text: user8994: I am trying to build an index on kallisto on mac using R studio and have saved my FASTA file to my desktop, however, when I run kallisto index -i DI_mRNA.index DI_mRNA.fa.gz, there is an error saying Error: FASTA file not found DI_mRNA.fa.gz
  
  user77: As mentioned in a comment, your working directory lacks the fasta file.
  
original_text: 8994: <p>I am trying to build an index on kallisto on mac using R studio and have saved my FASTA file to my desktop, however, when I run <code>kallisto index -i DI_mRNA.index DI_mRNA.fa.gz</code>, there is an error saying <code>Error: FASTA file not found DI_mRNA.fa.gz</code></p>
   77: <p>As mentioned in a comment, your working directory lacks the fasta file.</p>
  