text: user15697: I have about 300 90-bp sequences which I would like to map to a reference genome to make it longer to a 300bp sequence, wherein the 90bp is at the middle.
  Anyone knows what bioinformatics tool I can use for this? Thank you!
  Actual scenario:
  The SNP sequences are from a SNP panel data (90bp sequence, SNP is at the middle). We would like to extend the sequence to 300bp, retaining the SNP at the middle so we can make a sequence capture panel of the SNP.
  
  user1540: If you have a SNP panel then you have the coordinates on the genome. You can use tools such as bedtools slop to expand the coordinates to the desired length and then extract those regions from the target genome using bedtools getfasta.
  
original_text: 15697: <p>I have about 300 90-bp sequences which I would like to map to a reference genome to make it longer to a 300bp sequence, wherein the 90bp is at the middle.</p>
  <p>Anyone knows what bioinformatics tool I can use for this? Thank you!</p>
  <p><em>Actual scenario:</em>
  The SNP sequences are from a SNP panel data (90bp sequence, SNP is at the middle). We would like to extend the sequence to 300bp, retaining the SNP at the middle so we can make a sequence capture panel of the SNP.</p>
   1540: <p>If you have a SNP panel then you have the coordinates on the genome. You can use tools such as <a href="https://bedtools.readthedocs.io/en/latest/content/tools/slop.html" rel="nofollow noreferrer">bedtools slop</a> to expand the coordinates to the desired length and then extract those regions from the target genome using <a href="https://bedtools.readthedocs.io/en/latest/content/tools/getfasta.html" rel="nofollow noreferrer">bedtools getfasta</a>.</p>
  