text: user12126: Is there any way that I can read SCF file in python like in R using sangerseqR, I have tried with Biopython, it seems it does not support this format.
  
  user12126: It was done using sangerseqR with rpy2 in python
      import rpy2.robjects as r
      from rpy2.robjects.packages import importr
      utils = importr('utils')
      utils.install_packages('sangerseqR', repos="https://git.bioconductor.org/packages/sangerseqR")
      utils.chooseBioCmirror(ind=1) # select the first mirror in the list
      utils.install_packages('sangerseqR')
  
  
      sangerseqR = importr('sangerseqR')
      Trace = sangerseqR.readsangerseq( file="1I1_F_P1815443_047.scf") #scf sequence file is now loaded
  
  sangerseqR Documentation(pdf)
  
original_text: 12126: <p>Is there any way that I can read <a href="http://staden.sourceforge.net/manual/formats_unix_2.html" rel="nofollow noreferrer">SCF</a> file in python like in R using <a href="https://rdrr.io/bioc/sangerseqR/man/read.scf.html" rel="nofollow noreferrer">sangerseqR</a>, I have tried with <a href="https://biopython.org/wiki/SeqIO" rel="nofollow noreferrer">Biopython</a>, it seems it does not support this format.</p>
   12126: <p>It was done using <a href="https://www.bioconductor.org/packages/release/bioc/html/sangerseqR.html" rel="nofollow noreferrer">sangerseqR</a> with <a href="https://rpy2.github.io/doc/latest/html/overview.html#" rel="nofollow noreferrer">rpy2</a> in python</p>
  <pre><code>    import rpy2.robjects as r
      from rpy2.robjects.packages import importr
      utils = importr('utils')
      utils.install_packages('sangerseqR', repos=&quot;https://git.bioconductor.org/packages/sangerseqR&quot;)
      utils.chooseBioCmirror(ind=1) # select the first mirror in the list
      utils.install_packages('sangerseqR')
  
  
      sangerseqR = importr('sangerseqR')
      Trace = sangerseqR.readsangerseq( file=&quot;1I1_F_P1815443_047.scf&quot;) #scf sequence file is now loaded
  </code></pre>
  <p><a href="https://www.bioconductor.org/packages/release/bioc/vignettes/sangerseqR/inst/doc/sangerseq_walkthrough.pdf" rel="nofollow noreferrer">sangerseqR Documentation</a>(pdf)</p>
  