The role of computerized tomography in the care of the injured.

The records of 377 patients with head injuries admitted to the South East Thames Regional Neurosurgical Unit in the two years following the introduction of the EMI scanner (computerized tomography) were examined. Each patient was assessed on the reason for transfer, the necessity for transfer, the safety of transfer and whether immediate access to a scanner would have altered management. The results show that the majority of patients were referred because of a  possible intracranial haematoma. Thirty per cent of the patients required operation, in 6 per cent transfer was unnecessary and 10 per cent arrived with inadequate resuscitation. It is suggested that with a wider deployment of head-dedicated scanners to some accident centres, the number of patients with head injuries at present being transferred to special units for assessment would be reduced substantially, with improvement of patient care.


Image intensifier as an aid to insertion of the Zickel nail apparatus for proximal femoral fractures.

Technical problems have been encountered when using the usual method of implanting the Zickel nail. We have attempted to overcome them by placing the patient on an orthopaedic table and screening the femur using an image intensifier. The results, and the particular ways in which this technique has helped, are described.


Axillary artery occlusion with erect dislocation of the shoulder.

Occlusion of the axillary artery occurred in a man with erect dislocation (luxatio erecta) of the shoulder. This combination of injuries has not previously been reported. The arterial injury was demonstrated by arteriography and repaired by a vein graft. There was an associated fracture of the greater tuberosity of the humerus, which required open reduction and internal fixation.


Acute nerve injury as a complication of closed fractures or dislocations of the elbow.

Twenty-one patients with acute injury of either the median, ulnar, radial, anterior interosseous or posterior interosseous nerves associated with 1540 closed fractures or dislocations of the elbow have been reviewed. The mean time of follow-up was three years and nine months. Nine patients, 6 of whom had ulnar nerve lesions, had persistent evidence of nerve damage. Five patients sustained median nerve lesions complicating supracondylar fractures of the humerus. One patient, in whom there was no evidence of nerve recovery at five months, made a full recovery following neurolysis. Of the 9 patients who sustained ulnar nerve lesions, 2 required neurolysis and anterior transposition of the nerve, at two months and five months respectively, to facilitate recovery. Except for one patient with persistent radial paraesthesiae, full spontaneous recovery occurred in 7 patients who had sustained either anterior interosseous, posterior interosseous or radial nerve lesions.


An improved orthopaedic stirrup.

A single RPH universal stirrup covers the many types of traction needed in orthopaedic practice. It reduces the torque load on the Steinmann pin, thereby diminishing the rate of loosening of the pin in the bone. From our experience with the stirrup at the Royal Perth Hospital, savings in ward costs can be achieved by shortening the time in hospital and reducing the need for antibiotic cover, by decreasing maintenance costs and by reducing the size of the appliance stock kept to cover the range of tractions used in orthopaedic practice.


Roadside medical care in Cambridgeshire.

The Mid-Anglia General Practitioner Accident Service (MAGPAS), established in 1972 to provide medical care at accident sites before the arrival of the ambulance and to assist ambulance crews with the severely injured, is discussed. Analysis of accident report forms returned by MAGPAS doctors is given.


Roadside care and rescue American style.

The development of an emergency services system in the USA is described, including the training of paramedical personnel. The increase in the survival rate of the severely injured appears to be impressive and it is suggested that lessons can be learned from the American system.


Development of an emergency services system--the Israeli example.

The Israeli emergency medical services system is discussed from the author's experience during a month's visit to Israel in July 1978. The impressive practical attitude of the Israelis to first aid has resulted from the threat of war and of terrorist activities, and has been influenced by developments in emergency care in the USA.


Individual and community: normative conflicts in the development of a new therapeutic community for older persons.

This paper examines the role of ideology in the establishment of a new therapeutic community for geriatric patients in a state mental hospital. Contradictory role expectations, reflected both in staff-staff and in staff-patient relationship, interfered with the program's achieving its stated goals. In order to apply a humanistic approach to programs for elderly patients, realistic goals-encompassing the range of patient competence-must be set within a reasonable time framework, and the program must provide for an appropriate range of structure.


Significant elements in the relationship of long-married couples.

A descriptive study of factors in the lifestyle of fifty couples married an average of 55.5 years and an average age of seventy-nine years provided data for this report. The non-random sample was heterogeneous using traditional socio-economic indicators. Life-Satisfaction (LSI-Z), Locke-Wallace Marital Adjustment scores, and perception of health were all high. More than half the sample were now or had been sexually active within the past five years. Independence, commitment, companionship and qualities of caring were significant elements in these long-lasting marriages.


Life-history interviews of aging gay men.

Homosexual men and women have seldom been studied by gerontologists and almost nothing is known about the lifestyles, pattern of development through the adult years, and the effect of homosexuality on aging. Fourteen gay men, ranging in age from fifty-five to eighty-one, were interviewed about their life history and experiences of aging as gay men. Three of the respondents had long-term relationships that lasted up to forty years; two had experienced the death of a lover and had begun a new long-term relationship; four had been married to women and two had children (one unmarried man adopted a son and is now a grandfather). The wide diversity of their patterns of aging, the presence of positive aspects of gay aging, and the high life satisfaction of many of the respondents contradict the stereotype of the lonely, isolated old gay man.


Ethnicity, aging and mental health.

The relationship between ethnicity and mental health deserves more consideration than it usually receives if we are to understand how values translate into behavior. This question could be explored productively with a variety of subgroups, but in this paper the emphasis will be on the aged. What is the relationship between ethnicity and the mental health problems of the elderly in American society? This paper offers some suggestions and reviews some data that might encourage further efforts in this area.


Young adults' relations with their grandparents: an exploratory study.

Some of the major aspects of the relationship between young adult grandchildren and their grandparents were examined. A sample of 269 undergraduate females, each with at least one living grandparent, responded to a survey instrument that assessed their current frequency of interaction and perceived emotional closeness with respect to each living grandparent. Results suggested that the grandparent's kin position relative to the grandchild was a critical variable, more so than the grandparent's sex, in influencing the intensity of the bond, Adult grandchildren tended to be significantly closer to maternal than paternal grandparents, and were significantly closer emotionally to the maternal grandmother than to each of the other grandparents. Also found was a wide variability among young adult grandchildren in their current involvement and emotional attachment to grandparents.


Change in significant other relationship and life satisfaction in the aged.

Changes in the choice of significant others from pre- to postinstitutionalization were examined in relation to life satisfaction in a population of well-aged retirement home residents. A total of fifty-nine residents, both male and female, ranging in age from 69 to 101 placed in three groups based on length of residence (Group 1 - up to 2 years, Group 2 - 3 to 8 years and Group 3 - 9 to 17 years), and asked to indicate whether a relative, or friend was most important to them at both pre- and postinstitutionalization. Reported preferences were examined in relation to subject scores on the Life Satisfaction Index - A (LSI-A). Significant changes in reported preferences, from pre- to postinstitutionalization were found only in Group 3, with the shift being away from relatives. LSI-A score differences between the groups were significant, only in the Group 1 vs 3 comparison. Analysis of LSI-A scores for residents who changed vs those who did not change pre- to postpreferences produced significant differences within the three groups with change individuals achieving higher scores in Groups 2 and 3. It was concluded that a shift in significant relationships away from the family may be an important factor in successful adjustment to a retirement home.


The relative effects of health and income on life satisfaction.

The relative effects of health and income on life satisfaction were analyzed using data collected in three recent national surveys. Financial situation was a slightly stronger predictor of life satisfaction for persons under age sixty-five, whereas health condition had a stronger impact on the life satisfaction of persons over age sixty-four. An attempt was made to quantify the relative impact of health and income on life satisfaction through an analysis of conjoint influence with contingency tables.


Methionine enkephalin and isosteric analogues. I. Synthesis on a phenolic resin support.

An efficient synthesis of methionine enkephalin using a phenolic resin support is described. Analogues modified at their C-termini, such as peptide acids, amides, methyl esters and compounds formed by their reduction, were prepared conveniently from common peptide phenyl ester resins. The resin was used in the synthesis of complex isosterically modified analogues designed to investigate the role of peptide backbone plays in receptor interaction. Free hexapeptide phenyl ester resins underwent intramolecular aminolysis liberating the corresponding cyclic peptides.


Structural biochemistry. 17. Synthesis of tryptophan dipeptides.

Boc-Trp-Leu hydrazide has been found to significantly inhibit growth of the L-1210 and P388 murine lymphocytic leukemias. A series of related tryptophan dipeptides were prepared and found to be devoid of such activity. The tryptophan dipeptides were most conveniently obtained employing the DCCI-NHS peptide bond-forming procedure.


Reduction of beta-endorphin content in the rat pituitary after dehydration and adrenalectomy.

Both dehydration and adrenalectomy of rats caused a significant reduction of immunoreactive beta-endorphin in the pituitary gland. Beta-Endorphin in hypothalamus, however, was not altered in either dehydrated or adrenalectomized rats.


Aminonitriles and aminothioamides related to natural amino acids.

N-p-Methoxybenzyloxycarbonyl and N-tert.-butyloxycarbonyl amino acid amides related to a series of natural amino acids were dehydrated to the corresponding Meoz- and Boc-alpha-aminonitriles. Deprotection of the latter derivatives afforded alpha-aminonitriles related to alanine, tyrosine, phenylalanine, dihydrophenylalanine, histidine, Dopa, ornithine, asparagine and glutamine. Thioamidation with H2S/NH3 or H2S/NEt3 in general converted the protected amino nitriles to Meoz- and Boc-alpha-aminothioamides. When deprotected these furnished the alpha-aminothioamides corresponding to alanine, tyrosine, phenylalanine, dihydrophenylalanine and histidine. For dehydration and thioamidation of histidine and Dopa, N alpha-Boc-im trityl-histidine and N-Boc-O, O'-diacetyldihydroxyphenylalanine were useful. Dopa was obtained as the free and Boc-thiohydrazide. Also prepared were N alpha,omega-diMeoz-ornithine DCHA, Meoz-2,5-dihydrophenylalanine DCHA and N,O-diMeoz-tyrosine as starting materials and N,O-dicarbobenzyloxycarbonyltyrosinamide, N,O-diZ-tyrosine nitrile and Z-beta-cyano-beta-alaninamide as model compounds. During deprotection of Meoz-alanine thioamide, transfer of an anisyl group from the N-Meoz protecting group to sulfur took place as a side reaction that yielded alanine p-methoxybenzyl beta-imidothiolic ester. This study provides two new series of amino acid analogs with potential antimetabolite activity. Also suitable for incorporation into peptide analogs, these afford approaches to relating structure and conformation to activity in biologically active peptides.


Synthesis and biological activities of [7-(azetidine-2-carboxylic acid)]-oxytocin and -lysine-vasopressin.

[7-(Azetidine-2-carboxylic acid)]-oxytocin and -lysine-vasopressin have been synthesised by a (6 + 3) strategy using protected hexapeptide acids with preformed disulphide bridges, and their biological activities have been investigated. All activities were reduced but not to the same extent. In assays of pressor and antidiuretic activity it was observed consistently that the responses to the vasopressin analogue were of shorter duration than responses to lysine-vasopressin of the same amplitude.


Synthesis of biological activity of human beta-endorphin analogs with disulfide bridges.

Three analogs of human beta-endorphin containing internal cystine bridges have been synthesized and their biological activities have been determined. It was found that the analogs with disulfide bridges between positions 17 and 26 or 11 and 26 retained full opiate activity as estimated by guinea ileum assay, whereas the analog with a cystine bridge between positions 7 and 26 showed reduced opiate activity. None of the analogs retained significant immunoactivity as revealed by radioimmunoassay.


Hydrogen bonds in crystal structures of amino acids, peptides and related molecules.

The results of a survey of 439 hydrogen bonds in 95 recently determined crystal structures of amino acids, peptides and related molecules suggest that the following generalizations hold true for linear (angle X-H---Y greater than 150 degrees) hydrogen bonds. (1) The charge on the acceptor group does not influence the length of a hydrogen bond. (2) For a given acceptor group, the hydrogen bond lengths increase in the order imidazolium N--H less than ammonium N-H less than guanidinium N-H; this order holds true for oxygen anion acceptor groups. Cl-ions and the uncharged oxygen of water molecules. (3) The uncharged imidazole N-H group forms shorter hydrogen than the amide N-H GROUP. (4) The carboxyl O-H groups form shorter hydrogen bonds than other hydroxyl groups. (5) The hydrogen bonds involving a halogen ion are longer than hydrogen bonds with other acceptors when corrected for their longer van der Walls radii. The observed differences between the lengths of hydrogen bonds formed by different donor and acceptor groups in amino acids and peptides, imply differences in the energetics of their formation.


Effect of sodium dodecyl sulphate on the 12S fraction of mustard proteins (B. juncea).

The effect of SDS on the 12S protein fraction of mustard seed (B. juncea) has been followed by the techniques of ultracentrifugation, gel filtration, gel electrophoresis, viscosity, ultraviolet difference spectra and fluorescence spectra. At low concentrations of SDS, up to 0.1%, both aggregation and dissociation of the protein occurs. Only dissociation occurs at higher SDS concentrations and is complete at 0.5% SDS. Viscosity increases sharply up to 0.15% SDS, remains constant between 0.15 and 0.30% and then increases markedly again. SDS induces also difference spectra with minima at 280, 288 and 295 nm. Fluorescence emission intensity increases at SDS concentrations less than 0.05% and quenching occurs at higher SDS concentrations. The results suggest that SDS causes association, dissociation and denaturation of the protein molecule.


Bovine neurophysin lipid complex. Their isolation, characterization and reaggregation.

A lipid-containing neurophysin fraction was isolated and purified from bovine posterior pituitary glands by acid extraction and affinity chromatography on a heparin-Sepharose 4B column. This lipid-rich fraction was found to be composed of noncovalent aggregates of neurophysin proteins and phospholipids such as phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine and sphingomyelin. The lipid-containing neuophysin was delipidated by treatment with choloform-methanol. The resultant apoproteins were characterized as bovine neuroions were developed for the reaggregation of purified bovine neurophysin-I and -II with lipids extracted from bovine posterior pituitary and hypothalamus and with synthetic lecithin. The resultant neurophysin lipid complexes have been shown to band upon isopycnic centrifugation at densities different from those of the respective purified bovine neurophysins.


Conformational analysis of the right-hand twisted antiparallel beta-structure.

The conformational analysis of a pair of two-linked peptide units in the anti-parallel arrangement is reported here with a view to study the effect of association of one chain with the other. The pair of two-linked peptide units were fixed in space through the hydrogen bonds between them, in accordance with certain hydrogen bond criteria. Model building was undertaken to ascertain whether the proximity of the side-chains could be used to eliminate any one of the right-hand twisted, left-hand twisted or regular beta-structures. Stereochemically, it was found possible with all of them. The preference for a right-hand twisted beta-structure, however, was indicated by the classical energy calculations. The relevance of the results thus obtained is discussed in the context of the preferential right-hand twist of the beta-pleated sheets present in globular proteins. The agreement between the minimum energy conformations obtained for the pair of two-linked peptide units and the globular protein data is also indicated.


Formation and synthesis of 3'-t-butyltyrosine.

During acidolysis by TFA of the t-butyl protecting group from Z-Tyr(But) or from Ser (But) in the presence of tyrosine, C-t-butylation occurred in the aromatic nucleus in Z-Tyr or tyrosine, respectively, to an extent of 0.5-1.0%. CF3COOBut formed during the acidolysis slowly C-t-butylates tyrosine. Tyr(3'But) is formed. The synthesis of Tyr (3'But) . HCl is described.


Synthesis of lysine-valinomycin by solid-phase segment condensation.

In order to obtain a readily derivatized analog of the ionophore antibiotic valinomycin, [1-lysine] valinomycin (Lys-VAL) was synthesized. The compound was built up on a polystyrene support by stepwise segment condensation and was cyclized in solution. The segments used were didepsipeptides protected by the t-butyloxycarbonyl and p-nitrobenzyloxycarbonyl groups. Derivatives prepared by acylation of the epsilon-amino group of Lys-VAL included [14C]acetyl-Lys-VAL, dansyl-Lys-VAL, palmitoyl-Lys-VAL and dithiodiglycoyl-bis-Lys-VAL. These derivatives had a high potassium binding capacity but were in general much less active than VAL in mediating ion transport in membranes.


Secondary implantation.

Aphakia is a disabling condition. It must be corrected by spectacles, contact lenses, refractive keratoplasty, or insertion of a pseudophakos. In those patients who do not tolerate a contact lens or cataract spectacles, refractive keratoplasty or secondary implantation of an IOL should be considered. Keratophakia and hyperopic keratomileusis have not yet evolved to the point at which refractive keratoplasty can be considered a practical answer to aphakia. This leaves a significant population of aphakic patients who can be visually rehabilitated only be secondary implantation of an IOL. Despite the risks and hazards associated with any intraocular surgery, the benefits derived from successful correction of aphakia with secondary implantation fully justify this procedure. Secondary implantation is a proved, successful modality for the corretion of aphakia. The number of patients requiring secondary implantation is not great, but in patients in whom secondary implantation is indicated, both the patient and the physician will find the results most rewarding.


The Ridley posterior chamber lens.

In summary, if used in the way I recommend, the Copeland lens gives excellent visual results and freedom from irritation. These results are sufficiently good that they need not be compared with the results of cataract surgery without implantation of a pseudophakos. It can be pointed out that these cases were all chosen with the idea of having a successful result. One cannot pick and choose all cataract patients in this way, but one can choose patients for lens implantation in this manner and thereby ensure excellent results.


Sensitivity to change in symptoms of new scales for rating chronic psychotic patients.

The sensitivity of Krawiecka, Goldberg and Vaughan's scales for rating chronic psychotic patients was established by testing them under conditions comparable to those of a controlled cross-over trial. 34 chronic schizophrenics were assessed on the scales at the beginning, after 6 weeks on one form of medication and after the same period on another drug. Significant changes in ratings of symptom severity of anxiety, hallucination delusions and incoherence were observed in patients who were sympomatic at the outset. Further, patterns of intercorrelations were found to be stable over time. It was concluded that Krawiecka et al.'s scales may be sensitive to change in symptoms in chronic psychotics and that given their known reliability, they may be viable research tools.


Factors influencing clinical response to psychotropic drugs. Imipramine in depression.

Numerous factors are involved in determining the efficacy of tricyclic antidepressant agents in the treatment of depression. The list includes diagnosis and patient selection; pharmacodynamics, bioavailability and tissue sensitivity; natural history; placebo response, nonspecific factors; compliance and adverse effects; the effects of concurrent life events, illness and treatment, and the bias in evaluating the outcome of treatment. Physicians should be aware of the factors that influence clinical response so they can maximize therapeutic effects and utilize the agents properly.


[Evaluation of AMP symptoms in independent and self assessment].

11 co-workers (9 physicians, 2 psychologists) of our research department with experience in the use of the AMP system participated in this study. According to the level of observation, 139 AMP symptoms were classified into 3 categories. 44 symptoms were classified into category SB (self-rating), 51 into category FB (psychiatrists' rating) and 44 into category SF (mixed rating). This showed the combination of different levels of observation in the AMP system. The symptoms of the 3 categories show an unequal distribution among the 9 AMP syndromes according to Baumann. The classification of the different symptoms according to the level of observation should be demonstrated during training sessions with the AMP system. This could avoid uncertainties in the judgement of AMP symptoms and improve the inter-rater reliability of the AMP system.


Studies on the use of blood lithium concentrations in lithium therapy in Japan.

The relationship between the concentration of lithium in whole blood and plasma was determined in 11 manic-depressive patients 12 h after the evening dose of lithium carbonate taken in the form of slow release tablets (Limas). Significant relationships were found between the concentration of lithium in whole blood and plasma as well as between the daily dose of lithium and the lithium concentration in whole blood and in plasma. The findings suggest that under certain conditions the concentration of lithium in a small sample of whole blood may be used to monitor lithium therapy in outpatients. The advantages of using whole blood are discussed.


Lithium effects on normal subjects. Relationships to plasma and RBC lithium levels.

15 normal subjects took lithium carbonate for 10 days in dosage sufficient to attain plasma lithium concentrations in the range of 0.7-1.4 mEg/l. Periodic blood specimens were analyzed for both plasma and RBC lithium. Subjects completed Profile of Mood States questionnaires every other day, and listed side effects. Just prior to beginning and ending the lithium trial, 10 subjects completed three tests of psychomotor function. Results indicate that such a course of lithium in normals induces dysphoric mood change and psychomotor slowing, without significant relationship to either plasma or RBC lithium concentrations.


Relationship between plasma, RBC, and CSF lithium concentrations in human subjects.

Simultaneous measurement of plasma, RBC, and plasma lithium concentrations took place with 17 inpatients chronically treated with lithium, at various times after the last lithium dose. RBC lithium levels were significantly higher than CSF lithium levels. Specimens drawn 10 or more hours after the last dose showed higher RBC and CSF lithium and lower plasma lithium than specimens drawn 4 or less hours after the last lithium dose. None of the lithium measurements differentiated manic-depressives from schizophrenics or schizoaffectives. Plasma, RBC, and CSF lithium all intercorrelated highly and equally.


[Lithium balance in mania].

Lithium balance studies were performed in 19 patients suffering from mania and 6 patients suffering from depression. The following results were obtained: (1) The mean daily requirement for lithium in the manic patients was 52 mM, in those with depression 30 mM (additional requirement in manic patients 73%). (2) Renal elimination of lithium, after optimal blood lithium levels had been reached, was 76% in mania and 97% in depression (retention in manic patients 21%). (3) In mania there was an unchanged lithium half-life time (12-13.5 h). (4) In mania and depression no significant differences in lithium and creatinine clearance were noted. (5) Standard diet or unrestricted sodium chloride administration did not significantly influence the lithium requirement or lithium retention. After exclusion of a renal or dietetic cause for increased lithium requirement or retention during mania, the existence of a 'lithium pool' dependent on the presence of a manic psychosis seems probable. As a result of this, somatic influences on endogenous psychosis have to be taken into account.


[Influence of diagnosis and length of hospitalization on the retest reliability for findings of the first psychopathological test (AMP system)].

80 inpatients were interviewed twice by the same physician with an interval of 24 h. By forming subgroups concerning diagnosis and duration of hospitalization, it was possible to examine the influence of these two criteria on the retest reliability of the following findings: (1) aspects of the interview; (2) AMP symptoms; (3) AMP syndromes; (4) clinical syndrome diagnoses, and (5) nosological diagnoses. The duration of hospitalization (up to 40 days) had no influence on the retest reliability of the described findings. The diagnosis (schizophrenia/depression) had no influence on the well-structured findings (AMP symptoms, AMP syndromes). On the contrary, the diagnosis influenced findings which are not so well structured and defined (aspects of the interview, clinical syndrome diagnoses, nosological diagnoses).


Antibiotic usage in Kupat Holim (Sick Fund) clinics. A pilot survey and evaluation.

A survey of antibiotic prescribing patterns of six physicians in two Sick Fund clinics was carried out during a one-month period. Over 1,200 antibiotic prescriptions and their therapeutic indications were reviewed. Ampicillin was the most commonly prescribed drug. Most of the prescriptions were for treatment of infections of the upper respiratory tract. Over one half of all antibiotic prescriptions were for presumably viral upper respiratory infections (URI), and half of all patients diagnosed as having viral URI received an antibiotic prescription. A method for measuring the quality of antibiotic usage by physicians in infections of the upper respiratory tract was developed and tested. The method is readily applicable to Sick Fund clinics and, in a trial run, proved capable of demonstrating large differences among physicians in their antibiotic prescribing habits for viral URI, tonsillitis, and otitis media.


Evaluation of hypertension control in general practice.

A review of the medical charts of 17 general practitioners in six family clinics in Tel Aviv showed that a blood pressure reading was recorded for 69.3% of the adult patients. Elevated values--greater than or equal to 160 (systolic) and/or greater than or equal to 95 (diastolic) mm Hg--were seen in 26.1% of the patients with recorded blood pressure readings. Antihypertensive medications were presecribed for 74.5% of those with elevated blood pressure. In two thirds of the treated group, blood pressure had been measured only once or twice before the initiation of therapy. Of the treated patients, 30% seem to have stopped therapy on their own initiative. Treatment was discontinued by the physician in 18%. Of the 52% who remained on treatment, only one third had a normal systolic or diastolic pressure on the last reading. The failure to reduce blood pressure in the other two thirds may be due, at least in part, to the use of methyldopa and reserpine without a diuretic.


Hematologic values in healthy older people in the Negev area.

Of 1,500 volunteers aged 65 or more selected at random in the Negev area, 224 were found to be healthy and available for further examination. The population represented broadly the various ethnic groups among Jews in Israel. The mean hemoglobin level, hematocrit, red and white blood cell counts and differential count showed no differences other than those due to chance when compared with the corresponding values in a younger (20 to 48 years of age) group. The differences between men and women were similar to those found in the comparison group, with men having higher mean values for hemoglobin, hematocrit, and red blood cell count; in the study group, serum iron, total iron-binding capacity and percent saturation were higher in men in all age groups. No differences were found in mean values of white blood cell count and differential count within the study population. However, mean serum iron was lower, and mean platelet count, higher than in the younger comparison group. This survey supports the use of normal standards for adults when elderly patients are examined, regardless of different ethnic origin. However, the higher than normal platelet counts in subjects from Asia, Africa and Eastern Europe should be taken into account.


Frequency of HLA-B8 in Israeli children with celiac disease.

The frequency of HLA-B8 in 18 Israeli children with celiac disease was examined and compared with that in 27 nonceliac children hospitalized at the time of the study. The diagnosis of celiac disease was made on the basis of an intestinal biopsy and a positive response to the lymphocyte inhibition factor (LIF) test, as well as a good response to withdrawal of gluten from the diet. HLA-B8 was found in 10 of the 18 celiac patients (56%). Of the 27 nonceliac children, three (11%) had HLA-B8. The LIF-positive celiac group had an HLA-B8 frequency five times greater than that in the nonceliac group, confirming our previous finding that the LIF test is a good diagnostic criterion for celiac disease. The combination of an intestinal biopsy, the LIF test and the presence of HLA-B8 could be a useful triad for diagnosis of celiac disease.


Ox kidney uricase: a new method of purification.

A purification procedure of uricase from ox kidneys has been worked out starting from a n-butanol extract. Gel filtration and ion-exchange chromatography give a highly purified enzyme, with a specific activity of 50, which corresponds to a 3,800 fold purification, and is almost homogeneous at the gel electrophoresis.


Ultrasonographic measurement of fetal growth parameters for estimation of gestational age and fetal weight.

The present study was undertaken to establish accurate, convenient, simple and rapid ultrasonographic measurements of fetal parameters and their correlation with gestational age and fetal weight. Relationship with gestational age was studied in 60 fetuses from 348 measurements of the biparietal diameter (BPD) and was also correlated with eight other parameters. There is no doubt that the BPD is the most reliable parameter for estimation of gestational age. In 34 infants, 306 measurements of nine fetal parameters taken within four days before delivery were correlated with their birth weights. Abdominal area gave the highest correlation, compared with other parameters or their combinations. Since combined measurements of several parameters gave only a slightly higher multiple correlation (R = 0.89) with fetal weight than the measurement of abdominal area alone (r = 0.85), their use is not justified as a routine procedure.


Rapid purification of human erythrocyte 6-phosphogluconate dehydrogenase by means of affinity chromatography.

A rapid two-step procedure is reported by which homogeneous 6-Phosphogluconate dehydrogenase can be isolated from human erythrocytes. This method is based upon direct affinity chromatography of the hemolysates on adenosine 2',5'-bisphosphate-agarose (yielding a 4,500-fold purification), followed by anion exchange chromatography on a micro-column of DEAE-Sephadex. The present method represents a considerable simplification over previously available procedures for the purification of this enzyme protein from human erythrocytes.


Effect of a 24-hour food-and-water fast on viscosity of whole blood and plasma.

As a result of abstaining from food and water for 24 h during the midsummer Tishah-b'Ab fast, blood viscosity increased by 16.5% in 27 subjects studied; plasma viscosity increased by 10.3% in nine; hematocrit, by 3.1% in 27; serum total protein, by 6.3% and albumin, by 7.7%, both in 29 subjects. These changes were statistically significant. Plasma fibrinogen did not increase significantly. Fasting-induced changes could impair blood supply to vital organs in patients with vascular insufficiency.


Thyroid function in hemodialysis.

The relevance of the biochemical evidence of hypothyroidism in patients on regular hemodialysis is debatable. Several indexes of thyroid function were assessed in 46 patients undergoing chronic hemodialysis for 2 to 45 months. Low serum levels of thyroxine were found in 69.5% of the patients studied and of triiodothyronine (T3) in 46.5%. Low values of T3-Sephadex binding were noted in 17.3%, of free thyroxine index (FTI) in 26.3% and of radioiodine uptake in 19.3% of the patients; 13.0% had high levels of thyroid-stimulating hormone (TSH). The overall incidence of hypothyroidism, based on FTI and TSH, was 34.8%. We concluded that the diagnosis of hypothyroidism should be based on FTI and TSH levels, and that chronically hemodialyzed patients tend to exhibit biochemical rather than clinical hypothyroidism.


Pica diagnosed by abdominal X-rays.

A young Arab woman with pica is presented. The diagnosis was suggested by the recurrent presence of numerous radiopaque particles in the course of the gastrointestinal tract. The particles changed location and concentration in successive abdominal X-rays taken at intervals of several weeks. Pica and its implications are discussed.


Heat and alkali induced hyperchromic effects in the ribosomal DNA of sea urchin (Lytechinus variegatus) sperm.

Melting point dispersion analysis of the ribosomal DNA (rDNA) of sea urchin (Lytechinus variegatus) sperm shows a scatter in the Tm obtained at wavelengths of 230-280 nm at 5 nm intervals. The dispersion curve of the rDNA, which lies in between human thymus DNA and T2 DNA, is probably suggestive of the intragenic heterogeneity. Alkali induced hyperchromic effects, examined over 220-290 nm at 5 nm intervals, show that the hyperchromic effect is minimum at 250 nm, followed by a sharp increase around 270-275 nm. These findings are consistent with the hyperchromic effects produced by alkali-melted G + C--rich DNA of lower organisms.


[Ataxia telangiectasia (Louis-Bar syndrome)].

Ataxia telangiectasia (Louis-Bar syndrome) represents a disease of childhood, characterized by diffuse telangiectasia of the skin and the conjunctivae and cerebellar ataxia. Patients are frequently predisposed for infections due to disturbances of the cellular and humoral immunity. This paper discusses the syndrome referring to an own observation of ataxia telangiectasia and literature of the past.


[Perioral dermatitis and the atopic constitution].

Detailed allergologic investigations, allergy histories and skin tests in 80 patients with perioral dermatitis led to the conclusion that the disease has to be considered a manifestation of atopy. Thus another clinical entity would have to be added to the hitherto known manifestations of the atopic constitution. The findings reported by other investigators may thus be interpreted as conditional or aggravating pathogenetic factors.


[Multiple basaliomas in pityriasis rubra pilaris].

This is a report of a 73 year old patient with pityriasis rubra pilaris and multiple basal cell carcinomas which especially appeared on the face. One of the tumors was located in the anal and perianal region. This is the first report of basal cell carcinomas in a patient with pityriasis rubra pilaris. Further observations will clarify whether there exists a closer relationship between the presence of basal cell carcinoma and pityriasis rubra pilaris. Therefore more attention should be paid to the existence of basal cell carcinoma in patients with pityriasis rubra pilaris.


[Kaposi's sarcoma and aplastic pancytopenia: simultaneous occurrence in a patient].

A 49 year old patient developed simultaneously a histologically verified Kaposi's sarcoma and an idiopathic aplastic pancytopenia. An association of these perhaps virus-induced diseases has not been reported until now. Consequences concerning the diagnostic approach of changes in peripheral blood cells in patients with Kaposi's sarcoma are delineated. Changes in peripheral blood and bone marrow in patients with Kaposi's sarcoma are discussed.


[Yellow-nail syndrome from d-penicillamine].

The yellow nail syndrom consists of the triad of scleronychia (yellow nails), lymphedema and respiratory tract involvement. The nail changes include symmetrical involvement of finger and toe nails with regression of the nail sheath, paling of the half-moon, arrest of longitudinal growth, thickening, yellow coloration, increased transverse convexity, ridging and partial separation of the nail. This report describes a case of drug-induced yellow nail syndrom due to penicillamin.


[Ulcus vulvae acutum with cold-agglutinin-positive, Mycoplasma-caused atypical pneumonia].

An observation of a febrile ulcus vulvae acutum clinically associated with an atypical pneumonia is described. As for the laboratory findings, the cold-agglutinine-titer was high (2:1,024) and the mycoplasma KRB was also positive (1:32).


[Elastofibroma cutis].

A 9 year old boy sought medical attention because of a cutaneous infiltrate of the nape that could not be diagnosed macroscopically. Microscopic examination showed it to be a fascicular fibroma of a special structure. The striking feature of the stroma was the distinct proliferation and degeneration of the elastic fibers. The alteraions of the elastic tissue are similar to those seen in elastofibroma dorsi. It is discussed if the lesion results from a hyperplasia of true elastic or/and pseudo-elastic fibers.


[Juvenile melanoma; a clinical and histological study].

Investigations were carried out in 52 female and 18 male patients, aged between 2 and 49 years, with juvenile melanoma. The tumor occured as a solitary tumor in all cases except one. It was mainly localized on the head (54%), and the extremities, on the trunk in three cases only. In 35% the tumor could be diagnosed clinically. Light microscopy revealed in 37.1% spindle cells, in 17.1% epitheloid cells, and in 45.7% the juvenile melanoma was of the mixed type. A proliferation of the vascular endothelial cells (37.1%) as well as immature looking mast cells in the tumor region were prominent findings. A transepidermal elimination of tumor cell nests was noted in one case. The other results are in agreement with earlier descriptions of this tumor.


[X-linked dominant chondrodysplasia punctata: an osteocutaneous syndrome].

A further case of X-linked dominant chondridysplasia punctata is described. This syndrome is characterized by the following cutaneous anomalies: congenital ichthyosiform erythroderma with thick, adherent hyperkeratoses; widespread atrophic skin lesions discernible after the first weeks of life; patchy alopecia; coarse and lusterless hair; onychoschisis. The hyperkeratoses of the newborn as well as the ensuing atrophoderma predominantly involve the hair follicles and are distributed in a bizarre linear or blotchy pattern. In some instances, a linear pattern of pigmentary disturbance has also been observed. These cutaneous signs and symptoms are so typical that the diagnosis of X-linked dominant chondrodysplasia punctata can be established even without X-ray examination. The syndrome has so far been observed exclusively in females. Apparently, the underlying X-linked gene defect is lethal in hemizygous males.


[Basaliomas after vaccination and infusion in basal cell nevus syndrome].

In a 31-year-old patient with a basal cell nevus syndrome two basal cell epitheliomas occurred through exogenous factors. The tumors appeared within 18 years after BCG vaccination and 3 years after an intravenous drip respectively. In basal cell nevus syndrome this course of development of basal cell carcinomas is seldom described.


[Folliculitis barbae candidomycetica from Candida parapsilosis].

In this case report the rarity of the causing agent, Candida parapsilosis, and its endothrix growth is accentuated. The unsuccessful application of local and systemic antimycotics required the surgical abrasion of the infected tissue.


[Tinea corporis from Trichophyton simii].

Tn unexplained, autochthone Trichophyton simii infection in Europe is reported. The patient was a 11-year-old caucasian girl, who had never been out of Germany, nor had contact with anyone from abroad. The girl's only pet, a guinea pig, was proven to be free of dermatophytes. Clinically the infection represented a somewhat persistent circumscribed lesion at the dorsum of one upper arm characterized mainly by small granulomata around hair follicles with only a slight erythematosquamous component.


[American cutaneous and mucous leishmaniasis (mucocutaneous leishmaniasis)].

The American skin and mucous membrane leishmaniasis or mucocutaneous leishmaniasis is induced through phlebotomus bites and occurs in South and Central America. Untreated severe multilating ulcerations of the face with involvement of oral, nasal, and pharyngeal mucous membranes may occur. Therapy consists of intramuscular administration of three- or pentavalent antimon preparations. In failure-patients amphotericin B is used instead.


A model of prehospital death from ventricular fibrillation following myocardial infarction.

Current efforts to reduce prehospital cardiac mortality focus more on deployment of specially equipped ambulances than on reduction of patient or ambulance delays. To evaluate this strategy, we needed to find a method that would isolate the separate effects of patient delay, ambulance delay, and the resuscitative capability of the ambulance. Using published data, we have generated a mathematical model of death from ventricular fibrillation following myocardial infarction that shows the relationship among these three factors. Analyses based on the model indicate that the potential life saving impact of a defibrillation-equipped ambulance is severely limited due to typical patient response patterns. If the ambulance arrives ten minutes after the onset of infarction, defibrillation capabilities will reduce prehospital mortality from 6 percent to 2 percent. After a more typical delay of 60 minutes, the mortality rises sharply to 13 percent for an unequipped ambulance. With a delay of this length, defibrillation capabilities reduce mortality only to about 12 percent.


Investment generation process in hospital facilities: the response of supply to capacity utilization measures.

Hospital investment behavior is commonly explained by means of either supply or demand factors. The inherent limitations of these models have led to ambiguous conclusions. This study applies a different approach, whereby investment generation is explained by means of a stock adjustment model. The model is empirically tested on a sample of New York City hospitals. Relative investment is found to be directly related to occupancy rate, indicating rationality in the hospital investment process. Scalar factors are also shown to be significant, implying the concept of preferred hospital size.


Silver-Russell syndrome. Observations in 20 patients.

The growth and development data of 20 patients with the Silver-Russell syndrome (14 boys, 6 girls) were analyzed. Family history, pregnancy and delivery did not reveal any significant anomalies. Birth length was 44.0 +/- 3.0 cm (boys) and 43.8 +/- 2.1 cm (girls), birth weight 2.0 +/- 0.4 kg and 2.05 +/- 0.3 kg, respectively. At the time of diagnosis (mean age 4.1 +/- 2.2 years), height was -4.4, bone age -1.9, weight -3.7, and head circumference -1.5 standard deviations below the normal mean for age. Calculated or reached adult height corresponded to 82--94% of target height. Intelligence was normal in most patients. 8 had asymmetrical extremities, 3 an asymmetrical face. 7 of 14 boys had cryptorchidism (3 uni-, 4 bilateral), 2 incomplete masculinization, and 2 of 6 girls hypertrophy of the clitoris. Development of secondary sex characters was appropriate for bone age with exception of one boy, whose puberty was early. In 3 boys with completed pubertal development, testicular volume was small and gonadotropins (before and after LHRH) high. It is concluded that 1. the growth pattern in Silver-Russell syndrome is quite homogeneous, and rather accurate predictions are possible; 2. Intersexual genitalia do not seem to be related to endocrine factors, and 3. hypergonadotropic hypogonadism appears to be frequent in males.


Cow's milk protein-sensitive enteropathy. Clinical and histological results of the cow's milk provocation test.

Nineteen infants suspected of having cow's milk protein-sensitive enteropathy were studied. They all showed failure to thrive, diarrhoea and/or vomiting when fed a diet of cow's milk, and improved when their diet was changed to casein hydrolysate. Jejunal biopsy was done before and 18--23 hours after a milk challenge. Of the 19 infants, 12 presented histological evidence of cow's milk protein intolerance. Eight suffered from vomiting and diarrhoea within 9 days of the milk challenge, but in 4 cases the histological abnormalities were not accompanied by clinical symptoms. In one case a chicken meat intolerance was documented. The histological appearance of the intestinal mucosa after chicken challenge was identical to that observed after milk challenge. In our opinion, repeated intestinal biopsies before and after an acute challenge is the best method to establish the diagnosis not only of cow's milk protein intolerance but also of intolerance to other alimentary proteins.


[Multifocal (chaotic) atrial tachycardia in infancy].

A newborn baby shows atrial tachycardia and gets into cardiac failure by atrial fibrillation at 12 weeks of age. With digoxin and chinidin spontaneous conversion to multifocal atrial tachycardia occurs. Treatment with additional propranolol leads to atrial fibrillation and paroxysmal atrial tachycardia with block. When chinidin was discontinued atrial flutter occurred. With a maintenance therapy with digoxin and chinidin the baby remained asymptomatic, and sinusrhythm occurred at 6 months of age. At 9 months chinidin was discontinued. At 14 months of age, the child is well and in sinusrhythm with a maintenance digoxin therapy. This seems to be the third described case of multifocal atrial tachycardia in infancy.


[Intrathoracic kidney in childhood with special reference to secondary renal transport in Bochdalek's hernia].

Three cases of thoracic renal ectopia are described. Remarkable is the case of an "acquired" congenital right diaphragmatic hernia (Bochdalek's herniation) with previously normally positioned kidneys. Classification is as follows: 1. real thoracic ectopia with normally developed closed diaphragm, 2. eventration of the diaphragm (relaxatio diaphragmatica), 3. diaphragmatic hernia: a) congenital diaphragmatic defects, b) acquired herniation (Bochdalek's). 4. traumatic rupture of the diaphragm with renal ectopia.


[Familial form of idiopathic hypercalciuria with nanism, bone and renal involvement in children].

A brother and a sister are reported, demonstrating the severe form of childhood idiopathic hypercalciuria associated with dwarfism, renal defects and bone lesions (rickets and osteoporosis). The family are Israeli Beduins from a small village with a highly inbred population. The parents who are first cousins, and the remaining 6 siblings are not affected. This suggests an autosomal recessive form of transmission for the trait.


Idiopathic arterial calcification of infancy.

A description is given of a 15-month-old girl with idiopathic arterial calcifications, detected during life time by X-ray. Radiological examination revealed calcifications in medium-sized arteries, histopathological examination showed distinct abnormalities of small vessels. The patient also had a metageria-like outward and retarded mental and motor development with myolysis. No cardiac failure was present.


[Clinical and biological evolution of myasthenia gravis after thymectomy in a 13-year-old girl].

A 13-year-old girl suffering from rhumatoid arthritis developed a myasthenia gravis. Her circulating anti-acetylcholine receptor antibodies were observed before and after thymectomy. Surgical removal of the thymus was followed by complete clinical remission associated with a progressive decrease of the antibody level; these antibodies did not disappear completely even as late as 2 years after the operation.


Viral hepatitis B and Wiskott-Aldrich syndrome.

A 4-year-old boy affected by Wiskott-Aldrich syndrome had overt viral hepatitis B after repetitive blood transfusions. He was given immune serum containing HBs antibodies, with only transient improvement. The HBsAg titer decreased immediately after each administration of immune serum, but 2 days later it was higher than before. This effect could be explained by the presence of HBs antigen-HBs antibody complexes in the immune serum, not detected by the current testing procedure.


Principle and method of kinetic microphotometric enzyme activity determination in situ.

An advanced apparative set-up is described for multipositional microphotometric recording of histochemical enzyme reactions in cryostat sections. It consists of a computer controlled microscope photometer with scanning stage. Measurements on the same tissue section may be performed at 12 preselected positions. These are repeatedly brought into the measuring beam in several measuring cycles. The complete measuring process, storage of measuring position coordinates, movements of the stage and statistical evaluation of the data is under computer control. By use of the gel film technique, extinction changes in tetrazolium coupled enzyme reactions can be measured continuously at initial rate conditions. Measurements are performed at identical conditions and can thus be analysed as relative enzyme activities.


Kinetic microphotometric activity determination in enzyme containing gels and model studies with tissue sections.

The dependence of microphotometrically recorded reaction rate on local enzyme concentration was studied as a basic prerequisite of comparative microphotometric enzyme activity determinations at initial rate conditions in tissue sections. Polyacrylamide gels containing defined concentrations of glucose-6-phosphate dehydrogenase served as a model. Optimal conditions of preparing enzyme containing gels are reported. Measurements in which either thickness of gel sections or enzyme concentration was varied proved the linear relationship between local enzyme concentration and microphotometrically recorded reaction rate. Sections of enzyme containing gels as well as cross-sections of rat muscles were used as models for studying possible influences of heterogeneous chromophore distribution (distributional error). No such influences could be detected during the initial phase of the staining reaction which suggests that distributional error is of no significance for kinetic microphotometric enzyme activity determination at initial rate conditions.


Histochemical demonstration of G6PDH- and SDH-activity on cultured fibroblastoid cells from rat pancreas using a "membrane technique".

The applied "membrane technique" represents a simple and time-saving method to provide evidence for presence of enzyme activities in single cultured cells. Substrates for G6PDH and SDH are filled on a semipermeable membrane stretched over a cylinder of plastic. The cells are cultured on a polyester foil and incubated for the enzyme staining reaction on the membrane. Enzyme products can be visualized under the microscope within the cells in definite areas.


Microphotometric studies on intraacinar enzyme distribution in rat liver.

Intraacinar distribution of succinate dehydrogenase (SDH), malate dehydrogenase (MDH), NADP-dependent isocitrate dehydrogenase (IDH), glutamate dehydrogenase (GluDH), lactate dehydrogenase (LDH) and NADH-tetrazolium dehydrogenase (TR) was studied in rat liver cryostat sections by multipositional microphotometric activity determinations. By statistical evaluation, activity of individual enzymes could be related to the acinar topography. Activity was evaluated with regard to distance of measuring position either from afferent (portal) or efferent (hepatic) vessels. Two independent distribution curves were obtained for each enzyme. Acinar distribution of all the enzymes studied followed sigmoid courses with maximal activity of SDH, MDH and LDH in zone 1 ("periportal") and GluDH, IDH, TR in zone 3 ("pericentral"). For all enzymes, maximum activity gradients were confined to zone 2 of the acinus. Data were also evaluated as ratios of activities in zone 1 and zone 3. The following ratios zone 1/zone 3 were obtained: SDH = 1.9, MDH = 1.7, IDH = 0.5, GluDH = 0.5, LDH = 1.3 and TR = 0.6.


The development of histochemically demonstrable cholinesterases in the rat neostriatum in vivo and in vitro.

The development of acetylcholinesterase (AChE) and non-specific cholinesterase (NsChE) activity was studied in the rat neostriatum by the light and electron microscope using three thiocholine methods. The AChE activity was first demonstrable only in the lateral parts of the nucleus, and during the early postnatal development the most intense activity was in the cell bodies, whilst the typical intense staining of the neuropil of adult animals was seen in two-week-old rats. Two types of AChE-containing cells were observed in the neostriatum of rats younger than two weeks and in cultures of newborn rat neostriatal cells. The neuropil of the cultures showed weak activity in the membranes of thin preterminal processes. In the neuropil of old rats, NsChE activity was present in the membranes of nerve cell processes. The capillary endothelial cells of newborn rats contained both AChE and NsChE. During subsequent development, the AChE activity disappeared, whilst for NsChE no change was seen in the distribution of activity seen in newborn or young adult rats less than three months old.


Cytochemical detection of catalase with 3,3'-diaminobenzidine. A quantitative reinvestigation of the optimal conditions.

The influence of various parameters of fixation and incubation upon the oxidation of DAB by catalase have been analyzed. Crystalline beef liver catalase was fixed with different concentrations of glutaraldehyde and peroxidatic activity was determined spectrophotometrically using DAB as hydrogen donor. Although aldehyde fixation appeared to be important in elicitation of the peroxidatic activity of catalase, the final pigment production after 60 min incubation was optimal with the lowest concentration of glutaraldehyde (1%), after the shortest fixation period (30 min), and at the lowest temperature (5 degrees C) tested. Similarly cytochemical studies with rat kidney sections incubated for 10 min confirmed that the staining of peroxisomes in proximal tubules was strongest after the "mildest" fixation conditions. The pH and the temperature of incubation were closely interrelated, so that at room temperature (25 degrees C) the maximal pigment production was obtained at pH 10.5, but incubation at 45 degrees C gave the strongest staining at pH 8.5. The production of pigment increased with higher DAB concentrations which required larger amounts of H2O2 in the incubation medium. Cytochemical studies on renal peroxisomes were in agreement with these biochemical findings. The observations indicate that there are several options for the localization of catalase depending on the fixation and incubation conditions. Hence, these conditions should be selected according to the tissue and the purpose of the study. Examples for such selective applications are presented.


REgional functional differentiation in the gut of the grasscarp, Ctenopharyngodon idella (Val.).

A regional differentiation--reflecting structural differences--of the intestine of larval and juvenile grasscarps can be illustrated by studying the activity of alkaline phosphatase and the uptake of orally administered horseradish peroxidase. Pinocytosis takes place in a welldefined area of about 23% of the length of the gut (segment II). Neither the rostral +/- 68% (segment I) nor the caudal +/- 9% (segment III) shows absorption of the enzyme. Alkaline phosphatase activity, mainly localized at the microvilli of the enterocytes is high in the first segment of the gut and low in the second segment. In larvae, the activity decreases sharply at the transition from segment I to segment II. The activity is weak or absent in the caudal third segment. Quantitative histochemical data are confirmed by biochemical analyses. Alkaline phosphatase activity is found all over the mucosal folds of the first segment, with relatively weak activity at the base and at the tip of the folds. This may be related to a renewal of the epithelium. Our results suggest that active absorption of digested food takes place mainly in the rostral first segment, while the uptake of macromolecules by pinocytosis is a function of the second segment. Comparison of the results with information available in literature leads to a rejection of the hypothesis that the uptake of protein macromolecules in Cyprinids is to be attributed to the absence of a stomach and therefore to an inefficient digestion of proteins.


Fine structural localization of thiamine pyrophosphatase and acid phosphatase activities in the mouse pancreatic acinar cell.

The fine structural localization of thiamine pyrophosphatase (TPPase) and acid phosphatase (AcPase) was examined in pancreatic acinar cells of fasting and fed mice. The results were not affected by these conditions. TPPase activity was positive in two and sometimes three cisternae of the inner Golgi lamellae as well as in the condensing vacuoles of the trans area, but negative in the rigid lamellae and small vesicles of the trans area. AcPase activity was demonstrated in two and sometimes three cisternae of inner Golgi lamellae, condensing vacuoles, rigid lamellae, lysosomes and smooth or coated vesicles in the trans area. The inner Golgi lamellae and the condensing vacuoles were positive for both enzyme activities. From these facts, the lysosome is considered to be formed not only in the GERL system but also through the rough endoplasmic reticulum-Golgi apparatus route. It is reasonable to consider that Novikoff's GERL is not independent from the Golgi apparatus but represents a part of this organelle.


The fluorescence and bright field microscopic demonstration of cathepsin B in human fibroblasts.

Cathepsin B was demonstrated cytochemically in human fibroblasts with Z-Ala-Arg-Arg-2-(4-methoxy)naphtylamide as substrate. The enzyme was visualized in the bright field microscope with the diazonium salt Fast Blue B as coupling reagent and in the fluorescence microscope with 5-nitrosalicylaldehyde. With both methods cathepsin B was found in small granules distributed throughout the cytoplasm.


The localisation of lead in the skin of light- and dark-adapted Xenopus laevis.

Toads pretreated for 2 months on either a dark or a light background were then exposed to lead nitrate at 50 ppm lead for 21 days, the illumination regimes being maintained. Metal analysis of dorsal skin showed significantly higher lead levels (p less than 0.01) in dark-adapted toads. No precipitated lead deposits were observed at the ultrastructural level, necessitating X-ray microanalysis of sections containing melanophores, gland cells and general (non-melanophore) cytoplasm. Analysis showed the lead to be concentrated within the melanosomes of the melanophores, and to be significantly higher (p less than 0.01) in individual melanosomes of dark-adapted toads than in light adapted ones. Copper was also found to be concentrated in the melanosomes and was higher (p less than 0.01) in the melanosomes of the dark-adapted toads. The results are consistent with the known affinity of melanin for heavy metals and the documented increase in melanophore number under prolonged dark background regimes. Since all toads received the same lead exposure, the melanosome results give rise to speculation that higher melanin levels might occur in individual melanosomes of dark-adapted skin.


Quantitative X-ray microanalysis of halogen elements in biological specimens.

Standards were prepared for quantitative X-ray microanalysis of the halogen elements Cl, Br and I in sections of resin embedded biological specimens. Halogenated aromatic compounds were dissolved in resin, which subsequently was polymerized. Homogeneity and stability of the standards were determined and found to be satisfactory. A general procedure of calculation of elemental concentrations according to the continuum method is given for the case that the specimen contains appreciable concentrations of one or more rather heavy elements. It is shown that use of approximations may lead to unacceptable errors, even in the concentration ranges occurring in biological specimens. As a practical application, the concentration of bromine in the chloroplasts of the red alga Chondrus crispus was determined quantitatively. The inner cells contained more bromine than the epidermal cells: in the chloroplasts of the inner cells bromine concentrations of about 6% could be demonstrated.


Mucosubstance histochemistry of Brunner's glands, pyloric glands and duodenal goblet cells in the ferret.

The mucosubstance of Brunner's glands, pyloric glands and duodenal goblet cells were studied using the various histochemical methods. The secretions of both Brunner's and pyloric glands were similar in their histochemical reactions. They contained neutral mucosubstances as in these glands in man. The duodenal goblet cells showed variations in their histochemical characters. (i) The secretions of most of the deep cells and the majority of superficial cells contained sialidase-labile and sialidase-resistant sialomucins. (ii) There were a few superficial and occasional deep cells, the secretions of which contained sulphated mucosubstances. (iii) There were some goblet cells, more in the villi than in the crypts, the secretions of which contained a mixture of sialomucins and a sulphated mucin. The sialomucin was mostly sialidase-labile and partly sialidase-resistant.


[Perennial rhinitis due to bacterial allergy (author's transl)].

Bacterial allergy causing perennial rhinitis remains a controversial nosological entity. Clinically, however, many cases of perennial rhinopathy do fit into the definition of this disease, and may represent a late stage of earlier inhalant allergy. It is characterized by positive delayed (type IV) skin tests with bacterial extracts as well as by a pronounced tendency to oedema and polyp formation. Topically applied steroids seem to provide sufficient prophylactic protection. Acute bacterial infections or any nasal surgery, however, require antibiotic treatment.


[The etiology of Menière's attacks and their consequent physiologic aspects].

On the basis of theoretical considerations and experimental studies, it can be shown that Menière's attacks result from rupture of the membranous labyrinth with diffusion of potassium into the perilymph and sodium into the endolymph.


The lung at high altitude.

Men and mammals (excluding the indigenous mountain species) who are born at high altitude, or who ascend to live there for a long period, have to undergo acclimatization which affects virtually every system in the body. Since chronic hypoxia is the most important adverse factor in the mountain environment, the lung plays a major part in the process and shows many alterations in structure and function. However, we remain ignorant about many aspects of acclimatization of the lung to hypoxia especially at the ultrastructural level with respect to those cells whose normal function is not yet established. An account of what is known is given in this paper.


[Middle and inner ear barotrauma caused by scubadiving (author's transl)].

On the basis of our own experiences and the current literature, the following guidelines were established for the evaluation of scubadivers: 1. The ENT physical examination must include otoscopy and the valsalva manoeuver. The scubadiver should be able to promptly and symmetrically inflate his middle ear spaces. A central perforation is a relative contraindication, while a marginal ear drum perforation is an absolute contraindication for scubadiving. 2. Recommendations to the diver: Ear pressure equalibration should be performed continuously with increasing and decreasing water depth. Ear plugs should never be used. 3. Management of diving injuries: Barotitis should be treated in a manner similar to acute otitis media. Transient vertigo while ascending (alternobaric vertigo) without nystagmus or hearing impairment needs no further vestibular examination. A middle ear exploration is indicated when there is suspicion of a perilymphatic fistula.


The distribution of I/i, Pr and Gd antigens in mammalian tissues.

The I/i Pr1-2 and Gd antigens are the targets of cold agglutinins. The expression of these antigens in the main organs of four mammalian species was studied by indirect immunofluorescence. Specific distribution patterns were observed and possible clinical aspects of the findings are discussed.


[Diagnosis of acoustic neurinoma (author's transl)].

In conjunction with a competent medical history, the early diagnosis of acoustic neurinomas is based on the following clinical investigations: pure tone and speech audiometry, caloric testing for nystagmus, and Stenvers radiological examination of the petrous bone. In abnormal studies, audiometry consistently indicates a unilateral hearing loss which is often accompanied by a severe speech discrimination impairment. The caloric response of the affected side is significantly reduced in 90% of the cases. In the same per cent of cases, the Stenvers projection shows significant pathological findings. These include enlargement of the internal auditory meatus, absence of the crista transversa and atrophy of the roof of the internal auditory meatus. As a result of these studies, the possibility of an acoustic neurinoma can be excluded in 50% of cases. Only when these tests yield enough positive findings should more sophisticated studies, such as computerized tomography and meatocysternography, be considered.


[Rhinolalia aperta after the use of a postnasal pack as a medico-legal problem (author's transl)].

A 23 year old patient needed a postnasal pack after tonsillectomy and adenoidectomy for control of severe postoperative bleeding. After removal of the pack on the sixth day, the patient had rhinolalia aperta. The patient could still swallow normally and there was no nasal regurgitation when swallowing solids or fluids. The patient sued for damage when 6 months of speech therapy failed to give any improvement of the nasal speech. The patient argued that the surgeon was negligent in leaving the postnasal pack in position for too long, and resulted in a paresis of the soft palate. However, a careful review of the record indicates that no real paresis of the musculature of the soft palate had occurred since the nasopharynx could be closed by the soft palate during deglutition. The patient's complaints were more likely psychologically induced, although there is no known case in the otolaryngologic literature on this syndrome in which phoniatric function did not recover spontaneously or after speech therapy.


[Esophageal complications following ventral cervical disc surgery (author's transl)].

A number of complications can occur following cervical vertebral fusions of intervertebral disc lesions. These include hypoglossal or recurrent laryngeal nerve paresis, inflammation of the operative wound, injuries of the esophagus and hypopharynx, as well as instability of alloplastic fusion material and spinal cord injury with tetraplegia. Cloward has reported transient postoperative dysphagia in 80% of patients. We describe a patient suffering from persistent dysphagia after cervical fusion. The symptoms were caused by adhesions between the esophagus and prevertebral muscles and a sharp edge of osteochondrosis of the vertebral body. The importance of surgical treatment in such patients is discussed.


Keratinocyte differentiation of human buccal mucosa in vitro.

The process of differentiation in keratinocytes of human buccal mucosa is accompanied by a number of specific membrane and cytoplasmic changes. Using simple tissue culture techniques, it has been possible to establish an in vitro model for keratinocyte differentiation which closely resembles the in vivo situation. A multi-layered structure is formed with maturation towards the surface. The superficial cells are characterized by a thickening of the plasma membrane, an increased concentration of tonofilaments, loss of intercellular adhesions and the presence of membrane-coated granules. It is concluded that this represents a suitable in vitro model for the biological and pathological study of both normal and abnormal oral epithelium.


[Endoprosthetic closure of pharyngeal fistulae (author's transl)].

The Häring endoprosthesis was originally designed for re-establishing an esophageal passage in patients with tumor stenosis of the esophagus. We have successfully used this prosthesis in two patients for closure of large pharyngeal fistulae following laryngectomy. The prosthesis provides a watertight closure of the pharynx and allows immediate natural feeding by the patient.


[Endonasal sinus surgery with endoscopic control for recurrent sinusitis in children (author's transl)].

Since 1976, 35 transnasal operations of the maxillary sinus with endoscopic control were performed in children as treatment for chronic sinusitis. After luxation of the inferior turbinate, a mucosal flap was developed to expose the lateral bony nasal wall. A sinus fenestra was then created to allow endoscopic control of the antrum during the operation. Gross pathological alterations of the mucosa were removed while most of the lining mucosa was preserved for recovery. 30 of the 35 children were followed from 4--18 months after surgery. 28 were without any discomfort. Although X-ray studies in many of the patients revealed mucosal swelling four weeks after surgery, the maxillary sinuses were well aerated 8 weeks after operation. Transnasal sinusotomy under endoscopic control is considered to be the treatment of choice for chronic sinusitis in adults and can now also be considered to be the treatment for similar disease in children.


[The pathogenesis of tracheal stenosis following thyroidectomy (author's transl)].

The frequency of trachealmalacia or stenoses following operations for struma or recurrent struma initiated our study of the pathogenesis of such changes. This study revealed that mechanical factors, such as compression of the trachea and the like, had been reported in the literature as the causative factors. The present paper investigates to what extent disturbances in the blood supply of the trachea, particularly after ligature of the inferior thyroid artery during thyroidectomy, influence changes in the tracheal mucosa, the connective tissue, and the adjacent cartilage. In twelve experiments on domestic pigs, the blood supply of the cervical trachea was interrupted. After varying periods of survival time, the animals were sacrificed and the tracheas histologically examined. In all cases, ischemic changes in the tracheal mucosa and cartilage could be found in addition to inflammatory reactions with scar formation. It seems justified to conclude that both mechanical factors and disturbances in local blood supply can cause tracheal tissue changes after thyroidectomy. The inferior thyroid artery and its branches also seem to play a central role in the success or failure of tracheal reconstructions following end-to-end anastomoses after stenosis resections. As a consequence, this paper also deals comprehensively with the detailed anatomy of this vessel.


[Postoperative mucocoeles of the maxillary sinuses (author's transl)].

The postoperative occurrences of mucocoeles of the maxillary sinuses are much rarer than those of the frontal or ethmoid sinuses. Their occurrence following Caldwell-Luc operations is 1:150. They may originate from obliteration of the sinus ostia (antritis dilatans) as well as from residuel mucosa in the upper lateral recesses of the sinus cavities. There are four clinical types of mucocoeles: (1) those which present with a swelling of the cheek, (2) those with destruction of the orbital floor with elevation of the eye, (3) those with destruction of the hard palate (which are extremely rare), and (4) those which present as multiple cysts (also rare). Among the author's 20 cases, there were 15 of the first group, 4 of the second and one of the fourth. In differential diagnosis, ameloblastoma of the maxilla must be considered. When suppuration (or pyocoele) occurs, the clinical picture may be similar to that of periorbital edema or inflammation. Operative treatment requires complete removal of the mucocoele and restoration of nasal drainage. When destruction of the orbital floor has occurred, reconstruction with a free septal cartilage graft is recommended.


[Cerebellar abscess as an intracranial complication of otogenic origin (author's transl)].

Three cases of otogenic cerebellar abscesses, treated between 1976 and 1978, are reviewed. Early diagnosis is the most important factor in the successful management of these lesions. In localising subtentorial intracranial abscesses, computed axial tomography appears superior to other neuroradiological methods. Lumbar puncture is both inaccurate in diagnosis and dangerous to the patient, when increased intracranial pressure exists.


[Malignant melanoma of the larynx (author's transl)].

Three instances of malignant melanoma of the larynx are reported. Tumour localization and infiltration are described and the differentiation from normal and pathological tissue changes is discussed. It is noted that distant metastases present late.


Diagnosis, relative incidence, and probable cause of cunean tendon bursitis-tarsitis of Standardbred horses.

A prospective study of the diagnostic results on 25 previously untreated, slightly lame Standardbred horses showed that manipulative tests are of some help in diagnosis. The lameness of each horse was diminished or the horse went lame on the opposite limb after being given an injection of anesthetic in the cunean bursa, and lameness improved more when local anesthetic was injected in the distal intertarsal and tarsometatarsal articulations. In four horses, lesions of the distal articulation of the hock were evident on radiography.


Treatment and prognosis for cunean tendon bursitis-tarsitis of Standardbred horses.

During 1975 and 1976, 70 Standardbred horses with cunean bursitis-tarsitis were treated by cunean tenectomy or by more conservative approaches (rested, given corticosteroid injections in the cunean bursa and distal hock articulations, given an oral course of phenylbutazone, a combination of these) or were sold. In 1975, owners were urged to have cunean tenectomy done to horses that appeared to have potential to race well, and in 1976, the more conservative methods of management and treatment were recommended. Statistical analysis of racing performance data led to the conclusion that there was no difference between the effect of these treatment approaches on racing performance during the rest of the year and the following calendar year.


Mesodiverticular bands as a cause of small intestinal strangulation and volvulus in the horse.

Volvulus of the jejunum and ileum in three horses was associated with intestinal strangulation in a mesenteric rent. The rent was in the jejunal mesentery at its point of attachment with an anomaly that was classified as a mesodiverticular band. The band also was attached to the dorsolateral surface of the jejunum, thus forming one side of a triangular hernial sac that was completed on the other side by the adjacent jejunal mesentery. Incarceration of a loop of small intestine in the hernial sac preceded rupture of the jejunal mesentery and subsequent intestinal strangulation. Surgical correction was successful in two horses and involved resection of the gangrenous intestine, then jejunocecal anastomosis. The third horse was euthanatized when intestinal rupture and peritonitis were found on exploratory laparotomy. Two mesodiverticular bands attached to the distal jejunum were incidental necropsy findings in a fourth horse.


Acute oral marijuana poisoning in the dog.

Ingestion of marijuana by three dogs in unrelated incidents resulted in depression-type toxicosis in each case. The most evident clinical signs were central nervous system depression and ataxia. Emesis and hypothermia were noted in two of the cases. Symptomatic and supportive treatment was accompanied by clinical improvement. In two cases, recovery was slow, with clinical signs apparent for 36 to 48 hours after onset. In the third case, clinical signs were apparent for only 3 hours.


X-ray-intensifying screen technology for improving veterinary field radiography.

Ultra-high-speed rare earth x-ray-intensifying screens were used to increase the efficiency of field radiography in an equine research project. The usefulness of these screens to the large animal practitioner was evaluated.


Ehrlichiosis in wolves, dogs, and wolf-dog crosses.

Canine ehrlichiosis was diagnosed in wolves, dogs, and wolf-dog crosses at a small zoo in northcentral Florida. Five of 9 adult canids and all 8 pups confined to a common kennel died as a result of the infection. The epizootic was associated with a massive Rhipicephalus sanguineus infestation. Hematologic and pathologic findings in an adult wolf-dog cross that died were characteristic of canine ehrlichiosis. That animal and four other canids were seropositive for Ehrlichia canis. No deaths occurred following the oral treatment of remaining canids with tetracycline hydrochloride and the treatment of canids and premises with acaricides.


Serologic survey for leptospirosis in coyotes in northcentral Kansas.

During 1975 to 1977, serum samples were collected from 101 adult coyotes (Canis latrans) captured in northcentral Kansas. Ten samples were seropositive by microagglutination testing and six of those samples were seropositive for multiple serovars. Titers for Leptospira interrogans serovars grippotyphosa, pyrogenes, djasiman, butembo, and pomona were demonstrated.


Dirofilaria immitis infection in foxes and coyotes in Indiana.

Between October 1976 and January 1977, carcasses or hearts and lungs from 202 wild foxes and coyotes in Indiana were collected to determine the prevalence of Dirofilaria immitis infection. Three of 113 (2.7%) red foxes (Vulpes fulva), 3 of 81 (3.7%) gray foxes (Urocyon cinereoargenteus), and 1 of 8 (12.5%) coyotes (Canis latrans) were infected with D immitis. An average of 5 worms (range, 1 to 10) were recovered from foxes and 11 worms were recovered from the coyote. Worms from the coyote were of normal length and all female worms contained developing embryos and microfilariae. Worms from foxes were small, and none of the female worms contained developing embryos.


Serologic profile of exotic deer at Point Reyes National Seashore.

Serotests were conducted on axis (Axis axis) and fallow (Dama dama) deer at Point Reyes National Seashore to determine their status with respect to nine diseases enzootic to the native black-tailed deer (Odocoileus hemionus columbianus) or to resident dairy cattle. In the exotic deer, the proportion of animals that were seropositive included: anaplasmosis, 35%; bluetongue, 48%; brucellosis, 0%; bovine viral diarrhea, 2%; infectious bovine rhinotracheitis, 3%; leptospirosis, 7%; parainfluenza-3, 49%; toxoplasmosis, 8%; and Q fever, 51%. The prevalence of antibodies among a small sample of the black-tailed deer included anaplasmosis, 100%; toxoplasmosis, 29%; and Q fever, 57%. The antibody prevalences in a sample of dairy cattle in the area included anaplasmosis, 19%; toxoplasmosis, 8%; and Q fever, 100%.


Observations on diagnostic tests for paratuberculosis in a deer herd.

Paratuberculosis was diagnosed in a herd of 62 fallow deer (Dama dama) and 38 sika deer (Cervus nipon) maintained in an animal park in Ohio. A comparison was made of delayed-type hypersensitivity responses, results of in vitro lymphocyte immunostimulation tests, and isolation of Mycobacterium paratuberculosis on fecal culture in 19 deer.


Hyaluronidase as an adjunct in an immobilizing mixture for moose.

Hyaluronidase was put into immobilizing syringes for 58 of 104 moose captured with a fentanyl/xylazine mixture. Induction times were measured for both groups and were related to injection site as well as drug mixture. Hyaluronidase-treated moose had significantly shorter induction times than others. Injection site also had a significant effect on induction times.


Acute viral hepatitis in California sea lions.

Acute viral hepatitis was diagnosed in five California sea lions (Zalophus californianus) stranded along the Los Angeles coast. Light microscopy revealed large nuclear inclusion bodies in hepatocytes. Electron microscopy provided evidence that these inclusion bodies were composed of adenovirus-like virions. Attempts to grow the virus in cell culture systems were unsuccessful.


Hepatic trematodiasis in a Ganges River dolphin.

Hepatic trematodiasis caused by Cyclorchis campula was diagnosed in a juvenile Ganges River dolphin that had been in captivity at an aquarium for approximately 1 year. Histopathologic findings were severe chronic suppurative cholangitis, hyperplasia of the bile duct epithelium, and periductal fibrosis associated with fluke infection of the large bile ducts.


Mycotic pneumonia in mariculture-reared green sea turtles.

Microbiologic examination of 29 juvenile green sea turtles with a buoyancy abnormality revealed pulmonary infection with Sporotrichium sp, Cladosporium sp, and Paecilomyces sp. Histologic examination of the lungs revealed granulomatous lesions containing branching septate hyphae. The diagnosis was mycotic pneumonia.


Fatal Beauveria bassiana infection in a captive American alligator.

The entomopathogenic fungus, Beauveria bassiana, was isolated from pulmonary lesions of a dead American alligator (Alligator mississipiensis) at the Oklahoma City Zoo. Colonies of the fungus, which had sporulated in vivo, were found in the thoracic air spaces. Septate, branching hyphae and fungal spores were seen in stained histologic sections of pleura and lung. Dissemination to other viscera had not occurred. This case indicated that B bassiana, a rare vertebrate pathogen, may be a fatal mycotic agent in captive reptiles.


Avian malaria in African black-footed penguins.

Ten captive-reared African black-footed penguins (Spheniscus demersus) from a large outdoor colony were monitored for avian malaria, using several diagnostic tests. One treatment regimen was evaluated. Thin smear blood evaluation enabled detection of seven parasitemias involving Plasmodium relictum and Plasmodium elongatum in the penguins. Leukocytosis (relative lymphocytosis) was characteristic of infected birds. Parasitemia was detected as early as 21 days prior to onset of clinical signs (depression, anorexia, regurgitation, pale mucous membranes, and respiratory distress). The single bird that died had clinical signs only a few hours prior to its death. Treatment consisted of 0.03 mg of primaquine phosphate base/kg body weight, administered orally once daily for 3 days. Oral chloroquine phosphate therapy, given simultaneously, was administered in an initial loading dose of 10 mg of chloroquine phosphate base/kg body weight, followed by doses of 5 mg/kg at 6, 18 and 24 hours after the initial chloroquine dose. This treatment regimen prevented mortality and cleared parasites from the blood. Recurrences of malaria occurred in two birds that had received this treatment.


Disseminated granulomas caused by an unidentified protozoan in sandhill cranes.

Oral granulomas were observed in 31 (33%) of 95 captive sandhill cranes (Grus canadensis) at the Patuxent Wildlife Research Center. Necropsy of six of the afflicted cranes revealed granulomatous nodules throughout many of their organ systems. Intracellular protozoan organisms morphologically resembling schizogonic stages were observed within the granulomas by light and electron microscopy. Sexual and asexual stages of coccidia were seen in sections of the intestines of 4 of 5 cranes examined microscopically, and Eimerian oocysts were seen in fecal flotation specimens from 3 of 4 birds.


Ketamine/xylazine anesthesia in the pregnant rat.

Pregnant albino rats were anesthetized with a combination of ketamine and xylazine. A minimal effective dosage of 90 mg/kg for ketamine and 10 mg/kg for xylazine provided satisfactory anesthesia in 92% of the subjects, without adverse effects on gestation or the fetuses.


Necessity for end colostomy with transection of the descending colon in guinea pigs.

Surgical procedures for resection and transection of the descending colon in the guinea pig were studied. Wedge resection with removal of less than 1 cm diameter of tissue resulted in an 80% (8 of 10 guinea pigs) survival rate. Colonic transection with restorative anastomosis, either end-to-end or side-to-side, resulted in a 94% (35 of 37 guinea pigs) death rate due to colonic impaction proximal to the site of anastomosis. A modified procedure for ventral end colostomy resulted in a 79% (19 of 24 guinea pigs) survival rate. These results indicated that caution should be taken in surgical manipulations intended to treat or study diseases of the colon in the guinea pig.


Gingival hyperplasia induced by diphenylhydantoin in a gorilla.

An adult male lowland gorilla had been treated with diphenylhydantoin for 6 months following several acute convulsive episodes. The gorilla remained clinically normal during that period. Then, for no apparent reason, it refused its usual diet. Physical examination revealed acute inflammatory gingival hyperplasia. Full mouth gingivectomy and antibiotic and analgesic therapy resolved the oral inflammation and the anorexia.


Serologic survey for Toxoplasma gondii in wild animals in Florida.

Blood samples were collected for serum separation from 114 species of wild animals (25 species of mammals, 82 species of birds, and 7 species of reptiles) in Florida. Each of the 3,471 samples was tested for antibodies to Toxoplasma gondii, using the indirect hemagglutination test. The highest prevalences of T gondii antibodies were 19% in armadillos (Dasypus novemcinctus), 18% in raccoons (Procyon lotor), 13% in black rats (Rattus rattus), and 11% in opossums (Didelphis marsupialis). Antibody prevalences were significantly higher in male than in female raccoons (P less than 0.05) and in adult than in nonadult raccoons and opossums (P less than 0.005). A high proportion of seropositive animals was found in three other mammalian species: 4 of 4 black bears (Ursus americanus), 2 of 3 bobcats (Lynx rufus), and 2 of 8 Norway rats (Rattus norvegicus) tested. Antibodies were found in 8 of the 1,279 avian serums; they were not found in any of the 13 reptilian serums tested. There were no significant geographic variations in antibody prevalence in any species.


Bone conduction mechanisms: Mössbauer measurements on the role of ossicular inertia.

The Mössbauer technique was used to measure displacements of the stapes footplate and adjacent temporal bone during bone conduction stimulation at frequencies from 250 to 400 Hz in anaesthetized guinea pigs. The stapes was found not to be driven at amplitudes or phases that differed significantly from those of the temporal bone. Measurement of stapes displacements during air conduction stimulation, and of temporal bone displacements during bone conduction stimulation producing matching cochlear microphonic amplitude, enabled calculation of limiting values of amplitude and phase difference necessary to produce the required relative displacement. The obtained values (less than 1 dB for amplitude and 1--4 degrees for phase) were beyond the resolution of the measurement system employed for reasonable nuclear counting times. The results provide quantitative estimates of the magnitude of inertial effects, but do not establish whether ossicular inertia is an important factor in bone conduction stimulation.


Funneling mechanism in hearing.

The funneling mechanism in hearing is reviewed and its underlying mechanism clearly elucidated. It is concluded that GABA is a principal candidate among the putative transmitters.


A model of loudness summation applied to noise-induced hearing loss.

The main contention of this paper is that Zwicker's model of loudness summation is applicable to observers with noise-induced hearing loss when certain parameters of the model are modified. Two types of measurement were obtained in observers with normal hearing and noise-induced hearing loss: loudness summation as a function of level and narrow-band masking. These measurements provided a basis for modifying the parameters of the model. Results suggest that the model of loudness summation is applicable to observers with noise-induced hearing loss when the presence of recruitment and reduced frequency selectivity is taken into account.


Action potential suppresion, tuning curves and thresholds: comparison with single fiber data.

The collection of compound action potential (AP) threshold curves and their use to define the sensitivity of individual animals are described. Forward masking AP tuning curves (APTC) have also been collected in the chinchilla. Characteristics of APTCs are compared with single fiber frequency-threshold curves (FTC) in the same group of animals. The two sets of data are quite similar when the probe frequency used to collect the APTC is equated with a fiber's characteristic frequency (CF). The major difference is that APTCs are usually broader than FTCs. A paradigm utilizing two maskers in a forward masking situation, developed to study psychophysical unmasking [19,37], has been modified for measuring AP suppression. AP suppression areas are described as similar to single fiber two-tone suppression areas when probe frequency and CF are above 3 kHz. Relationships among single fiber, AP and psychophysical thresholds, tuning curves and suppression areas are discussed.


Calculating virtual pitch.

A procedure for the schematic and automatic extraction of 'fundamental pitch' from complex tonal signals, such as voiced speech and music, has been developed. While the auditively relevant 'fundamental' of a complex signal cannot be defined in purely mathematical terms, an existent model of virtual-pitch perception turns out to provide a suitable basis. The procedure comprises the formation of determinant spectral pitches (or 'fundamental frequency') from those spectral pitches. The latter deduction is accomplished by a principle of subharmonic matching, for whose realization a simple, universal and efficient algorithm was found. While the calculation may be confined to the determination of 'nominal' virtual pitch, certain typical auditory phenomena, such as the influence of SPL, partial masking and interval stretch, may be accounted for as well, in which case 'true' virtual pitch is obtained. The procedure operates on the frequencies and amplitudes of the signal's spectral components, is suitable for implementation on readily available programmable calculators and other arithmetic computers, and may be used in real-time 'fundamental-pitch' extraction as well. The procedure's performance and its applicability to the research and engineering of auditory communication are illustrated by some examples.


Intracellular recording of 'chopper responses' in the cochler nucleus of the cat.

Intracellular recording of 'chopper responses' in the cochlear nucleus of the anesthetized cat presented a sustained depolarization accompanied by spikes that lasted as long as the stimulation.


Semautomated method for the analysis of chlorpheniramine maleate tablets: collaborative study.

A semautomated method for the analysis of chlorpheniramine maleate tablets, which is based on the USP XIX general assay for salts of organic nitrogenous bases, was collaboratively studied by 6 laboratories. Collaborators were supplied with 4 composites from 4 manufacturers. In the method, the active ingredient is dissolved in dilute HCl, sampled, made basic with dilute NaOH, and extracted with isooctane. The isooctane phase is resampled and the drug is re-extracted into dilute HCl. The absorbance of the acidic aqueous solution is measured at 265 nm. The Associate Referee assayed the collaborative samples to compare the semiautomated and USP XIX methods, and found close agreement in the results from the 2 methods. In the collaborative study of the semiautomated method, there was excellent agreement of the results obtained by the collaborators. The coefficients of variation ranged from 0.46 to 2.24%. The method has been adopted as official first action.


Infrared estimation of fat, protein, and lactose in milk: evaluation of multispec instrument.

Thirty-eight milks (including homogenized and unhomogenized herd milks, individual cow milks, and packaged milks) were pre-analyzed by accepted standard methods and then analyzed in duplicate with a Multispec instrument on 2 different days. For the combined data, mean differences between duplicates were 0.01% or less within days and 0.025% or less between days. Standard deviations of difference between duplicates were 0.02% or less. Overall mean differences between Multispec and standard results were 0.02% or less with standard deviations of difference of 0.041%, but means for protein in packaged milks were 0.038% lower and means for lactose in individual cow milks were 0.04% higher than standards. Standard deviations of difference for fat were lower (0.016%) than average for packaged milks and higher (0.06%) than average for individual cow milks. Differences between Multispec results for homogenized and unhomogenized samples of the same herd milks were small, averaging 0.013% for fat, 0.005% for protein, and 0.011% for lactose. Regression equations for the combined data gave standard errors of estimate of about the same magnitude as the standard deviations of difference. Single calibrations would give good estimates for all of the types of milk analyzed, but for best accuracy the instrument should be calibrated with the type of milk to be analyzed.


Performance specifications for infrared milk analysis.

Maximum limits of 0.02% are recommended for precision of IR analysis for fat, protein, and lactose, and 0.04% for total solids. Recommended maximum systematic errors are 0.06% for fat, protein, and lactose, and 0.12% for total solids, when instrument results are compared with results of specified AOAC  methods. Recommended maximum mean differences between instrument and standard results are based on 95% confidence limits for the mean of 8 samples; precision and systematic errors are calculated as the standard deviation of difference between duplicates for 8 samples, and systematic errors are calculated as the standard deviation of difference between instrument and reference results for 8 samples. Automated sections for milk of Official Methods of Analysis were rewritten to eliminate references to individual instruments, to eliminate possible differences between calibration procedures for different instruments, and to stress important instrument operational characteristics which can have significant effects on instrument performance.