsible for the localization of ADCC effector cells in the T1699 mammary tumor.


In situ lymphoid cells of mouse mammary tumors. I. Development and evaluation of a method for the separation of lymphoid cells from mouse mammary tumors.

A method of separating lymphoid cells from solid mouse mammary tumors was developed and evaluated. In this method the tumors are digested with 0.01% collagenase, 0.01% DNAase, and 0.025% trypsin in Dulbecco's PBS into suspensions of cells with a viability of 90%. The suspensions are fractionated on a continuous gradient of Ficoll in tissue culture medium. In model experiments this gradient was found to separate, cleanly, admixed cells of an established mammary tumor cell line and dissociated thymus glands. Recovery rates were 50% for the tumor cells and 80% for the thymocytes. The preparation of the cell suspensions and the gradient separation procedure are not harmful to the cells as indicated by trypan blue exclusion and the ability to grow in cell culture.


In situ lymphoid cells of mouse mammary tumors. II. The characterization of lymphoid cells separated from mouse mammary tumors.

Lymphoid cells were isolated from mouse mammary tumors by isokinetic gradient centrifugation. theta-bearing and ALS-sensitive cells were always the largest subpopulations found in these tumors. In fractions 4 to 6 from the isokinetic gradient, theta-positive cells ranged from 8 to 46% in autochthonous tumors and 14 to 47% in passaged tumors, and ALS-positive cells ranged from 34 to 46% in autochthonous tumors and 14 to 100% in passaged tumors. SIg-positive cells were only found in occasional tumors and, where present, averaged 2% of the separated population. Fc receptor-bearing cells averaged 15% and phagocytic cells averaged 8% of the cells found in fractions 4 to 6 of the gradient. Occasional ALS-positive and one-third of the Fc receptor-bearing and phagocytic cells also separated in fractions 7 to 10 of the isokinetic gradient. Although the individual tumors were very variable in the proportions of the various lymphoid subpopulations that they contained, this variability was a stable characteristic for at least one passage generation.


In situ lymphoid cells of mouse mammary tumors. III. In vitro stimulation of tumor cell survival by lymphoid cells separated from mammary tumors.

Lymphoid cells were isolated by isokinetic gradient centrifugation from mouse mammary tumors and evaluated for their ability to affect tumor cell survival with the microcytotoxicity assay. In all experiments stimulation of the growth or survival of tumor cells was seen. This stimulation was not seen with separated lymphoid cells treated with anti-lymphocyte serum and complement. The lymphoid cells separated from tumors were more stimulatory than were lymph node cells; even toxic to the tumor cells, the separated tumor-associated lymphoid cells were markedly stimulatory. The gradient procedure had no effect on the activity of sensitized lymph node cells, but it did separate cytotoxicity and stimulation into different fractions.


Macrophage-mediated in vitro sensitization of lymphocytes. II. The detection of neo-antigens on transformed lymphocytes and passages of normal fibroblasts.

Unprimed lymphocytes were sensitized in vitro by incubating them with syngeneic macrophages that had been fed with viral or cellular antigens. The sensitized lymphocytes were tested for their cytotoxic activity against virus-infected and noninfected fibroblasts. The antigenic preparations used for priming the macrophages were either tumor cell-free extracts or supernatants from virus productive cells. Cell-free extracts from the productive RadLV-induced lymphoma cells or the nonproductive radiation-induced lymphoma cells were immunogenic when presented to lymphocytes by macrophages. In contrast, cell-free extracts from normal thymocytes were much less immunogenic, suggesting that the presence of viral associated antigens (VAA) can selectively be detected on lymphoma cells by this assay. Fibroblastic cell lines but not primary fibroblasts were also susceptible to the cytotoxic lymphocytes induced by RadLV-fed macrophages. Primary fibroblasts became susceptible to the sensitized lymphocytes either after infection with the corresponding virus, or if not infected, after several passages in vitro, suggesting that neo-antigens cross-reacting with viral antigens appear during sub-culturing of fibroblasts in vitro. This system makes it possible to detect VAA either as immunogens when presented to lymphocytes by macrophages, or as targets for cytotoxic lymphocytes.


Structure and function of immunoglobulin domains. VII. Studies on the structural requirements of human immunoglobulin G for granulocyte binding.

The ability of various fragments of human myeloma IgG1 to inhibit rosette formation between human anti-D-coated human red blood cells and human polymorphonuclear leukocytes has been investigated. Although IgG and Fc showed a dose-dependent inhibition of rosettes and Fc showed a dose-dependent inhibition of rosettes at equimolar concentrations, neither of the fragments corresponding to the Cgamma2 and Cgamma3 homology regions obtained by acid-tryptic cleavage of Fc was able to inhibit rosette formation. The pepsin fragment of Fc, pFc', which represents the complete Cgamma3 domain, was also unable to prevent rosette formation. Reduction and alkylation of IgG or Fc markedly diminished cytophilic activity as measured by this system. These data indicate that the site in human IgG1 bound by granulocytes is dependent on the full quaternary structure of Fc, a requirement in marked contrast to that noted for binding by macrophages and monocytes.