text: user2023: I have the 16S DNA Sequence of a Microorganism. Can I confirm its identity by only running a BLAST or do I have to follow other methods. If so please mention the methods.  
  
  user73: As always with bioinformatics, any reasonable answer will be dependendant on what you want to do with this sequence, and you haven't provided enough information in that regard.
  For most exploratory analysis BLAST is good enough. You don't have to follow any existing methods. The benefit of using NCBI's web BLAST server is that it provides you with a very quick way to find a match from the sequence information in GenBank.
  BLAST does a local alignment. If you have the full 16s sequence, and it's a novel organism, you might get different match results from a program that does global alignment, but then again you might get different results from a different local alignment tool.
  
original_text: 2023: <p>I have the 16S DNA Sequence of a Microorganism. Can I confirm its identity by only running a BLAST or do I have to follow other methods. If so please mention the methods.  </p>
   73: <p>As always with bioinformatics, any reasonable answer will be dependendant on what you want to do with this sequence, and you haven't provided enough information in that regard.</p>
  
  <p>For most exploratory analysis BLAST is good enough. You don't <em>have to</em> follow any existing methods. The benefit of using NCBI's web BLAST server is that it provides you with a very quick way to find a match from the sequence information in GenBank.</p>
  
  <p>BLAST does a local alignment. If you have the full 16s sequence, and it's a novel organism, you <em>might</em> get different match results from a program that does global alignment, but then again you might get different results from a different local alignment tool.</p>
  