text: user-1: working on lung cancer project and need to find out which genes are deferentially expressed and which are not. for that i a have downloaded miRNA data set from GEO id is GSE110907. And the file i got has to variable gene_id and read counts. from that i have to derive p value from that data/file.
  
  user3541: I would start by re-formulating the question in this post: Your end-goal seems to the differential expression analysis (DEA) of your samples (and you have to have to levels for this like treatment-control, mutant-WT, etc ...). P-values are context-dependent and should be calculated based on your research question/hypothesis.
  DEA has been addressed by many researchers and R has a lot of packages to help you with this; DESeq2, edgeR, limma, etc ...
  I would go to the Biconductor website, search for one of the three above, and go through their rich vignettes and would come back here for specific questions.
  
original_text: -1: <p>working on lung cancer project and need to find out which genes are deferentially expressed and which are not. for that i a have downloaded miRNA data set from GEO id is GSE110907. And the file i got has to variable gene_id and read counts. from that i have to derive p value from that data/file.<a href="https://i.stack.imgur.com/osaNe.jpg" rel="nofollow noreferrer"><img src="https://i.stack.imgur.com/osaNe.jpg" alt="enter image description here"></a></p>
   3541: <p>I would start by re-formulating the question in this post: Your end-goal seems to the differential expression analysis (DEA) of your samples (and you have to have to levels for this like treatment-control, mutant-WT, etc ...). P-values are context-dependent and should be calculated based on your research question/hypothesis.</p>
  
  <p>DEA has been addressed by many researchers and <code>R</code> has a lot of packages to help you with this; <code>DESeq2</code>, <code>edgeR</code>, <code>limma</code>, etc ...</p>
  
  <p>I would go to the <code>Biconductor</code> website, search for one of the three above, and go through their rich <strong>vignettes</strong> and would come back here for <strong>specific</strong> questions.</p>
  