text: user1771: By reading Bacher & Kendziorski (2016), I found the following statement:
  
  recent droplet technologies are not yet able to accommodate spike-ins
  
  What's the reason why current droplet technologies cannot use spike-ins? By searching through the available methodologies I found this question on 10X community, but still I cannot understand what is the problem created by using spike-ins with droplet technologies.
  
  Bacher, R. & Kendziorski, C. Design and computational analysis of single-cell RNA-sequencing experiments. Genome Biol. 17, 63 (2016).
  
  
  user3541: I believe it is mostly a cost issue as spike-ins are "expensive" to use given the large number of cells that are/can be sequenced via droplet based methods (as compared to plate-based ones). The 10x link that you provided actually states that this is possible with 10x and Klein et al. used ERCC spike-ins in 953 droplets (Cell. 2015 May 21;161(5):1187-1201. doi: 10.1016/j.cell.2015.04.044.). 
  
original_text: 1771: <p>By reading Bacher &amp; Kendziorski (2016), I found the following statement:</p>
  
  <blockquote>
    <p>recent droplet technologies are not yet able to accommodate spike-ins</p>
  </blockquote>
  
  <p>What's the reason why current droplet technologies cannot use spike-ins? By searching through the available methodologies I found <a href="https://community.10xgenomics.com/t5/Single-Cell-Forum/Thoughts-on-ERCC-Spike-Ins/td-p/207" rel="nofollow noreferrer">this question</a> on 10X community, but still I cannot understand what is the problem created by using spike-ins with droplet technologies.</p>
  
  <ol>
  <li>Bacher, R. &amp; Kendziorski, C. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823857/" rel="nofollow noreferrer">Design and computational analysis of single-cell RNA-sequencing experiments</a>. Genome Biol. 17, 63 (2016).</li>
  </ol>
   3541: <p>I believe it is mostly a cost issue as spike-ins are "expensive" to use given the large number of cells that are/can be sequenced via droplet based methods (as compared to plate-based ones). The 10x link that you provided actually states that this is possible with 10x and Klein et al. used ERCC spike-ins in 953 droplets (Cell. 2015 May 21;161(5):1187-1201. doi: 10.1016/j.cell.2015.04.044.). </p>
  