text: user83: I am experimenting with adding additional reads to the input files I'm giving SOAPdenovo2, and there comes a point where a good contig I've been watching actually stops showing up. Does anyone have a quick answer to why that might happen?
  
  user77: The more reads you add the more errors you add into the assembly. This is because additional duplicate reads don't add nodes/edges to the de Bruijn graph, but those with errors do. By preselecting those reads aligning to your desired contigs, you may be further exacerbating this effect and further hindering SOAPdenovo2's ability to do k-mer correction (after all, the observed k-mer frequency of errors would be higher). This then results in additional incorrect paths through the graph that the assembler has to traverse.
  
original_text: 83: <p>I am experimenting with adding additional reads to the input files I'm giving SOAPdenovo2, and there comes a point where a good contig I've been watching actually stops showing up. Does anyone have a quick answer to why that might happen?</p>
   77: <p>The more reads you add the more errors you add into the assembly. This is because additional duplicate reads don't add nodes/edges to the de Bruijn graph, but those with errors do. By preselecting those reads aligning to your desired contigs, you may be further exacerbating this effect and further hindering SOAPdenovo2's ability to do k-mer correction (after all, the observed k-mer frequency of errors would be higher). This then results in additional incorrect paths through the graph that the assembler has to traverse.</p>
  