text: user4127: I want to download the next generation RNA sequencing data in different cell lines. I want the data (alongwith normal) for p53 wild type, p53 knock-out and p53 null cell lines (human colon cancer). From where do I download this data? In fastq format if possible.
  Currently, I am going through SRA, to look for the datasets, using their search engine. But the process is extremely tedious. 
  Also can someone please hint about how to download the data from SRA, in case that's the only way to get data. I'm getting very confused. 
  
  user4121: I agree with Kamil on the downloading method.
  Moreover, SRA will give you your file in the raw format which is usually a Binary Alignment Map (BAM) or Sequence Alignment Map (SAM) 
  Download SRA toolkit available online, to convert your raw reads to fastq format. Follow the steps using command line. It is a very simple command to convert your SRA file to fastq which uses the fastq-dump command.
  Further reading: https://www.ncbi.nlm.nih.gov/books/NBK158900/
  Hope it helps! 
  
  user1135: I suggest you to try difference resources, including ArrayExpress. 
  A simple search at ArrayExpress returns multiple experiments which might be of your interest. Just a pointer, ArrayExpress is also synced with GEO.
  
original_text: 4127: <p>I want to download the next generation RNA sequencing data in different cell lines. I want the data (alongwith normal) for p53 wild type, p53 knock-out and p53 null cell lines (human colon cancer). From where do I download this data? In fastq format if possible.</p>
  
  <p>Currently, I am going through SRA, to look for the datasets, using their search engine. But the process is extremely tedious. </p>
  
  <p>Also can someone please hint about how to download the data from SRA, in case that's the only way to get data. I'm getting very confused. </p>
   4121: <p>I agree with Kamil on the downloading method.
  Moreover, SRA will give you your file in the raw format which is usually a <strong>Binary Alignment Map (BAM)</strong> or <strong>Sequence Alignment Map (SAM)</strong> 
  Download <a href="https://www.ncbi.nlm.nih.gov/sra/docs/toolkitsoft/" rel="nofollow noreferrer">SRA toolkit</a> available online, to convert your raw reads to fastq format. Follow the steps using command line. It is a very simple command to convert your SRA file to fastq which uses the <em>fastq-dump</em> command.</p>
  
  <p>Further reading: <a href="https://www.ncbi.nlm.nih.gov/books/NBK158900/" rel="nofollow noreferrer">https://www.ncbi.nlm.nih.gov/books/NBK158900/</a></p>
  
  <p>Hope it helps! </p>
   1135: <p>I suggest you to try difference resources, including ArrayExpress. 
  A simple search at <a href="https://www.ebi.ac.uk/arrayexpress/browse.html?keywords=p53&amp;organism=Homo%20sapiens&amp;exptype%5B%5D=&amp;exptype%5B%5D=%22sequencing%20assay%22&amp;array=&amp;page=1&amp;pagesize=500" rel="nofollow noreferrer">ArrayExpress</a> returns multiple experiments which might be of your interest. Just a pointer, ArrayExpress is also synced with GEO.</p>
  