text: user3061: Does Prokka do six-frame genome annotations? The corresponding publication does not mention it.
  
  Seemann, Torsten. 2014. “Prokka: Rapid Prokaryotic Genome Annotation.” Bioinformatics  30 (14): 2068–69.
  
  
  user104: Yes, Prokka will consider all six-frames, the gene prediction is actually done with Prodigal, paper:
  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848648/
  github:
  https://github.com/hyattpd/Prodigal
  
original_text: 3061: <p>Does Prokka do six-frame genome annotations? The corresponding publication does not mention it.</p>
  
  <blockquote>
    <p>Seemann, Torsten. 2014. “Prokka: Rapid Prokaryotic Genome Annotation.” Bioinformatics  30 (14): 2068–69.</p>
  </blockquote>
   104: <p>Yes, Prokka will consider all six-frames, the gene prediction is actually done with Prodigal, paper:
  <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848648/" rel="noreferrer">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848648/</a></p>
  
  <p>github:
  <a href="https://github.com/hyattpd/Prodigal" rel="noreferrer">https://github.com/hyattpd/Prodigal</a></p>
  