text: user9449: I have a Seurat object of four cancers and four controls
  > colnames(pbmc@meta.data)
   [1] "orig.ident"       "nCount_RNA"       "nFeature_RNA"     "samples"         
   [5] "Condition"        "Gender"           "percent.mt"       "nCount_SCT"      
   [9] "nFeature_SCT"     "SCT_snn_res.0.75" "seurat_clusters"  "SCT_snn_res.0.5" 
  > unique(pbmc@meta.data$Condition)
  [1] "control" "cancer" 
  > 
  
  
  I want to have a heat map of differentially expressed between cancers and controls like figure 3A in this paper
  https://www.frontiersin.org/articles/10.3389/fgene.2020.610682/full
  
  Do you have any idea please how I can do that? I've looked at the integration tutorial, but can't find the heatmap in it.
  
  user73: See the DoHeatmap function in Seurat, which seems to be what that paper has used:
  https://satijalab.org/seurat/articles/pbmc3k_tutorial.html#cb50
  > DoHeatmap(pbmc, features = myGeneList, group.by="Condition")
  
  
original_text: 9449: <p>I have a Seurat object of four cancers and four controls</p>
  <pre><code>&gt; colnames(pbmc@meta.data)
   [1] &quot;orig.ident&quot;       &quot;nCount_RNA&quot;       &quot;nFeature_RNA&quot;     &quot;samples&quot;         
   [5] &quot;Condition&quot;        &quot;Gender&quot;           &quot;percent.mt&quot;       &quot;nCount_SCT&quot;      
   [9] &quot;nFeature_SCT&quot;     &quot;SCT_snn_res.0.75&quot; &quot;seurat_clusters&quot;  &quot;SCT_snn_res.0.5&quot; 
  &gt; unique(pbmc@meta.data$Condition)
  [1] &quot;control&quot; &quot;cancer&quot; 
  &gt; 
  </code></pre>
  <p><a href="https://i.stack.imgur.com/TbG8d.png" rel="nofollow noreferrer"><img src="https://i.stack.imgur.com/TbG8d.png" alt="enter image description here" /></a></p>
  <p>I want to have a heat map of differentially expressed between cancers and controls like <code>figure 3A</code> in this paper</p>
  <p><a href="https://www.frontiersin.org/articles/10.3389/fgene.2020.610682/full" rel="nofollow noreferrer">https://www.frontiersin.org/articles/10.3389/fgene.2020.610682/full</a></p>
  <p><a href="https://i.stack.imgur.com/ZVaJh.png" rel="nofollow noreferrer"><img src="https://i.stack.imgur.com/ZVaJh.png" alt="enter image description here" /></a></p>
  <p>Do you have any idea please how I can do that? I've looked at the <a href="https://satijalab.org/seurat/articles/integration_introduction.html" rel="nofollow noreferrer">integration tutorial</a>, but can't find the heatmap in it.</p>
   73: <p>See the <code>DoHeatmap</code> function in Seurat, which seems to be what that paper has used:</p>
  <p><a href="https://satijalab.org/seurat/articles/pbmc3k_tutorial.html#cb50" rel="nofollow noreferrer">https://satijalab.org/seurat/articles/pbmc3k_tutorial.html#cb50</a></p>
  <pre><code>&gt; DoHeatmap(pbmc, features = myGeneList, group.by=&quot;Condition&quot;)
  </code></pre>
  