text: user14551: I got this error during execution, pls guide me.
  vep --cache -i xxxx.vcf -o xxxxx_new.vcf
  
  Possible precedence issue with control flow operator at /software/xxxx_pkgs/envs/ensemble-vep/lib/site_perl/5.26.2/Bio/DB/IndexedBase.pm line 805.
  DBI connect('host=ensembldb.ensembl.org;port=3306','anonymous',...) failed: Can't connect to MySQL server on 'ensembldb.ensembl.org' (110) at /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/Bio/EnsEMBL/Registry.pm line 1771.
  
  -------------------- EXCEPTION --------------------
  MSG: Cannot connect to the Ensembl MySQL server at ensembldb.ensembl.org:3306; check your settings & DBI error message: Can't connect to MySQL server on 'ensembldb.ensembl.org' (110)
  STACK Bio::EnsEMBL::Registry::load_registry_from_db /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/Bio/EnsEMBL/Registry.pm:1771
  STACK Bio::EnsEMBL::VEP::BaseVEP::registry /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/modules/Bio/EnsEMBL/VEP/BaseVEP.pm:163
  STACK Bio::EnsEMBL::VEP::BaseRunner::setup_db_connection /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/modules/Bio/EnsEMBL/VEP/BaseRunner.pm:80
  STACK Bio::EnsEMBL::VEP::Runner::init /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/modules/Bio/EnsEMBL/VEP/Runner.pm:72
  STACK Bio::EnsEMBL::VEP::Runner::run /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/modules/Bio/EnsEMBL/VEP/Runner.pm:111
  STACK toplevel /software//envs/ensemble-vep/bin/vep:209
  Ensembl API version = 88
  ```
  
  
  user10775: You might prefer to run VEP in offline mode:
  
  Using --cache (without --offline) uses the local cache on disk to
  fetch most annotations, but allows database connections for some
  features (see cache limitations)
  
  If you need HGVS names in your output, you'll also need to supply a FASTA file using --fasta:
  
  --offline
  Enable offline mode. No database connections will be made, and a cache file or GFF/GTF file is required for annotation. Add
  --refseq to use the refseq cache (if installed). Not used by default.
  
  
  --fasta [file|dir]
  Specify a FASTA file or a directory containing FASTA files to use to look up reference sequence. The first time you run VEP with this parameter an index will be built which can take a few minutes. This is required if fetching HGVS annotations (--hgvs) or checking reference sequences (--check_ref) in offline mode (--offline), and optional with some performance increase in cache mode (--cache). See documentation for more details. Not used by default.
  
  
original_text: 14551: <p>I got this error during execution, pls guide me.</p>
  <pre><code>vep --cache -i xxxx.vcf -o xxxxx_new.vcf
  
  Possible precedence issue with control flow operator at /software/xxxx_pkgs/envs/ensemble-vep/lib/site_perl/5.26.2/Bio/DB/IndexedBase.pm line 805.
  DBI connect('host=ensembldb.ensembl.org;port=3306','anonymous',...) failed: Can't connect to MySQL server on 'ensembldb.ensembl.org' (110) at /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/Bio/EnsEMBL/Registry.pm line 1771.
  
  -------------------- EXCEPTION --------------------
  MSG: Cannot connect to the Ensembl MySQL server at ensembldb.ensembl.org:3306; check your settings &amp; DBI error message: Can't connect to MySQL server on 'ensembldb.ensembl.org' (110)
  STACK Bio::EnsEMBL::Registry::load_registry_from_db /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/Bio/EnsEMBL/Registry.pm:1771
  STACK Bio::EnsEMBL::VEP::BaseVEP::registry /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/modules/Bio/EnsEMBL/VEP/BaseVEP.pm:163
  STACK Bio::EnsEMBL::VEP::BaseRunner::setup_db_connection /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/modules/Bio/EnsEMBL/VEP/BaseRunner.pm:80
  STACK Bio::EnsEMBL::VEP::Runner::init /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/modules/Bio/EnsEMBL/VEP/Runner.pm:72
  STACK Bio::EnsEMBL::VEP::Runner::run /software/xxxx_pkgs/envs/ensemble-vep/share/ensembl-vep-88.9-1/modules/Bio/EnsEMBL/VEP/Runner.pm:111
  STACK toplevel /software//envs/ensemble-vep/bin/vep:209
  Ensembl API version = 88
  <span class="math-container">```</span>
  </code></pre>
   10775: <p>You might prefer to run <a href="https://m.ensembl.org/info/docs/tools/vep/script/vep_options.html" rel="nofollow noreferrer">VEP</a> in offline mode:</p>
  <blockquote>
  <p>Using <code>--cache</code> (without <code>--offline</code>) uses the local cache on disk to
  fetch most annotations, but allows database connections for some
  features (see <a href="https://m.ensembl.org/info/docs/tools/vep/script/vep_cache.html#limitations" rel="nofollow noreferrer">cache limitations</a>)</p>
  </blockquote>
  <p>If you need HGVS names in your output, you'll also need to supply a FASTA file using <code>--fasta</code>:</p>
  <blockquote>
  <p><a href="https://m.ensembl.org/info/docs/tools/vep/script/vep_options.html#opt_offline" rel="nofollow noreferrer">--offline</a></p>
  <p>Enable offline mode. No database connections will be made, and a cache file or GFF/GTF file is required for annotation. Add
  --refseq to use the refseq cache (if installed). Not used by default.</p>
  </blockquote>
  <blockquote>
  <p><a href="https://m.ensembl.org/info/docs/tools/vep/script/vep_options.html#opt_fasta" rel="nofollow noreferrer">--fasta [file|dir]</a></p>
  <p>Specify a FASTA file or a directory containing FASTA files to use to look up reference sequence. The first time you run VEP with this parameter an index will be built which can take a few minutes. This is required if fetching HGVS annotations (--hgvs) or checking reference sequences (--check_ref) in offline mode (--offline), and optional with some performance increase in cache mode (--cache). See documentation for more details. Not used by default.</p>
  </blockquote>
  