Species-specific suppression of histone H1 and H2B production in human/mouse hybrids.

Ten human/mouse hybrid cell lines that segregate either human or mouse chromosomes were examined for the expression of human- and mouse-specific histones H1 and H2B. Results of this study indicate that the human and mouse chromosomes in hybrid cells that segregate human chromosomes (M greater than H hybrids) contain only mouse histone H1 and H2B. Chromosomes in hybrid cells that segregate mouse chromosomes (H greater than M hybrids) contain only human H1 and H2B histones. Loss of the ability to produce either human or mouse histones does not seem to be due to the loss of specific human or mouse chromosomes because M greater than H hybrids retaining at least one copy of each human chromosome contain only mouse H1 and H2B and H greater than M hybrids retaining at least one copy of each mouse chromosome contain only human H1 and H2B histones. These results, together with those concerning histone H4 acetylation levels and ratios of variants of histones H3 and H2A that are like those in the dominant parent cell type, indicate that the control mechanisms affecting H1 and H2B expression in H greater than M and in M greater than H hybrid cells affect expression of histones H2A, H3, and H4 genes as well. The present data thus support the hypothesis that none of the histone genes that are active in the recessive parent cell type is expressed in hybrid lines that segregate recessive cell chromosomes.


Genetic alterations in potassium transport in L cells.

Starting with mutagenized cultures of the mouse fibroblastic cell line LM(TK-), we have selected mutant clones by their ability to grow at 0.2 micrometer K+, a concentration unable to support the growth of the parent cell. The mutants fall into two classes on the basis of their potassium transport properties. Both classes maintain a high intracellular K+ concentration when growing in low-potassium medium, and both are unaltered in the ouabain-sensitive Na/K pump. One class shows an increased activity of a ouabain-resistant, furosemide-sensitive K+ transport system; the other class shows a decreased activity of a specific component of K+ efflux.


Cytoplasmic transfer of resistance to antimycin A in Chinese hamster cells.

A mutant subline of V79 Chinese hamster cells resistant to antimycin A (ANT) was obtained by treatment with ethyl methanesulfonate followed by serial selection in ANT-containing medium. Clonal derivatives of the resistant line are less susceptible than parent cells to growth inhibition by ANT in mass populations and show a higher plating efficiency in graded levels of inhibitor. Resistance to ANT is stable in drug-free medium and follows a pattern of incomplete dominance in hybrids between resistant and sensitive cells. No cross-resistance to chloramphenicol or other unrelated compounds was observed. Cytoplasmic transmission of ANT resistance can be readily demonstrated by fusing enucleated cytoplasts from resistant donors to sensitive recipient cells. The resulting cytohybrids ("cybrids") grow selectively in ANT but are identical karyotypically with the recipient cell type. As a putative mitochondrial marker, ANT resistance may be usefully combined with chloramphenicol resistance for studies on segregation and recombination in the mitochondrial genome of mammalian cells.


Model for evolution of Y chromosomes and dosage compensation.

Some difficulties with the classical model for the evolution of a genetically invert Y chromosome are discussed. An alternative model is proposed, which is based on the principle of Mullers ratchet; this involves the accumulation of chromosomes bearing deleterious mutant genes in a finite population in the absence of crossing-over. This process would result in the gradual increase, with time, in the number of mutant loci carried in an average Y chromosome, although the frequency of individual deleterious alleles at most loci remains low. It is shown that this creates a selection pressure for differentially increasing the activity of the X chromosome in heterogametic individuals at the expense of that of the Y, leading eventually to a genetically inert Y chromosome and to the evolution of dosage compensation.


Location on chromosome 1 of Rnr, a gene that regulates renin in the submaxillary gland of the mouse.

Renin activity (EC 3.4.99.19) was measured in submaxillary gland extracts from four sets of recombinant inbred mouse strains. Recombination between Rnr, a gene that mediates the susceptibility of submaxillary gland renin to induction by androgen, and Dip-1, a chromosome 1 marker, was found in only 1 of 51 recombinant inbred strains, indicating that the two genes are closely linked on chromosome 1. Renin activity in androgen-treated female mice of all recombinant inbred strains resembled that of one or the other progenitor strains, as expected if a single gene is responsible. Documentation that a single gene can have major effects on renin in the submaxillary gland of the mouse implies that single gene differences might explain known variations in renin in other species.


Polymorphism of DNA sequence adjacent to human beta-globin structural gene: relationship to sickle mutation.

Restriction endonuclease mapping of the human globin genes revealed a genetic variation in a Hpa I recognition site about 5000 nucleotides from the 3' end of the beta-globin structural gene. Instead of a normal 7.6-kilobase (kb) fragment which contains the beta-globin structural gene, 7.0-kb and 13.0-kb variants were detected. Both variants were found in people of African origin and were not detected in Asians or Caucasians. The 13.0-kb variant is frequently associated with the sickle hemoglobin mutation and may be useful for the prediction of the sickle cell gene in prenatal diagnosis. Polymorphism in a restriction enzyme site could be considered as a new class of genetic marker and may offer a new approach to linkage analysis and anthropological studies.


Molecular nature of the complement lesion.

The principle molecular event leading to membrane perturbation by complement is the assembly of the terminal five serum complement components (C5b-C9) into a macromolecular C5b-9 complex on the target membrane [Müller-Eberhard, H.-J. (1975) Ann. Rev. Biochem. 44, 697--723]. The present communication reports on the ability of purified C5b-9 complexes isolated from target membranes to become reincorporated into artificial lipid vesicles. The data indicate that the complex is a vertically oriented, hollow, cylindrical macromolecule possessing lipid-binding regions that enable one terminus to penetrate into the lipid bilayer. A transmembrane pore appears to be created at the attachment site of the C5b-9 complex.


Steady-state analysis of tracer exchange across the C5b-9 complement lesion in a biological membrane.

Resealed erythrocyte ghosts have been used to define the kinetics of tracer exchange across the membrane-bound terminal complex of the complement cascade (C5b-9). Under steady-state conditions and at net chemical equilibrium, C5b-9 ghosts showed no significant lysis above control levels as measured by hemoglobin efflux. In 1 mM sucrose at 37 degrees C, [14C]sucrose isotopic exchange diffusion into C5b-9 ghosts occurred at 4.8 (+/- 0.5, SEM) X 10(-20) mol sec-1 per functional lesion, equivalent to an apparent permeability coefficient of 4.8 X 10(-14) cm3 sec-1 for the single C5b-9 lesion. No significant uptake of [14C]sucrose above control levels was observed in C5b67 ghosts. The apparent rate of tracer permeation through the complement lesion is one to two orders of magnitude slower than predicted by a model of a transmembrane channel of dimensions permitting free diffusion of sucrose. The data support earlier assertions from this laboratory that diffusion of small molecules across the complement lesion in biological membranes is significantly restricted.


Characterization of immunoglobulin from the Pacific hagfish, a primitive vertebrate.

Antibodies to group A streptococcal carbohydrate, demonstrable by radioimmunoassay, were elicited in Pacific hagfish, Eptatretus stoutii, after prolonged immunization with whole cell vaccine. This hagfish antibody, isolated by ion-exchange chromatography of immune serum, exhibited a single precipitin line after immunoelectrophoresis against rabbit antiserum to hagfish serum. The isolated antibodies retained high binding activity for the streptococcal carbohydrate, but lacked the natural hemagglutinating activity found in both nonimmune and immune sera. Analysis by gel filtration in neutral and dissociating buffers and by sodium dodecyl sulfate/polyacrylamide gel electrophoresis under nonreducing conditions indicated an intact molecule of molecular weight approximately 160,000 that may be noncovalently associated in a polymer of higher molecular weight. Heavy chains of mobility identical to that of murine mu chains and light chains of mobility significantly slower than murine lambda chains were obtained after sodium dodecyl sulfate gel electrophoresis under reducing conditions. Instability of the tertiary structure of the antibody molecule was indicated by partial dissociation in buffers containing sodium dodecyl sulfate and by dissociation at low concentrations of reducing agent. In contrast to its well-developed system of cell-mediated immunity, only a minimal system of circulating antibody production is evident in this primitive fish. No evidence for divergence of cyclostome lymphocytes into separate T- and B-cell systems has yet been discerned.


Self-nonself concept for cancer and diseases previously known as "autoimmune" diseases (illegitimate transferases/plasma exchange).

The illegitimate glycosphingolipid antigens of the P blood group system and of the Forssman (Fs) tissue antigen in adenocarcinoma which are foreign to the host suggest the self-nonself concept which applies also to numerous other diseases such as rheumatoid arthritis, lupus, gluomerulonephritis, and idiopathic acute hemolytic anemia. In the presence of the glycosphingolipid antigens such as ABO, P, and Fs, the normal serum of the homozygote recessive precursor contains antibodies for the missing antigen(s). The expected antibody to the Fs antigen was present in about 75% of normal men and women. In cancer sera, the incidence of anti-Fs was decreased to about 35-40%. On testing the normal population anti-Fs was present in 90% of the sera in the youngest group, and this value gradually diminished in the older groups; the incidence of the antibody in the 70-year age group was to about 60%. The rate of loss of anti-Fs with increasing years appears to parallel the gradual loss of anti-A and anti-B isoagglutinin titers. This phenomenon may be associated with the gradual diminution of protein synthesis with aging or the continuous accumulation of soluble immune complexes in the serum, or both. It is suggested that the self-nonself concept is also the basis for the pathogenesis of rhematoid arthritis, lupus erythematosus, idiopathic acute hemolytic anemia, and numerous other conditions classified as "autoimmune" diseases. Some of these diseases are induced by viruses or drugs or both. When a virus or drug attaches itself to the membrane of a tissue cell, the self is converted to nonself which, in rheumatoid arthiritis, alters its self Ig to nonself Ig.


Characterization of a nontrypsin cholecystokinin converting enzyme in mammalian brain.

An enzyme has been partially purified from canine and porcine cerebral cortical extracts that differs from trypsin in that it manifests some degree of hormone specificity since it converts porcine cholecystokinin to smaller immunoreactive forms, i.e., the COOH-terminal dodecapeptide and octapeptide fragments, but fails to convert big gastrin (34 amino acids) to heptadecapeptide gastrin. This enzyme is distinguishable from trypsin not only in substrate specificity, but also in several physiochemical properties. It is not inhibited in the presence of concentrations of lima bean trypsin inhibitor sufficient to inhibit 1 mg of trypsin per ml of incubation mixture. It is inactivated when incubated with substrate at 45 degrees C for 1 hr, whereas trypsin remains fully active when incubated under the same conditions at 55 degrees C. The enzyme elutes in the void volume on Sephadex G-50 and G-75 gel filtration. On sucrose gradient centrifugation, the proteolytic activity associated with trypsin is recovered above albumin but that of the solubilized brain enzyme is recovered below gamma globulin. The enzyme is not detectable in splenic extracts, which do contain nonspecific proteases capable of completely degrading cholecystokinin. Further investigation is required to determine whether the enzyme in the gut that converts cholecystokinin to the bioactive and immunoactive COOH-terminal fragments resembles or is different from the brain converting enzyme.


Generation of b-wave currents in the skate retina.

Potassium kinetics within the skate retina were monitored extracellularly with K+-selective electrodes. Two sources of K+ efflux were detected in response to photic stimulation: one in the distal retina in the region of the outer plexiform layer, and the other at a more proximal location near the border between the inner nuclear and inner plexiform layers. The magnitude of the K+ efflux at these retinal depths was affected differently by spot and full-field illumination, suggesting that the two sources originate from different classes of neuron. There is evidence that both sources are associated with current sinks provided by the Müller cells, thereby establishing radial current paths along the lengths of these elements. We have proposed a model in which asymmetries in the magnitudes of these currents give rise to the b-wave of the electroretinogram. Extracellular field potentials recorded differentially at various retinal depths, and in response to changes in stimulus configuration, were consistent with predictions of the model.


Evidence for mediation of a neuronal interaction by a behaviorally active peptide.

Egg laying hormone, a peptide neurohormone with an approximate molecular weight of 6000, was isolated from the region of the abdominal ganglion of Aplysia that contains the neuroendocrine bag cells and purified by gel filtration chromatography, isoelectric focusing, and dialysis. A 1-min local application of egg laying hormone to the identified neuron R15 produced prolonged (greater than 1 hr) augmentation of impulse activity in this neuron. The distinctive quality and prolonged duration of the response are apparently identical to the previously described response to electrically elicited bag cell activity. The results provide evidence that egg laying hormone is the mediator of this prolonged neuronal interaction.


[Gardner's syndrome (author's transl)].

Gardner's syndrome is an association of rectopolyposis, to which the prognosis is related, with bony lesions : osteomas, essentially craniofacial, and osteocementosis : dental : multiple inclusions, supernumerary teeth; and cutaneous manifestations : lipomas, adenomas, fibromas ; and sometimes desmoid tumours, either cutaneous at the site of an abdominal scar, sometimes mesenteric. This complex histodysplasia, subject to the possibility of complications where it occurs (in particular craniofacial osteomas) requires digestive surveillance and further treatment when necessary : colectomy with colo-rectal anastomosis associated with coagulation diathermy of rectal polyps. When the latter is impossible, abdominoperineal excision of the rectum is followed by permanent ileostomy.


[Cyst of the nostril (author's transl)].

Cysts at the edge of the nostril develop from the remains of the antero-inferior prolongation of the lacrimonasal duct, in approximately 98% of cases in the woman. It is a rare condition, the diagnosis of which is easy if the practitioner has ever seen a case. Its clinical appearance is, in fact, very uniform, characterised by the appearance and increase in size of the extra-osseous neoplasm, with extension into and under the nostril. Excision is easy and should be performed by a buccal approach.


[The equivalent of the Morel-Lavalle syndrome in the cheek associated to a fistula of Stensen's duct (author's transl)].

The authors report a case of post-traumatic jugal lymphatical effusions associated to a fistula of Stensen's Duct. The lesions have healed by placing a suction drainage without modelling catheterism.


[Osteochondroma of the mandibular condyle. Case report (author's transl)].

A rare case of osteocartilaginous exostosis, also called osteochondroma, is described. The resection of the tumor through an extra-oral incision is the treatment of choice. The follow-up covers a period of 4 years. No relapse was observed and postoperatively the function of the temporomandibular joint was practically normal.


[Surgico-orthodontic recovery of horizontal impacted second and third lower molars (author's transl)].

In 6 cases of horizontal impacted second molars seen between 1951 and 1961, the authors were able to remove or ensure removal of the gum overlying the crowns of these teeth, to make a small transverse horizontal hole below the mesial neck, and straighten them in an average period of 5 months with the use of an auxiliary spring on a plate or better on a vestibular arch (figs 5 and 6). The constant success of this method led them to use if for impacted lower wisdom teeth, even when almost horizontal (figs 1, 2, 3, 4) in patients aged between 17 and 20 years, avoiding their removal.


[The advantage of marginal glossectomy (author's transl)].

The marginal glossectomies may be extended without disadvantage to the whole margin of the tongue and give a reduction in the volume of the tongue on the margins and, in some cases, before.


[Technique of glossectomy (author's transl)].

Glossectomy is performed in order to reduce the size of the tongue. There are four types:--median anterior glossectomy, the most classical being median lozenge glossectomy;--central glossectomy which does not touch the peripheral border of the tongue;--glossectomy combining these two methods;--marginal glossectomy which may be segmental or extended to involve the whole of the perimeter of the tongue.


Acute and protracted effects of vinblastine on odontoblasts and dentinogenesis in rat incisors.

The effects of a large dose of vinblastine sulfate (2 mg/kg body weight) on proliferating odontoblast precursors and secretory odontoblasts in the continuously growing rat incisor were studied. The rats were killed 6 h, 24 h, 3 d and 7 d after vinblastine injection. Most cells in the proliferating zone contained arrested mitoses, or had perished after 24 h. After 3 and 7 d, the odontoblasts derived from this zone were reduced in number, and showed altered cell shapes. The odontoblasts had produced irregular dentin. The secretory odontoblasts had displaced nuclei and altered cell shapes after 24 h. Those most affected were opposite early mineralized dentin. In some incisors the cells had perished. In the protracted experiments almost all the odontoblasts were changed and had produced abnormal dentin. In the early mineralized dentin area, accumulations of cells were present after 3 d, and osteodentin-like material after 7 d.


Histochemical characterization of alkaline phosphatase in developing rat teeth and bone.

Alkaline phosphatase (EC 3.1.3.1.) in developing teeth and in bone has been studied. Prior to hard tissue function a rather high enzyme activity was noted in differentiating odontoblasts, stratum intermedium, and outer enamel epithelium. A lower activity was observed in the cells of the dental papilla and stellate reticulum. After the onset of hard tissue formation the alkaline phosphatase activity was generally increased. Enzyme activity was also found in the proximal part of tall, secretory ameloblasts. In the short postsecretory ameloblasts a high enzyme activity was noted. At the onset of dentin mineralization there was an increase in enzyme activity in the cells of the subodontoblastic layer. In bone the highest alkaline phosphatase activity was found in osteoblasts. A difference was noted between the alkaline phosphatase of hard and soft tissues by means of the addition of inhibitors to the incubation media. Within the hard tissues it was possible to distinguish between two alkaline phosphatases after pretreatment with heat (56 degrees C) or the addition of specific inhibitors (sodium metavandate, ortho-and pyrophosphate). An isoenzyme which was sensitive to these procedures was demonstrated in odontoblasts and in the pulpal connective tissue. Another alkaline phosphatase isoenzyme, which was resistant to pretreatment with heat or the addition of vanadate or phosphate, was demonstrated in the subodontoblastic cell layer, stratum intermedium and the outer cells of the reduced enamel epithelium.


Effect of vincristine on odontoblasts in rat incisor.

Vincristine in doses of 0.1, 0.3, 0.5 and 0.7 mg/kg was administered to 60 rats in four groups. Histomorphologic investigation of the odontoblast population in the maxillary incisors revealed dose-dependent reactions consisting of (1) swelling of the odontoblasts and an accumulation of abnormal mitotic cells in the germinative parts of the pulp after 5 h, (2) a supervening necrosis and destruction of some odontoblasts and of the mitotic cells after 24 h, and (3) after 3 d, a reversal to normal structure in some parts of the odontoblast population, but a further development of the vincristine-induced changes, with severe cellular derangement and irregular predentin production, in others.


Hereditary fibrous hyperplasia of the gingiva with varying penetrance and expressivity.

A kindred affected with hereditary fibrous hyperplasia of the gingiva with varying penetrance and expressivity is described. Most of the affected members were short in stature. The pedigree strongly suggested an autosomal dominant mode of inheritance. The penetrance of the trait varied, and three unaffected persons had transmitted the disease to their offspring. There were two modes of expression. The mild form showed only bilateral rugose thickening of the palate, whereas the severe form showed gingival hyperplasia in addition to changes in the palatal mucosa. The changes were already present at birth. Histological examination showed large amounts of alcian blue-positive extracellular material in the subepithelial connective tissue of the hyperplastic mucosa. Loose collagen-poor connective tissue with alcian blue-positive extracellular material and dense collagen-rich connective tissue in the rugose projections of the palatal mucosa were often segregated.


Clinical and radiographic study of pulpectomy and root canal therapy.

A clinical and radiographic reexamination of 2,459 roots 2-7 years after initial pulpectomy or root canal therapy is presented. The overall success rate, which was 53%, was not affected by the sex or age of the patient, or by the jaw in which the tooth was situated. The tooth group, however, had a significant influence on the success rate, the worst results being obtained for incisors and especially the mandibular central and maxillary lateral incisors. The prognosis was clearly better for the pulpectomies than for the root canal therapy. Mortal pulpectomy was found to succeed more often than vital. The presence of a primary periapical rarefaction worsened the success rate. The success rate was lower for the teeth in which a posttreatment prosthetic crown had been fitted. Fillings which went beyond the apex had a significantly lower success rate than those which nearly or exactly reached the apex.


Electron microscopy of exfoliated cells of human oral mucosa.

Exfoliated cells of the human buccal mucosa were studied in 35 adults. All cells showed signs of necrosis: condensation of nuclear chromatin, dilated mitochondria with flocculent densities, dilation of endoplasmic reticulum, ribosomes detached from the endoplasmic membranes, aggregates of degenerated organelles in the cytoplasm, and discontinuity of nuclear and plasma membranes. Desmosomes were preserved in cells exfoliated in groups, but in single cells the former sites of desmosomes were only occasionally identified. The cells contained filaments about 150 A in diameter, often arranged in bundles around aggregates of degenerated organelles. Ruthenium red stained the glycocalyx of the epithelial cells and the capsule of accompanying bacteria. The glycocalyx did not appear to contribute to binding the bacteria to cell surfaces. The findings are discussed in the light of recent studies on cell necrosis, and culminate in a hypothesis to explain exfoliation of cells of the oral epithelium.


Barrier properties of inflamed denture-loaded palatal mucosa to water.

The barrier property of inflamed palatal mucosa to water has been studied in eight adult edentulous persons with a generalized denture stomatitis, by measuring the transmucosal water flow under varying osmotic gradients. Flow rates were registered gravimetrically in solute saturated filter paper discs after 10-min periods of mucosal contact, using solutions with an osmolarity of 0, 0.25, 0.30, 0.38, 0.50 and 0.75 osmol sucrose/l. The histology of the mucosal areas was evaluated from cytologic scrapings, and biopsy material from two persons. The inflow with use of pure water was 2.98 mg/cm2/10 min, being about three and a half times greater than through the intact mucosal surface. The point of isotony of the inflamed mucosa ranged between 0.30 and 0.36 with a mean value of 0.33 osmol/l, thus being of the same magnitude as in plasma and tissue fluid. The observations from the biopsy material were in accordance with earlier histological evidence from generalized denture stomatitis, indicating that the permeability properties of the inflamed mucosa belonged to epithelial cell layers located in the lower part of the spinous layer.


Experimental Candida-induced denture stomatitis in the Wistar rat.

An experimental model of yeast-induced denture stomatitis has been set up in the rat by inoculating Candida albicans on the fitting side of a maxillary acrylic plate retained by an orthodontic band around the incisors. Thirty-eight Wistar rats were used in two series of experiments with an observation period of 2 weeks. In each of the series there were one control and three experimental groups. Control rats were left untreated, while rats of the experimental groups wore either uninoculated or inoculated plates, or had their palatal mucosa smeared with the yeast. For cytologic examination the palate was scraped in Series I and the fitting side of the plate in Series II. After 1 week a generalized simple inflammation had developed in the palate of most animals of the experimental groups. It was most severe and persistent in rats with inoculated plates. Histologic signs of inflammation and hyphal formation were also most pronounced in this group. Hyphae did not invade the epithelium. Except for an initial loss of body weight, which was restored by day 10 or 12, the rats tolerated their plates. The Wistar rat seems to be well suited for experimental studies on denture stomatitis.


Abrasive wear of filled and unfilled resins in vitro.

The present investigation was initiated to evaluate the wear resistance of filled and unfilled resins which may be suitable for fissure sealing. Two commercial unfilled resins (Adaptic Glaze and Delton), three degrees of diluted composite (Adaptic diluted with Adaptic Bonding Agent) and undiluted composite (Adaptic) were studied. Abrasion was caused by 400-grit silicon carbide paper in a mechanical surface grinder. Ten specimens of each of the material samples were tested. The unfilled resins were  abraded three to six times more readily than the composite samples. Undiluted composite was the most resistant, whereas samples of increasing degrees of dilution were abraded to increasing extents. However, even the most diluted composite, although very fluid, was at least three times more resistant than the unfilled samples. The results of this study indicate that adding inorganic filler particles to a resin, even in small amounts, greatly enhances the wear resistance of such materials.


Reasons for replacement of amalgam dental restorations.

Recurrent caries appears to be the most frequently occurring reason for replacement of amalgam dental restorations. Recent developments of amalgam alloys have improved the clinical appearance of such fillings but the possible influence on their durability is uncertain.


Creep and beta1 formation in dental amalgam.

Measurements of creep, beta1 phase content and gamma1 lattice parameter were performed on four conventional amalgams stored at 37 degrees C up to 2 years. The creep values  and lattice parameters decreased and the beta1 content increased during this period; the most prominent alterations took place within the first 6 months.


Contamination of toothpaste and toothbrush by Streptococcus mutans.

Toothbrushes and toothpaste tubes used by persons infected with S. mutans were examined for the presence of this microorganism. Fifteen minutes after brushing greater than 10(6) S. mutans were isolated from the toothbrushes and after ordinary storage for 24 h 10(4) were recovered. From two out of 10 toothpaste tubes S. mutans was isolated from the orifice of the tube. The implications of these findings for the spread of the microorganism are discussed.


Effects of local anesthetics on the respiratory activity in vitro of cells in the dental pulp.

The respiratory activity of isolated dental pulps from rat incisors was studied using a Gilson respirometer. The activity was compared with activities after administration of varying concentrations of commercial standard solutions of lidocaine with and without adrenaline and prilocaine with felypressin. Above a 2.5% concentration of the standard solution added to the respiratory medium a significant inhibition was registered.


Pseudo-Chediak-Higashi anomaly in promyelocytic leukaemia associated with intravascular coagulation.

Giant granules were present in the cytoplasm of marrow granulocytes and in abnormal immature leucocytes circulating in the blood of a patient with promyelocytic leukaemia associated with intravascular coagulation. These granules resembled those seen in Chediak-Higashi syndrome.


Acute eosinophilo-myelomonocytic leukaemia, one of the 'in between leukaemias'.

A patient is reported to have an in between type of acute myeloid leukaemia, namely acute eosinophilomyelomonocytic leukaemia. The blasts in the peripheral blood showed a definite transition towards immature monocytes. The bone marrow contained 65% blasts and 13% eosinophil promyelocytes. The large number of immature eosinophils in the bone marrow strongly support the view that they were part of the leukaemic process and not merely a reactive eosinophilia.


Program evaluation as health services research.

Evaluation of health program is necessary for more rational allocation of scarce resources. Steps in evaluation include: 1)Specification of the program goals, 2) Description of the program, 3) Definition and measurement of outcome, 4) Assessment of intervening variables, 5) Analysis of data, and 6) Implementation of findings. Examples from impirical evaluation studies are presented to illustrate one practical application of these steps. While the random controlled trial is a most powerful tool, it is very difficult to apply in complex human services programs. In the future, more use of multivariate matching of groups to approximate a quasi experimental situation will be needed. Education of health professionals in methods of evaluation is necessary to promote planned improvement in health services.


Posterior uveitis and retinal dystrophy. A case report.

A 40-year-old patient with a bilateral posterior uveitis is presented and it is suggested that this may be a reflection of an underlying dystrophy.


Retinal detachment surgery. Buckling procedures and drainage of subretinal fluid.

The encircling procedure, while still an important part of succesful retinal detachment surgery, has been found to be unnecessary in a wide variety of retinal detachments which can be more simply treated by local buckles. A much more important aspect of the detachment procedure, however, is the avoidance where possible of drainage of subretinal fluid.


Management of diabetic retinopathy.

There is now evidence from clinical trials that patients with diabetic retinopathy treated by photocoagulation may have their visual life extended, so that they may lead socially, professionally, and economically independent lives. The establishment of treatment centres for photocoagulation is now urgently required. The exact indications and techniques are not clearly defined.


The Fyodorov intraocular lens.

The Russian-made Fyodorov intraocular lens has been inserted into one hundred eyes. The installation and the postoperative course has been no more difficult than that experienced with other types of lens, and it has not been necessary to remove any of them.


Effect of photocoagulation on the barrier function of the pigment epithelium. II. A study by electron microscopy.

The healing of retinal photocoagulation burns and their permeability to colloidal carbon and horseradish peroxidase was studied by electron microscopy. Recent burns showed disruption of intercellular junctions in the pigment epithelium. By 2 days there was infiltration of the burn by proliferating pigment epithelial cells, and by 7 days the burn was composed of a mass of interconnected cells of pigment epithelial and Müller cell origin. Fresh photocoagulation burns result in complete disruption of the blood-ocular barrier, permitting particles as large as colloidal carbon to pass between disrupted pigment epithelial cells. Healed photocoagulation burns remain permeable to smaller tracers such as horseradish peroxidase.


Role of pituitary vasopressin in the formation and dynamics of aqueous humour.

The role of pituitary vasopressin (antidiuretic hormone--ADH) in the formation and dynamics of aqueous humour was studied in rabbits employing different techniques. Using isolated ciliary body preparations the changes in transepithelial short-circuit current were measured, and natural vasopressin and Lys8-vasopressin were found to increase the transepithelial short-circuit current at concentrations less than 10 muU/ml (i.e. within the physiological range), indicating increased sodium transport across the ciliary epithelium. In another series of experiments with intact rabbits given an ethanol load to suppress endogenous ADH, administration of exogenous vasopressin raised the intraocular pressure, and a similar effect was observed when endogenous ADH production was stimulated with nicotine. Direct measurements of outflow showed that vasopressin was without effect when given intravenously and that the only effect when given intracamerally was to increase the facility which would tend to lower rather than raise the intraocular pressure. Finally, the intra-arterial and intravenous effects of vasopressin on circulation in the iris and on the intraocular and systemic arterial pressures were studied. Local effects on the vascular bed in the eye and changes in systemic blood pressure were observed only at rates of administration well in excess of the physiological range for endogenous vasopressin production. It is concluded that, at physiological levels, antidiuretic hormone can stimulate active sodium transport into the eye thereby tending to raise the intraocular pressure, and it is suggested that this may act as a homeostatic regulating mechanism limiting changes in the rate of formation of aqueous humour and in intraocular pressure which might otherwise result from diurnal variations in the state of body hydration. This also offers some explanation for the ocular hypotensive action of ethanol.


Hyperproduction glaucoma.

Hyperproduction glaucoma is a tonographic clinical entity characterized by high IOP and normal C: 1. It may occur as open angle glaucoma spontaneously or pharmacologically induced. 2. It may be an inflammatory ocular hypertension characterized by a breakdown of the blood-aqueous barrier. The treatment of choice is topical and/or systemic drugs such as epinephrine and acetazolamide which inhibit aqueous inflow; glucocorticoids should be added in cases of the inflammatory type. The pathogenesis is directly related to hyperproduction of aqueous, depending chiefly on increased capillary hydrostatic pressure at the level of the ciliary body.


[Osteosarcoma of the thyroid gland (author's transl)].

A case of an osteogenic sarcoma of the thyroid gland is reported. From the cases described in the literature the most important points of interest of these rare thyroid neoplasms are summarized and the histological differentialdiagnostic criteria to anaplastic carcinoma are discussed.


Percutaneous fine needle aspiration biopsy of the pancreas. Cytodiagnosis of pancreatic carcinoma.

Percutaneous fine needle aspiration biopsies were performed on thirty-four patients with clinical suspicion of pancreatic carcinoma. The biopsies were positive in twenty-four of twenty-six patients with proven pancreatic carcinoma and there were no false positive results in this series. The various cytologic appearances of different kinds of cellular components and cytologic features of the pancreatic carcinoma observed in the aspiration biopsy were presented and illustrated. The punctures and aspirations of these thirty-four biopsies did not reveal any immediate complication with one exception of exacerbation of pancreatitis. Percutaneous fine needle aspiration biopsy of the pancreas under the guidance of ERCP or angiography is a reliable diagnostic method for pancreatic carcinoma.


Cytologic diagnosis of bronchial mucoepidermoid carcinoma by fine needle aspiration biopsy.

The cytologic findings in percutaneous fine needle aspiration biopsy of two bronchial mucoepidermoid carcinomas are presented. The main features of this unusual tumor of the lung observed in the aspiration biopsy are sufficiently distinctive from those of other lung lesions to be diagnostic.


The effect of water soluble contrast material on urine cytology.

Urothelial cells were obtained from the pelvicalyceal system of ten patients by retrograde pyelography using a 60% solution of meglumine diatrizoate (Hypaque meglumine 60%, Winthrop Laboratories, New York, New York). The cells showed some cellular shrinkage, nuclear pyknosis, fragmentation, and occasionally cytoplasmic vacuolization. Cells from bladder urine obtained from 14 patients after an intravenous urogram using a 50% solution of sodium diatrizoate (Hypaque sodium 50%) showed no cytologic changes. In vitro exposure of bladder urothelial cells to 50%, 25% and 12.5% Hypaque sodium and 60%, 30% and 15% Hypaque meglumine as well as exposure of mesothelial cells to 50% Hypaque sodium resulted in the same cytologic changes as those seen when the urothelium was exposed in vivo to this material during retrograde pyelography. The mechanism for this effect, therefore, is by direct action of the contrast media on the cells and is concentration dependent. The cytopathologist should be aware of the changes induced by radiographic contrast materials such as Hypaque sodium or meglumine when interpreting urine cytology. This knowledge may be useful in preventing false positive diagnoses.


The numerical composition of cellular samples from the female reproductive tract. V. Cell cluster patterns in cases of invasive squamous carcinoma of uterine cervix.

One hundred fifty thousand cells in a vaginal, ectocervical and endocervical smears from 25 patients with poorly to moderately differentiated squamous cancer of the uterine cervix were evaluated to determine the numerical composition of cellular clusters, the sizes of the clusters and the cellular types contained. The study is a baseline assessment for the design of automated cytology devices for which monocellular layers or individual cells are of importance. The evaluation indicates that the majority of cells appear in actual or facultative clusters, and that dispersement or breaking up of these clusters can yield improved machine-readable samples.


Patient classification based on cytologic sample profiles. I. Basic measures for profile construction.

Past efforts in the field of automated cell recognition have focused upon the separation and classification of cell types. From these efforts, large data banks have been built and work in the field is now shifting towards the practical application of this information for clinical diagnoses. This paper presents the initial results of work on a system developed to undertake the reduction of the masses of data into diagnostically useful patient cytologic sample profiles.


The atypical fluorescent body of the interphase nuclei of buccal mucosal cells and its relationship to the Y chromosome.

Buccal mucosal cells from 30 males and 20 females were examined for fluorescent bodies in the interphase nuclei. Chromosomal analysis using peripheral blood, stained with quinacrine mustard (QM), was carried out on the 30 males. The calculated lengths of the Y chromosome were found to be related to the percentage of the total fluorescent bodies, both normal (F-body) and atypical (Fa-body), and also to the proportion of Fa-bodies present in the interphase nuclei. The long Y individuals had a higher percentage of fluorescent bodies and a higher proportion of Fa-bodies while the short Y individuals had a lower percentage of fluorescent bodies and a lower proportion of Fa-bodies.


Prognostic and therapeutic values of peritoneal cytology in gastric cancer.

Exfoliative cytology of peritoneal lavage was performed at the time of laparotomy for 458 patients with gastric cancer. Positive cytologic specimens were obtained from 28 per cent of all cases and from 16 per cent of patients with curative surgery. Peritoneal cytology has revealed itself to be a good predictor of prognosis and an indicator for adjuvant therapies. In the group treated with curative surgery, the five year survival rate was 86 per cent for patients with intact serosa and negative cytologic specimens, and 52 per cent for patients with involved serosa and negative cytology. None of the patients who had involved serosa and positive cytologic specimens survived more than five years. A slight benefit of adjuvant chemotherapy was observed in the patients who had curative surgery with negative cytology. Long term immunochemotherapy, including repeated intraperitoneal administration of mitomycin-C, improved the lifespan of patients with non-curative surgery.


Epithelial atypias associated with intrauterine contraceptive devices (IUD).

A total of 99 gynecologic smears with atypical cells from 74 patients wearing IUDs was reviewed. Endometrial and cervical tissues were examined. The cases were categorized into two groups, reparative and epithelial atypias. The latter category was further divided into three subgroups: squamous, columnar, and indeterminate type. The squamous atypia has classical features and is believed to be not significantly related to the IUD. Columnar atypia can strongly suggest cells shed from an adenocarcinoma. Indeterminate type of atypia is characterized by abnormal cells which can resemble cells shed from carcinoma in situ. Unlike the latter, the atypical cells from IUD are usually multinucleated, contain nucleoli, and are not associated with cells from severe cervical atypia. They are probably of endometrial origin.


Fluorescamine as a cytochemical detection reagent for mammary carcinoma cells.

Fluorescamine is a versatile reagent for the cytochemical demonstration of primary amino groups. Following pretreatment with formaldehyde, only certain "protected" amino groups will react with fluorescamine. In the present study we show that compounds containing such amino groups are abundant in mammary carcinoma cells but undetectable in normal mammary gland cells. Most cytologically benign breast lesions are devoid of cells containing these compounds. Although much work remains to be done, the fluorescamine technique, which is rapid and simple to perform, may offer some help in the distinction between normal and malignant cells of the mammary gland.


The identification of atypical reserve cells in smears of patients with premalignant and malignant changes in the squamous and glandular epithelium of the uterine cervix.

Atypical reserve cells can be recognized in cervical smears by their characteristic exfoliation pattern and the fragility of their cytoplasm. The frequency of cases with such cells in 30,000 cervical smears is given, as well as that in 440 cases of dysplasia, epidermoid carcinoma in situ and invasive carcinoma. The presence of atypical reserve cells was found to increase in frequency with the increasing severity of the lesions. The bipotential character of these cells is discussed in relation to the possible progression to carcinoma in situ of the adenomatous type in two of the 42 cases of pure atypical reserve cell hyperplasia, and the presence of these cells in the majority of the 43 cases of adenocarcinoma of the uterine cervix in the present series.


Application of electron microscopy in the definitive diagnosis of effusions.

Electron microscopy was performed on a series of body cavity fluid specimens to evaluate the usefulness of this technique in the diagnosis of effusions. During a six-month period, 42 specimens were examined, 19 benign and 23 malignant. In two cases, the electron microscopic findings were crucial to the diagnosis. In a third case, electron microscopy helped to confirm the diagnosis and avoid unnecessary surgery. In a number of other cases, ultrastructural evaluation demonstrated special cell characteristics not evident in routine light microscopic studies. Based on these findings, we conclude that electron microscopy has a definite place in the diagnosis of effusions and should be increasingly utilized on a selective basis in the evaluation of body cavity fluid specimens. In addition, electron microscopic studies on cells in effusions provide an excellent source of material for educational and research purposes.


Fine needle aspiration cytology of metastatic myxopapillary ependymoma.

The cytologic features of metastatic myxopapillary ependymoma were described. The cytologic material obtained from a metastatic subcutaneous nodule by the thin needle aspiration technique was examined by light and electron microscopy.


Cytologic appearances of pheochromocytoma and medullary thyroid carcinoma occurring within a family.

The cytologic and histochemical characteristics of the tumor cells from medullary thyroid carcinoma and pheochromocytoma are described. In cell samples obtained from medullary thyroid carcinomas, amyloid deposits were detected both intracellularly and extracellularly. Medullary thyroid carcinoma cells, as well as pheochromocytoma cells, showed positive for both argentaffin and argyrophil reactions. The diagnostic significance of intracellular amyloid deposits and of positive silver and chromaffin reactions of the intracytoplasmic granules may be stressed in establishing the cytologic diagnosis of medullary thyroid carcinoma and/or pheochromocytoma.


Endoscopic cytology of the esophagus, stomach and pancreas.

Cytology and biopsy under direct vision using a fiberscope as microscopic diagnostic procedures for carcinoma of the esophagus and stomach were described, and results of cytology were evaluated as compared with the results of biopsy. The diagnostic accuracy of brushing cytology under direct vision using a fiberscope was 97% in 116 cases of esophageal carcinoma and 78% both in 119 patients with carcinoma of the gastric cardia and in 63 patients with gastric carcinoma exclusive of the cardia. The diagnostic accuracy for cytology of pancreatic juice collected by cannulation in 36 patients with carcinoma of the pancreas was 56%: 79% for carcinoma of the head of the pancreas, but only 35% for carcinoma of the body and/or tail. For the esophagus and stomach, the diagnostic accuracy of direct vision biopsy has become almost as high as with cytology. As such biopsies are easily performed, esophagogastric cytology has become in our clinics a supplementary routine to the biopsy, and is done in a limited number of cases in which the biopsy failed or was not appropriate. However, cytology of the pancreas, in which biopsy is difficult, is important as the only microscopic diagnostic procedure. There, the development of newer instruments and the improvement of techniques of cell collecting are required, as is the development of expertise in the interpretation of cells from pancreatic carcinoma, in which the diagnostic accuracy is still relatively low.


Cytologic and microbiologic aspects of vaginal Torulopsis.

Twenty-five cases of vaginal Torulopsis in 7,331 patients were confirmed by cultures, auxonograms and zimograms. The cytologic and microbiologic characteristics are described in these cases. The authors feel that a nonmicrobiologic diagnosis of vaginal Torulopsis can be made with a small margin of error since its clinical characteristics constitute a rather well-defined entity.


Urinary cytology in inverted papilloma of the lower urinary tract.

Urinary cytology is reported in six cases of inverted papilloma of the bladder. Abnormal features were found in five of the six cases. These were essentially an increase in the number of single or small clusters of degenerate transitional cells. As their cytologic features fell within the range of normal but degenerate transitional epithelium, a diagnosis of transitional cell tumor was not possible in these cases. Histologic features suggest that the desquamating cells may be derived from the surface epithelium of the papilloma, the underlying cords and trabeculae, or from the adjacent transitional epithelium.


The cytologic manifestations of lymphomatoid granulomatosis in cerebrospinal fluid. A case report.

The cytologic manifestations of lymphomatoid granulomatosis in the cerebrospinal fluid observed in a 50 year old white male with midbrain and spinal cord involvement are presented. The cerebrospinal fluid, obtained by cisternal puncture, contained predominantly undifferentiated medium-sized cells, which were retrospectively interpreted as reticular cells, These cells were characterized by an atypical nucleus with one or more prominent nucleoli and clumped chromatin granules, abundant cytoplasm and frequent amoeboid protrusions of the cytoplasmic membrane. Small numbers of undifferentiated plasmacytoid cells, lymphocytes, neutrophils and erythrocytes were evident. A few mitoses were also observed. These cellular characteristics are correlated with histology of the parent lesion in the midbrain obtained at the postmortem examination.


Cytologic detection of malignant mesothelioma of the pericardium.

We have reported a very rare case, malignant mesothelioma of the pericardium, with the initial diagnosis made by cytology from the smears of the pericardial fluid. There were numerous large clusters in the smears, in which two types of cells were mixed: adenocarcinoma-like round cells and fibrosarcoma-like spindly cells. Some round cells very much resembled benign mesothelial cells. Central collagenous stalks and brush borders with long microvilli in the round cell aggregates were specific for carcinomatous mesothelioma and differentiated the round cells from adenocarcinoma cells. On the other hand, the spindly and pleomorphic tumor cells, present both singly and in clusters, implied malignancy. Therefore, a preliminary cytologic diagnosis of probable mixed malignant mesothelioma was made. Histopathologic and electron microscopic examinations confirmed the cytologic description that this tumor was a malignant mesothelioma.


Cytologic detection of malignant melanoma in urine. A case report.

This case illustrates the diagnosis of malignant melanoma from a sample of urine by cytologic examination without prior knowledge of cutaneous malignant melanoma and in the absence of chemical melanuria. The exfoliated cells were large, oval and solitary. They contained large, vesicular, sharply defined, often regularly contoured, eccentric nuclei with red macronucleoli. Their cytoplasm was cyanophilic with granular, brown-black pigment that histochemically was melanin. These composite characteristics, distinctive for malignant melanoma, eliminated other diagnostic possibilities. Surgical management was guided by an accurate cytologic diagnosis. The report emphasizes the clinical usefulness and criteria for accurate cytologic detection of metastatic malignant melanoma in urine.


Cytologic detection of recurrent vaginal melanoma.

A case of malignant melanoma of the vagina is described. Ultrastructural examination of tissue from the neoplasm showed a well-developed protein framework similar in appearance to that seen in the slowly progressive lentigo maligna form of malignant melanoma from the general body skin. The spectrum  of malignancy known for most other neoplasms, from slowly and locally recurrent to highly aggressive, seems to be found similarly in vaginal malignant melanoma. The patient was treated with local excisions and followed by cytologic examination; she is without known deep or systemic spread after more than three years.


Primary adenocarcinoma of the vagina. A case report with light and electron microscopic study.

A case of primary adenocarcinoma of the vagina in a 46 year old woman is reported. The light and electron microscopic studies are highly suggestive of a Müllerian origin. The ultrastructural features are discussed in relation to clear cell adenocarcinoma of the vagina, establishing the differences between them.


An unusual second primary tumor. Report of a case of atypical carcinoid of the lung.

This case report presents a rare occurrence of two primary tumors in the same patient: a transitional cell carcinoma of the bladder and an atypical carcinoid of the lung. The time interval between a linear density and tumor growth to 3 cm, determined by chest radiographs, was 9 months. The rapidly growing pulmonary nodule was suspected to be a metastasis from the primary transitional cell carcinoma. Fine-needle aspiration revealed malignant cells. Lobectomy and light and electron microscopy revealed an atypical carcinoid. The definition of "atypical" requires revision to indicate this tumor's true potential.


Chronic eosinophilic pneumonia. Diagnosis by fine needle aspiration.

A case of chronic eosinophilic pneumonia in which the diagnosis was established by fine needle aspiration of the pulmonary lesions is presented. The cytologic findings included the presence of an inflammatory exudate consisting of 40% eosinophils, 25% neutrophils and the remaining cells comprised of lymphocytes, histiocytes, atypical columnar cells and cells probably of pneumocyte type II origin. Fine needle aspiration of pulmonary infiltrates can be quite helpful in confirming the diagnosis of chronic eosinophilic pneumonia and in differentiating it from other similar conditions including malignant lymphomas, if the clinical and radiologic appearances are also taken into consideration.


Study of the peripheral blood leucocyte population by Fourier analysis of the cell volume histogram.

The Fourier transformation is proposed as a technique for the description of cell volume histogram data. In order to test the applicability of this transformation, peripheral blood leucocytes were studied as model populations and the technique assessed by determining the extent to which the Fourier coefficients could be applied as descriptors of various pathologic abnormalities of the differential leucocyte count. Histograms of leucocyte volume distribution were plotted for 87 cases with a variety of abnormalities of the peripheral blood leucocyte population. These histograms were submitted to Fourier analysis and the coefficients thus obtained were used as the basis of a multivariate classification system designed to distinguish certain predefined abnormalities of the differential count. The results obtained suggest that Fourier analysis may prove a valuable technique for the interpretation of cell volume histogram data, and its potential applications are discussed.


Monosomy 14, monosomy 22 and 13q--three chromosomal abnormalities observed in cells of two malignant mesotheliomas studied by banding techniques.

Two malignant mesotheliomas were studied by banding techniques. Both neoplasms had a hypodiploid stemline. Five and eight marker chromosomes, respectively, were included in the stemline karyotypes. Monosomy 14, monosomy 22 and a No. 13 with an interstitial long-arm deletion were deviations found in both neoplasms. In addition, closely located regions in No. 1 and No. 3 were involved in both tumors in the formation of other marker types with dissimilar morphology.


Chromosome one polymorphism in a girl with the Chediak-Higashi syndrome.

In a girl with the Chediak-Higashi syndrome, a remarkable polymorphism of chromosome number one was identified by G and C banding. The association of the polymorphisms of constitutive heterochromatin with abnormal phenotypes is discussed. It is suggested that further cytogenetic studies might be performed in humans and animals with this rare autosomal recessive disorder in order to confirm the present findings.


A study of the natural history of oral preleukoplakia.

A preleukoplakia, conceived as a precursor stage of leukoplakia, is grey or greyish-white area with indistinct borders. The paper describes 659 preleukoplakias detected among 20,358 individuals in two districts in India. In Bhavnagar district 99.5% of the preleukoplakias were among men. 23.5% of the hookli (clay pipe) smokers had preleukoplakias. In Ernakulam district 79.6% of the preleukoplakias were found among men and the mixed habit group of bidi smoking and tobacco chewing had the highest prevalence (6.1%). The preleukoplakias showed a marked tendency to regress; among the bidi smokers in Bhavnagar, 75% regressed. In two patients preleukoplakia turned into carcinoma.


Cytophotometric and cytogenetic analyses of mouse lymphomas.

Simultaneous cytophotometric and cytogenetic analyses of spontaneous mesenteric lymphomas from three female mice are reported. No statistically significant deviation from normal lymph node cells could be detected for any of the tumors with respect to nucleic acid content. However, all three tumors demonstrated trisomy as the major cytogenetic anomaly. Chromosome 7 or 8 was found to be the extra chromosome in two of the lymphomas while identification of chromosomes was not possible in the third.


Recurrent headaches in relation to temporomandibular joint pain-dysfunction.

Two groups of 80 patients, one referred because of mandibular dysfunction and one seeking dental treatment, completed questionnaires concerning recurrent headaches. The function of the masticatory system of all patients was afterwards examined clinically. The investigation showed that clenching of teeth was correlated to the severity of headache. The frequency and severity of headache varied also with the severity of mandibular dysfunction. Of the variables included in the dysfunction index, only masticatory musculature painful to palpation was found to have a distinct relationship to headaches. This implies that functional treatment is indicated in patients with headaches and tendernes of the masticatory musculature. Functional examination of the masticatory system should therefore be included in investigations of recurrent headaches.


Effect of methods of sample taking on the cytologic diagnosis of lung tumors.

By the evaluation of unselected cytologic specimens taken by different methods, the possible effect of sampling on the cytologic diagnosis was analyzed. A statistically significant tendency was established for certain tumors to be better diagnosed by certain methods of sampling. In order of the increasing radicality of the method, the following significant coincidences were found and discussed: Sputum for the diagnosis of bronchialalveolar cell carcinoma and epidermoid carcinoma (cornified cells); Bronchial washing for oatcell (undifferentiated) carcinoma and epidermoid carcinoma (moderately cornified cells); Bronchial brushing for epidermoid carcinoma (not cornified cells) and large cell (undifferentiated) carcinoma; Transthoracal needle aspiration for polymorphic carcinoma and metastasis; and Pleural effusion for the diagnosis of metastasis, bronchogenic adenocarcinoma and mesothelioma. If the clinical features indicate a certain tumor type, the data give evidence that it is advantageous to adjust the method of sample taking to the expected tumor type as far as possible. In the case of necessary re-examinations, transthoracic needle aspiration and bronchial brushing should especially be used, in addition to the self-evident examination of material produced spontaneously, i.e., sputum and pleural effusion, because of the relatively high expected value of these methods for positive diagnoses in re-examinations.


Powder data file as a tool for identification of dental gold alloys.

In order to obtain data for a prospective powder data file for dental gold alloys X-ray diffraction photograms of 75 different gold alloys were taken using a Guinier-Hägg camera and CuKalpha1 radiation. Before the X-ray photograms were taken the alloys had been heat treated at 700 degrees C, 800 degrees C or 900 degrees C depending on the solidus temperatures of the alloys. The lattice parameters of the samples were determined from the X-ray photograms. These data were supplemented by a specification of the interplanar spacings of the three strongest lines on the diffraction patterns and also by the relative intensities and Miller indices of these lines. An extensive library of reference films is to be collected and this and other details of the file are discussed. It is not intended that the file be used to identify a particular alloy but merely to identify the type of alloy. Thus it can serve as a guide to facilitate the proper selection of gold alloys within practical clinical dentistry.


A comparative cytologic study of 100 urine specimens processed by the slide centrifuge and membrane filter techniques.

An evaluation of cytology preparations made by the slide centrifuge and membrane filter techniques was made on 100 consecutive urine specimens from 46 patients. The membrane filter technique gave better cellular recovery and produced slides qualitatively superior to those prepared by the slide centrifuge technique. The former, therefore, is the recommended method. The slide centrifuge might be of greater value with urine of low cellularity where the membrane filter has provided only a few to practically no cells. The diagnostic accuracy on clinical follow-up of the patients included in this study is 94% for both methods, with no false positive cases and a 6% false negative rate. This high degree of accuracy indicates that these concentrating methods may lead to clinically valuable information if used routinely in the cytology laboratory.


Lactate dehydrogenase (LDH) isoenzyme pattern in normal and inflamed human dental pulp.

Enzyme variants may serve an adaptive role in providing the correct vectorial properties for the metabolism of a tissue, or broadening its environmental tolerance range. To determine if the LDH isoenzyme pattern of human dental pulp changes during inflammation, supernatants from normal and inflamed dental pulp homogenates were separated by polyacrylamide gel electrophoresis. Inflamed pulps had a higher M subunit content and a markedly increased enzyme activity. These results might reflect adaptive changes at the enzyme level associated with a partial shift towards anaerobic metabolism during inflammation of the pulp.


Computerized cephalometrics.

A method to analyse cephalometric radiographs with the help of a computer has been described. With the system a cephalometric radiograph can be analysed and the results compared to corresponding analyses of individuals with ideal occlusion. The results can also be visualized as schematic figures of the patients. The computer calculates means and standard deviations of groups of individuals and can visualize the results as mean figures. A program to train operators in pinpointing radiographic reference points has also been developed. A methodological study has shown that the errors of the computerized method are of little importance compared to the errors made in pinpointing radiographic reference points. A clinical test of the system has shown that inexperienced operators are able to use the system after about one hour's training. The method is about twenty minutes faster compared to an ordinary manual radiographic analysis. Some future aspects are also discussed.


Incisal overjet and traumatic injuries to upper permanent incisors. A retrospective study.

The relationships between traumatic injuries to upper incisors and incisal overjet were studied in a sample of 1445 orthodontically untreated children aged 7 to 16 years. Traumatic injuries of the hard dental tissues and exarticulations of teeth were recorded. The frequently of injuries was 14.2% in children with normal overjet (0--3 mm), 28.4% in children with increased overjet (3.1-6mm), and 38.6% in children with extreme overjet (greater than 6 mm). The severity of injuries was also greater in children with extreme overjet than in children with overjet ranging from 0 to 6 mm. Furthermore, the range of injuries increased in relation to the overjet. Two or more injured incisors were found in 19.2% of the children with normal overjet, in 22.2% of the children with increased overjet, and in 46.7% of those with extreme overjet.


Serial determination of serum ferritin in children with acute lymphoblastic leukemia. Evaluation of its usefulness as a prognostic index.

Thirty children with acute lymphoblastic leukemia were monitored with serial serum ferritin determinations for up to 17 months. In children with acute lymphoblastic leukemia before initiation of therapy, or in relapse, the mean serum ferritin concentration was 636 microgram/l. In children who went into primary remission. the mean serum ferritin concentration fell from 265 microgram/l prior to start of treatment, to 161 microgram/l after 3 months of treatment. Five patients relapsed. Their serum ferritin levels prior to the relapses ranged from 7 to 135 microgram/l. At the time of relapse a further increase in serum ferritin was found in only 2 of the children. Thus, whereas high serum ferritin levels may signal disease activity in acute lymphoblastic leukemia, a normal serum ferritin level does not exlude disease activity or impending relapse.


The clinical use of a craniofacial growth atlas.

A method of using normative growth data from An Atlas of Craniofacial Growth in planning orthodontic treatment and monitoring growth and response to therapy has been presented. Since the Atlas contains data on many cephalometric variables for both sexes from the ages of 6 to 16 years, the clinician can use any popular cephalometric diagnostic analysis for individualized diagnosis, treatment planning, and assessment of results. A practical example has been shown, applying the method to a young girl wearing a Frankel appliance during the first phase of treatment.


Comparative study between the SFS and LFS rotation as a possible morphogenic mechanism.

Cephalometric tracings of twenty-seven untreated adult Caucasians, selected on a clinical impression of reduced lower facial height were studied and compared with tracings of nine untreated adult Caucasians with increased lower facial height. Various linear and angular measurements for both groups were analyzed and compared. As has been previously described for the SFS, the LFS group can be subdivided into at least two subtypes. Subtype I is characterized by a long ramus, increased OP-PP, an increased SN:MP-angle, and excessive lower anterior facial height. These persons manifest the most typical clinical characteristics of the long face syndrome and have excessively long faces. In Subtype II, however, extreme backward and downward rotation of the mandible, combined with a short or extremely short ramus, is associated with an increase in lower anterior facial height. The increase in posterior maxillary height was not striking in this subtype. It was demonstrated that many of the characteristics of the LFS group and the SFS group can be explained by clockwise or counterclockwise rotation of the mandible "in concert" with the hyoid, tongue, pharynx, and cervical spine. The LFS group was characterized by a clockwise rotation of the mandible "in concert" with the hyoid, tongue, pharynx, and cervical spine. The mandible of the SFS group rotated similarly, but in the opposite counterclockwise direction. The vital need to maintain patency of the upper airway at the level of the base of the tongue may account for rotation in the LFS.


A cephalometric evaluation of high-pull molar headgear and face-bow neck strap therapy.

The effects of two different extraoral appliances were evaluated over a 1-year period. Of the thirty-seven cases selected for study, twenty were treated with a face-bow neck strap and seventeen were treated with a high-pull molar headgear. Patients ranged in age from 10.10 to 16.6 years and averaged 13.4 years. The appliances exerted less than 600 Gm. of force per side and were worn for 12 to 16 hours per day. All cases were fully banded, and extraction and nonextraction treatment were included. An analysis of pretreatment data revealed a high degree of selection. Patients selected for high-pull treatment generally exhibited larger anterior face heights, steeper mandibular plane angles, and a greater amount of tooth eruption of the upper first molars than the patients selected for neck strap therapy. A control group of ten untreated subjects was matched to each treatment group to permit assessment of the impact of treatment on growth. Relative to normal growth, treatment with face-bow neck strap traction tended to direct the maxilla and mandible downward and backward. The palatal plane was lowered anteriorly and point A was retracted. The maxillary molars were extruded, and concomitantly an increase in anterior face height and mandibular plane angle was observed. On the other hand, the high-pull molar headgear traction resulted only in increased mandibular molar eruption. However, there was also a nonsignificant tendency for point A to be held back and for lower anterior face height to increase. The comparison of the two treatment samples revealed that the functional occlusal plane was tipped down at the back as the maxillary molars were more extruded in the neck strap sample. In the high-pull sample, the functional occlusal plane was unchanged and the mandibular molars were more extruded than they were in the neck strap group.


Cephalometric analysis: comparisons between maximum intercuspation and centric relation.

Cephalometric analyses were made of forty-six preorthodontic patients with the mandibular teeth in maximum intercuspation. A Centric-Ceph was used and the analysis was repeated on these patients with the mandible in centric relation. The following conclusions are drawn: 1. No clinically useful prediction may be made from cephalometric radiographs concerning the amount of mandibular deflection from centric relation to maximum intercuspation of teeth. 2. There are differences in cephalometric measurements with the mandible in the two different positions. However, with the exception of a few cases, the differences are slight. 3. The individual cases that have the largest discrepancies tend to be Class II malocclusion cases. The orthodontist should be aware of those cases and be prepared to articulate them for diagnosis.


Onset of chronic illness in children and adolescents: psychotherapeutic and consultative intervention.

Patients with disparate chronic illnesses exhibit specific and similar reactions during the onset of disease. This paper suggests the importance of psychological intervention during the initial stages of disease, and makes recommendations for psychotherapeutic and consultative management of the patient. Consideration is given to the effects of such intervention on both patient and psychotherapist.


A detailed consideration of the line of occlusion.

With the re-evaluation of the concept of the "line of occlusion" the author proposes a contemporary definition: "a distinctively individual line at the incisobuccal contact, with a location, position, and form to which the teeth must conform to be in normal occlusion." In other words, it is that position which the teeth must occupy to be in stability and harmony with each other and with all other anatomic structures.


A comparison of lower face changes.

A comparison has been made of cases treated with deliberate anchorage preparation (Class III elastic mechanics) prior to Class II elastic mechanics and cases treated with Class II elastic mechanics only. Treatment goals were the same. Extraoral traction was used as necessary to achieve those goals. Appliance quantity and size were the same. Age, sex, and severity of malocclusion were comparable. It remained that the anchorage-prepared cases demonstrated a flattening of the mandibular plane, greater retraction of the lower incisors, more retraction of midface, and more protraction of lower face. These changes of lower face are consistent with the normal growth changes of the human face.


Aerosol bronchodilator administration in ventilator circuits.

A device is described enabling bronchodilator drugs in aerosol form to be added to the inspired gas of ventilated patients using standard metered-dose containers.


The use of the Cape Minor ventilator with the circle absorber system.

Various methods by which the Cape Minor ventilator may be used in the circle absorber system are described and the problems of the circuits described are considered. A new valve system is described which overcomes most of the problems encountered with other circuits. The economic advantages of an inexpensive ventilator and absorber system which is economical are obvious.


Percutaneous cervical cordotomy and the injection of the pituitary with alcohol.

The two techniques of percutaneous cordotomy and pituitary injection of alcohol have been considered as methods applicable to the relief of intractable pain in inoperatable cancer. Percutaneous cordotomy is best indicated for unilateral pain below the C5 dermatome and will produce absolute relief of pain until death in up to 90% of patients. The injection of alcohol into the pituitary gives some relief of bilateral pain in about 70% of patients. Twenty percent obtain complete freedom from pain for up to 4 months, and 20% have complete freedom from pain for over 4 months. Both methods require the use of an image intensifier X-ray machine and care in observing safety precautions is necessary for both techniques. The pituitary injection of alcohol is the simpler of the two techniques. It can relieve bilateral cancer pain and it may be used to relieve pain in the head and neck of cancer sufferers which cannot be relieved by other methods.


The mandibular dental arch: part III. Buccal expansion.

The tendency toward relapse in intercuspid width has been examined with those cases having final intercuspid width less than 27 mm showing significantly less relapse than those cases with final intercuspid width of 28 mm or more. The point of contact between the cuspid and first premolar has been introduced as a key point on the arch, determining arch width. An individualized norm has been derived for this measurement as a function of the patient's tooth size, facial pattern, and other variables based upon stable normal occlusions in treated cases. Those cases expanded to a dimension exceeding the norm by more than 1 mm showed a greater propensity toward relapse. The group following the norm was significantly more stable than the over- and underexpansion groups at the .025 significance level. An individual norm for intermolar width based upon the patient's facial pattern (using frontal and lateral X-rays) has been established. Cases showing relapse showed considerably less space between the lower molar and the JAG plane, and greater lower face height than stable cases. The results show that the space available for the permanent dentition can be estimated in advance of treatment based on the patient's own skeletal measurements, thus minimizing unnecessary extractions, relapse, and extended treatment time due to errors in diagnosis.


Simultaneous metastatic epidermoid carcinoma and chronic granulocytic leukemia.

The second reported patient with simultaneous metastatic epidermoid carcinoma and chronic myelogenous leukemia is described. The difficulty of differentiating the leukemia from a leukemoid reaction is discussed. The incidence of, and importance of looking for, second primary cancers in patients known to have cancer is emphasized.


Growth and hormonal status of children treated for acute lymphoblastic leukaemia.

Growth and hypothalamic-pituitary function have been studied in children in long-term remission from acute lymphoblastic leukaemia (ALL). All 14 children are growing and developing normally; in the 8 children in whom the hypothalamic pituitary axis was investigated endocrine function is normal. Continuing long-term review of these children is essential, but hypothalamic-pituitary investigation is required only when there is a decrease in growth velocity or delay in the onset of puberty.


Decreased platelet monoamine oxidase activity in chronic schizophrenia, shown with novel substrates.

Platelet monoamine oxidase (MAO) was kinetically evaluated in chronic schizophrenics and matched controls, using substrates of major physiologic importance and substrates of particular interest in the study of schizophrenia, such as serotonin (5-ht), N,N-dimethyltryptamine (DMT), 5-methoxytryptamine (5-MT), and dopamine (DA). Substrates were measured at six concentrations; values for maximal velocity (Vmax) and Michaelis constant (Km) were obtained by using Lineweaver-Burk plots. The Vmax was decreased for all substrates in chronic schizophrenia and the Km was decreased for DA, 5-HT, and DMT, but remained unchanged for 5-MT. The value of Km/Vmax was similar for schizophrencis and normal persons when DA, 5-HT, and DMT were used as substrates, which may indicate that "uncompetitive" inhibition is responsible for the observed decrease in activity among chronic schizophrenics. The finding of a decreased Vmax but unchanged Km with 5-MT would be consistent with noncompetitive inhibition.


Pathogenesis of some traumatic and degenerative disorders of soft tissue.

1. Trauma to soft tissues is associated with tissue rupture and formation of a haematoma. 2. Such lesions cannot be expected to resolve without complications in the absence of appropriate treatment. 3. Myositis ossificans should be anticipated in lesions of muscles adjacent to their attachments. 4. Chronic tendonitis and enthesopathies not associated with inflammatory connective tissue disease are primarily degenerative in nature and are probably the result of comparative ischaemia. Mesenchymoid transformation is a feature of both.


The onset of anorexia nervosa.

In a retrospective study of case notes a number of experiential and psychological factors were discerned of possible importance to the psychogenesis of anorexia nervosa. These factors included issues of dependence and independence, sexual challenge, concern about obesity, and a variety of other, less specific stresses. Attempts to confirm the findings by means of a prospective study were impeded by difficulties in defining the onset of the illness. While in some patients the occurrence of anorexic type behaviour led immediately to weight loss, in others there was a significant delay between the onset of behavioural change and consequent emaciation.


Time-limited psychotherapy with university students.

A report of the developments that stemmed from initial observations in time-limited psychotherapy with University student patients. These led to the conceptualization of the process not in terms of focal conflict but in the development of the relationship itself. Autonomy came to be seen as the central issue and a similarity was noted between the pattern of the relationship in therapy and a proposed developmental model of the growth of autonomy. Within this framework reciprocity became the focus of the relationship dimension. A case illustration is given the significance of time and separation discussed and some therapeutic and theoretical implications considered.


Suicide attempts 1973-1977--urban Hobart. A further five year follow up reporting a decline.

In urban Hobart 1968--1972 there was a steep climb in the self-poisoning rates, reaching a peak in 1972. This rise was largely attributed to an increase in self-poisonings by young males and an overall increase by females. During this period psychotropic overdoses commenced. In 1973--1977 self-poisoning and self-injury rates were calculated. Contrary to the previous five year experience rates either declined during 1973 to 1977 or fell from a previous high rate to level off at a lower rate. These changes have particularly affected both sexes under 35 years. The abuse of psychotropic substances continued to soar. A possible explanation of these changes may lie in local factors. The results are contrasted with the most recent literature.


Australasian suicidology.

A survey has been made of 38 studies of Australasian medical and hospital experiences with attempted and completed suicide in the last decade. The range and depth of the data recorded in the 38 studies have been analysed and reported together with comments on some other multivariate studies from overseas. The implications of these studies for the current medical views about attempted and completed suicide (are they two distinct types or do they lie on a continuum?) are discussed within a framework relating to the views about suicide that are embedded in our culture.


Adjustment of adopted children and demographic profile of adoptive parents.

Using 51 manifestations of childhood dysfunction as index of adjustment, the relationship was investigated of 9 demographic characteristics of applicants for adoption to the adjustment of children, when aged 7 to 7 1/2 years, whom they had adopted. 'Good adjustment' was associated with the father having at least completed high school, having a non-manual occupation and an above median income. There was a trend for it to be also associated with the mother having at least completed high school, being under 30 years of age, and with the couple's non-committal on (i.e., not outright rejection of) the possibility of adopting a hard to place child. The findings were discussed critically and it was concluded that the study demonstrated the feasibility of establishing objective empirical criteria for the selection of adoption applicants.


Significance of genetic factors in Gilles de la Tourette syndrome: a review.

Observations suggesting a genetic basis for Gilles de la Tourette syndrome are reviewed with particular emphasis on the finding of familial aggregation. Studies of both Tourette syndrome and simple tic have found that approximately 30% of patients have a positive family history of tic. The significance of this figure depends on a number of factors, in particular the prevalence of positive tic histories in the population. If the latter figure is 10%, which the best available evidence suggests is a reasonable estimate, approximately 30% of families in the general population would be expected to contain at least one present or former tiquer. It is argued, therefore, that the family aggregation findings in Tourette syndrome do not support the hypothesis that the condition has a significant genetic component. Methodological considerations for future research are discussed.


Surface antigens in acute myeloblastic leukaemia: a study using heterologous antisera.

This study analyses the activity of 95 antisera raised in rabbits against human leukaemic myeloblasts. A number of different means were used to immunize both normal rabbits and rabbits which had been treated to render them tolerant of normal human splenic leucocytes. Different immunization schedules included the use of different doses of untreated myeloblasts, as well as myeloblasts treated with neuraminidase, antibody against human spleen cells or glutaraldehyde. Analysis of the sera was carried out using two sensitive techniques for detecting cell surface antigens: a radioactive anti-immunoglobulin binding assay using 125I-horse-F(ab')2-anti-rabbit-Fab and a K-cell-mediated cytotoxicity test using rat spleen cells as effectors. (i) The unabsorbed sera showed similar mean titres against leukaemic myeloblasts and normal splenocytes. (ii) Extensive absorption with pooled cadaveric spleen were required to remove antibody against polymorphic antigens. (iii) 17/95 antisera had activity against at least some leukaemic myeloblasts after extensive absorption with cadaveric spleen. (iv) Some of the 17 absorbed sera with selective activity for myeloblasts also reacted against PHA-induced lymphoblasts. (v) Although the 17 absorbed sera showed little or no activity against marrow in the above assays normal human marrow totally absorbed all residual activity in these sera against leukaemic myeloblasts. We conclude that although these sera contain activity against antigens common to leukaemic myeloblasts and a minority population of normal marrow cells, they have no detectable activity against leukaemic-specific antigens.


Platelet storage pool deficiency and prostaglandin synthesis in chronic granulocytic leukaemia.

Platelet function was evaluated in eight patients with chronic granulocytic leukaemia (CGL), seven Ph1 positive and one Ph1 negative. Seven of the eight patients' platelets had an absence of the second wave of adrenaline induced aggregation on at least one occasion, while five had impaired collagen aggregation. The platelets of all seven patients with abnormal responses to adrenaline, aggregated with arachidonic acid, thus ruling out a cyclo-oxygenase deficiency. A marked decrease in the ADP, serotonin, and dense body content of platelets was found in all five patients evaluated. Mixtures of CGL patient platelets with platelets from normal donors who had ingested aspirin gave a normal biphasic response to adrenaline. Normal release of the storage pool contents from aspirin treated platelets was shown by stirring a mixture of CGL platelets and 14C-serotonin labelled aspirin treated platelets with adrenaline. The CGL platelets alone or in the mixture produced malondialdehyde in response to adrenaline. These experimental results suggest that CGL platelets have a storage pool deficiency but can synthesize prostaglandins and thromboxanes in response to arachidonic acid and adrenaline.


Low-dose heparin in the management of acute myelocytic leukaemia.

The effectiveness of heparin in acute leukaemia complicated by disseminated intravascular coagulation (DIC) is still controversial. In this regard low-dose heparin was found to be therapeutically effective in three patients suffering from acute myelocytic leukaemia and DIC. With respect to the contraindication of high-dose heparin in these conditions the low dose regimen appears to be a valuable alternative.


Growth of human acute myeloblastic leukemic (aml) cells in vitro.

Human normal and granulocytic leukemic progenitor cells produce colonies and clusters when cultured in the plasma clot system in vitro. The number of colonies formed and their cellular composition was comparable to that reported for the system (soft agar) usually used for these studies. The plasma clot system has the advantage of permitting in situ morphologic and cytochemical characterizaion of the cells within the colonies. A comparison was also made of the growth of leukemic cells in suspension cultures placed within conventional and modified Marbrook flasks. These studies demonstrated no advantage for the Marbrook system in cultures incubated for up to 7 days.


[Practical aspects of mutagenesis--today and later].

The struggle of medicine and biology with the phenomenon mutation is discussed from a philosophical (C. BRESCH: "Zwischenstufe Leben") and a practical point of view. In the beginning age of the intellectual evolution mutations have become a pruely negative relict of the declining phase of the biological evolution. Effective means of medical genetics suitable in reducing the number of manifest mutants are: prenatal genetic diagnoses in as many pregnancies as possible; protection from exogenous mutagenic sources such as chemical mutagens and consequent protection from ionizing radiations, especially in the field of medicine. Possibly also effective is conventional genetic counselling which, however, should be supported for purely humanitarian reasons. The author denies the necessity for extraordinary efforts in the field of "genetic engineering" for various reasons, without casting doubt on the purely scientific aspects of molecular genetics.


Genetic engineering.

The field of genetic engineering is reviewed with a special emphasis on in vitro DNA recombinant technology. The basic principles of the biochemistry of DNA splicing and of gene transfer are described. An important distinction is made between the insertion and cloning of genes derived from genomic DNA ("natural" genes) and of DNA synthetized in vitro ("synthetic" genes). Cloning of genes synthetized from mRNA has provided the probes necessary for the identification of genomic clones, and recently it has made possible the synthesis of specific mammalian proteins in bacteria.


Prognostic implications of chromosomal findings in acute lymphoblastic leukaemia at diagnosis.

Chromosomes were studied on diagnostic bone-marrow samples from 39 children with acute lymphoblastic leukaemia (ALL). The patients were classified, according to the chromosomal characteristics of the major proportion of their leukaemia cells, into five categories; hyperdiploid, pseudodiploid, diploid, hypodiploid, and mixed. Patients in the hyperdiploid category had significantly longer first remissions than those in all other categories, and those in the pseudodiploid category had the shortest. Neither the absence of any normal cells nor the presence of detectable clones appeared to be an adverse feature. We suggest that the proportion of hyperdiploid cells, determined by conventional chromosomal staining techniques, may be used as an additional prognostic feature in childhood ALL.


Different cell clones in bone marrow and spleen of a patient with chronic myelocytic leukemia (CML) in blastic phase.

The chromosome study of a patient with chronic myelocytic leukemia in blastic phase revealed a 46,XY,Ph1/47,XY,Ph1,+8 cytogenetic constitution in bone marrow cells and a 46,XY,Ph1/48,XY,2Ph1,+19 cytogenetic constitution in spleen cells. As the cell clones exhibiting chromosome abnormalities in addition to the Ph1 chromosome evolved apparently independently, it is suggested that the acute transformation had a bifocal, myeloid and splenic origin.


Peripheral amino acid levels in patients with cancer.

Peripheral arterial and venous whole blood amino acid concentrations were determined in four groups of subjects after an overnight fast 1) normal people, 2) patients with cancer who had not lost body weight, 3) subjects with cancer who had lost more than 20% of body weight and 4) patients who had lost more than 20% of body weight from diminished intake due to cause other than cancer. Comparison of the arterial blood levels in the four groups showed that patients with cancer and weight loss had amino acid patterns different from patients who were malnourished for other reasons. Branched chain amino acids were normal in patients with malignant disease. Some gluconeogenic amino acids were reduced as in other subjects with weight loss but the characteristic rise in glycine seen with malnutrition was not present. Arterio venous differences in whole blood across the forearm showed no evidence of increase in venous excess in patients with progressive malignant disease, indicating no excessive protein catabolism in muscle tissue. The data are consistent with increased gluconeogenesis in malnourished cancer subjects, probably due to intrinsic change in hepatic metabolism.


Antitumor activity of interferon against murine osteogenic sarcoma in vitro and in vivo.

Murine interferon inhibited the growth of a continuous line of osteogenic sarcoma cells in tissue culture. Inhibition of tumor cell growth was documented by decreased clone formation in liquid medium, decreased tumor cell counts in monolayer cultures, suppression of colony formation in semi-solid agar, and decreased uptake of 3H-thymidine by the osteogenic sarcoma cells in culture. The capacity of anti-interferon antibody to block the tumor cell growth inhibitory activity of the interferon preparation suggested that interferon itself is the biologically active component of the interferon preparations. In vivo, a 7-day course of 30,000-60,000 units/day of type I interferon prepared in cell cultures either completely inhibited or delayed the appearance of tumors in experimental animals inoculated with osteogenic sarcoma cells by the sc route. The therapeutic efficacy of a preparation of murine sera containing type II interferon as well as other lymphokine activity was compared with the type I interferon preparation. Animals treated with 600 units of type II interferon were protected against tumor development as effectively as with 60,000 units/day of type I.


Activation of reticuloendothelial system macrophages and enhancement of host resistance to a transplantable osteogenic sarcoma in mice by an extract of Brucella abortus.

An aqueous-ether extract of Brucella abortus, Bru-Pel, enhanced resistance of mice to a transplantable osteogenic sarcoma (OGS). The results presented in this report suggest that Bru-Pel is an effective immunomodulator and that one mechanism through which it enhances host resistance is activation of phagocytic cells of the reticuloendothelial system. Peritoneal macrophages from mice inoculated with Bru-Pel 14 days previously were cytotoxic for OGS cells in vitro, limited the multiplication of vaccinia virus in cell cultures, and demonstrated increased chemiluminescence during phagocytosis. Furthermore, Bru-Pel enhanced host resistance to Listeria monocytogenes, in addition to viral infections and a transplantable tumor. These results support the hypothesis that Bru-Pel shares a number of characteristics with other recognized immunomodulating agents and suggest that further studies are warranted to better define the potential of Bru-Pel for immunotherapeutic regimens in man.


Activation of teratocarcinoma-derived hemoglobin genes in teratocarcinoma-Friend cell hybrids.

Hybrid cells formed by the fusion of murine teratocarcinoma and Friend erythroleukemia cells synthesize hemoglobin in the presence of chemical inducers such as dimethylsulfoxide (DMSO). By making use of the fact that the parental teratocarcinoma and Friend cells carried different alleles at the locus coding for the alpha chain of hemoglobin, it was possible to demonstrate that the teratocarcinoma-derived genes for the globin alpha chains are genetically active in hemoglobin-synthesizing hybrid cells. In addition, evidence is presented suggesting that the teratocarcinoma-derived genes for the beta-globin chains may also be expressed in the hybrids. Apparently the teratocarcinoma-derived genome has become reprogrammed to express erythroid functions following fusion of the teratocarcinoma cell to the Friend cell.


Heart disease screening of school children using abbreviated electrocardiogram and phonocardiogram.

By recording the electrocardiogram and phonocardiogram simultaneously using three abbreviated leads, 23,216 (6 and 7 years of age) elementary school children were screened for heart diseases. One hundred and one cases (0.44%) with congenital heart diseases, ten cases (0.04%) with acquired heart diseases and 100 cases (0.43%) with electrocardiographic abnormalities were discovered. From these observations, we conclude that this screening method applied on school children is useful in discovering the previously unrecognized heart diseases, especially atrial septal defect, primary myocardial disease and arrhythmia.


Studies on medically important flies in Thailand. V. On 32 species belonging to the subfamilies Muscinae and Stomoxyinae including the taxonomic keys (Diptera: Muscidae).

Flies from different geographical parts of Thailand were collected to study their medical importance as well as making a zoogeographical study. After examination, 32 species of 10 genera, Orthellia, Mitroplatia, Morellia, Pyrellia, Rypellia, Haematobia, Haematobosca, Stygeromyia, Haematostoma and Stomoxys, belonging to Muscidae were identified and these are reported in this paper. Some of these are newly recorded ones from this country. This study is based on the specimens available in the authors' collection.


Action of parathyroid hormone, with special reference to its anabolic effect on different kinds of tissues in rats (I).

The effect of parathyroid hormone (PTH) on the spongiosa of the proximal tibia and the alveolar bone in immature rats was studied using a time marker by the injection of lead acetate. 1) When intact rats were fed on a low calcium diet, the promotion of the apposition of the alveolar bone and the longitudinal formation as well as the resorption of the spongiosa were observed, but no change was detected in the serum calcium level. The resorption was more remarkable than the formation. 2) By parathyroidectomy (PTX) or thyroparathyroidectomy (TPTX) the effect described in 1) disappeared completely, but there subsequently occurred a fall of the serum calcium level and a marked inhibition of the formation and resorption. The decrease in the appositional formation was stronger than in the longitudinal formation. 3) When PTH was injected into the rats having undergone PTX or TPTX, all of the effects in 2) were reversed with the recovery of the apposition being at an extremely high rate. All the foregoing results indicate that PTH has an evident anabolic action in addition to the action of increasing the bone resorption and that the sensitivity to PTH is stronger in the periosteal bone than in the spongiosa.