text: user285: Im quoting the methods which was used 
  
  We performed a bioinformatics analysis to identify potential CpG
    islands in the genomic sequences of RANKL and OPG genes. The USCS
    Human Genome Browser public database (genome.ucsc.edu), the Primer
    Express software (available from Applied Biosystems), and CpG Island
    Explorer software (cpgie.sourceforge.net) were used to explore the
    nucleo- tide sequences surrounding de transcription start site (TSS)
    of RANKL isoforms I and II (NG_008990.1 Ref Seq Gene), and OPG
    (NG_012202.1 Ref Seq Gene). A (C + G)/total bases ratio
  
  0.5 and CpG observed/CpG expected ratio >0.6 were used as criteria.
  
  
  Im looking for the region which they have used 
  This is the paper 
  "Role of DNA methylation in the regulation of the
  RANKL-OPG system in human bone"
  
  I tried using ucsc to check the region but didn't get much. The idea is to find the region to design the primer for the same
  How can i get the region from ucsc ,the CpG island around the TSS of the above mentioned genes which are RANKL and OPG
  Any suggestion or help would be highly appreciated 
  
  user240: It's likely that they accomplished this by using the UCSC Table Browser to:
  
  Find the loci of the two isoforms you mention
  Finding CpG islands (Group = Regulation, Track = CpG Islands) near those transcription start sites
  Design primers using Applied Biosystem's software
  
  Alternatively, they list the exact primers used in Table 1.
  They don't detail exactly how they did it, so if you have specific questions it might be worth it to email the corresponding author
  
original_text: 285: <p>Im quoting the methods which was used </p>
  
  <blockquote>
    <p>We performed a bioinformatics analysis to identify potential CpG
    islands in the genomic sequences of RANKL and OPG genes. The USCS
    Human Genome Browser public database (genome.ucsc.edu), the Primer
    Express software (available from Applied Biosystems), and CpG Island
    Explorer software (cpgie.sourceforge.net) were used to explore the
    nucleo- tide sequences surrounding de transcription start site (TSS)
    of RANKL isoforms I and II (NG_008990.1 Ref Seq Gene), and OPG
    (NG_012202.1 Ref Seq Gene). A (C + G)/total bases ratio</p>
    
    <blockquote>
      <p>0.5 and CpG observed/CpG expected ratio >0.6 were used as criteria.</p>
    </blockquote>
  </blockquote>
  
  <p>Im looking for the region which they have used </p>
  
  <p>This is the paper </p>
  
  <pre><code>"Role of DNA methylation in the regulation of the
  RANKL-OPG system in human bone"
  </code></pre>
  
  <p>I tried using ucsc to check the region but didn't get much. The idea is to find the region to design the primer for the same</p>
  
  <p>How can i get the region from ucsc ,the CpG island around the TSS of the above mentioned genes which are RANKL and OPG</p>
  
  <p>Any suggestion or help would be highly appreciated </p>
   240: <p>It's likely that they accomplished this by using the <a href="http://genome.ucsc.edu/cgi-bin/hgTables" rel="nofollow noreferrer">UCSC Table Browser</a> to:</p>
  
  <ol>
  <li>Find the loci of the two isoforms you mention</li>
  <li>Finding CpG islands (Group = Regulation, Track = CpG Islands) near those transcription start sites</li>
  <li>Design primers using Applied Biosystem's software</li>
  </ol>
  
  <p>Alternatively, they list the exact primers used in <a href="https://www.tandfonline.com/action/downloadTable?id=T1&amp;doi=10.4161%2Fepi.7.1.18753&amp;downloadType=CSV" rel="nofollow noreferrer">Table 1</a>.</p>
  
  <p>They don't detail exactly how they did it, so if you have specific questions it might be worth it to email the corresponding author</p>
  