Periodontal disease and oral hygiene in a group of blind and sighted Israeli teenagers (14--17 years of age.

The purpose of the present study was to obtain up-to-date information on periodontal disease and oral hygiene status in Israel among 9th to 12th grade non-blind students and among the inmates of six schools for the blind aged 14--17 years. 460 sighted and 434 blind students participated in the study. The examination was for periodontal status and oral hygiene only. Periodontal status was recorded according to Russell's Periodontal Index (PI). Oral hygiene was assessed according to Greene & Vermillion's Simplified Oral Hygiene Index (OHI-S). Mean PI for the non-blind group was found to be 0.23, and for the blind students 0.79. Partially blind students were found to have a lower mean PI value than the totally blind. PI values did not increase significantly with age. Blind students exhibited a fair-to-poor level of oral hygiene (according to Greene & Vermillion's categorization). Relatively healthier levels of oral hygiene were found in the partially blind as compared with the totally blind. Mean OHI-S did not vary with age in either group.


Frequency and distribution of myofascial pain-dysfunction syndrome in a population of 25-year-olds.

Interviews on the occurrence of Myofascial Pain-Dysfunction (MPD) Syndrome were held with a group consisting of 246 individuals 25 years of age. Every fifth interviewee said she/he had experienced clicking/crepitation from the TMJs. Five percent had felt pain when opening the mouth wide and 3% while chewing, yawning or talking. These frequencies of symptoms were much like those found in a probability sample of the total adult population in Norway and a probability sample of elderly Norweigians. In the present study, 28% said they had some or much headache recently. These symptoms varied with background characteristics, especially with sex. There was also a relationship between headache and MPD-symptoms. By combining the effect of the variables sex and headache upon the frequency of reported clicking/crepitation, it was tripled.


Intermediate dose methotrexate (IDM) in childhood acute lymphocytic leukemia (ALL).

We employed three courses of intermediate dose Methotrexate (IDM) added onto a standard induction and maintenance program with the concept of both central nervous system (CNS) prophylaxis and simultaneous systemic intensification. Cranial radiation (RT) was not employed as CNS prophylaxis. Fifty of 52 patients (to age 18) achieved complete remission. Time on study now ranges from 22-68 months with a median time of 33 months. We separated the children into standard risk and increased risk. We defined increased risk as a WBC over 30 000/mm3 at presentation and an age of less than two years or greater than 10 years at presentation. There have been 15 relapses on these 50 patients; 11 occurred in increased risk patients (of 22 increased risk patients) and four occurred in standard risk patients (of 28 standard risk patients). There were seven CNS relapses, six systemic relapses, one simultaneous systemic and CNS relapse and one testicular relapse. Toxicity to the IDM was small with the worst problem being mucositis. No leukoencephalopathy occurred. The control of hematological relapse is excellent and the avoidance of potential long-term complications notes is even of greater importance.


Alterations in translational control mechanisms in Friend erythroleukemic cells during DMSO induced differentiation.

Induction of erythroid differentiation in Friend erythroleukemia cells causes a reduction in the rate of protein synthesis and a prolonged growth cycle of the cells. A study of the relative translational efficiencies (RTE) of viral mRNAs and selected host cell mRNAs revealed unexpected low RTEs of the mRNA coding for the gp70 protein and RTE of the mRNA coding for the p30 protein. The RTEs of viral and of some host mRNAs show significant changes during differentiation. The rate of synthesis of corresponding proteins are altered non-coordinately, indicating transcriptional and/or posttranscriptional regulation of mRNA, as well as amplified regulation at the level of translation of mRNA.


Histopathology of bone marrow in human chronic leukemias.

Among the patients with chronic myeloproliferative diseases including clinical symptoms of chronic myelogenous leukemia--CML--two varying compartments with substantially differing histology of hemopoiesis were found: one with predominating granulopoiesis for which the usual term of chronic granylocytic leukemia--CGL--seems inadequate. The other with proliferation of granylopoiesis and megakaryopoiesis as a neoplasia with a mixed cellularity is observed to be different in its clinical course: there are often a leukemic or subleukemic cell counts, but mostly considerable increased platelets in the peripheral blood; there is a prolonged period of latency, a higher age group, an infrequent occurrence of blastic crisis and a regular outcome into myelofibrosis. This entity of chronic megakaryocytic granulocytic myelosis--CMGM--can be seen very frequently among myeloproliferative diseases. Among a total of 718 core biopsies from the bone marrow the CMGM-patients are up to 29% compared with 21% of the typical one-cell-line disease CGL. The Ph1-chromosome may be presented in the CMGM-entity likewise.


Chemicals and industrial processes associated with cancer in humans. IARC Monographs, Volumes 1 to 20.

An international ad hoc Working Group of experts in cancer research met at the International Agency for Research on Cancer (IARC) in January 1979 to evaluate the data on human and experimental animal carcinogenicity for 54 chemicals, groups of chemicals, and industrial processes. Monographs for these chemicals were published in Volumes 1-20 of the IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. On the basis of evidence from human studies, 18 of the 54 chemicals and industrial processes are human carcinogens. A further 18 chemicals are probably carcinogenic for humans, although the data were considered not adequate to establish a causal association. To reflect differing degrees of evidence of carcinogenicity within this group, it was further subdivided; for six chemicals there was a high degree of evidence, and for 12 there was a lower degree. Data on the remaining 18 chemicals were considered insufficient to allow any evaluation of carcinogenicity. The report summarizes the background, purpose, and overall conclusions of the Working Group. The evidence supporting the evaluations is given in the Appendix. This volume includes a cumulative index of chemicals for Volumes 1-20 of the IARC Monographs, as well as an index by possible target organ in humans. A condensed version of this report will appear in the December 1979 issue of Cancer Research.


Sister chromatid exchange in Ph1-positive chronic myelocytic leukemia.

The sister chromatid exchange (SCE) frequency in bone-marrow cells of 12 untreated patients with chronic myelocytic leukemia (CML) was analysed and compared with the SCE frequency in bone-marrow cells of nine healthy persons. In normal persons the SCE ranged from 3.64 to 5.15 per cell. In CML patients the SCE was significantly lower, ranging from 2.32 to 3.44 per cell. The differences found were unrelated to patients' age and contraction state of the chromosomes. It is suggested that the leukemic process could account for the low SCE rate.


14q translocations, having a break point at 14q13, in lymphoid malignancy.

In three patients with non-Hodgkin lymphomas and in one cell line (HPL-Hod) derived from pleural effusion cells of a patient with Hodgkin's disease, rearrangements of the long arm of chromosome No. 14(14q) were observed. These rearrangements appeared to be consistently associated with a 14q translocation, suggestive of occurrence of a break at 14q13. The translocation in an individual case could occur with 1p, 2q, 4q, and another 14q. A 14q13 translocation may be comparable with a 14q32 translocation, which has often been observed in various types of lymphoid malignancy.


[Bone marrow lymphoid follicles in myelofibrosis compared with chronic myelogenous leukaemia (author's transl)].

The trephine bone marrow biopsies of 51 patients with myeloproliferative syndromes were revised searching for lymphoid follicles and lymphoplasmocytosis: 18 of these had idiopathic myelofibrosis and 33 chronic myelogenous leukaemia. Six of the 18 biopsies on patients with myelofibrosis showed lymphoid follicles but only one of the 33 with chronic meylogenous leukaemia did (P = 0.01, Fisher exact test). In addition, four of the six myelofibrosis having follicles had two or more of them. When the pathological pattern of myelofibrosis was considered according to the Lennert and al. classification we found significantly more follicles in the cellular phase of the disease than in the advanced phases (P = 00.4, Fisher exact test). These findings can be considered as a morphological argument supporting the idea of an immunological mechanism in the development of myelofibrosis.


The evaluation of tiodonium chloride as an antiplaque and anticaries agent. I. Preliminary in vitro and in vivo studies.

Tiodonium chloride was evaluated for its efficacy against Streptococcus mutans. Depending upon the concentration used, it was found to have either bacteriocidal or bacteriostatic activity against S mutans and ti inhibit the accumulation of plaque formed in vitro by this organism. When applied as a mouthrinse in hamsters infected with S mutans, tiodonium chloride significantly reduced the accumulation of dental plaque. Chlorhexidine gluconate was tested as a positive control in the in vivo experiment.


The evaluation of tiodonium chloride as an antiplaque and anticaries agent. II. In vitro antimicrobial activity and in vivo anticaries activity in animals.

The efficacy of tiodonium chloride as an antimicrobial, antiplaque, and anticaries agent was examined. It proved to be an effective antibacterial agent and reduced in vitro plaque formation at concentrations below its bacteriostatic level. It reduced caries in rats when used in the food at 600 microgram/gm but was not effective as a mouthrinse at up to 0.3%.


The evaluation of tiodonium chloride as an antiplaque and anticaries agent. III. Evaluation of the antiplaque potential of tiodonium chloride utilizing a rat model.

Tiodonium chloride (4-chlorophenyl-2-thienyliodonium chloride), when used in a twice daily mouthrinse at a concentration of 0.3% for either one or four weeks, inhibited dental plaque formation in rats that had been inoculated with Streptococcus mutans 6715-15 and Actinomyces viscosus T-6. Mouthrinses containing 0.1 and 0.2% tiodonium chloride were also effective in inhibiting plaque, but not as consistently as the 0.3% level.


The evaluation of tiodonium chloride as an antiplaque and anticaries agent. IV. Effectiveness on plaque and gingivitis in a short-term clinical study.

The purpose of this clinical investigation was to evaluate the effectiveness of 0.3% tiodonium chloride mouthrinse on various plaque and gingivitis indices. Fifty-five subjects were randomly assigned to the tiodonium chloride or placebo mouthrinses. After a prophylaxis on day 1 of the study, the subjects suspended all oral hygiene except for the daily supervised mouthrinsing. Plaque was monitored by thickness, area, and dry weight. All mean plaque scores were significantly lower in the tiodonium chloride group than in the placebo group (P less than 0.01). Gingivitis was assessed by a clinical gingivitis index and by measuring the amount of crevicular fluid. Mean clinical gingivitis scores (GI) did not differ significantly between the two groups; however the mean gingival fluid score was significantly lower in the tiodonium chloride group than in the placebo group (P less than 0.05). No side effects were reported or observed during the study.


Surface ultrastructure of some oral bacteria.

Adhesion of Streptococcus sanguis, Fusobacterium nucleatum and an Actinomyces sp. to enamel and epon and their interspecies cohesion was studied with scanning and transmission electron microscopy. For adhesion studies enamel or epon was coated with salivary macromolecules and then cells of S. sanguis and in some experiments also with F. nucleatum or Actinomyces sp. Cells of S. sanguis were seen scattered over the surface of a thin "pellicle" that was heavily stained, and F. nucleatum and Actinomyces sp. adhered to S. sanguis or directly to the "pellicle". For studies of cohesion S. sanguis was brought to cohere with F. nucleatum or Actinomyces sp. and then processed for transmission electron microscopy. The morphology of the cell surface structures involved was studied in negatively stained preparations or in thin sections of material treated with ruthenium red or poststained with uranyl and lead salts, phosphotungstic acid or periodic acid-thiocarbohydrazide-osmium tetroxide. S. sanguis demonstrated a fuzzy coat of fimbriae that seemed to unfold in areas of contact with other cells, while cells of F. nucleatum had 6-10 polar pilus-like fimbriae, which appeared to be instrumental in cohesion, as did a dense coat of long, slender fimbriae that covered cells of Actinomyces sp.


Access to interproximal tooth surfaces by different bristle designs and stiffnesses of toothbrushes.

An in vitro model for the purpose of testing the access to interproximal tooth surfaces by toothbrushes is described. The model was used to compare the access of different bristle designs and stiffnesses. Two standardized brushing techniques were used: a horizontal and a vertical one. The test method discriminated significantly between different bristle designs and stiffnesses. With regard to interproximal access the V-shaped bristles were superior to the straight multitufted ones and soft bristles were better than medium and stiff ones. This study also indicated that the interproximal access increased with increasing pressure within the pressure range chosen. Of the two techniques used in this study the vertical one was superior to the horizontal. The results indicated that some current opinions with regard to mechanical cleaning should be re-evaluated and clinical testing methods be further developed to enable corresponding in vivo testing.


Aberrant blastogenic response to LPS in experimental gingivitis of elderly subjects.

The blastogenic response of peripheral blood leukocytes to lipopolysaccharide (LPS), phytohemagglutinin (PHA) and LPS/PHA mixtures was followed over a short course of experimental gingivitis in elderly subjects (65-81 years) who strictly avoided oral hygiene procedures for periods up to 9 d. The leukocytes responded poorly to LPS, PHA and to LPS/PHA combinations. The concomitant heightened sensitivity of the gingiva to dental plaque among the elderly subjects may relate to the altered leukocyte response in this age group.


Location and variation in number of taste buds in human fungiform papillae.

Serial sections of 182 fungiform papillae, obtained at autopsy from 22 individuals aged 2 days to 90 years, were examined by light microscopy with regard to location and number of taste buds. The taste buds were always found on the convex, dorsal surface of the papillae but otherwise failed to display any preferential location pattern. A total of 262 taste buds, an average of 1.4 per papilla, were found. However, there was considerable variation in the occurrence of taste buds, both from papilla to papilla and from case to case. While the number of taste buds in a single papilla varied from 0 to 27, 63% of the papillae had no taste buds at all, 26% had 1-3 buds and the remainder 4 or more buds. The mean number of taste buds per papilla varied from 0 to 9 between individuals; no dependence upon sex or age could be demonstrated for this variation. The significance of these anatomical findings with regard to physiological studies on taste involving the fungiform papillae is discussed.


Autoradiographic study of distribution patterns of metals which occur as corrosion products from dental restorations.

Radioactive chromium, cobalt, gold and mercury, all of which may occur as corrosion products from dental restorations, were injected in single doses via the tail vein of mice and an in vivo analysis of their distribution was performed using whole-body autoradiography. Gold seemed to be inert and went mainly to the liver and bones. The other elements studied were distributed widely in the body and remained for a long period of time in specific target organs and tissues.


Levels of mercury and silver in dust from the trimming of amalgam dies.

The levels of mercury and silver in dust arising from the trimming, i.e. grinding, of amalgam dies in dental laboratories have been measured. In breathing air close to the workpiece, the mercury and silver contents exceeded the threshold limit values for short-term exposure by factors of aboit 60 and 400 in cases when local ventilation was not in use. With efficient local exhaust systems enabling a dust reduction of about 94%, the short-term exposure limit values for mercury and silver were exceeded by factors of about 4 and 20 respectively. Mercury and silver were assayed quantitatively by means of nuclear chemical analysis. A major part of the amalgam dust consisted of respirable particles. The collected dust comprised about 80% amalgam and 20% particulate matter from grinding wheels and stones according to SEM and EDAX measurements.


Toxic elements in silicate cements.

Six brands of silicate cements have been characterized by means of optical emission spectrography with respect to the contents of elements in minor or trace quantities in a search for presence of possible toxic elements. Beryllium was observed in two powders at levels of 1.3 and 1.6% Cadmium was found in two powders at levels of 0.02 and 0.03%. Lead was measured in three powders at levels of 0.001-0.003%. Bismuth, boron, copper, gallium, iron, manganese, titanium, tin and zirconium were found in various brands in either powder or liquid at levels of 0.001-0.1%. Upper limits of the amounts of the various elements that might be transferred to the gastrointestinal tract after dissolution of the cement matrix in the oral cavity have been calculated.


Mouse teratocarcinoma mutant clones deficient in adenine phosphoribosyltransferase and developmentally pluripotent.

Mouse teratocarcinoma stem cells deficient in activity of adenine phosphoribosyltransferase (APRT; EC 2.4.2.7) were obtained in order to have this marker in developmentally versatile cells. Mutagenized stem-cell cultures were selected for resistance to 8-azaadenine and four clonal cell lines were isolated. Three had severe deficiencies of APRT activity (7% or less of wild type) and one had a moderate reduction (73%). The enzyme in the latter clone was found to be an electrophoretic variant with slightly less anodal migration than the wild-type enzyme. Each clone remained stably APRT-deficient for at least 3 1/2 weeks, after subcutaneous inoculation, in the absence of the selective agent. The tumors formed from the inocula comprised a variety of differentiated tissues and thus showed persistence of stem-cell developmental pluripotency despite mutagenesis and selection. All mutants also retained the quasinormal karyotype (X/O sex chromosomal constitution, trisomy-19) of the parent line. These lines are appropriate for such uses as production (by blastocyst injection) of mouse models of the human genetic deficiency and for foreign-gene transfer, via teratocarcinoma cells, into mice.


Cytoplasmic genetics of mammalian cells: conditional sensitivity to mitochondrial inhibitors and isolation of new mutant phenotypes.

We report here that glucose, as a carbon source, and pyruvate are required for the phenotypic expression of cytoplasmically transmitted chloramphenicol-resistance (CAP-R) mutations, recovery of CAP-R mutants, and continuous growth in the presence of oligomycin or antimycin. We assume that glucose supplies additional energy when mitochondrial respiration is diminished and that pyruvate provides intermediates when the Krebs cycle is inhibited. Thus, the requirement for pyruvate is fully satisfied by an exogenous source of purines, and partially by alpha-ketoglutarate or a pyrimidine source. Based upon these findings, we have obtained two types of mutations affecting mitochondrial function--oligomycin resistance and pyruvate-independent expression of chloramphenicol resistance. Both are cytoplasmically transmitted and provide new markers for a genetic analysis of mitochondrial biogenesis.


Some thoughts about genetics, differentiation, and malignancy.

This article deals with three related questions: (1) whether malignancy is determined by genetic or epigenetic mechanisms; (2) whether epigenetic mechanisms, as conventionally defined, actually exist; (3) what criteria are appropriate for defining dominance or recessiveness of the malignant state in cell fusion experiments.


Activation of normal genes in malignant cells: activation of chemotaxis in relation to other stages of normal differentiation in myeloid leukemia.

Genetically differerent clones of myeloid leukemic cells have been used to study the activation of normal genes in these malignant cells by the normal physiological inducer of myeloid cell differentiation, the protein MGI. In appropriate clones, MGI induced the normal differentiation-associated property of chemotaxis to a variety of compounds including the steroid hormone dexamethasone. The induced cells could also distinguish among different steroids by chemotaxis, suggesting that there are specific membrane interaction sites for steroids. The sequence of differentiation in these cells was the formation of C3 and Fc rosettes leads to phagocytosis of these rosettes and chemotaxis leads to synthesis and secretion of lysozyme leads to mature macrophages or granulocytes. The use of appropriate mutants and the comparison of induction by MGI and dexamethasone has shown that chemotaxis to casein can be dissociated from: chemotaxis to dexamethasone, ATP, and bacterial factor; formation of C3 or Fc rosettes; phagocytosis of these rosettes; synthesis of lysozyme; and the formation of mature cells. It is suggested from this dissection of normal differentiation that there are different membrane changes for specific chemotaxis, formation of these rosettes, and their phagocytosis, and that induction of each of these properties requires activation of different genes.


Is Weinberg's differential rule valid?

Weinberg's differential method, whereby the frequency of dizygotic twins in a population is obtained by doubling the number of opposite-sexed pairs, might not be valid. Data are presented indicating a higher frequency of same-sexed than opposite-sexed pairs among samples of dizygotic twins ascertained at birth, thus favoring the hypothesis that, contrary to the method's fundamental assumption, the sexes of the two twin zygotes be not independent.


The first five years of the Budapest twin register, 1970-1974.

The Budapest Twin Register recorded 88.6% of the 1739 multiple births that occurred between 1970 and 1974. Of these, 31.2% were male-female pairs. Based on the examination of the placenta (19.8% monochorial) and of genetic makrers, 406 pairs proved to be monozygotic and 645 dizygotic. Sex ratio of twins at birth showed a female preponderance (1000 F: 1009 M) in comparison to singletons (1000 F: 1071 M). Average birthweight of the twins was 2101 g. The frequency of stillbirths was about four times higher in twins than in singletons, twice as high in monozygotic than in dizygotic twins, and 2.4 times higher among second than first-born twins.


Artificial ventilation in children during anaesthesia using a tidal volume ventilator.

Standards for artificial pulmonary ventilation at ventilatory frequencies of 15 and 20 cycles per min, (PETCO2 4 +/- 0.5 kPa), were defined in 16 children with healthy lungs and body weights between 2.6 and 22.6 kg. A tidal volume ventilator and balanced anaesthesia were used during abdominal surgery. At f = 20 cycles/min an approximately direct proportionality existed between tidal volume and body weight (VTpat = 12.3 . kg b.w.--2.1, r = 0.99). The mean value VTpat per kg b.w. was 12.1 +/- 1.4 ml/kg. At f = 15 cycles/min, a linear relationship between tidal volume and kg b.w. was recorded (VTpat = 14.6 . kg b.w. + 16, r = 0.97). The mean value VTpat per kg b.w. was 15.9 +/- 1.5 ml/kg. A significant decrease in total compliance was recorded during the initial part of the surgical procedure. A rectilinear relationship existed between total compliance and kg b.w. (CTOT = 11.2 . kg b.w.--12.5, r = 0.95). The mean value CTOT per kg b.w. was 9.7 +/- 0.54 ml/kPa/kg at f = 20. The endotracheal peak pressure maintained a fairly constant pressure level independent of the initial pressure within the ventilator, the tidal volume and the body weight, a phenomenon explained by the hypothesis that the proportional increase in total lung volume and functional residual capacity in the older children compensated for the higher pressures.


Acute myeloblastic leukemia following treatment for non-hematopoietic cancers: report of 19 cases and review of the literature.

Nineteen patients are reported who developed acute myeloblastic leukemia following treatment for a variety of solid tumors, including seminoma (four cases), melanoma (one case), and cancer of the ovary (six cases), colon or rectum (three cases), bladder (two cases), cervix, endometrium, and larynx (one case each). There were nine men and ten women, with a median age of 49.8 years (range 29 to 75). The mean interval between the diagnosis of solid tumors and acute leukemia is 5.8 years. In two patients the two diseases occurred simultaneously or within six months of each other. One patient was treated only surgically. Eight patients were treated with radiotherapy, five with chemotherapy, and five received both chemotherapy and radiotherapy. Pancytopenia was commonly noted prior to the onset of leukemia with chromosomal abnormalities observed in four cases in which a karyotype was performed. Three patients achieved complete hematological remission following antileukemic therapy. One hundred and six additional patients with non-hematopoietic neoplasms and acute leukemia are reviewed. Although acute leukemia may occur in a higher than expected frequency in patients with solid tumors because of a possible increased risk of a second neoplasm, it seems more likely that the acute leukemia is related to the radiotherapy and/or chemotherapy administered to treat the first neoplasm.


Ocular complications following blast transformation in chronic myelogenous leukemia.

A number of ocular problems compromising vision occurred in a patient with chronic myeloid leukemia following blastic transformation. Hemorrhagic retinopathy developed with systemic relapse and resolved with control of systemic disease. Optic nerve involvement occurred with meningeal leukemia and was controlled with intrathecal cytosine arabinoside and methotrexate. Leukemic retinal infiltrates developed despite control of systemic and meningeal disease and were successfully treated with radiation therapy. Finally, bilateral vitreous hemorrhages occurred, severely impairing vision. Leukemic infiltration of the eye may occur with increasing frequency in CML as the survival following bastic transformation improves. Infiltration should be recognized and treated promptly if serious loss of vision is to be avoided. Central nervous system prophylaxis should be considered in patients achieving a complete response following therapy for transformation.


Humoral regulation of malignant erythropoiesis.

A 4-year-old girl with acute DiGuglielmo's syndrome exhibited an unusually malignant erythropoietic proliferation as evidenced by an extremely high number of actively dividing nucleated erythroid cells in the peripheral blood and the bone marrow. The ransfusion of erythrocytes to the patient resulted in a marked decrease in the size of the liver, spleen, and the number of nucleated erythroid cells. These clinical and hematological events were associated with a reduction in the activity of plasma erythropoietin. The above findings suggested that the neoplastic nature of the erythroid elements and their lack of autonomy are not mutually exclusive.


Infantile type of chronic myelogenous leukemia in a 16-year-old boy.

Three varieties of chronic myelogenous leukemia (CML) occur in childhood familial, infantile, and adult (or ""juvenile'') types, each with distinct clinical and laboratory features. We report an unuusual case of CML with the characteristic features of the infantile type in a 16-year-old boy.


Histological and histochemical studies on the testicular cycle of a fresh water teleost Channa gachua (Hamilton).

The male reproductive cycle of Channa gachua from fresh water tanks around Dharwad (North 15 degrees 27' latitude, East 75 degrees 01' longitude) is investigated by a) recording the gonosomatic index, every month, over a period 2 consecutive years and b) studying the histological structure of the testis during this period. Based on this study, the fish is suggested to breed twice in a year. The 1st breeding phase coincides with the South-West monsoons and the 2nd with the convectional rains in the month of March. Though C. gachua shows 2 breeding periods, the spermatogenic activity is a continuous process in this fish. The presence of delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta-HSDH) and Glucose-6-phosphate dehydrogenase (G-6-PDH) is demonstrated in the interstitial tissue which indicates its steroidogenic potential.


Sedation for dental treatment of infants. I. Physiology and pharmacology.

The principal functions of the central nervous system, the pharmacology, and the effect on the central nervous system, of a number of drugs used for a sedation technique for young children are described. This information is presented as a foundation for the application of clinical procedures.


Oroantral fistula: broad-contact closure.

1. A modified Rehrmann's buccal advancement flap technique is described for the treatment of medium-sized oroantral fistulae. 2. Its advantages are listed. 3. Disadvantages are: a slightly longer operating time, and because of the longer movement of the buccal flap the buccal sulcus temporarily is shallower than the traditional buccal flap technique. 4. The buccal sulcus however, is reconstituted after a period of two months.


Susceptibility to dental caries.

The role of the pulp and saliva in caries susceptibility and resistance was evaluated by a combination of endodontic treatment and desalivation. Evidence was presented that the pulp plays a major role in caries susceptibility. Pulpal activity is modified by a hormonal factor or factors of salivary gland origin. Saliva appears to play a minor role in resistance to caries.


Dental plaque ecology related to caries and periodontal diseases.

Dental plaque consists of a dynamic microbiota which responds to ecological changes. There are major technical difficulties in obtaining representative plaque samples and in dispersing, cultivating, identifying and quantifying the microbial components. Cumulative findings on plaque microbiota associated with dental caries and different forms of periodontal disease support the specific plaque hypothesis. However, the data do not permit designation of any single organism as the distinct aetiological agent. The characteristic pattern is autogenic succesion, in which one or more microbial species alter the plaque environment and are replaced or succeeded by other species.


The metallography of heat treatment effects in a nickel-base casting alloy. A preliminary report.

A series of standard tensile specimens produced from a nickel-base removable partial denture casting alloy were subjected to heat treatments at three temperatures and three periods at each temperature. The microstructures developed within the castings disclose changes in both the matrix and interdendritic zones.


A comparison between the diametral and double-punch testing of a dental gypsum.

The tensile strength of a dental gypsum was measured indirectly using both the diametral and the double-punch tests. Observed differences between the effects of specimens size on the diametral and double-punch strengths could be consistently interpreted in terms of the statistical theory of brittle fracture. The double-punch test, when compared with the diametral test, has several important advantages. These advantages include less experimental scatter, a weaker dependence of the measured strength on the specimen size and a simpler testing procedure.


The strength of fifteen amalgam alloys.

The compressive and tensile (by diametral compression) strengths of amalgams prepared from fifteen conventional and higher copper alloys are compared and the rate at which these amalgams develop strength is investigated. Alloys of the single melt high copper type generally possess the greatest strength at all times of testing. The amalgams tend to achieve maximum tensile strength more rapidly than maximum compressive strength. The major increase in strength takes place between one hour and one day.


Childhood fear and anxiety states in relation to dental treatment.

Five years experience by the authors in organizing a dental psychology clinic has impressed on them that many excessively fearful child patients have a history of untoward dental and medical experiences which might have been prevented. A background to an understanding of fear and anxiety in children is presented, and two cases are described to illustrate straight-forward behavior modification techniques which can be used to help the fearful or excessively anxious child cope with dental treatment.


[A general roentgenographic procedure with high reliability and minimum radiation exposure].

Needs for exposure procedures in dental radiography with improved reliability and radiation hygiene, that can be applied with a minimum of time, have been documented. This publication presents a systematic approach to reliable dental radiography using beam guiding instruments which may meet such requirements. It can provide accurate radiographs in endodontic, periodontic and general practice and is adaptable to more time consuming special purpose radiography, including "serial identical" exposures and exposures of handicapped or anaesthetized patients.


[Damage from galvanic corrosion of dental alloys].

Failure analysis reveals, that using different materials for expansion screws and fixing elements may cause severe galvanic corrosion or pitting corrosion. Such failures can easily be avoided by using only one alloy with good passivation characteristics instead of different alloys.


[In vitro studies on abrasion and thickness of layers of plastic sealers].

Plastic sealers on the tooth are effective for no more than two years because of mechanical and thermal stress. Three plastics with different chemical compositions (Nuva-Seal, Alpha-Seal, and Enamite) therefore were examined in terms of abrasion. Necessary resealing results in thicker layers of sealer in the nonabrased areas, as determined with the three above-mentioned preparations. Bis-EMA type sealers satisfactorily resisted abrasion and also form the thinnest sealing layers.


[The relation of the sella nasion to the Frankfurt horizontal line in cephalometric diagnosis].

The relationship of angle SNA and SNB to angle SN-FH was determined by evaluating 106 teleradiographs. The correlation of the small SN-FH angle with the large SNA/SNB angle was statistically significant and vice versa. The normal range for SNA/SNB was determined by classifying SN-FH values. The use of these relations for the preoperative planning of orthognathic surgery was demonstrated in two clinical cases.


[Modern aspects of dental photography].

Potential areas of application for dental photography were described. The choice of the camera body depends on the intended use. The Medical Nikkor 5.6/200 and the Yashica Medical 100 lenses as well as an extension tube with approximately 100 mm focal distance may be used; the latter is best combined with a circular flash unit or one to two standard flash units. A cheek-holder and mirror are necessary. A few practical tips were presented.


Direct calorimetry in ecological energetics. Long term monitoring of aquatic animals.

The measurement of heat production represents the most general approach to the estimation of energy flow through biological systems. While aerobic energy metabolism is most conveniently studied by polarographic oxygen determination, direct calorimetry presents the only unspecific method for quantitative comparison of aerobic and anoxic metabolism in animals. In flow calorimeters constant experimental conditions may be controlled for practically unlimited periods of time, and transitions of the environmental regime may be repeatedly performed during one experiment. The merits of a direct calorimetric flow system are demonstrated in case studies of anoxic and aerobic animal metabolism and discussed in the context of current biochemical and ecophysiological concepts.


Absence of detectable mitochondrial recombination in Paramecium.

An extensive search for recombination between mitochondrial markers was carried out in Paramecium tetraurelia. Thirty-two combinations, altogether involving 24 different markers, were studied. The markers belonged to the three main categories of mitochondrial mutations presently available in this organism, (a) Spontaneous or UV-induced antibiotic resistance mutations, most probably affecting mitochondrial ribosomes, (b) nitrosoguanidine-induced antibiotic resistance markers displaying thermosensitivity or slow growth, enabling easy selection of possible wild-type recombinants, and (c) mitochondrial partial suppressors of a nuclear gene, probably corresponding to molecular alterations distinct from the preceding two categories. In addition, different genetic configurations were analyzed (i.e., mutant X mutant, double-mutant X wild-type, etc.).--None of the combinations yielded any evidence for the occurrence of recombined genomes despite the fact that: (1) all of them were studied on a large scale involving the screening of at least several thousand mitochondrial genomes (often several millions), (2) in many of them the detection level was sufficiently high to enable the isolation of spontaneous mutants in control cells, and (3) in several of them, reconstitution experiments carried out in parallel show that the conditions were fully adequate to detect recombinant genotypes. The results are in marked contrast with those obtained on the few other organisms in which mitochondrial recombination has been studied, particularly Saccharomyces cerevisiae, in which mitochondrial recombination is intense.--The most likely basis for the various manifestations of mitochondrial genetic autonomy in Paramecium, described in this as well as in previous publications, is that the chondriome of this organism is made up of thousands of structurally discrete, noninteracting units.


X-linkage of a human genetic locus that corrects the DNA synthesis lesion in tsC1AGOH mouse cells.

GM 126 diploid fibroblasts were fused with a heat-sensitive mouse cell mutant defective in DNA synthesis, and primary hybrids were selected at permissive and nonpermissive temperatures in HAT medium. Primary hybrids, primary hybrid clones back-selected in 8-azaguanine at the permissive temperature, and subclones of heat-resistant primary hybrids isolated under nonselective conditions or after 8-azaguanine treatment were tested for heat sensitivity, the expression of 26 human enzymes assigned to 19 different human chromosomes, and the presence of human chromosomes. Only the human X chromosome and X-linked marker enzymes exhibited a clear pattern of concordant segregation with the heat-resistant phenotype. On the basis of these observations, we have defined the human genetic locus that corrects the heat-sensitive lesion in tsC1AGOH as hrC1AGOH and have assigned this locus to the X chromosome. This observation provides the first instance where two selectable markers (heat resistance and 8-azaguanine sensitivity) are found on a single human chromosome and suggests that these markers may prove to be a valuable push-pull selective system of use in determining the linear arrangement of genes on human chromosomes by somatic cell genetics.


Acute myeloblastic leukemia two years after diagnosis of non-Hodgkin lymphoma.

The 18-year-old white male developed acute myeloblastic leukemia (AML) 25 months after diagnosis of poorly differentiated lymphocytic lymphoma, diffuse pattern (PDLL-D), involving cervical, supraclavicular, and mediastinal lymph nodes as well as bone marrow. Treatment of the lymphoma consisted of 2,000 rads to the mantel area and 18 months of chemotherapy with intravenous (IV) methotrexate (400 mg/m2), vincristine, and prednisone, alternating every two weeks with IV cyclophosphamide (1,000 mg/m2), vincristine, and prednisone plus monthly intrathecal methotrexate. Thereafter, a complete remission was maintained without therapy until the onset of AML. Several pseudodiploid clones containing multiple structural rearrangements and a hypodiploid clone were identified in the circulating blood at the time of diagnosis of AML. Induction therapy consisting of cytosine arabinoside, 5-azacytidine, vincristine, and prednisone was unsuccessful, and the patient died of sepsis two months after diagnosis. This case calls attention to the increased risk for subsequent acute nonlymphocytic leukemia in patients previously treated for nonhodgkin lymphoma.


Micrococcus luteus pneumonia: a case report and review of the literature.

The clinical course of a 69-year-old male with acute myelogenous leukemia is described who, while extremely leukopenic (less than 100 neutrophils/microliter) from chemotherapy, developed a cavitating pneumonia due to a gram-positive coccus, Micrococcus luteus. Aggressive antibiotic management and attainment of complete remission of his leukemia resulted in a successful outcome. A review of the literature regarding the pathogenicity of this organism and, in particular, its occurrence as a cause of pneumonia is presented.


Acute leukemia in pregnancy: transient neonatal myelosuppression after combination chemotherapy in the mother.

An 18-year-old primagravida received combination chemotherapy with vincristine, prednisone, L-asparaginase, cyclophosphamide, daunomycin, 6-mercaptopurine and central nervous system (CNS) prophylaxis with intrathecal methotrexate and whole-brain irradiation for acute lymphoblastic leukemia (ALL) beginning in the 12th week of pregnancy. Therapy resulted in sustained complete remission of the leukemia and delivery of a normally developed female infant whose immediate neonatal course was complicated by transient severe bone marrow hypoplasia. Our experience confirms the reports of others that intensive chemotherapy can be administered in the last two trimesters of pregnancy without serious teratogenic complications. However, we conclude that such therapy may cause significant myelosuppression in the newborn.


L-asparaginase effect on antithrombin-III levels.

The effect of L-asparaginase (L-asp) therapy on circulating antithrombin-III (AT-III) was monitored in three patients with acute lymphoblastic leukemia. Serial determination for AT-III by both a functional assay and an immunologic assay demonstrated a rapid decrease in serum AT-III levels, followed by a protracted recovery beyond the period of L-asp exposure. One of the three patients developed a reversible venous thrombosis of the arm. The mechanism of the L-asparaginase effect may be related to peripheral degradation of AT-III or to inhibition of production.


Hepatotoxicity in the treatment of acute lymphoblastic leukaemia.

Serial liver function tests and percutaneous liver biopsies were performed on 21 children receiving treatment for acute lymphoblastic leukaemia (ALL). The patients received continuing chemotherapy either with daily 6-mercaptopurine and weekly methotrexate or with five-day pulses of these drugs every three weeks. Liver function tests were transiently abnormal in the majority of children, but the abnormalities bore no relationship to the histology of the liver biopsy. Mild inflammatory and fatty changes were commonly seen, and early portal fibrosis was found in three out of 16 patients biopsied at between 108-130 weeks on treatment. There was no correlation between treatment regime and results of biopsy. Three patients showed possible progression of abnormalities on repeat biopsy. The risk of development of portal fibrosis appears low after 2-3 years of continuing chemotherapy, but examination of liver histology may be indicated if more prolonged therapy is contemplated.


[Radiation exposure of teeth and temporomandibular joints by radiography].

According to a report by Poretti (1975) 21,4% of all medical diagnostic radiographs in Switzerland are dental. In order to determine the associated radiation doses to patients, phantom measurements of skin and gonad exposures were made utilizing modern dental x-ray equipment and techniques. Following procedures in accordance with federal safety regulations it was not possible to measure dose values at the gonad level by single dental or temporomandibular radiographs, even by the most sensitive measuring device. 50 cumulative exposures without lead apron resulted in a range of gonad doses from 3.1 to 199 microR for dental and temporomandibular joint x-rays. Results of skin surface doses ranged from 38 to 289 mR for single dental film exposure and from 64 to 494 mR for single TMJ exposures. Consistent use of parallel technique with long cone equipment, small diaphragms, high kilovoltage and use of the lead apron guarantee great safety against radiation hazard in dental radiography.


[Use of a mounting table for arbitrary hinge transfer on an articulator].

The use of a mounting table allowing arbitrary hinge axis transfer on a semi-adjustable articulator is described. Following this method an accurate stabilization can be expected. This may prevent errors during the mounting of maxillary models.


[Patient motivation by application of an individual intensive program for obtaining oral health].

The failure of group and individual dental prophylaxis based upon an authoritarian relationship between doctors and patients is demonstrated. In a new information and motivation program, the Hygiene Intensive Program (HIP), the patient's attention is focused on gingival bleeding as an unmistakable warning symptom of oral disease. The degree of marginal inflammation is scored using the Papillary Bleeding Index (PBI). The HIP is designed to introduce trust and confidence into the cooperative efforts of the dentist and the patient. The patient can see the increasing success of the dentist's work and judge the effect of his personal oral hygiene efforts. The possibilities for the cooperation of the increasingly involved third party of the dental "Troika", the actual purchaser of dental services (health insurance, social welfare system, etc.) in the HIP are discussed.


[Gingival deflection and regeneration after electrotomy].

In hydrocolloid impressions, the visibility of the margins of cavity preparation is most important. Purely mechanical management of the gingival tissue by cotton threads does not always give satisfactory results, particularly in multiple preparations. Electrosurgery, subtly used, avoids blood and seepage from the sulcus and seems to better control the accessibility of the margins to the hydrocolloid material.


[Biomechanical aspects of maxillofacial development and their clinical significance for orthodontics].

In the present article, the authors deal with the application of biomechanics to the midfacial development and its significance in orthodontics. Research results permit to assume that the functions of position, respiration and space may act on the morphological development of the skull. This was confirmed by clinical studies.


[Physical and static-motor development of patients with clefts at ages from 0 to 10 years].

In the framework of a cross-sectional and longitudinal study, 703 anthropometric determinations were performed in 581 cleft patients aged from 0 to 10 years. The values obtained for body length, body weight and body weight and chest expansion were compared with the standard values established by Marcusson and Sälzler. The values for the circumference of the head were compared with the standard values of Nellhaus. The results show that from 87.4 to 97.2% of all these measurements of the body lie within the interval of x +/- 2 s. The growth in length is but slightly affected by the cleft. The values for body weight and chest expansion range with the lower standard values, especially in females and in patients with bilateral cheilognathopalatoschisis. There is a general trend towards circumferences of the head that are greater than the mean standard value. Developmental retardation is more often seen in females, but it is of shorter duration than in male cleft patients. Cleft patients who did not make up for retardation were afflicted with additional deformities or malformations or with mental defects.


[Growth in the width and length of the dental arch in children with unilateral cleft lip and cleft palate].

A study of casts of the dentitions of children with cheilognathopalatoschisis made over an extended period emphasizes that the development and the growth of the maxillary dental arch in width and length are considerably reduced as compared to children without clefts. Consequently, therapeutical measures must be taken to maintain the length and width of the maxillary dental arch.


[Measurements of the soft tissues in patients with cleft lip and cleft palate].

A comparative study was made on the pressure of the oral soft tissues during sequences of physiological functions and at rest in eugnathic subjects and patients with cleft lips and palates. The data obtained by means of electronic measuring instruments revealed marked differences between the two groups.


[The value of functional rehabilitation of patients with clefts for their growth and development].

The optimal rehabilitation of patients with cleft lips and palates can be realized only in the form of complete rehabilitation by the joint efforts of the oral surgeon, the logopaedist, the orthodontist and the prosthodontist. Clinical observations and studies have shown that the following three findings cannot satisfy: - imperfect functional rehabilitation of the cleft lip, especially of the bilateral cleft lip; - considerable constriction of the vestibulum, which is also relatively often seen in bilateral cleft lips; and - surgically-induced considerable flattening of the palatine vault, which restricts the use of oral removable appliances.


[New aspects of theories on the formation of clefts--current state of knowledge].

The theory on the origin of cleft lips and palates is briefly outlined with due regard to certain interesting aspects such as the discordance in monozygotic twins, the questions of noxae which might act at the embryonic period, the term of boundary line of the head of the foetus, the viewpoints of kinetic and dynamic embryology and the possible rôle of the premaxilla. Improvements in diagnosis by means of amniocentesis and foetoscopy provide starting points for preventive measures.


[Genealogic studies in children with clefts].

The genetic background of cleft children was studied at the Orthodontic Department of the Sofia Stomatological Faculty. A total of 149 members of 10 families were examined. The most-encountered microsymptoms were rotations of the lateral incisors and canines near the cleft. The results obtained from this, however, small number of subjects were not indicative of genetic factors.


[Genetic counseling of parents with children with clefts].

The elucidation of the aetiology of the individual case has proved to be of fundamental importance to the counselling interview as well as to the assessment of the risk for further affected children. Up to now, a genetic cause in the form of chromosomal or gene mutations can be assumed only if the cleft is related to a distinct syndrome. An isolated cleft obviously owes its origin to a combination of genetic and extrinsic factors. Consequently, the assessment of the risk of repetition is to a great extent based on empirical values.


[Various problems in the development of the child with clefts from the viewpoint of rehabilitative education].

Extensive studies and experience of many years evidence that optimal rehabilitation of the cleft patient may be achieved above all by early complex measures on the part of the sciences involved. To evaluate the efficiency of logopaedic early treatment in the cleft patient, the development of cleft children was studied during the first scholastic years, special attention being paid to their learning how to manage the written language (reading and spelling). A comparative study was undertaken in cleft children and children with normal speech at the beginning and during the third year of school education, assessing their discriminating performance in speech-motor, phonemic, visual, musical and rhythmical fields (modified Breuer-Weuffen test) and their performance in reading and spelling. The results obtained permitted to draw conclusions as to the early education of cleft patients.


[The necessity for logopedic-orthodontic cooperation for speech and dental development in children with clefts].

Children with cheilognathopalatoschisis exhibit a peculiar specificity of speech. The present paper deals with studies on the position of the tongue at rest, the type of respiration, the functions of the lips and the position of the tongue on articulating sounds at the second site of articulation in 35 patients with cheilognathopalatoschisis. The results obtained testify to the necessity for logopaedic orthodontic co-operation.


[Early education of children with clefts with special regard to logopedics].

Necessity for early education: --general early education goal, contents, methods and forms of organization, --special early education--rehabilitative logopaedics goal, contents, methods and forms of organization incoherent logopaedic care coherent logopaedic care instruction of parents and co-operation with nurseries and nursery schools to realize early education. realization of the rehabilitative-paedagogical early education of the cleft child.


[Various family problems of early education of early treated children with clefts].

To prevent retardation in personality development of physically and mentally handicapped children and adolescents, more attention should be paid to the qualification of parents, kindergarten teachers and teachers to educate handicapped children, and so those with cleft lips and palates. This is corroborated by findings obtained of 21 mothers of early-treated cleft children who had been involved in the present study. To qualify parents and co-educators to educate and care cleft children, they are needing sympathetic guidance by, and paedagogical advice from, all those who are contributing to rehabilitation.


Probabilities of concordance of twins with respect to genetic markers. A general formulation.

The formulas needed in the determination of monozygosity in twin pairs using genetic markers are derived and presented. A general formula for the calculation of concordance probabilities independent of gene frequencies and allele number is derived, enabling either manual computation or computer programming for any Mendelian markers usable in twin zygosity diagnosis.


The chromosomes and causation of human cancer and leukemia: XXXVIII. Cytogenetic experience in Ph1-negative chronic myelocytic leukemia (CML).

Among 300 patients with chronic myelocytic leukemia (CML) followed at our institute during the last ten years, 36 (12%) were thought to have Ph1-negative CML. In eight of these patients, chromosomal abnormalities were found in the leukemic cells; in four, the karyotypic abnormalities were established with banding techniques. The data of the present study and a review of the literature regarding chromosomal changes in Ph1-negative CML indicate that: 1) no characteristic or consistent karyotypic change is present in Ph1-negative CML and that diploidy is more common in this than any other leukemia; 2) the most common changes involve group C chromosomes (particularly +8); and 3) a missing Y is less common in Ph1-negative CML than in its Ph1-positive counterpart. The karyotypic changes in Ph1-negative CML resemble more those encountered in Ph1-positive CML than in acute myeloblastic leukemia (AML). The much shorter survival of the Ph1-negative CML patients vs that of the Ph1-positive group was again substantiated, and some of the previously reported clinical and laboratory findings unique to Ph1-negative CML were confirmed. On the basis of the cytogenetic findings it is concluded that Ph1-negative CML appears to be an entity unto itself.


The pathologic new bone formation of an osteogenic sarcoma.

The authors describe the structure of bone and tumorous new bone formation of an amputated femur affected by osteogenic sarcoma. They studied a slide of the invaded bone using stereomicroscopic and comparative histologic techniques. The bone destruction induced by the tumor produces characteristic morphologic changes both in the cortical and in the cancellous bone. Investigation of the extraosseal tumorous bone formation is described together with its relation to the cortical bone. In the development of tumorous bone plates primary and secondary formations could be distinguished. The intraosseal, tumor-induced new bone/osteoid formation appears characteristically in the invaded marrow-spaces as a so-called "miniature spongiosa". In the zone adjacent to the tumor, reactive bony spines grow out from the surface of the normal spongy plates. These findings are also analyzed from the view of the roentgenologic findings.


[Tumors of the sacrococcygeal region (author's transl)].

In the world literature is reported about 584 tumors of the sacrococcygeal region, 484 of them are analysed exactly. The symptoms of the various tumors are about the same, pain and nerval irritation were seen in the most cases. Rectal-digital examination mostly leads to diagnosis. Computertomography is of special diagnostic value since some years. Because of the localisation of the tumor therapy often is difficult. Operation is the only way to remove the tumor and should be done so radically as possible. Radiation therapy may induce malignant degeneration. Adjuvant chemotherapy till now is without of any any effect. In the paper we report about the very seldom malignant meningeoma of the sacrum, in the literature we could not find any other case. Besides this and the 584 cases of the literature there is reported about 4 other own cases of sacrococcygeal tumors, one neurofibroma and three giant cell tumors of the Os sacrum.


[Clinical data on the frequency, localization, and surgical therapy of bone tumors (author's transl)].

Between 1973 and 1978 a total of 77 patients suffering from a tumor of bone were treated and followed up at this hospital. Of these patients, 52 were found to have benign tumors and pathologic tumorlike changes of bone. 25 were found to have semimalignant and malignant tumors. This paper describes localisation of bening semimalignant and malignant tumors of bone by means of a modified WHO-classification. The surgical treatment of different bone tumors is discussed on the basis of special cases.


[Biological and physical studies on a new alloy for the demonstration of a new gamma 2-free amalgam].

Physical and biological characteristics as well as the clinical behavior of a new powdered alloy mixture (ISO 1559--1978) for the demonstration of a gamma 2-free amalgam were studied. The physical values surpassed the requirements of the Preliminary German Industrial Standards No. 13904. Compared with other gamma 2-free types of amalgam, the structure of the amalgam composed of the alloy under study contained no additional copper-containing phases which would negatively influence electrochemical events within the amalgam structure, except for the eta'-phase. Implantation tests with rabbits showed that the amalgam composed of the material under study reacted like material tested in earlier investigations which is already available.


[Follow-up study on coagulation stabilizing filling of bone defects using the new Gelastypt M after cystectomy of large mandibular cysts and after extraction of ectopic teeth].

The good results were confirmed in a follow-up examination of 23 patients two to six months after surgery. No signs of disturbed wound healing and/or inadequate organization of the clot could be observed catamnestically, clinically, and roentgenologically. The late infections often observed after implantation of Gelastypt S (with Surfen) did not develop after use of Gelastypt M 1/2. This preparation therefore may be recommended without restriction.


[Findings in hereditary dentin dysplasia].

The special characteristics of inherited dentin dysplasia were presented in two cases within one family based on clinical, roentgenographic and histologic findings. The multiple periapical findings observed in both individuals (osteitis-like structures) and dysplasia of the cement were discussed.


[Is the Furness-Moore Code applicable for computer and telex?].

The extent to which the identification of disaster victims could be improved with the code for dental findings developed by Furness and Moore (1969) was studied. This code records the most important data for a set of teeth in 12 digits.


The effect of leukemic lymphoblasts on normal granulopoiesis.

Acute lymphoblastic leukemia (ALL) in children is often characterized by defective granulopoiesis during initial and relapse stages of the disease, resulting in absolute neutropenia in vivo and in low or absent production of granulocyte-macrophage colonies in vitro. The purpose of this study was to determine if leukemic lymphoblasts from untreated ALL patients could inhibit normal granulopoiesis. Several concentrations of leukemic bone marrow cells from nine patients were mixed with either normal bone marrow cells or with autologous (HLA-identical) remission bone marrow cells, incubated for 1 hour, and co-cultured by the double layer agar technique. The cells were also cultured separately as controls. No statistically significant differences occurred between observed and expected colony counts in the majority of experiments. With three patients, slight inhibition occurred at some but not all leukemic cell concentrations tested; this inhibition was not correlated with the leukemic cell concentration. These results indicate that leukemic cells from untreated ALL patients do not significantly inhibit normal in vitro granulopoiesis at the committed stem cell level or at later levels of differentiation; therefore, such inhibition does not appear to be responsible for ALL-associated neutropenia.


Chromosomal homology of rabbit (Oryctolagus cuniculus) linkage group VI with rodent species.

Homologous portions of linkage group (LG) VI in the rabbit Oryctolagus cuniculus, chromosome 8 in Mus musculus, and LG V of Rattus norvegicus have been observed. These linkage groups in Oryctolagus and Mus contain the extension locus (e), where recessive alleles are known in many species. Preliminary linkage data have added new loci to linkage group VI of the rabbit, revised the order and map distances on the linkage map, and by comparison with rodent species have strengthened the homology of LG VI in the rabbit with chromosome 8 of the mouse and with LG V of the rat. LG VI now contains five loci with the following order and intervening map distances: Es-1, Es-2 complex--6.3 +/- 2.1 cM--Est-1, Est-2 complex--18.5 +/- 3.7 cM--e.


Resection of the distal end of the radius.

Resection of the distal end of the radius is indicated in the treatment of locally aggressive primary bone tumours, such as giant cell tumour. This requires reconstruction of the resected segment. Various techniques are examined and the one using an autoplastic fibular graft is described in detail. This method preserves good function of the wrist joint.


[Distribution and function of immunogenetic markers on epidermal cells].

Gene products of the major histocompatibility complex (MHC) are vital for the regulation of immunocompetent cell interactions and, thus, of the entire immune response. In this report, we describe the distribution of these MHC-encoded alloantigens on epidermal cells of different species and discuss their potential role in the generation of epidermal immune reactions.


Critical evaluation of commissural myelotomy in the treatment of intractable pain.

The Authors describe 6 cases of commissural myelotomy performed on patients suffering from malignant neoplasms at the Neurosurgical Department of Bergamo. A review of all cases reported in the literature has been documented by clinical data supported by anatomophysiological considerations.


Pattern and flash evoked responses in disseminated and selective optic pathway damage.

In multiple sclerosis (MS), an increase in delay in visual evoked responses (VER) may be important, but an earlier change is the behaviour of the wave-shape; amplitude is always lower even in MS patients without subjective visual symptoms, provided not only flash but also pattern stimulation is used. Normal amplitude with pattern stimulation does not necessarily mean normal amplitude with flash stimulation. Combining both stimulation methods, we found lowering of amplitude and change of wave-shape in 100 per cent of cases while latency was increased in only 77 per cent. Flash and pattern VER might be even more pathognomonic in MS than oligoclonal gammaglobulin fractionation on agar, which is positive in 75 per cent of cases. We recommend that subclinical cases of MS should be submitted to full electro-oculographic investigation. Patients with sectoral ischaemic neuropathy and open angle glaucoma (OAG) show the same findings; this emphasizes that the method is not specific for demyelinating diseases, nor selective for papillo-macular bundle involvement, but that VER, considering only its first components, reflects axonal damage somewhere in the visual pathway.


Herpes simplex virus uveitis and optic neuropathy. An experimental investigation.

The sequence of events which follows the intravitreal injection of herpes simplex virus (HSV) into one eye has been observed as it affects the eyes, optic nerves, and optic chiasm. Initially the injected (right) eye showed an intense iridocyclitis and about 16 days after inoculation an exudative retinal detachment was observed. A similar sequence of events occurred in the uninjected (left) eye about 6 days later. HSV has been shown to pass along the right optic nerve towards the chiasm and from there down the left optic nerve. The primary route of spread appears to be via neuroglial cells rather than the axons. Tissue necrosis and re-myelination have been observed.


Immunoglobulins of normal aqueous humour.

Immunoglobulin levels have been measured in aqueous humour and blood from 79 patients undergoing cataract extraction. A linear relationship between serum and aqueous IgG was found, but this was not so for IgA, and IgM was not detected in any of the aqueous samples. The morphological features of both the blood-ocular and the blood-brain-cerebrospinal fluid barriers, and the influences governing the passage of immunoglobulins across these barriers are considered. The similarity between two theories of protein penetration, diffusion through the iris root into the eye, and via the "functional leak" into the cerebrospinal fluid, are considered. We feel that the discrepancy between IgG and IgA penetrations into aqueous, despite their similar molecular weights, may be due in part to the larger hydrodynamic volume and consequently increased frictional ration of IgA, although the existence of selective IgG transfer has not been excluded.


Necrotizing scleritis after ocular surgery.

Six cases of acute necrotizing scleritis are described. These first presented after ocular surgery to the same eye and possible aetiological factors relating to the surgery are discussed. A mechanism for the production of scleritis in these cases is proposed which is analogous to an experimental animal model.


HLA antigens and certain types of uveitis.

Some preliminary results are given on human leucocyte antigen (HLA) typing of nine patients with sympathetic ophthalmitis, two with Vogt-Koyanagi-Harada disease, and nine with Behçet's disease and uveitis. In the sympathetic ophthalmitis patients the relative risk for B40 was 7.2, which would indicate that a more extensive study might be valuable. In Behçet's disease B5 and B27 both carried increased risks of 9.4 and 11.1 respectively.


Amblyopia in bilateral infantile and juvenile cataract. Relationship to timing of treatment.

Contact lenses were fitted for 51 aphakic infants. From this group data were obtained that support the hypothesis that much of the visual defect in cases of congenital cataract is due to functional amblyopia, and that it is therefore largely preventable and even treatable. The implications of the results for the management of these patients and for human amblyopia are that early treatment and optical correction are essential, since the effects of deprivation start at about 4 months of age and continue to a cumulative but decreasing degree throughout the first decade of life.


Modern simple extracapsular surgery.

A series of 444 aphakic eyes is reviewed in which extracapsular extraction was performed by modern simple instrumentation followed by the insertion of a posterior chamber lens. The visual results of 93.5 per cent seeing 6/12 (20/40) or better in an age group of 70 years plus is satisfactory. The hospitalization is short and visual recovery rapid. Complications have been few and it has been shown that peripheral iridectomy is not required in most cases as they are not subject to pupil-block glaucoma. The main problem with extracapsular extraction is the incidence of needlings of 9 per cent rising to 16 per cent with longer follow-up. Despite this it is felt that the advantages of extracapsular extraction, with rapid visual rehabilitation and a lower incidence of cystoid macular oedema and retinal detachment, outweigh the disadvantages.


Biodegradation of nylon loops of intraocular implants in children.

In none of the 418 first-stage intraocular implant insertions with intracapsular cataract extractions I have carried out in adults is there any clinical evidence of nylon loop degradation. Although there are no previous reports of clinically observable implant loop degradation, clinically detectable biodegradation was found in eleven eyes of seven children and four adults among the 34 second-stage iris-clip or iridocapsular implants I inserted after aspiration of cataract in children and young adults. The degradation has not produced any uveitis, keratopathy, or instability of the implants in any of these patients. Two children had unilateral glaucoma in the eye containing an iris-clip implant. One of them had dense amblyopia in that eye and the iris-clip implant was removed at the time of trabeculectomy. Scanning electron microscopy of this implant is reported. Active search for nylon particles in the trabeculectomy specimen of the second child has shown the presence of strongly birefringent particles in the trabecular tissue. The possible mechanism of biodegradation of the nylon loops is discussed.


Cyclic oculomotor palsy.

This rare condition, an oculomotor palsy which is interrupted by spastic contractions of the levator muscle and the pupillary sphincter at fairly regular intervals, had been reported in 54 patients up to 1975. Two patients presented to the authors with apparently complete unilateral ptosis but abnormal pupillary reactions prompted closer examination. Once the diagnosis has been considered only 5 minutes of careful clinical examination are necessary to confirm or reject it. During the past 4 years the authors have found nine new cases of the condition and it is clear that the diagnosis must often be missed. Details of the patients are shown and discussed in the light of Loewenfeld and Thompson's theory of the pathology. The place of surgical treatment is considered.


Long-term review of injuries to the lacrimal drainage apparatus.

A series of 57 patients with injuries to the proximal lacrimal drainage apparatus was reviewed to assess the long-term sequelae. The lower canaliculus was involved in 33 patients, the upper in ten, and both upper and lower in twelve, while the common canaliculus and lacrimal sac were each involved in one patient. It was found that either the upper or the lower canaliculus could cope independently with basal lacrimal secretions and that patients with at least one canaliculus intact had no symptoms under normal conditions. In seven patients with lower canalicular lacerations it was found that spontaneous functioning fistulae had developed nasal to the site of canalicular obstruction. The management of canalicular lacerations is discussed and it is recommended that the function of an intact canaliculus should not be jeopardized by surgical manipulations in an attempt to repair a damaged fellow canaliculus.


Genesis and therapy of dacryocystitis neonatorum.

By means of post mortem dacryocystograms and photomicrographs of histological slides, the topography and morphology of the lacrimal ducts in newborn infants are demonstrated. Because of bends in the course of the lower tear duct a percentage of probings will fail to effect satisfactory perforation of Hasner's membrane. In most cases the soft texture of this membrane requires no more than syringing under pressure, using the hollow probe as described by Bangerter (1953).


Preretinal macular fibrosis.

The clinical features of preretinal macular fibrosis are reviewed, particular consideration being given to related pathology at the vitreo-retinal interface. These observations are correlated with recent studies of the ultrastructural abnormalities present in this condition.


Surgical management of epiretinal membranes.

Cellular membranes growing on the inner retinal surface occur in various ocular disorders, and modern methods of vitreous surgery permit removal of these membranes in selected cases. The surgical treatment of epiretinal membranes complicating proliferative retinopathies and rhegmatogenous retinal detachment is briefly described, and the results of such surgery in a series of thirteen consecutive eyes with macular pucker after otherwise successful surgery for rhegmatogenous retinal detachment are presented in detail. A technically successful result with visual improvement was achieved in each of the thirteen eyes. An iatrogenic retinal tear (successfully repaired) occurred in one eye, and recurrent retinal detachment requiring an additional operation occurred postoperatively in two eyes. Progression of lens opacities occurred postoperatively in four of six phakic eyes, and one eye later required cataract extraction. Despite such risks, and the fact that the best postoperative visual acuity achieved was 20/30, use of vitrectomy methods to remove epiretinal membranes can provide significant visual improvement in selected cases.


Ultrastructure of epiretinal membranes causing macular pucker after retinal re-attachment surgery.

The ultrastructural features of the cellular components of surgically obtained preretinal membrane in twelve cases of macular pucker which followed retinal re-attachment surgery have been analysed. A mixture of different types of cells was observed. Definite (7) or probable (1) fibrous astrocytes were present in eight cases. Definite retinal pigment epithelium was the principal cell in only two cases, and four additional cases showed probable retinal pigment epithelium. Fibrocytes and macrophages were present in five cases and blood vessels in two. Nine of the twelve specimens included internal limiting membrane of the retina.


Anterior chamber volume. Its measurement and clinical application.

Measurement of anterior chamber volume is discussed and attention drawn to a new, convenient slit-image method, which was used to study anterior chamber depth and volume before and after prophylactic peripheral iridectomy in the fellow eyes of patients presenting with unilateral acute angle-closure glaucoma. There was no significant change in depth after iridectomy but the mean anterior chamber volume increased significantly (P less than 0.01), by nearly 3 per cent. This increase was due to elimination of peripheral iris bombé which was not present in two control groups of normal eyes, one matched for anterior chamber depth and the other for age and refraction. Peripheral iris bombé may be a quantifiable factor predisposing to the development of acute angle-closure glaucoma.


Effects of therapy on vitreous fluorophotometry in diabetes mellitus.

Quantitative vitreous fluorophotometry was used to study the alteration of the blood-retinal barrier in 116 patients with diabetes mellitus of adult onset. The patients were in good stable metabolic control on injectable insulin, oral hypoglycaemics, or diet therapy. Vitreous fluorophotometry readings were abnormally high but similar in all three groups and in those with or without early background retinopathy. A higher incidence of systemic hypertension was noted in those patients requiring oral agents compared to those on insulin or diet therapy.


Psychophysical and visual evoked potential findings in hereditary optic atrophy.

24 members of families with dominant hereditary optic atrophy were investigated. Visual acuity, Goldmann perimetry, Farnsworth-Munsell 100-hue tests, and visual evoked potentials to flash and pattern-reversal were performed. All except the Goldmann perimetry showed a significant association with the presence of optic atrophy. The flash VEP showed a positive-negative-positive response in most of the affected patients.


[Endoprosthetic replacement following resections of malignant tumors of the extremities (author's transl)].

Due to advances in chemotherapy and endoprothetics, amputation is increasingly being replaced  by local resectioning in malignant tumors. While suitable implantations for bridging defects in the proximal humerus and proximal femur are available, prostheses for resectioned tumors in the knee area  have not yet been fully developed. From 1966 to 1978, 77 endoprostheses were implanted in 29 primary bone tumors, 3 malignant soft-tissue tumors and 45 bone metastases. There were 43 endoprostheses of the proximal femur, 31 endoprostheses of the proximal humerus and 3 special endoprostheses of the knee region. In most cases, eliminating the tumor radically was successful and a functional extremity was retained.


The determination of twin zygosity by means of a mailed questionnaire.

A mailed questionnaire dealing with similarity in childhood, and how often and by whom the twins were mixed up, was applied to 290 same-sex adult twin pairs. For about 75% of pairs ten genetic markers were analysed. The agreement in zygosity classification between questionnaire and blood typing was high. Irrespective of whether raw scores of discriminant function analysis was applied, the agreement was nearly 95%. When a decision tree was applied, the percentage rose to nearly 96%. Considering that probably four of the twin pairs were wrongly classified as MZ by genetic markers, the percentage rose to 96 and 98, respectively.


Meningeal involvement in leukemias and malignant lymphomas of adults: incidence, course of disease, and treatment for prevention.

As prognosis has improved over the last several years, an increasing incidence of meningeal involvement has been recognized in adult patients with acute leukemias and malignant lymphomas. In 210 patients evaluated retrospectively, the incidence of meningeal disease was 33% for patients with acute lymphocytic leukemia (ALL), 20% for patients with acute myelogenous leukemia (AML), 22% for patients with non-Hodgkin's lymphomas with an unfavorable histology (NHL), 3% for patients with chronic myelogenous leukemia (CML), and 1% for patients with chronic lymphocytic leukemia (CLL). In most patients, meningeal involvement appeared several months after diagnosis of acute leukemia, often preceding systemic relapse if bone marrow remission had been achieved before. Prophylactic treatment of the CNS was begun in eight patients with ALL or AML after bone marrow remission was achieved. Of these patients, three with ALL and one with AML were free of disease up to 2 years after diagnosis. Methods, benefits, and risks of prophylactic treatment of the CNS for adult patients are discussed in detail.


Traumatic injuries to upper permanent incisors related to age and incisal overjet. A retrospective study.

The relationship between traumatic injuries to upper permanent incisors and incisal overjet, with special reference to the age at which the trauma occurred, was studied in a sample of 1437 orthodontically untreated children aged 7--16 years. Traumatic injuries to hard dental tissues and exarticulations of teeth were recorded. Before 10 years of age, the increase of the prevalence rates was most rapid in the extreme (greater than 6 mm) overjet group in both sexes; the ratios between the extreme and normal (0--3 mm) overjet groups being 6.5/1 in girls and 5.0/1 in boys. According to the estimate, about 50% of the girls and 70% of the boys in this overjet group with injuries were affected before the age of 10 years.


Innervation of different parts of the predentin and dentin in young human premolars.

The innervation of the predentin and inner part of the mineralized dentin was studied at the ultrastructural level in ten premolars. Each tooth was divided into fifteen different parts, each containing odontoblasts, predentin and dentin. It was found that the innervation in the coronal dentin was more compact than in the root dentin. Within the coronal dentin itself, the most densely innervated area was the dentin covering the pulp horns. No nerves were identified more than 100 microns from the pulp-dentinal border. No nervous structures were found in the mineralized dentin of the root. Nervous structures which seemed to have no connection with the odontoblastic processes were frequently observed in the predentin of the root. Signs of degeneration of the nervous structures of the predentin of the root was also a common finding. The observation that the intradentinal nerves were confined to the predentin and the most pulpal part of the dentin supports the theory that surface stimulation on dentin has an indirect effect on the nerves activated by movements in the liquid of the dentinal tubules.


Oral candidosis--frequency, treatment and relapse tendency in a group of psychiatric inpatients.

Candida fungi are frequent in the oral cavity, especially in denture wearers. The infection can cause serious complications in elderly or debilitated subject. The present experimental group consisted of 81 female psychiatric patients (35--91 years of age). In addition to a clinical examination, smears from the palate, tongue and dentures (when present) were cultured on Nickerson's medium. Patients with a positive clinical and/or microbiological diagnosis were treated for 35 days with one of two gels containing 1% chlorhexidine. Following the treatment period patients with full or partial dentures took part in a maintenance program, in which the dentures were either soaked in a 0.2% chlorhexidine solution or mechanically cleaned every day for a period of 35 days. Two thirds of the patients showed clinical signs of oral candidosis or had positive cultures preexperimentally. The treatment with chlorhexidine gels resulted in negative cultures in 71% and negative clinical diagnosis gels resulted in negative cultures in 71% and negative clinical diagnosis in 96% of the patients. Mucosal soreness occurred during the treatment period in 8 out of 28 patients wearing full dentures. Xerostomia was not influenced by the chlorhexidine treatment. The relapse tendency was low during the post-treatment period. No clearcut differences between the two maintenance methods could be detected.


Radiopaque zones in the dentin beneath silicate fillings analyzed by electron microprobe.

Seven teeth which showed radiopaque zones beneath silicate fillings were sectioned and studied by microradiography and electron microprobe analysis in order to elucidate which elements were responsible for the increased absorption of X-rays and whether the presence of carious lesions could be masked by foreign elements in the tissue. The Ca concentration was frequently found to be either slightly increased or reduced compared with radiographically unaltered dentin. Increased concentrations of F and Zn occurred in the radiopaque zones. The highest F concentrations (0.4--1%) were recorded in zones with an increased Ca content. The highest Zn concentrations (5--8%) were found in the three specimens having a reduced Ca content. The possible conditions responsible for the increased Ca, F and Zn concentrations are discussed. The results indicate that both Ca and Zn may contribute to the increased absorption of X-rays. The hypothesis that carious dentin may be obscured in intraoral radiographs by the presence of foreign elements in the tissue, was not substantiated.


Evaluation of extrinsic tooth discoloration.

Extrinsic brown tooth discolorations are frequently detected in connection with antibacterial agents used for chemical plaque control. The present investigation confirmed the staining ability of an antibacterial quarternary ammonium salt, benzethoniumchloride, used as a 2% additive to a dentifrice. A weak chelator did not alter the discoloration tendency. Extrinsic discoloration was evaluated both by direct inspection, from color photographs and by using a microphotometric technique. The discoloration tendency was well demonstrated by all three techniques. Evaluation from the color photographs, however, may be preferable in epidemiological surveys while the microphotometric technique may possess certain advantages in detailed experimental discoloration studies.


Nature of transformation of hydrocortisone by cells incorporated into polyacrylamide gel.

The ability of Mycobacterium globiforme 193 cells immobilized in polyacrylamide gel (Paag) to transform soluble and microcrystalline hydrocortisone was investigated under conditions to periodic aeration. It was found that the specific 3-oxosteroid-delta'-dehydrogenase activity of immobilized cells hardly differs from that of free cells and averages 0.06 mumole/mg cells . min; the quantitative and qualitative composition of the transformation products formed by free and immobilized cells was identical. The linear relation between the accumulation of the reaction product, prednisolone, and the transformation time, the absence of an activating effect of SAA [surface-active agents] on the 3-oxosteroid-delta'-dehydrogenase activity, the correlation between enzymatic activity and viability, and the absence of an increase in activity during repeated transformations indicate that the cytoplasmic membrane of Myc. globiforme is not a significant diffusion barrier for the reaction substrate and product. Maintenance of immobilized cells in a viable state is a necessary condition of the retention of enzymatic activity by these cells. "Activation" of immobilized cells is achieved by incubating the granules in nutrient medium. The cause of the "activation," in our opinion, is the appearance of a new surface and, probably, internal cell population.


[Diagnostic and therapeutic problems in tumors of the bones of the hand].

The diagnosis and treatment of tumors of the skeleton of the hand are reviewed. Benign enchondromas were found to be the most common. Primary osteogenetic sarcoma was rare as was malignant degeneration in initially benign tumors and in the bony manifestations of systemic disease such as neurofibromatosis or hyperparathyroidism. Tumors were more common in phalanges and metacarpals than in the carpus and showed their highest incidence in the 4th decade. Diagnosis was aided by angiography and bone-scanning. Some difficulties were encountered in pathological examination. Treatment involved curettage and cancellous grafting in small tumors, en bloc resection followed by osteosynthetic stabilisation in larger instances and radical ablation followed by chemotherapy in malignancy.


The prevalence of periodontal diseases iin urban Israeli youth (14-18 years of age).

The purpose of the present study was to obtain current information regarding periodontal diseases. The study was conducted in Israel among ninth to twelth grade students, 14 to 18 years of age, residing in Jerusalem, Tel Aviv and Ramat Gan. A total of 725 school children were examined. During the examination only periodontal status was checked. Periodontal status was recorded according to Russell's Periodontal Index (PI). The mean PI found was 0.52. The results indicate an increase in the mean PI with age. Significant difference was found in the mean PI values between girls and boys. Girls constantly scored lower mean PI values than boys. No significant differences were found in the mean PI scores when studied according to country of origin.


A temporary-permanent splint.

A method of temporary permanent splinting has been presented which enables the clinician to stabilize anterior teeth aesthetically and rigidly without resorting to cast restorations. The method utilizes an acid-etch composite technique with T.M.S. Pins and has shown itself to be a rapid, cosmetic and durable alternative to either the temporary intra-coronal wire ligation or the more permanent gold prosthesis.


Gastro-intestinal haemorrhage associated with leukaemic infiltration of the stomach: A report of 2 cases.

Two cases of leukaemic infiltration of the stomach are presented. In both patients the dominant presenting sign was gastro-intestinal bleeding. The clinical, radiological and pathological findings are presented together with a brief review of the literature.


Polyploidy, plants, and electrophoresis.

Investigations of polyploidy using electrophoresis are at present severely limited by several areas of difficulty which limit all applications of this technique. The technical problems inherent in electrophoresis of plants, namely extraction of active extracts and maximizing resolution of electrophoretic variants through investigation of gel and especially assay conditions, have never been addressed explicitly. No rationale for initiating work on a new species is available. Analytical approaches to defining the conditions which limit resolution are rare. Gel-to-gel variation is poorly controlled, seldom monitored. The individual nature of electrophoretic investigations limits the comparability of data among labs. In short, "hit-and-miss" approaches predominate, and these limit investigation. Techniques are now available which one can hope will greatly improve the experimental situation.


[Jaw reconstruction with fixation plates].

The use of internal fixation plates in the reconstruction of mandibular continuity following serious injury, or resection of mouth floor tumours, is described. When complicated fractures are involved, the combined employment of osteosynthesis with steel wires and fixation plates is advised. Three illustrative cases from a personal series are presented.


[Anatomo-radiological evaluation of the long term results of surgery of large odontogenic cysts].

Long term surgical results in 47 cases of great size odontogenic cysts are examined with an anatomo-radiological method. The reparative osteogenetic process of these great cysts with mandibular localization are compared with those with maxillary localization, following anatomo radiological and clinical-radiological methods. These results are also examined according to the surgical techniques used.


[Preventive therapy in stomatological and maxillofacial surgery. Clinical considerations on the administration of cephaloridine, using a new technic (short-term prophylaxis)].

The "Short term prophylaxis" technique was used in 100 patients who had undergone Stomatologic surgery and Maxillo-facial surgery for post-operative prophylaxis of surgical infections according to analogous researches conducted in other surgical branches by various authors. The results obtained can be summarized as following: 1) a marked reduction in administered antibiotics (cefaloridina); 2) a marked reduction of post-operative fever; 3) a maintainment of average febrile levels at 37,8 degrees-38 degrees. The above tecnique thus allowed a more rapid mobilization of patients who had undergone surgical intervention, a reduction of the recovery period and the therapeutic costs.


[Use of a new cobetalactamine in the therapy of acute and chronic odontogenic processes].

Treatment of 70 patients with acute and chronic odontogenic processes for 5-35 days (mean 10.9 days) with a new cobetalactamine is reported. Rapid resolution was noted in nosological groups marked by the presence of acute infection. Particular interest is attached to the effect of the drug on chronicisting infections, such as apical granuloma, maxillary osteitis, and radicular cysts, since these forms displayed a high degree of vascular isolation, were often sustained by a mixed flora, and had been previously treated with other antibiotics. Follow-up four weeks after the conclusion of treatment showed a low incidence of recurrences that was in all events lower than in earlier experiments with chemical antibiotics. There were no instances of intolerance or allergy.


[An unusual case of generalized amyloidosis with localizations in the oral cavity].

After a brief examination of the most modern conceptions relating to amyloidosis desease a case of sistemic amyloidosis localized in the oral cavity is presented by the authors. An iconografic series of the lesions affecting the mucous membrane of the lips, checks and tongue is meant to contribute to an easier and immediate finding of analogous cases marked by a primary localization in the oral cavity, thus facilitating an early diagnosis of the sistemic desease.


[Myxomas of the jaws. 10 cases and differential diagnostic evaluations].

Ten cases of myxoma of the jaw bones are presented. After an examination of the anatomical-clinical-radiological aspects and the micro- and macroscopic pathological anatomy, the authors present a histopathological differential diagnosis and in particular demonstrate that a diagnosis of myxoma is based upon its localization and the exclusion of any benign and malignant lesions due to myxoid. Conservative excision is the preferred treatment.


[Death after a dental infection].

The pertinent features of life-threatening complications of dental infections have been briefly reviewed with particular emphasis on the alterations of the clinical features of these conditions induced by antibiotic therapy. The clinician who deals with dental infection must exercise a high index of suspicion to consistently abort the development of these complications, especially when treating debilitated patients or individuals with compromised immune functions.


[Use of chlorhexidine in the therapy of some stomatological diseases].

A mouthwash or toothpaste containing chlorhexidine gluconate was used to treat spontaneous or provoked bacterial plaque, primary or postoperative secondary gingivitis, and aphthae in 74 male and female subjects (mean age: 37 yr). As observed by other authors the antiseptic was very effective in the prevention of plaque, the healing or primary gingivitis, and the cicatrisation of surgical wounds. It also accelerated the regression of recurrent aphthae.


Sulcal prominence in young chronic schizophrenic patients: CT scan findings associated with impairment on neuropsychological tests.

Four of 17 chronic schizophrenic (or chronic schizoaffective) patients between the ages of 20 and 35, in partial remission, and living in the community were found to have mildly or moderately prominent sulci, as disclosed by computerized tomography (CT scan). These four patients were matched for sex and age with four chronic schizophrenic (or schizoaffective) patients without sulcal prominence. The Halstead-Reitan battery (HRB) of neuropsychological tests, including the Wechsler Adult Intelligence Scale (WAIS), was administered to these eight patients with the examiner blind to results of the CT scan. Each of the four patients with sulcal prominence considerably exceeded an established cutoff score for brain impairment on the HRB, whereas only one of the control schizophrenics performed in the impaired range, and that just beyond the cutoff. The WAIS Verbal IQ, mneasured concurrently, was in the normal range for all subjects and similar for the two groups. These findings suggest that mild or moderate sulcal prominence on CT scans among young chronic schizophrenics is associated with impaired neuropsychological functioning.


[Prosthetic rehabilitation with a force-distribution prosthesis. Clinical and statistical evaluation of the results obtained in 79 oral rehabilitations].

The results obtained with 79 odontoprosthetic rehabilitations by means of removable force distribution prosthesis are reported. Using half-round section wires, this prosthesis distributed masticatory forces axially on the abutment teeth and also distributes them evenly over the edentulous crest, thus achieving a mixed dental and mucous support. Clinical and statistical assessment takes in 79 patients of both sexes aged between 22 and 69 treated over the fifteen year period since 1964. All types of edentulism according to the Kennedy classification were treated. The clinical results were considered, particularly the periodontal situation and the mechanical results, above all friction between retention and function elements of the connecting wires. Clinically, 82,2% of results were positive and 74.6% of the results were mechanically positive also.


An electrophysiologic indication of defective information storage in childhood autism.

We used a combined behavioral and electrophysiological technique to test the hypothesis that storage of information is impaired in childhood autism. Endogenous event-related potentials associated with the random deletion of stimuli within a regular train of auditory or visual stimuli were examined in three autistic and three normal children. We found that all subjects were able to detect the stimulus deletions, but cortical potentials associated with stimulus omissions were smaller or absent in the autistic subjects. These results are consistent with dysfunction within the system that includes posterior parietal cortex and its connections with the mesolimbic temporal cortex and hippocampus.