Fractionation of human liver mitochondria: enzymic and morphological characterization of the inner and outer membranes as compared to rat liver mitochondria.

The fractionation of human liver mitochondria into inner membrane, outer membrane and matrix material is reported. Compared with rat, human liver mitochondria are more fragile. Fractionation can be achieved in only 2 steps, a digitonin treatment for removal of the outer membrane and centrifugation of the inner membrane plus matrix particles through a linear sucrose gradient resulting in purified inner membranes and matrix.


Controlled cell disruption: a comparison of the forces required to disrupt different micro-organisms.

A cell disrupter has been developed which can measure the forces required to disrupt both eukaryotic and prokaryotic cells. It operates a continuous process and will disrupt both large and small volumes. Shear forces are set up when a suspension under laminar flow conditions is released under high pressure through a short orifice. If the applied pressure is altered, the shear forces are simultaneously changed so that the amount of cell disruption can be compared under different known and repeatable conditions. The disrupter is now manufactured and supplied by Stansted Fluid Power Limited, Stansted, England. Phase-contrast microscopy has shown that the disrupter will break a variety of organisms including Chlorella, Aspergillus fumigatis, Fusarium sp., Saccharomyces cerevisiae, Escherichia coli, Lactobacillus casei, Bacillus subtilis, Clostridium perfringens, Streptococcus faecalis, Streptococcus zooepidermicus and Staphylococcus aureus. The cells are not all broken at one pressure but a certain pressure must be applied before disruption starts which will then increase rapidly as the applied pressure is increased. The applied pressure required to disrupt half the population in a culture is different from one species to another, rods being disrupted more easily than spheres. The case of disruption seems to be related to the shape and chemical composition of the cell wall. Furthermore, the disrupting process, in an unsynchronized culture is not random and may be related to the statistical size distribution of the cells.


Complementation of G1-phase variants of a mammalian cell cycle.

Complementation between temperature-sensitive (ts) variants of Balb/c-3T3 defective in the G1 phase of its cell cycle was measured in the [3H]thymidine-labeling indices of the multinucleated cells during incubation at the restricted temperature (38 degrees C) following cell fusion. One ts variant from each group along the length of the G1 phase was tested for complementation. Varying degrees of complementation were observed between the 4 ts variants tested, judging by the time of entry into S-phase and the degree of synchrony attained. At least 3 complementation groups were discernible.


Three-dimensional reconstruction of the chromatin bodies in the nuclei of mature erythrocytes from the newt Triturus cristatus: the number of nuclear envelope-attachment sites.

The arrangement of the chromatin bodies in the interphase nuclei of 6 erythrocytes has been investigated by means of 3-dimensional reconstruction from electron micrographs of serial sections. When the borders of chromatin bodies are marked on the surface of each model, discrete areas of chromatin in contact with the nuclear envelope are revealed. The number of these areas in approximately equal to the number of chromosomes in the diploid set. The data suggest that each chromatin body corresponds to a condensed interphase chromosome and that each chromosome is attached to one discrete site on the nuclear envelope. The data are insufficient to show whether or not the condensed chromosomes are arranged in any orderly pattern in these nuclei.


The quantitative measurement of electrolyte elements in nuclei of maturing erythrocytes of chick embryo using electron-probe X-ray microanalysis.

Na, K and Cl measurements have been made on frozen sections of chick red blood cells throughout embryonic development, using electron-probe microanalysis. There is an apparent fluctuation in the levels of these elements during maturation, although the Na/K ratio remains fairly constant. The nuclear Na concentration resembles that of the cytoplasm, rather than that of the medium, at all stages. Inhibitor studies indicate that when cytoplasmic Na, K and Cl levels are altered, their corresponding nuclear levels are similarly affected. Additionally, the measurements in nuclei isolated in anhydrous media from lyophilized cells have shown artefactual accumulation of high Na, K, Ca and Mg.


Serum myoglobin level in altered thyroid states.

Serum myoglobin (Mb) levels were measured in normal, hyperthyroid, and hypothyroid subjects by RIA. Serum Mb levels were significantly higher (P less than 0.001) in untreated hypothyroid patients and significantly lower (P less than 0.01) in untreated hyperthyroid patients than in normal subjects. There was a significant inverse correlation (P less than 0.01) between serum T4 or T3 levels and Mb levels in these subjects. Serum Mb levels were normalized after correction of the abnormal thyroid states.


Circulating melatonin in man: episodic secretion throughout the light-dark cycle.

A sensitive and specific RIA for melatonin has been validated for human plasma. Five young adult men had plasma samples obtained every 20 min during two 24-h periods. One was a normal active period and the other a basal period with complete bed rest. Melatonin was found to be secreted episodically throughout the 24 h in each condition, with secretory episodes clearly present during the waking day in the presence of bright light.


Danazol administration to females with menses-associated exacerbations of acute intermittent porphyria.

Acute intermittent porphyria (AIP) is a disorder of porphyrin metabolism in which the basic defect is a partial deficiency of uroporphyrinogen I synthase. The clinical disorder is more common in women, and some experience acute attacks before menstrual periods. Oral contraceptives have prevented menstrual-associated attacks in some cases, but exogenous estrogens and progestins are otherwise contraindicated in this disease. Danazol, a new synthetic steroid with weak androgenic activity, was thought to be of potential therapeutic benefit in AIP because of its effect of decreasing gonadotropin secretion without exposure to estrogen or progesterone. The drug was administered at a dosage of 200 mg t.i.d. to two adult females with AIP who were experiencing frequent exacerbations of their disease in association with their menstrual periods. Symptomatic and chemical evidence for exacerbation of porphyria occurred within 10 days of commencing danazol treatment in both patients.


Hyperglycemia and glucagon suppression: possible importance of the vagus and enteric humoral factors.

Augmentation of insulin release after oral glucose by a gastrointestinal humoral mechanism is well accepted. The suggestion of a similar mechanism for suppression of glucagon release after oral glucose has not been previously tested. In this study, plasma glucagon levels have been estimated in five normal subjects after both oral and iv administration of glucose. A variable iv glucose infusion rate with frequent monitoring of blood glucose was used to match the hyperglycemia produced by the 50 g oral glucose and the iv glucose loads. Virtually complete suppression of plasma glucagon levels was seen in both cases (nadir of glucagon levels 16 +/- 6 pg/ml for oral glucose; 11.4 +/- 3 pg/ml for iv glucose). Thus, enteric humoral factors did not facilitate glucagon suppression after oral glucose ingestion in man. The vagus nerve is also involved in mediating the alpha-cell response to hypoglycemia and, thus, to examine whether hyperglycemia suppresses glucagon release through a vagal mechanism, iv atropine (15 microgram/kg) was given 20 min before administration of oral or iv glucose. Atropinization delayed the glucagon suppression after oral glucose, but this delay was probably related to delayed glucose absorption from the gut. With iv glucose, atropinization did not affect the degree of suppression of glucagon levels. It is concluded that alpha-cell suppression in response to hyperglycemia is not mediated via the vagus.


Blood pressure response to an angiotensin II antagonist in patients with acromegaly.

The renin-angiotensin-aldosterone system in patients with acromegaly was evaluated by infusing [sarcosine1, isoleucine8]angiotensin II, a competitive angiotensin II antagonist, into five acromegalic patients with hypertension and three normotensive acromegalics. The drug was infused at a rate of 600 ng/kg . min for 30 min, 1 h after iv injection of 40 mg furosemide. In addition, before the infusion, plasma samples were obtained for determination of PRA and plasma aldosterone concentration. A significant pressor response to [sarcosine1, isoleucine8]angiotensin II was observed in all eight patients. Preinfusion PRA and plasma aldosterone concentration were significantly lower than in normal controls. It is concluded that in acromegaly, the renin-angiotensin-aldosterone system is suppressed and that this system is probably not involved in maintenance of the high blood pressure observed in some acromegalic patients.


Hyperglycemia per se (insulin and glucagon withdrawn) can inhibit hepatic glucose production in man.

We examined the effect of hyperglycemia per se on net splanchnic glucose balance. In 2 groups of normal postabsorptive men who had undergone hepatic vein catheterization, somatostatin was administered to block endogenous insulin and glucagon secretion. Exogenous glucose was infused in both groups to maintain euglycemia for 2 h in one group (n = 7) and to induce hyperglycemia of 220-240 mg/dl after 30 minutes of euglycemia in the second group (n = 4). In both groups the induction of insulinopenia and glucagonopenia with euglycemia maintained resulted in an initial 75% fall in net splanchnic glucose production (NSGP). In the group in which euglycemia was maintained NSGP returned to basal rates (157 +/- 31 mg/min) within 2 h. However, in the group in which hyperglycemia was induced, NSGP did not return to basal rates but remained suppressed (28 +/- 4 mg/min) for the duration of the study. These data in normal man indicate that hyperglycemia per se with insulin and glucagon acutely withdrawn can suppress splanchnic glucose production but does not induce net splanchnic glucose storage.


Ectopic human growth hormone in ovaries and breast cancer.

Immunoreactive human GH (hGH) has been found in 8 of 118 surgically removed ovaries, with concentrations ranging from 50-51,000 ng/g tissue. The highest concentration was in an ovarian metastasis from an adenocarcinoma of the breast; a skin metastasis from this same carcinoma had an hGH concentration of 350,000 ng/g. All specimens were positive for hGH by radioreceptor assay as well as by RIA. Examination of four specimens at multiple dilutions by lactogenic and somatotropic receptor assays as well as by RIA did not reveal significant differences in slope between these specimens and that of the hGH standard. Sephadex gel chromatography of two specimens showed the major peak of immunoactivity eluting coincident with monomeric hGH. Ectopic hGH has not previously been reported in ovary or breast.


Metabolic clearance and plasma half-disappearance time of exogenous somatostatin in man.

The MCR and half-disappearance time of exogenously administered somatostatin have been measured during and after cessation of a constant infusion. Studies were performed on normal volunteers and patients with chronic liver disease and failure. Immunoreactive somatostatin was measured by a sensitive and specific RIA using an antiserum directed against the core of the molecule. Normal subjects had a mean MCR of 1949 +/- 250 ml/min (28.4 +/- 4.2 ml/min . kg BW) (mean +/- SEM), similar to values found in five patients with chronic liver disease. However, patients with chronic renal failure showed a highly significant (P less than 0.001) lowering of the MCR (501 +/- 32.7 ml/min or 7.8 +/- 0.6 ml/min . kg). The rate of disappearance of somatostatin after infusion was linear for 7-10 min, after which a much slower component was observed. In normal subjects, the t 1/2 of the first component varied from 1.1-3.0 min, in patients with liver disease it varied from 1.2-4.8 min, and in patients with chronic renal failure it varied from 2.6-4.9 min. Exogenously administered somatostatin is rapidly cleared in normal subjects and patients with chronic liver disease, but the MCR in end stage chronic renal failure is markedly lowered. The kidney may have a role in the metabolic clearance of exogenously administered somatostatin, or uremia may impair catabolism nonspecifically.


Acromegaly associated with a bronchial carcinoid tumor: evidence for ectopic production of growth hormone-releasing activity.

A patient with acromegaly, pituitary enlargement, and elevated plasma GH levels also had a bronchial carcinoid tumor. Signs and symptoms of active acromegaly along with elevated GH levels persisted for 11 yr after hypophysectomy and pituitary stalk section. Resection of the bronchial carcinoid reduced plasma GH to barely detectable levels. Extracts of the frozen carcinoid tumor were devoid of significant GH, but when added to isolated pituitary cells of estrogen-primed male rats in 4-day primary culture exhibited specific GH-releasing activity in vitro. These findings strongly suggest that the patient's acromegaly resulted from continual stimulation of pituitary somatotrophs by a GH-releasing factor secreted by the bronchial carcinoid.


A pregnancy in an acromegalic woman during bromocriptine treatment: effects on growth hormone and prolactin in the maternal, fetal, and amniotic compartments.

An unexpected 20-week-old pregnancy was found  in a young acromegalic who had been treated with 10 mg bromocriptine/day for 10 months. The drug was continued throughout the period of gestation. No growth of the pituitary adenoma was noticed. The intrauterine development of the fetus was normal. Bromocriptine therapy had no discernible effect on the expected patterns of secretion of placental hormones, but inhibited completely the increase of PRL in the serum of the mother. Maternal plasma GH concentrations were very high in spite of the treatment and progressively declined after delivery. The plasma GH level was normal in the child, but PRL was very low at birth and increased in the following days. The expected high PRL concentration was found in the amniotic fluid. This case study suggests that bromocriptine crosses the human placenta and affects the fetal pituitary, maternal GH does not influence fetal or amniotic GH, and amniotic fluid PRL correlates poorly with either maternal or fetal blood levels and is not affected by bromocriptine.


In vitro inhibition of renin by human des-angiotensin I renin substrate.

Human des-angiotensin I renin substrate, a cleavage product of the renin reaction, was generated in plasma and from semipurified renin substrate by exhaustive incubation with renin and was quantitated by a direct RIA. An increase in the Michaelis-Menten constant of the renin-substrate reaction, from 2000 to 5000 ng angiotensin I equivalents of renin substrate/ml, with no change in maximal velocity was observed upon addition of this semipurified protein to plasma. It is suggested that this phenomenon of competitive inhibition of the renin reaction by des-angiotensin I renin substrate accounts for discrepancies in the determination of renin substrate concentration and possibly has physiological significance for feedback inhibition of angiotensin I generation.


Longitudinal post-validation of the MMPI Escape (EC) and Prison Adjustment (AP) scales.

Conducted a longitudinal 3-year post-validation study of the MMPI Escape (Ec) and Prison Adjustment (Ap) scale score of 301 male felon inmates convicted of major type crimes and serving sentences of 5 years or more within the North Carolina Correctional System. Examination of the escape and infraction records of these inmates in relationship to their Ec and Ap scale scores demonstrated that the two scales were still effective classification instruments in predicting escape from within prison units that provided inner and perimeter security and in predicting the committing of major rule infractions irrespective of the degree of custodial control. Caution was expressed concerning the use of the Ec and Ap scale scores without supportive date from their classification procedures.


Comparison of the utility of two abbreviated forms of the MMPI for psychiatric screening of the elderly.

Assessed the utility of the Faschingbauer Abbreviated MMPI (FAM) and MMPI-168 for use with the elderly. FAM and MMPI-168 items were extracted from the MMPIs of 24 older male veteran medical or alcoholic patients. The results indicate that both abbreviated forms are highly correlated with and yield similar diagnostic inferences to the standard MMPI. For this sample population, the FAM was significantly (p less than .001) more in accordance with the standard MMPI than the MMPI-168.


Note on the relationship between trait anxiety and the Eysenck Personality Questionnaire.

Administered the State-Trait Anxiety Inventory A-Trait scale and the Eysenck Personality Questionnaire to 97 females and 105 males under neutral conditions. The interrelationships gave limited support to Eysenck's hypothesis that anxiety is a dimension oblique to extraversion and neuroticism such that high anxiety is associated with high introversion and neuroticism. Trait anxiety was independent of the psychoticism and lie dimensions.


Utility of the WAIS in predicting vocational success of psychiatric patients.

Examined the utility of the WAIS OA, BD, PIQ and FSIQ scores in combination with age, apparent degree of emotional severity and psychiatric disability in predicting the vocational success of 180 psychiatric outpatients. Although no significant statistical differences were obtained in a cros-validation procedure indicating that psychiatric disability affected the efficacy of the derived regression equations, substantial differences in predictable variance accounted for were found to be related to the nature of the psychiatric disability. This must be attended to in attempts to predict the vocational success of psychiatric outpatients.


Haptic visual discrimination and intelligence.

Investigated the relationship between tactual-visual discrimination and intelligence from a neuropsychological perspective. Parieto-occipital areas were conceptualized as mediating centers for the integration of tactile and visual information, as well as for the processing of higher cortical functions. A recently designed test of tactual-visual information processing, the Haptic Visual Discrimination Test (HVDT), was administered to a group of 39 first-grade school children. Standard tests of intelligence (Wechsler Intelligence Scale for Children-Revised, WISC-R), academic potential (Metropolitan Readiness Test, MRT), and spatial integration (Bender Visual Motor Gestalt Test, BVMGT) also were given. The obtained correlation coefficients revealed consistently significant associations between the importance of parieto-occipital areas of the cortex for organizing sensory data as well as for the processing of intellectual information.


The use of contingent reinforcement for improving the personal appearance and hygiene of chronic psychiatric inpatients.

Chronic psychiatric patients were Ss in a study designed to evaluate the effectiveness of contingent reinforcement to improve personal appearance and hygiene. Ss were divided into four groups matched on age and years of hospitalization. Group 1 was rated on personal appearance and received contingent material reinforcement and verbal reinforcement. Group 2 was rated and received only contingent verbal reinforcement. Group 3 was rated, but received no contingent reinforcement, and Group 4 was control. It was concluded that (1) both contingent verbal reinforcement and contingent material plus verbal reinforcement can be used to improve personal appearance; (2) contingent material plus verbal reinforcement is more effective for improving personal appearance than using only contingent verbal reinforcement; (3) rating patients without using contingent reinforcement does not result in significant improvement; and (4) simply presented video-taped information for improving personal appearance does not cause significant improvement.


Locus of control and therapists' occupational role as determinants of clinical prognoses.

Clinical studies that used the internal-external locus of control model developed by Rotter (1967) have shown a marked preference among clinicians for clients with an internal locus of control. In the belief that occupational roles can affect such preferences, this study examined clinicians' (sychiatrists, psychologists, social workers) prognoses for clients who had described their problems in terms of either an internal, external, or no locus of control. As hypothesized, a 5 X 3 X 3 replicated Latin Square design found that while clients who attributed their problems to an internal locus of control were given better prognoses, clinicians differed along occupational role dimensions in their prognoses for the same client. The implications of those occupational differences for professional practice were discussed.


Clinical follow-up: treatment and outcome of functional headache patients treated with biofeedback.

Sixteen diagnosed functional headache patients treated with biofeedback techniques of frontalis EMG feedback and/or peripheral temperature feedback, with an average headache history of 18.2 years, were evaluated at an average interval of 37 weeks posttreatment. On the average, patients showed further improvement in their headache status as measured by average daily headache pain scores in contrast with termination values. Data were obtained via structured interviews conducted by an individual not involved in patient's treatment. Patients on the average showed declines in frontalis EMG during the follow-up sessions, but not increases in temperature as had been expected. The unclear role played by increasing peripheral temperature in follow-up of migraine patients is discussed.


Death anxiety: "state" or "trait"?

Investigated relative effects of hypnosis, alpha biofeedback, prestige suggestion, and silence in attenuating experimentally induced increases in death anxiety. Forty female undergraduate Ss at Louisiana State University were tested on four measures of death anxiety: "Emotional" associations to "death" words, association response latencies to "death" vs. "neutral" words, Death Anxiety Scale, and Death Concern Scale. Ss then were assigned to four treatments: (1) Hypnosis, with anxiolytic post-hypnotic relaxation suggestions; (2) Nonhypnotic anxiolytic prestige suggestions; (3) EEG alphacontingent biofeedback; and (4) a 15-minute waiting period. After treatments, Ss viewed a tape-slide presentation that emphasized personal death and overestimated its probability from various causes. Ss then were retested on death anxiety measures, forms of which were counterbalanced within groups. Analysis of variance (at .05 and .01 confidence levels) failed to differentiate groups on either increases or decreases of death anxiety. It was concluded that death anxiety may be a "trait" as opposed to a "state" phenomenon.


Behavioral treatment for depression: an evaluation of therapeutic components.

Designed study to determine the "active ingredients" in Lewinsohn's behavioral treatment for depression. Fifty-six men and women were screened for significant depression and were assigned to four experimental treatment groups: (1) an activities-increase group instructed to increment participation in reinforcing activities; (2) an expectancy-control group that participated in a fitness program; (3) a self-monitoring control that simply recorded activities and mood daily; and (4) an attention-control group. Consistent with an operant model of depression, the resulted indicated that increases in activities that Ss had rated as pleasureable alleviated depressed mood over a 30--day time period. While all Ss evidenced some improvement in mood, the activities-increase group showed significantly greater improvement than Ss in groups that did not increase their activity levels. Correlational analyses of daily activity and Depression Adjective Check List Scores proved additional support for the behavioral model of depression. The outcome for the four experimental groups was not different on a measure of belief in internal vs. external control of reinforcement.


The pupillometric response as a parameter of self-esteem.

Investigated the relationship between self-esteem and the pupillometric response. Because a positive correlation has been established between pupillary dilation and positive interest in an observed stimulus, it was hypothesized that self-esteem would be correlated positively with the pupillometric response when Ss (N = 82) viewed photographs of themselves. Results indicated that self-esteem was related to the pupillometric response only for females. The relationship, however, was opposite to that predicted. The greater the pupillometric index, the poorer the self-esteem. It is suggested that in a situation in which implicit anxiety states are involved, sympathetic nervous system activity predominates over the interest value of the stimulus. It may be that females more than males are constitutionally prone to exhibit this sympathetic reaction and/or are more responsive to the body image properties inherent in the stimulus photographs.


The bias in psychotherapy research evaluation.

Using a 3 X 3 X 3 factorial design, clinical psychologists' (N = 204) evaluations were obtained of the methodology of a bogus psychotherapy outcome experiment that varied on the outpatient population treated and the results. Three types of neurotic outpatients were treated in the bogus study, and the experiment's results indicated that psychoanalytically oriented therapy was more effective than behavior therapy, behavior therapy was more effective than psychoanalytically oriented therapy, or psychoanalytically oriented therapy and behavior therapy were equally effective. The third independent variable was psychologists' theoretical orientation (psychodynamic, behavioral, or eclectic). A significant Orientation X Results interaction was obtained, and the results suggest that psychodynamic clinicians are biased against outcome research that demonstrates the superiority of behavior therapy.


Dependence, relapse and extinction: a theoretical critique and a behavioral examination.

Re-examines the "decay of extinction" notion proposed by Hunt and Matarazzo (1970) to account for relapse in the treatment of alcoholism and dependence disorders. The attempt to reduce complex relapse behaviors to a simple curve is criticized both from the point of view of using group data to describe an individual process and the nature of cumulative data itself. Because individual differences are marked, a simple group curve cannot describe the process of relapse. A behavioral examination then is presented, based on records of the drinking behavior of 79 alcoholics over a 12-month period after treatment. The main finding is that there are marked individual differences in the process of relapse. Furthermore, the frequency of heavy drinking seems to be most related to a pattern of drinking characterized by marked month-to-month variation in the frequency of heavy drinking rather than a more stable pattern.


Application of discriminant analysis to level of performance of alcoholics and nonalcoholics on Wechsler-Bellevue and Halstead-Reitan subtests.

Investigated the utility of subtests from the Wechsler-Bellevue Intelligence Scale and the Halstead-Reitan Neuropsychological Test Battery to differentiate between alcoholics and nonalcoholics (N = 76). Analyses of variance indicated that the alcoholics were more impaired than nonalcoholics. It was found that the Wechsler-Bellevue Performance subtests were more discriminative than were Verbal subtests, with performance on the Halstead-Reitan variables being intermediate. The nine measures that differentiated most significantly between groups were subjected to a stepwise multivariate discriminant analysis. The resulting function correctly classified Ss with an overall accuracy of 74.7%. It was found that the Block Design subtest was the best single discriminator. The findings were discussed in relationship to previous findings and with respect to general issues of clinical neuropsychological assessment.


Alcoholism, sex-role orientation and psychological distress.

Distribution of scores on the Bem Sex Role Inventory obtained from 69 randomly selected alcoholics was found not to differ significantly from that reported in normative data (Bem, 1974). Sexually androgynous alcoholics (N = 21), those able to affirm about themselves characteristics ordinarily attributed to both males and females, scored significantly lower on MMPI scales D, PD, PT, and SI and and higher on MA than a nonandrogynous group (N = 21) of alcoholics. The results were discussed in terms of alcoholics varying along psychopathological and nonpsychopathological scales in a manner similar to "normal" Ss.


An attitude survey of the effects of marijuana on sexual enjoyment.

Determined attitudes on the effects of marijuana on sexual enjoyment by self-report for a group of 84 graduate students of health sciences. The students were grouped in three categories: those who had sexual experience while under the influence of marijuana (experienced smokers), those who have smoked marijuana but who have not had such experience (non-experienced smokers), and non-smokers. Results are again inconclusive despite the fact that a majority in each category responded in a positive manner to the initial question concerning the effect of marijuana on the enjoyment of sexual intercourse. There is sufficient support to indicate that at least some experienced smokers have derived an enhancement of sexual pleasure while they were using marijuana. The implication is that there may be value in researching the use of marijuana in treatment of sexual disorders.


Training, role models, and research activity among clinical psychologists.

Compared clinical psychologists (N = 270) with high and low publication rates through questionnaire responses. Highs report dissertation experiences more positively influenced their research motivation. Although the most influential professor in both groups was typically clinical, more highs found the professor emphasized another orientation, particularly empirical research, over clinical expertise. Highs report more time available, collaboration opportunities, and organizational acceptance for their research. Both groups favor empirical methods, although highs and lows agree that a small proportion of psychological research makes a significant contribution. Individual preferences probably interact with situational characteristics, both in training and work settings, to determine research activity.


Follow-up research in primary prevention: a model of adjustment in acute grief.

Investigated the effects of preventive intervention that followed the life crisis of sudden death in the family. Two bereaved groups of families (one of which received preventive intervention service) and one non-bereaved group were compared in an outcome design and were assessed for indices of illness, psycho-social disturbance, and general quality of life. The results revealed that sudden death has a two-stage impact upon family survivors and that subsequent adjustment can be predicted from a knowledge of facts at the time of death. A preventive intervention service had little or no impact and may have been harmful. Discussion centered on possible intervention strategies with a focus upon the complex determinants of environmental stresses and family/individual variables.


Neuropsychological impairment with schizophrenia vs. acute and chronic cerebral lesions.

Performed detailed neuropsychological evaluations with 25 recently hospitalized schizophrenics in whom systematic neurologic workups had failed to reveal CNS disease. Efforts were made to minimize possible effects of drug-induced extrapyramidal symptoms on test performance. Although the schizophrenics showed some neuropsychological impairment relative to 25 normals, their deficits were not as severe as those of patients known to have either acute or chronic brain disorders (N = 25). The diagnostic accuracy achieved by the tests supports their use in short-term treatment facilities when the differential diagnosis includes schizophrenia and brain disorder, especially acute brain disorder. Schizophrenics' neuropsychological impairment was more correlated with degree of EEG abnormality than with degree of psychosis, which suggests a possible organic basis for the deficits that they showed on testing.


Neuropsychological test performances of paranoid schizophrenic and brain-damaged patients.

Predicted that simple tests that require attention and motor speed would be able to differentiate paranoid schizophrenics from brain-damaged patients better than more complex, problem-solving neuropsychological tests. The strategy was to improve discrimination between schizophrenic and brain-damaged patients by selecting a schizophrenic subgroup with a recognized cognitive strong point. Fourteen organic, 14 paranoid schizophrenic, and 14 nonpsychotic psychiatric patients matched for sex, education, and IQ were tested. As predicted, the attention and speed measures differentiated the brain-damaged and paranoid groups, while the four more complex measures from the Halstead-Reitan Battery did not separate the groups. There were no significant differences between the two psychiatric groups.


Autonomic components of vicarious conditioning and psychopathy.

Examined vicariously instigated electrodermal activity within a classical conditioning paradigm using electric shock to a model as the unconditioned stimulus. Thirty-nine male Ss were selected for the study and divided into three groups: primary psychopaths (PP), secondary psychopaths (SP), and nonpsychopathic normals (NP). The selection procedures were based on scores obtained from the MMPI and the Activity Preference Questionnaire. The conditioning paradigm was composed of three GSR recording periods, which were identified as resensitization, conditioned anticipation, and vicarious instigation, respectively. Results indicated that the PP and the SP groups produced significantly less conditioned anticipatory GSRs than the NP group. The PP group also produced significantly less vicariously instigated GSRs than the SP and NP groups.


The early identification of anxiety-laden material with the aid of skin conductance measurements.

Measured electrodermal responses (EDR), in the form of changes in skin conductivity, during administration of a calibration questionnaire (CQ) and a personal history questionnaire (PHQ) to each of 25 Ss. "Strong" changes were defined statistically for each S from the amplitudes of EDR evoked by the CQ. The free-floating anxiety of each S also was measured. As predicted on the grounds that questions of the PHQ were more likely than the non-personal questions of the CQ to intersect unresolved, anxiety-laden material, it was found that strong EDR evoked by the PHQ were significantly more likely to perseverate than those evoked by the CQ. Hence, the technique has potential clinical use in identifying anxiety-laden material. Free-floating anxiety did not correlate significantly with EDR data.


Stepwise regression of psychosocial problems on global impairment ratings.

Calculated the correlations between the severity ratings for 27 psychosocial problems and global impairment ratings of daily functioning for 109 men and 206 women admitted to the outpatient services of a community mental health center. Controlling for sex and total number of problems rated, a stepwise regression analysis indicated that global impairment estimates were related to problems that represented thought processes, affect, and role functioning. The severity ratings for the thought process problems contributed the most variance (14%) toward the explanation of the global impairment ratings.


Dimensionality of the repression sensitization scale.

Study of the internal structure of the R-S Scale failed to support a finding of unidimensionality or bipolarity, as theory suggests. A factor analysis of the item matrix revealed a poorly defined multifactor structure that contained five relatively weak unipolar factors (N = 200). Guttman's Reproducibility and the Kuder-Richardson Coefficient adjusted for test length similarly failed to support the presence of a unifactor structure. The relatively high Coefficient of Dimensionality was rejected as evidence of unidimensionality given the uncertainty over what psychometric properties this index reflects. The high proportion of S-item interaction variance showed considerable equivocal item content, which suggested marked error factors due to Ss' idiosyncratic interpretation of items.


MMPI correlates of looking left or right during mental tasks.

Correlated directionality of eye movements during mental tasks with MMPI profiles of 22 women and 16 men evaluated for mild neurotic disorder. Directionality of eye movements was correlated with MMPI scales at above chance levels, which suggests that the phenomenon of eye movement directionality relates to objective measures of personality.


An inventory for the identification of child abusers.

Conducted a literature review to identify personality traits that are characteristic of individuals who abuse and neglect children. On the basis of reported traits, a Child Abuse Potential Inventory that consisted of 334 items was constructed. The Inventory was administered to 19 abusing and 19 matched nonabusing parents. An item analysis was conducted to identify those items that discriminated between abusers and nonabusers. While the best 7 items accounted for slightly over 90% of the variance, the top 25 discriminators accounted for 99% of the variance. A principal axis factoring with oblique promax rotations was performed. Four factors, or dimensions, were identified. They were: loneliness, rigidity, problems and control. Additional analysis indicated that rigidity and problems dimensions were the best predictors of abuse.


Rapid establishment of therapeutic serum concentrations of salicylates.

A minimum serum salicylate concentration of 150 microgram/ml is required to control certain inflammatory disease processes. A loading regimen designed to rapidly achieve this minimal level was evaluated in six normal volunteers (age 22 to 27 years, weight 70.5 to 84.1 kg) using a randomized crossover design. The control group received 650 mg aspirin (ASA) every 4 hours for 48 hours. The loading regimen was 2600 mg ASA divided into two equal doses 4 hours apart. Maintenance dosing of 650 mg ASA every 4 hours was then started 4 hours after the completion of the loading regimen and continued for 40 hours. Serum samples were drawn at 0, 2, 4, 6, 8, 12, 24, 36, and 48 hours after initiation of the study and were assayed for salicylate concentration by UV spectrophotometry. Loading with aspirin produced serum concentrations which were significantly higher (P less than 0.01) for the first 24 hours and reduced the time to reach 150 microgram/ml (15.3 +/- 5.9 hours versus 30.4 +/- 8.65 hours, P less than 0.001) for five of six subjects when compared to a conventional regimen. One subject did not achieve 150 microgram/ml at 48 hours with either regimen. Considerable intersubject variation in serum concentration was noted at 48 hours for both regimens. We suggest that a loading regimen for aspirin may have utility for patients in whom rapid attainment of a therapeutic antiinflammatory serum concentration is desirable.


Withdrawal syndrome following cessation of guanabenz therapy.

A withdrawal syndrome consisting of nervousness, palpitations, diaphoresis, and insomnia appeared in three patients within 16--48 hours following discontinuation of guanabenz, a new centrally acting antihypertensive agent. A similar syndrome of sympathetic overactivity has been described with abrupt withdrawal of clonidine. Three of four patients treated with 48 mg/day but none of 20 other patients treated with 32 mg/day or less guanabenz developed this syndrome. None of the three patients developed hypertensive crisis, though one had a modest rise in the blood pressure above baseline levels. It is concluded that guanabenz therapy should not be discontinued abruptly and that, when possible, the dosage should be limited to less than 48 mg/day.


The overdosage and/or poisoning information gap.

The availability of poisoning information from the pharmaceutical industry was evaluated. Manufacturers of the top 50 drugs were surveyed. Information that was received was classified by established criteria for evaluation. Compendia on poisoning information was evaluated based on 11 components ranging from toxicity information to information available in the PDR. Additional information on emergency telephone numbers for after-hours service was accumulated. The results showed that much of the information available is incomplete and/or outdated. A concerted effort to establish a central source for manufacturers' poison information needs to be begun.


Communication therapy for the young adult.

Speech clinicians are often frustrated in their assessment and therapeutic management of a young adult with speech and language problems. Procedures for children with language disorders and aphasic adults are often inadequate for the adult who has communication impairments that cannot be attributed to aphasia. This case study presents an account of a 29-year-old woman who had cognitive, linguistic, and pragmatic difficulties. Both diagnosis and therapy were based on recent innovations in the areas of cognition, linguistics, and interpersonal relations. Results indicated that therapy was successful when the client was responsible for therapy activities, when therapy combined tutorial as well as experimential sessions, and when each session focused on the development of cognitive, linguistic, and interpersonal domains. Specific examples of diagnostic and therapeutic activities are presented as well as the rationale underlying the approach to therapy.


The operant training and generalization of pronouns and a verb form in a language delayed child.

The purpose of the present investigation was to train the correct use of three pronouns and the verb from "are" in a language delayed child and to assess the generalization of these trained responses to untrained stimuli. Within a multiple base-line design the four selected behaviors were sequentially trained using the techniques of positive reinforcement, modeling, and punishment for inappropriate responses. At succeding stages of training, probe trials were conducted to assess the extent of generalization of trained responses to untrained stimuli. A 100% generalization of trained responses to untrained stimuli was obtained for each of the four linguistic behaviors.


Use of mean morphological units to assess language development.

The intent of this study was to determine what size language sample was needed in order to obtain a reliable index of mean morphological units. Shriner (1967) suggested that 50 sentences were necessary for obtaining a mean length of response and a length complexity index. Brown (1973) recommended using 100 sentences when determining mean morphological units. However, other investigators (Schneiderman, 1955; Griffith and Miner, 1969) said that as few as 15 sentences could serve to provide enough data for reliable estimates of mean length of response and a length complexity index. For a practitioner, knowing that the first 15 sentences produced would be a reliable estimate of overall language ability would be of substantial benefit. Obtaining, transcribing, and analyzing 15 sentences takes about 20 min. The results of this study show that as few as 15 sentences are adequate for determining a reliable index of mean morphological units.


The perceptual identity and characteristics of aging in normal male adult speakers.

Twenty untrained male and 20 untrained female listeners were requested to judge the age of 46 male speakers from samples of conversational speech.  Thirty-seven samples were judged similarly by the two groups and were therefore provided as listening stimuli for ten male and ten female speech pathologists. Through an a posteriori schema, speech pathologists were required to specify the salient features of speech judged as being characteristic of each speaker's perceived age. The results of the study indicated that untrained listeners used similar perceptual sets to judge age until after the fiftieh year of life. Regardless of sex, listeners tended to underestimate the age of the speakers. Male and female speech pathologists viewed low pitch and hoarseness as being the most salient features of speech for the speakers. Qualitative analysis revealed that the most prominent features indicated by speech pathologists could be classified according to pitch, quality, articulation, and rate of speech.


Judging personality and appearance from voice disorders.

This study examined the effect of voice disorders on judgments of personality and appearance. One-hundred-and-five college students evaluated the personality and appearance characteristics of 12 speakers, four with normal voice quality, four with harsh-breathy voice quality, and four with hypernasal voice quality. A semantic differential procedure was employed to obtain ratings on 12 bipolar adjectives. Results indicate a significant difference between the normal and disordered voices. Voice disorders elicit more negative responses on judgments of personality and appearance of the speaker. These findings indicate that voice disorders warrant correction.


Time couse of the blockade effect of propranolol on sinus node and atrioventricular node.

The time course of the blockade effect of propranolol on the sinus node (SN) and the atrioventricular node (AVN) was studied in six normal volunteers. Serial isoproterenol infusions were done before and after oral propranolol administration, 160 mg daily for two days. The inhibition by propranolol of the heart rate increases due to isoproterenol was used to assess the blockade of the sinus node, and the diminution by propranolol of the shortening in the PR interval due to isoproterenol was used to assess the blockade of the atrioventricular node. The blockade effects on the sinus node and the atrioventricular node were identical and persisted more than 24 hours. There was no good relationship between plasma propranolol and blockade effect on sinus node and/or atrioventricular node as propranolol was no longer detectable in the plasma 24 hours after the last dose. A transient hypersensitivity to isoproterenol was present 36 to 48 hours after propranolol withdrawal. The explanation of these phenomena most likely lies in the peculiar nature of beta-adrenergic receptors.


Spectrographic and perceptual features of vocal tension/harshness in hearing-impaired adults.

Twenty hearing-impaired adult males exhibiting a range of severity of vocal tension/harshness sustained the vowels /a/ and /u/, at a constant intensity level and read the first two sentences of the Rainbow Passage. Recordings of the vowels and sentences were rated for degree of severity of vocal tension/harshness on a 5-point, equal-appearing-interval scale by three speech pathologists. Each vowel production was analyzed to produce a narrow-hand (10 Hz) frequency-by-amplitude acoustic spectrum in which the levels of inharmonic energy, i.e., noise components, were measured in dB SPL over the frequency range 100--3000 Hz. Significant correlations were obtained between: mean ratings of vocal tension for sentences and for vowels; mean ratings of vocal tension for vowels and average spectral noise levels for vowels; and mean ratings of vocal tension for sentences and average spectral noise levels for vowels.


Intelligibility in acquired dysarthria--a neuro-phonetic approach: three case studies.

A descriptive framework of phonetic parameters for the assessment of dysarthric speech is presented. The phonetic parameters examined are based on a linguistic analysis of aspects of continuous speech. The assessment thus evaluates the functional efficiency of the speech producing mechanisms in encoding the spoken medium of language. The interaction of the deviant parameters is related to the breakdown of intelligibility. Possible underlying neurological correlates are discussed. The implications for therapy using the combined phonetic and neurological information are considered. This assessment procedure is illustrated with case studies of three types of acquired dysarthria.


Tectal projections in the goldfish (Carassius auratus): a degeneration study.

Efferents revealed by degeneration staining following tectal lesions in goldfish are presented. Four major projections were found. Ascending ipsilateral projections to pretectal-diencephalic areas exit the tectum rostrally and laterally and terminate in the area pretectalis (AP), lateral geniculate (LGN), nucleus pretectalis (NP), and nucleus rotundus (NR). Ascending contralateral projections exit rostrally and possibly laterally, enter the posterior and postoptic commissures and terminate in the contralateral AP, LGN, NP, NR, and rostral tectum. A medially directed projection enters the intertectal commissure, and some of these fibers may terminate sparsely in an area of the contralateral tectum homotopic to the lesion. A descending projection exits the tectum laterally and projects ipsilaterally to a dorsolateral tegmental nucleus (DLT) and the lateral reticular formation of the tegmentum and pons, and contralaterally to the medial reticular formation of the tegmentum and pons.


The organization of the second somatosensory area (SmII) of the grey squirrel.

Microelectrode mapping methods were used to determine the organization of the second somatosensory representation, SmII, in grey squirrels. A systematic representation of the contralateral body surface was found in lateral parietal cortex adjoining the first somatosensory representation, SmI (Sur et al., '78a). The representation of the body in SmII was found to be much less distorted than in SmI. Under our recording conditions, almost all recording sites were activated from strictly contralateral body locations. The most important finding was that the basic orientation of the body representation in SmII is "erect" rather than "inverted." This orientation allows SmII and SmI to be adjoined along a common border representing the top of the head and face. This type of border has been called congruent (Allman and Kaas, '75; Kaas, '77), and it may have significance in the development of sensory representations.


Synaptogenesis in the cervical cord of the human embryo: sequence of synapse formation in a spinal reflex pathway.

Synaptogenesis in the cervical cord was studied by light and electron microscopy in human embryos ranging from four to seven weeks of ovulation age. The stage of embryonic development was estimated on the basis of external morphology of embryos and histology of the eye ball with reference to Streeter's horizon. No synapses were found in the cervical cord of the embryo at Streeter's horizon XIV (8 mm; estimated ovulation age, 28-30 days). A small number of axodendritic synapses appear in the motor neuropil of the cervical cord at Streeter's horizon XVII (14 mm; estimated ovulation age, 34-36 days). Since no primary afferents are demonstrated to reach the motor neuropil at this stage (the premotile period), these synapses are considered to be formed between interneurons and motor neurons. On the other hand, the formation of synapses outside the motor neuropil of the cervical cord was recognized at Streeter's horizon XX (22 mm; estimated ovulation age, 40-42 days), which corresponded to the period of onset of the precocious reflex, but not by horizon XIX (18 mm; estimated ovulation age, 38-40 days). The first axosomatic synapses were found in the motor neuropil at Streeter's horizon XVII (estimated ovulation age, 34-36 days). The present study suggests that the formation of synapses between interneurons and dendrites of spinal motor neurons precedes that of synapses between interneurons and collaterals of primary afferents. This sequence of synaptogenesis is in agreement with that reported in earlier studies with silver stain methods.


The environment of axonal migration in the developing chick retina: a scanning electron microscopic (SEM) study.

We have made a SEM study of the basal intercellular spaces of the retina in chick embryos of different developmental stages. Since this is the environment where optic axons grow, the structural characteristics of this region might play some role in the orientation of axonal migration towards the choroid fissure. The basal region of undifferentiated retinas is formed by the vitreal expansions of neuroepithelial cells. In pre-axonal stages, the intercellular spaces between these expansions do not show any preferential orientation towards the fissure. The growth cones of ganglion cell axons appear in an apicobasal direction and turn towards the fissure immediately beneath the vitreal surface. Fasciculation is an early event during development and, in the more advanced stages, the vitreal expansions from retinal cells are placed in rows following the same orientation as the axon bundles. These observations are discussed in relationship to current hypotheses on axonal migration and orientation.


The sensory component of the facial nerve of a reptile (Lacerta viridis).

The sensory fibers of the facial nerve in Lacerta viridis have been studied with a silver impregnation method to follow the course of axonal degeneration. Destruction of the geniculate ganglion demonstrated the degenerated sensory component of the facial nerve adjacent to the anterior vestibular root. Within the lateral vestibular area the facial sensory fibers consist of numerous rootlets separated by vestibular fibers and cells. These rootlets may join to form a main or paired sensory tract that passes through the vestibular nuclei to enter the tractus solitarius and divide into a small ascending prefacial component and a major descending prevagal division. A few fibers continue into the postvagal part of tractus solitarius and extend caudally to terminate in the nucleus commissura infima. Prefacial fibers terminate along the periventricular gray while prevagal fibers terminate within the tractus solitarius on the dendrites of cells of nucleus tractus solitarius and near the periphery of the dorsal motor nucleus of X. There was no noticeable degeneration in the descendens tractus trigemini. Terminal degeneration to descendens nucleus trigemini and motor nucleus of VII followed the tractus solitarius course. Most facial sensory fibers are probably related to taste and other visceral information.


Somatic neurons in the brainstem and neocortex projecting to the external nucleus of the inferior colliculus: an anatomical study in the opossum.

Some brainstem and neocortical neurons projecting to the external nucleus of the inferior colliculus were identified by retrograde axonal transport of horseradish peroxidase (HRP). Large pressure injections of HRP were used to define the population of cells in the dorsal column and trigeminal nuclei projecting to the entire inferior colliculus. Subsequently, tiny injections of HRP were delivered electrophoretically to various parts of the external nucleus using micropipettes. In each case the electrophoretic placements were made in sites identified as somatic by their response to foot shock or manual stimulation of the body. Receptive fields were found to be large and almost always bilaterally symmetric. Areas excitable by stimulation of the upper body were often found adjacent to areas excitable by stimulation of the lower body, tail, or, rarely, the face and head. In general, sites in the external nucleus which were identified by their responsiveness to stimulation of the body were not responsive to click stimuli. No topographic representation of the body was evident. After small electrophoretic injections of HRP absolutely restricted to the external nucleus, HRP-filled neurons were found bilaterally in the nucleus gracilis, nucleus Bischoff, (the midline and tail representation of the dorsal column nuclei), nucleus cuneatus, and nucleus trigeminalis, subnucleus caudalis. Ipsilateral neocortical cellular filling was found primarily in a continuous strip along the caudal and inferior margin of the parietotemporal cortex, in the area corresponding to SMII (Pubols, '77). Rarely, filled cells were found in the parietal area corresponding to SMI and in the temporal pole. In sum, small somatic-activated loci of the external nucleus receive bilateral, topographically convergent projections from hindbrain somatosensory structures and from the SMII cortex.


Projections from the spinal and the principal sensory nuclei of the trigeminal nerve to the cerebellar cortex in the cat, as studied by retrograde transport of horseradish peroxidase.

Projections from the spinal (Vsp) and the principle sensory (Vp) nuclei of the trigeminal nerve to the cerebellar cortex were studied by means of retrograde transport of horseradish peroxidase in the cat. Neurons projecting to the simple lobule and the dorsal part of the paramedian lobule (PMD) were located mainly in the dorsal part of the nucleus interpolaris (Vi) and of the caudal one third of the nucleus oralis (Vo) and in the rostralmost part of the Vo. Neurons projecting to the medial part of the posterior folia of crus II (crus IIp) were located in the dorsal to ventral parts of the Vi and of the caudal one third of the Vo and in the rostralmost part of the Vo, while those projecting to the lateral part of crus IIp were confined to the ventral part of the Vi and of the caudal one third of the Vo. Neurons of the Vp also projected to all of these cortical areas. They were relatively confined to the ventral part of this nucleus. Thest trigeminocerebellar projections were exclusively ipsilateral to the cell origin. There were sparse projections from the Vi and Vo to lobules V to VIIIa. In addition, a small group of neurons in the subnucleus magnocellularis of the nucleus caudalis of the Vsp also projected to the above cortical areas. No projections were, however, observed to the anterior portion of the anterior lobe, crus I, the anterior folia of crus II, paraflocculus, flocculus and the ventral part of the PMD. The majority of these cerebellar projection neurons were medium-sized and triangular, fusiform or ovoid in shape. There were small neurons of similar types and large multipolar neurons as well.


Primary afferent fibers in the tract of Lissauer in the rat.

More than two-thirds of the axons in the tract of Lissauer at mid-thoracic and lumbosacral levels of the rat spinal cord are primary afferent fibers. The proportions of primary afferents in the tract are approximately the same at the two spinal levels. A slightly higher percentage of the unmyelinated, as opposed to the myelinated, fibers are primary afferents. There is a somewhat greater percentage of primary afferent axons in medial parts of the mid-thoracic levels, but all areas of the tract that were examined contain a majority of primary afferent fibers. The primary afferent axons appear to travel less than a segment in the tract at mid-thoracic levels but for several segments in the tract at lumbo-sacral levels. These data indicate that the tract of Lissauer is predominately a primary afferent fiber system in these segments of the rat.


Commissural and intrinsic connections of the rat hippocampus.

The commissural and intrinsic connections of the hippocampus were studied using the Fink-Heimer method and the horseradish peroxidase (HRP) uptake technique. A conspicuous septo-termporal gradient was found of the density of the commissural projection that passes through the psalterium ventrale to the Ammon's horn. The degeneration resulting from transection of the psalterium ventrale was most dense in the septal tip and decreased towards the temporal tip. The commissural and ipsilateral connections from the hilus fasciae dentatae (CA4) and regio inferior (CA3/CA2) were found to terminate in different parts of the hippocampus. The hilus fasciae dentatae gave rise to ipsilateral and commissural projections to the dentate area only. The regio inferior has ipsilateral and commissural projections to the Ammon's horn. A specific termination pattern was found of the projection from regio inferior to stratum radiatum of both the ipsilateral and contralateral regio superior (CA1) and regio inferior (CA2/CA3). At levels temporal to the lesion, the projection is primarily to the superficial part of stratum radiatum, while at levels septal to the lesion the terminal zone occupies the deep part of the layer. This pattern was not related to the position of the cells of origin, along the septo-temporal or subiculo-dentate axes. In general, the commissural projections showed the same degree of septo-temporal divergence as the ipsilateral projections. The only major difference in the terminal fields of the two sets projections to the Ammon's horn was that the terminal zone of the commissural projection to stratum oriens was always more dense than that of the ipsilateral projection to this layer, while an inverse gradient was seen in stratum radiatum. The projections from the septal and middle dorso-ventral parts of regio inferior differed. The temporal spread of the projections from the septal part was large while that from the projections arising at middle dorso-ventral levels was more restricted. Moreover, a longitudinal association path interconnecting different parts of the regio inferior along the septo-temporal axis was seen to arise only from the cells in the septal parts of the regio inferior. Each part of the regio inferior projected to all parts of stratum radiatum and oriens of the contralateral Ammon's horn. However, the projection to the contralateral regio inferior was most dense at the site homotopic to that lesioned. The ventricular part of regio inferior projected primarily to the contralateral stratum oriens of the Ammon's horn, while the part adjacent to the dentate area mostly supplied stratum radiatum.


Electron microscopic studies of serially sectioned cat spinal alpha-motoneurons. II. A method for the description of architecture and synaptology of the cell body and proximal dendritic segments.

The paper presents a method for ultrastructural analysis and description of neuronal architecture and synaptology of cat spinal alpha-motoneurons from complete series of consecutive ultrathin sections through the cell body and proximal parts of the dendrites. The method implies that sections are selected for analysis only at certain constant intervals in the series. The occurrence of boutons of different morphological types on the neuronal surface was expressed by their percentage covering of the neuronal membrane. The neuronal surface was divided into a number of compartments and the synaptic covering was calculated separately for each compartment. An interval of 6 micrometer between the sections was used for these calculations, and the obtained values for synaptic covering were found not to differ significantly from those obtained in controls at 3 micrometer intervals. The number and location of individual large boutons (C- and M-types) were studied at 3 micrometer section intervals, and the escape of boutons connected to this procedure was estimated from control observations at 1 micrometer intervals. It is concluded that detailed information on neuronal synaptology can be obtained with this method, which will be used in three subsequent studies on functionally identified and intracellularly stained cat alpha-motoneurons.


Electron microscopic studies of serially sectioned cat spinal alpha-motoneurons. IV. Motoneurons innervating slow-twitch (type S) units of the soleus muscle.

Two intracellularly stained cat alpha-motoneurons of the soleus-S type (Burke et al., '74) were studied ultrastructurally. The architecture and synaptology of the cell body and proximal parts of the dendrites were analyzed from a long series of consecutive sections (Conradi et al., '79). Only few of the dendrites had a base diameter exceeding 10 micrometer. The proportion of the membrane surface of the cell body and dendrites covered by boutons was 40-45% and 50-80%, respectively. Out of this, 15-20% was constituted by S-boutons and 70-75% by F-boutons in both regions. In contrast to the situation in the gastrocnemius FR-neurons (Kellerth et al., '79) the large boutons of the C-type showed no clustering around the dendritic roots and were absent on the axon hillock. In addition, the M-boutons of dorsal root origin were more sparse on the proximal parts on the dendrites of the soleus-S neurons.


An autoradiographic study of the subcortical projections of the rabbit striate cortex in the adult and during postnatal development.

The subcortical projections of the rabbit striate cortex were studied by autoradiography. Small injections of 3H-leucine were placed into the striate cortex of 15 rabbit pups aged between one day and one month and six adult rabbits (strain Chbb:Ch). Terminal fields of projection occurred only ipsilateral to the injection, and these were found in the caudate nucleus (CN), the claustrum (Cla), the thalamic reticular nucleus (TRN), the dorsal (LGNd) and the ventral (LGNv) lateral geniculate nuclei, the pulvinar (Pul), the ventrolateral (Vl) the posterior (Po) thalamic nuclei, the anterior (APN) and posterior (PPN) pretectal nuclei, the nucleus of the optic tract (NOT), the superior colliculus (SC) and the basal pontine nuclei (PN). Cla was innervated via the superficial corticocortical and CN via the corticocaudate pathways. The remaining projections were innervated via the subcortical pathway which comprised three bundles: The deep bundle (to TRN, LGNd, Pul and Po), the superficial bundle (to LGNv, APN, PPN, NOT, and SC), and the peduncular bundle (to PN). The projection to SC showed remarkable changes of its terminal pattern during the first and second postnatal weeks. These changes were characterized by a strong reduction of the horizontal extension of the terminal labelling and an increasing concentration on the stratum griseum superficiale. Similar changes were not observed in the other projections.


The effect of hemidecortication on the inhibitory interactions in the superior colliculus of the cat.

Single neurons were recorded extracellularly from the superficial layers of the superior colliculus (SC) in 21 curarized cats. Four animals were normal unoperated cats, 17 were animals in which all cortical visual areas were ablated on one side from 7 to 69 days before the electrophysiological experiments. After cortical ablation all animals were blind in the visual field contralateral to the ablated side. In both normal and hemianopsic cats the effect of a visual stimulus located very far from the excitatory part of the unit receptive field, on the neuron responses to visual stimuli was studied. The remote stimulus (extra-field stimulus) was a hand moved black spot 10 degrees in diameter. In normal animals the introduction of the extra-field stimulus in the hemifield contralateral or ipsilateral to the recorded SC produced a marked reduction of unit responses to visual stimuli presented in their receptive field. This effect was particularly strong when the extra-field stimuli were introduced in the hemifield contralateral to the recorded side. In the hemianopsic animals the neurons of the SC ipsilateral to the lesion (receptive fields in the behaviorally blind hemifield) responded well to visual stimuli, but were only weakly inhibited by the extra-field stimuli presented in the blind hemifield. The neurons of this colliculus with the exception of those in the upper part of stratum griseum superficiale were normally inhibited by stimuli presented in the normal hemifield. The neurons of the SC contralateral to the lesion responded well to visual stimuli and were normally inhibited by stimuli presented in the normal hemifield; they were virtually not affected by stimuli presented in the blind hemifield. Mechanisms responsible for the abnormal inhibitory interactions between and within colliculi after cortical lesions and the possible behavioral implications of the findings are discussed.


Aging in the rat olfactory bulb: quantitative changes in mitral cell organelles and somato-dendritic synapses.

Quantitative measurements of the major organelles in the mitral cell perikaryon as well as numbers of mitral somato-dendritic synapses, have been made on electron micrographs from rats aged 3 to 30 months. The volume fraction of the cisternae of rough endoplasmic reticulum (RER) is constant throughout the period studied. Hence the amount of RER per cell reflects changes in perikaryal size. Thus there is a 3-fold increase of volume of cisternae of RER per mitral cell from 3 to 27 months and a halving from 27 to 30 months. A similar pattern is seen for the volume of mitochondria per cell. The volumes of dense bodies and cisternae of the Golgi complex per cell show a different pattern, that of a linear increase throughout the period, with no suggestion of a decrease from 27 to 30 months. An interesting finding is that the volume fraction of ground substance (perikaryal cytoplasm exclusive of the measured organelles) shows a remarkably constant value from 3 to 27 months and then a highly significant decrease from 27 to 30 months. This decrease, restricted to the period from 27 to 30 months, suggests a fundamental breakdown in cellular homeostasis in the oldest animals. Numbers of somato-dendritic, mitral-to-granule synaptic junctions per mitral cell and per olfactory bulb show a significant increase from 3 to 24 and 27 months, respectively, and then a suggestive decrease from 24 and 27 to 30 months. This finding indicates that new synapse formation is possible in normal adult rats, perhaps even in quite old rats.


Posttraumatic intracranial meningiomas: recognition by computed tomography in three cases.

Intracranial meningiomas were detected by computed tomography (CT) in three patients who had suffered previous head trauma 40, 10, and 3 years before. In two of the patients, the meningioma was located at the site of the traumatic impact and lesion. Meningiomas should be considered one of the late posttraumatic sequelae that can be recognized by CT.


Case report. The iron rim sign.

A chronic cystic cerebral hematoma occurred in the frontal lobe and mimicked both clinically and radiographically a frontal sinus mucocele. A rim seen on multidirectional tomography and unenhanced computed tomography was presumed to be composed to calcium. However, electron microscopic analysis revealed the rim to be composed almost entirely of iron from hemosiderin breakdown products. This is the first such report of an "iron rim" that we could find in the literature.


Case report. Diagnosis of Pick's disease by computed tomography.

Pick's disease is a rare cause of dementia. It often shows a characteristic pattern of atrophy affecting primarily the anterior portions of the frontal and temporal lobes. This pattern, as shown by computed tomography, may suggest the diagnosis.


Case report. Preoperative localization of a parathyroid adenoma using computed tomography and thyroid scanning.

We report a case of parathyroid adenoma correctly localized prior to operation by computed tomography (CT) and thyroid scanning. The use of CT and other noninvasive diagnostic modalities to aid the surgeon prior to initial operation for parathyroid adenoma is discussed.


Case report. Computer assisted tomographic evaluation of a ganglioneuroblastoma in an adult.

A case of ganglioneuroblastoma in an adult is presented. The precise localization of the tumor mass with computer assisted tomography aided the planning of radiation therapy. In addition, follow-up scans allowed evaluation of tumor response to both radiation and chemotherapy. The clinical and radiologic characteristics of this unusual tumor are discussed.


Case report. Renal oncocytoma.

In a case of renal oncocytic adenoma, a "spoke-wheel" pattern of pathological vessels was observed at arteriography of the highly vascular tumor. Computed tomograpy revealed a solid mass with attenuation value significantly higher than that of the normal renal tissue.


Technical note. Recognition of head motion during cranial computed tomography.

A television monitoring technique was developed to analyze patient head motion during cranial computed tomography (CT). The graphs recorded with this system, craniokymograms, are compared with simultaneous CT images. Two other applications of this system are shown with examples of experimental data.


Technical note. Optimal computed tomography technique for bone evaluation.

Various combinations of window settings and displays were evaluated to determine the optimal photographic reproduction of images obtained from the Ohio-Nuclear Delta scanner to best demonstrate bony structure by computed tomography. A combination of wide window and negative images will produce better bony structure detail than various other settings. The physical basis of this technique will be described along with several cases to illustrate the conclusion. Although no one combination was universally adequate, the best in our experience was a wide window with negative images.


Computed tomography of calcified liver metastases.

The computed tomography findings of six patients with calcified liver metastases are presented. The significance of this finding in patients with unknown primary malignancies or after multiple courses of chemotherapy is stressed.


An analysis of the spatial distribution of attenuation values in computed tomographic scans of liver and spleen.

An autocorrelation analysis of the attenuation values in computed tomographic scans has been performed for a total of 35 normal and eighteen cirrhotic patients. The results confirm preliminary observations on a smaller sample which suggested that this technique may be used to separate normal and cirrhotic livers. The noise structure in a region within the spleen was also examined and found to change in the presence of cirrhosis.


Computed tomography in the evaluation of abdominal trauma.

Four patients with blunt abdominal trauma underwent computed tomography (CT) as part of their diagnostic evaluation. In each case CT accurately demonstrated the pathology and the extent of injury. The results suggest that routine use of CT scanning may be an effective screening modality in the traumatized patient. It is perhaps more specific than radioisotope scanning and may eventually reduce the need for invasive techniques such as angiography.


Computed tomography of the prostate.

The conventional anatomy of the prostate is reviewed and the computed tomography (CT) anatomy described and illustrated. The results of 55 "normal" cases were analyzed for size and relationship to the symphysis pubis, retropubic space, and bladder, as shown on CT sections correlating the features with age and possible urinary symptoms. Attention is also drawn to the differences between phleboliths and prostatic calcification. Computed tomography is an effective method of demonstrating the prostate and surrounding structures and of assessing prostatic enlargement.


Computed tomography reconstruction from hollow projections: an application to in vivo evaluation of artificial hip joints.

The long-term mechanical stability of total hip arthroplasties limits the application of this widely used surgical procedure in cases of severe coxarthroses. A noninvasive method of assessing the tissue distribution in the vicinity of the implant--bone interface is needed to identify early signs of implant loosening and to evaluate new prosthetic solutions. In order to develop such a method, a modified computed tomography technique has been devised that yields cross-sectional images without the usual artifacts caused by metallic implants. This technique provides information on bone mineral and bone cement distributions as well as on the precise position and orientation of the implant within the femur shaft. Results obtained from excised human femurs and preliminary data from measurements on patients made 3 months postoperatively reveal that the bone cement distribution is much less favorable than is indicated by radiographs.


Computed tomography in bone and soft tissue pathology of the extremities.

Forty patients suffering from various pathological conditions of the extremities were examined by computed tomography (CT) and the results were correlated with conventional imaging techniques: plain radiography, conventional tomograms, angiograms, and radionuclide scanning. The patients' ages ranged from 4 to 80 years. The study included cases of osteomyelitis, benign and malignant bone and soft tissue tumors, metabolic disorders, and a case of pulmonary osteoarthropathy. We were able to follow progression and/or regression of osteomyelitis by recording the changes in the attenuation coefficient of the medullary cavity and also were able to determine the extent of the malignant infiltration of the marrow cavity. Contrast enhancement was valuable in demonstrating the vascularity of soft tissue tumors and in determining the extension of primary bone lesions.


Computed tomography in unilateral extremity swelling of unusual cause.

Computed tomography (CT) were used in the evaluation of unilateral soft tissue swelling of unusual cause. Three patients with unilateral myositis are described and the diagnostic information provided by the CT scan is discussed. A fourth patient with lymphedema praecox and unilateral extremity swelling is presented.


Two methods for calculating regional cerebral blood flow from emission computed tomography of inert gas concentrations.

Two methods are described for calculation of regional cerebral blood flow from completed tomographic data of radioactive inert gas distribution in a slice of brain tissue. It is assumed that the tomographic picture gives the average inert gas concentration in each pixel over data collection periods of 30 to 60 sec. In the early picture method a single picture taken during maximum inert gas concentration (by intaarterial injection or by inhalation) is analyzed. In the sequence of pictures method the alterations in local inert gas concentration are followed over time. The proposed methods are implemented using synthetic data of xenon-133 emission computed tomography and some of the difficulties likely to be encountered in practice are stressed.


Optimal processing of computed tomography images using experimentally measured noise properties.

The spatial resolution and noise properties of a computed tomography (CT) image may be altered by two-dimensional linear filtering of the initial image. In this paper, we derive filters that minimize the noise variance subject to a constraint on the spatial resolution. The resulting filter functions can reduce the noise variance by 17% in comparison with conventional filters. The method for obtaining these filters requires knowledge of the noise and imaging properties of the system. We derive theoretical expressions for these properties and introduce experimental techniques for their measurement. The statistical characteristics are shown to be anisotropic, spatially variant, and object dependent. We discuss the implications of this result both for optimal filtering and for the general problem of CT image noise property measurement.


Theoretical resolution of computed tomography systems.

A simple analytic model is presented which is used to calculate the impulse response of a computed tomography system for parametric variations of the geometry. The model is useful for fan beam as well as parallel beam geometries. Results show that wide-aperture detector systems have intrinsic resolution limitations that are not mitigated by oversampling. Furthermore, the optimum focal spot size of the X-ray source is found to be dependent on the detector geometry.


Lissencephaly (agyria) on computed tomography.

Unusual computed tomographic findings were observed in two infants with similar clinical pictures including delay in developmental milestones, seizures, decerebrated posture, and microcephaly. Computed tomography revealed small brain with widened subarachnoid space, smooth surface of the brain, uniformly enlarged ventricles, wide sylvian cisterns, and lack of insular opercularization. These findings coincide with pathologic features of lissencephaly (agyria), a rare congenital anomaly of the brain due to interruption of neuronal migration.


Multiple cystic encephalomalacia of infancy: computed tomographic findings in two cases with associated intracerebral calcification.

Two initially healthy infants developed acute encephalopathic illnesses characterized by stupor, seizures, cerebrospinal fluid (CSF) erythrocytic and monocytic pleocytosis, increased CSF protein, and decreased CSF glucose and progression to chronic decerebration. In one case, herpes simplex virus was recovered from cutaneous lesions. The initial computed tomography (CT) scan revealed widespread subcortical increased attenuation with further increase after contrast medium injection and patchy areas of decreased attenuation in the deep cerebral white matter. Subsequent CT scans demonstrated progressive cortical calcifications and persistence of low attenuation areas. Autopsy revealed multiple cystic encephalomalacia. The second infant had similar clinical, CSF, and CT findings but remains in a chronic decerebrate state at 14 months of age. The CT abnormalities seen in these patients have not been encountered in any of 13 other infants with the clinical diagnosis of meningitis or encephalitis. Multiple cystic encephalomalacia of infancy is a  rare condition with a uniformly bleak prognosis. Computed tomography may prove useful in the early diagnosis, thereby aiding clinicians in counseling and in the acute and long-term management of patients with this lesion.


Exploratory laboratory studies of the relative aversiveness of traffic sounds.

Two experiments examined the acoustical correlates of traffic sound aversiveness. In experimnet I all pairs of 13 45-s traffic sound recordings were presented; subjects chose the sound of each pair to which they would rather be exposed and judged the relative similarity of the sounds. The energy equivalent sound level, Leq, accounted for choice and similarity judgments better than any other noise index, and there was no substantial improvement in ability to account for subjective judgments when other indices were used in conjunction with Leq. In experiment II subjects judged the same sounds which had been made equal in Leq level. Responses were nonrandom, showing that Leq is not the only subjectively important information in traffic sounds, but no acoustical measure employed in the study captured that information. Instead, two subjective measures of information content accounted best for the data of experiment II. Response data for the equal Leq sounds were less structured than for the sounds differing in Leq. The data of the two experiments were statistically related; it is argued that the data sets were related via the measure of information content. Experiment I replicated a previously reported study, while experiment II failed to replicate the results of a second study.


Development of noise and vibration ride comfort criteria.

A laboratory investigation was directed at the development of criteria for the prediction of ride quality in a noise-vibration environment. The stimuli for the study consisted of octave bands of noise centered at 500 and 2000 Hz and vertical floor vibrations composed of either 5 Hz sinusoidal vibration, or random vibrations centered at 5 Hz and with a 5 Hz bandwidth. The noise stimuli were presented at A-weighted sound pressure levels ranging from ambient to 95 dB and the vibration and acceleration levels ranging from 0.02--0.13 grms. Results indicated that the total subjective discomfort response could be divided into two subjective components. One component consisted of subjective discomfort to vibration and was found to be a linear function of vibration acceleration level. The other component consisted of discomfort due to noise which varied logarithmically with noise level (power relationship). However, the magnitude of the noise discomfort component was dependent upon the level of vibration present in the combined environment. Based on the experimental results, a model of subjective discomfort that accounted for the interdependence of noise and vibration was developed. The model was then used to develop a set of criteria (constant discomfort) curves that illustrate the basic design tradeoffs available between noise and vibration.


Effects of stimulus frequency on adaptation in auditory-nerve fibers.

Several experimental methods of depressing the response of auditory-nerve fibers to tonal stimuli have been shown to reduce the response to signal frequencies at or near fiber CF (characteristic frequency) more than to frequencies greater or less than CF. In this study we have used a short adapting tone presented before each test-tone burst to reduce the fiber's response to the test tone. We observed the effects of changing test frequency when the adapting frequency was held constant at fiber CF. The depression in discharge rate was found to be approximately constant across test frequency.


Adaptation, saturation, and physiological masking in single auditory-nerve fibers.

Results are reviewed concerning some effects, at a units's characteristic frequency, of a short-term conditioning stimulus on the responses to perstimulatory and poststimulatory test tones. A phenomenological equation is developed from the poststimulatory results and shown to be consistent with the perstimulatory results. According to the results and equation, the response to a test tone equals the unconditioned or unadapted response minus the decrement produced by adaptation to the conditioning tone. Furthermore, the decrement is proportional to the driven response to the conditioning tone and does not depend on sound intensity per se. The equation has a simple interpretation in terms of two processes in cascade--a static saturating nonlinearity followed by additive adaptation. Results are presented to show that this functional model is sufficient to account for the "physiological masking" produced by wide-band backgrounds. According to this interpretation, a sufficiently intense background produces saturation. Consequently, a superimposed test tone cause no change in response. In addition, when the onset of the background precedes the onset of the test tone, the total firing rate is reduced by adaptation. Evidence is reviewed concerning the possible correspondence between the variables in the model and intracellular events in the auditory periphery.


Sexual characteristics of preadolescent childrens' voices.

This investigation was undertaken to enlarge current understanding of the acoustic properties which influence the perception of maleness and femaleness in the voices of prepubertal children. Perceptual judgments of sexual identity were obtained in response to tape recordings of whispered and normally phonated vowels, normally spoken sentences, and sentences spoken in a monotonous fashion. Seventy-three children provided recordings. The four utterance types were chosen to experimentally manipulate selected physical properties of speech thought to exert an influence on listener judgments of sexual identity. The results of this work suggest that cues stemming from differences in vocal tract dimensions and/or articulatory behaviors provided the primary cues about the sexual identity of these preadolescent children. Although laryngeal source cues could have provided relevant information about the sex of a few children, this variable was felt to play a relatively minor role in the sex recognition process. New information was uncovered about the role certain suprasegmental factors play in the identification of child sex.


Perceptual origins of the phoneme boundary effect and selective adaptation to speech: a signal detection theory analysis.

Differences in discriminability of stimuli near phoneme boundaries and findings from selective adaptation have been used to argue for the existence of neurophysiological mechanisms--feature detectors--which mediate the perception of speech and speechlike sounds. A detection theory model was used in order to discover whether or not the phoneme boundary effect and the shift in phoneme boundary after adaptation might rather be attributable to changes in response bias. This model was applied in the analysis of phoneme identifications of three sets of stimuli before and after adaptation. While the origins for the phoneme boundary effect appear to lie below the level of response bias, findings suggest that identification changes after adaptation may be due solely to shifts in criterion, rather than changes at the sensory level.


Developmental study of vowel formant frequencies in an imitation task.

Imitations of ten synthesized vowels were recorded from 33 speakers including men, women, and children. The first three formant frequencies of the imitations were estimated from spectrograms and considered with respect to developmental patterns in vowel formant structure, uniform scale factors for vowel normalization, and formant variability. Strong linear effects were observed in the group data for imitations of most of the English vowels studied, and straight lines passing through the origin provided a satisfactory fit to linear F1--F2 plots of the English vowel data. Logarithmic transformations of the formant frequencies helped substantially to equalize the dispersion of the group data for different vowels, but formant scale factors were observed to vary somewhat with both formant number and vowel identity. Variability of formant frequency was least for F1 (s.d. of 60 Hz or less for English vowels of adult males) and about equal for F2 and F3 (s.d. of 100 Hz or less for English vowels of adult males).


Cochlear action potential threshold and single unit thresholds.

There is a close correlation between the sound pressure of tone burst required to affect a primary auditory neuron at its characteristic frequency and that which will produce a detectable N1 response at the same frequency. Units with thresholds from 80--0 db SPL (recorded from damaged and undamaged cochleas) were 0--20 dB , respectively, more sensitive than the action potential response.


Influence of earphone-cushion center-hole diameter on the acoustic output of audiometric earphones.

Although the dimensions of the MX-41/AR earphone cushion are specified in ANSI S3.6--1969, nonstandard MX--41/AR cushions are still found on some audiometers. Variations in the diameter of the cushion's center hole are shown to affect the coupler calibration of the earphone and also hearing threshold measurements at high audiometric frequencies.


The effect of bone conduction on the intensity independence of dichotic chords.

The relative salience of the pitch components of a two-tone dichotic chord is invariant with respect to the relative intensity of the two tones over a wide range of interaural intensity differences [R. Efron and E. W. Yund, J. Acoust, Soc. Am. 889--898 (1976)]. According to a recently developed model, the range of intensity independence is limited by the bone-conducted energy from the more intense tone [E. W. Yund and R. Efron, J. Acoust. Soc. Am. 62, 607--617 (1977)]. The model predicts that a decrease in bone conduction such as the one achieved by using insertion earphones, must increase the range of intensity independence. This prediction is confirmed.


Control of vasospasm following trauma and microvascular surgery.

Vasospasm may result in marked reduction of blood flow through injured or revascularized tissues. Neurogenic vasoconstriction is mediated through sympathetic pathways which may be blocked by long-acting local anesthetics resulting in optimal intravascular pressures and elevated flow. An experimental study performed on normal human volunteers has shown that distal sympathetic blockade using Bupivacaine protects the digital circulation against vasospasm elicited by a strong stimulus for a minimum of 7 to 8 hours. A catheter, placed adjacent to the median or ulnar nerve at the wrist level after digital microvascular reconstruction, facilitates continuous Bupivacaine sympathetic blockade. This technique has been used clinically and appears to improve the circulation of replanted or revascularized tissues and protects against vasospasm caused by a variety of stimuli.


Prevention of femoral vein occlusion by local injection of Thrombolysin in the rat.

An experimental model was developed that will produce femoral vein occlusion greater than 90% of the time in rats weighing less than 500 grams. This standard venous clot, produced by combining intimal trauma with local venous stasis, can simulate clinical crush and replantation problems. Using this standard clot model, Thrombolysin was injected in the femoral artery after trauma to the vein but before venous stasis. Femoral vein patency was determined at the end of the 30 minute period of venous stasis. With the appropriate dosage, ipsilateral venous occlusion was prevented (P less than 0.00001) without preventing contralateral venous occlusion (P less than 0.02). Except for decreased fibrinogen levels, there was no significant change in the factors studied between the control and experimental groups. It is possible, therefore, to prevent thrombosis in a vein with intimal damage, even in the presence of a low-flow state by the local injection of Thrombolysin, without producing systemic anticoagulation.


A complex reimplantation of digits: a case report.

In multiple finger amputations, microsurgical reconstruction should concentrate on the thumb, index, and middle fingers. The patient in the case report presented in this paper initially sustained an amputation of all of the digits on the left hand and an amputation of the right thumb. Both thumbs were amputated at the metacarpophalangeal joint. Initial replantation of the left thumb, index, and middle fingers and of the right thumb was carried out. The right thumb replantation failed and, after healing, the right great toe was transferred to the right hand. Subsequent to this, the patient developed useful function of all reconstructed digits. Twenty-one months after the first injury, both thumbs sustained new traumatic amputations 1.0 cm distal to the previous amputations. Both thumbs were replanted again successfully.


Lipofibroma of the median nerve.

Tumors arising within the median nerve in the region of the distal forearm, wrist, and palm are rare and their exact pathological nature has not been well clarified. One case is reported in a 47-year-old woman, in whom a mass of 3 years' duration was located in the thenar eminence of the right hand without causing any sensory or motor deficit. The tumor was surgically excised, and the continuity of the nerve branches was preserved. The diagnosis of lipofibroma of the median nerve is recommended for this entity. Other pathological lesions of the median nerve are mentioned.


Prevention of extensor lag after indicis proprius tendon transfer.

An experiment on fresh cadaver hands showed that a longitudinal incision of the hood did not affect index extension when traction was made on the common extensor tendon, but that excision of a portion of the hood containing the indicis proprius caused an extensor lag. The lag was eliminated by repair of the hood, providing that the closure was not too tight to prevent normal excursion of the hood. These findings were correlated with findings in patients who had undergone indicis proprius tendon transfer. Extensor lag after indicis proprius transfer is not caused by removal of the force of the tendon per se, but by factors which cause either disruption of normal hood function or tethering of its normal excursion.


Locking of the metacarpophalangeal joint: a case report.

A case of an unusual cause of locking of the finger is described. This was brought about by the presence of an exostosis arising from the metacarpal bone, causing rupture of the capsule of the metacarpophalangeal joint with entrapment of the interosseus tendon.


Entrapment neuropathy due to bilateral epitrochleoanconeus muscles: a case report.

A case of bilateral entrapment of the ulnar nerves due to accessory epitrochleoanconeus muscles was observed in a young athlete. Hypertrophy of this muscle may be one of the causes of cubital tunnel syndrome.


Another cause of median nerve compression by a lumbrical muscle in the carpal tunnel.

A case is described in which division of a flexor digitorum profundus tendon allowed the proximal end of the tendon to retract, drawing its lumbrical muscle into the carpal tunnel where it caused compression of the median nerve.


Life changes and menstrual discomfort.

The present study addressed the relationship between the accumulation of life changes and problems with menstruation. Female college students completed a Life Experiences Survey, which assesses desirable and undesirable life changes, and a menstruation questionnaire, which contains items relevant to menstrual discomfort and irregularity. Correlational analyses showed that undersirable life changes were correlated with reports of number of symptoms of menstrual discomfort, but not irregularity. Separate analyses among oral contraceptive users and nonusers showed that the relationship between life changes and menstrual discomfort occurred only among nonusers. A regression of contraceptive use, desirable life changes, and undersirable life changes on factors derived from the menstruation questionnaire indicated that undesirable life changes was the strongest predictor of menstrual problems.


Nutrition and breast cancer.

Present evidence suggests that a high fat intake may be one of the factors in the aetiology of breast cancer. Patients with breast cancer may show an increased requirement for thiamin particularly when treated with 5-fluorouracil, and a number of metabolic disturbances in which ascorbic acid may play a central role.