text: user8601: I am reading this paper, where the authors mention
  
  We apply SAUCIE to the batch correcting, denoising and clustering of
  an 11-million cell mass cytometry dataset with 180 samples from 40
  subjects in a study of the dengue flavivirus
  
  What exactly is the difference between a “sample” and a “subject” in scRNA-Seq?
  PS: here’s a possibly relevant visual from the paper, but I still couldn’t understand the difference
  
  
  user104: A subject is an individual or donor. You can take multiple samples from the same subject, e.g. from different tissues, different timepoints etc.
  
original_text: 8601: <p>I am reading <a href="https://www.nature.com/articles/s41592-019-0576-7" rel="nofollow noreferrer">this</a> paper, where the authors mention</p>
  <blockquote>
  <p>We apply SAUCIE to the batch correcting, denoising and clustering of
  an 11-million cell mass cytometry dataset with 180 samples from 40
  subjects in a study of the dengue flavivirus</p>
  </blockquote>
  <p>What exactly is the difference between a “sample” and a “subject” in scRNA-Seq?</p>
  <p>PS: here’s a possibly relevant visual from the paper, but I still couldn’t understand the difference
  <a href="https://i.stack.imgur.com/DEUUU.jpg" rel="nofollow noreferrer"><img src="https://i.stack.imgur.com/DEUUU.jpg" alt="enter image description here" /></a></p>
   104: <p>A subject is an individual or donor. You can take multiple samples from the same subject, e.g. from different tissues, different timepoints etc.</p>
  