text: user2766: Is there any tool available to predict the melting temperature for rna/lna oligos with its perfect complement ? 
  I know this amazing website from qiagen/geneglobe that estimates the tm value. 
  But is there any command line tool that does this? 
  I can estimate the normal tm value with RNAcofold and the -T option but there is no lna option. 
  I searched for a while but i can only find online services. 
  
  user6518: There are several programs for simulating/predicting DNA melting but you could try them for RNA.
  You can look here http://bioinformatics.org/meltsim/wiki/Main/HomePage.
  
  user3541: Both Bio.SeqUtils.MeltingTemp of Biopython and Primer3 offers different methods/algorithms to calculate the Tm value.
  
original_text: 2766: <p>Is there any tool available to predict the melting temperature for rna/lna oligos with its perfect complement ? </p>
  
  <p>I know this amazing website from qiagen/geneglobe that estimates the tm value. </p>
  
  <p>But is there any command line tool that does this? </p>
  
  <p>I can estimate the normal tm value with RNAcofold and the -T option but there is no lna option. </p>
  
  <p>I searched for a while but i can only find online services. </p>
   6518: <p>There are several programs for simulating/predicting DNA melting but you could try them for RNA.
  You can look here <a href="http://bioinformatics.org/meltsim/wiki/Main/HomePage" rel="nofollow noreferrer">http://bioinformatics.org/meltsim/wiki/Main/HomePage</a>.</p>
   3541: <p>Both <a href="https://biopython.org/docs/1.75/api/Bio.SeqUtils.MeltingTemp.html" rel="nofollow noreferrer">Bio.SeqUtils.MeltingTemp</a> of Biopython and <a href="https://primer3.org/" rel="nofollow noreferrer">Primer3</a> offers different methods/algorithms to calculate the Tm value.</p>
  