text: user16335: This question has also been asked on Biostars
  I am trying to create a Seurat object using the package "Seurat". When I am reading my raw files using the function Read10X, I am getting this error
  Error in dimnamesGets(x, value) : length of Dimnames[[1]] (45948) is not equal to Dim[1] (45947).
  
  I am unsure how to resolve this issue.
  R sessionInfo:
  R version 4.1.2 (2021-11-01)
  Platform: x86_64-w64-mingw32/x64 (64-bit)
  Running under: Windows 10 x64 (build 19044)
  
  Matrix products: default
  
  locale:
  [1] LC_COLLATE=English_United States.1252  LC_CTYPE=English_United States.1252
  [3] LC_MONETARY=English_United States.1252 LC_NUMERIC=C
  [5] LC_TIME=English_United States.1252
  
  attached base packages:
  [1] stats     graphics  grDevices utils     datasets  methods   base
  
  other attached packages:
  [1] sp_1.5-0           SeuratObject_4.1.1 Seurat_4.1.1       Matrix_1.5-1
  
  loaded via a namespace (and not attached):
    [1] nlme_3.1-153          spatstat.sparse_2.1-1 matrixStats_0.62.0    RcppAnnoy_0.0.19
    [5] RColorBrewer_1.1-3    httr_1.4.4            sctransform_0.3.4     tools_4.1.2
    [9] utf8_1.2.2            R6_2.5.1              irlba_2.3.5           rpart_4.1-15
   [13] KernSmooth_2.23-20    uwot_0.1.14           mgcv_1.8-38           rgeos_0.5-9
   [17] DBI_1.1.3             lazyeval_0.2.2        colorspace_2.0-3      tidyselect_1.1.2
   [21] gridExtra_2.3         compiler_4.1.2        progressr_0.11.0      cli_3.4.0
   [25] plotly_4.10.0         scales_1.2.1          lmtest_0.9-40         spatstat.data_2.2-0
   [29] ggridges_0.5.3        pbapply_1.5-0         goftest_1.2-3         stringr_1.4.1
   [33] digest_0.6.29         spatstat.utils_2.3-1  pkgconfig_2.0.3       htmltools_0.5.3
   [37] parallelly_1.32.1     fastmap_1.1.0         htmlwidgets_1.5.4     rlang_1.0.5
   [41] rstudioapi_0.14       shiny_1.7.2           generics_0.1.3        zoo_1.8-10
   [45] jsonlite_1.8.0        spatstat.random_2.2-0 ica_1.0-3             dplyr_1.0.10
   [49] magrittr_2.0.3        patchwork_1.1.2       Rcpp_1.0.9            munsell_0.5.0
   [53] fansi_1.0.3           abind_1.4-5           reticulate_1.26       lifecycle_1.0.2
   [57] stringi_1.7.6         MASS_7.3-54           Rtsne_0.16            plyr_1.8.7
   [61] grid_4.1.2            parallel_4.1.2        listenv_0.8.0         promises_1.2.0.1
   [65] ggrepel_0.9.1         deldir_1.0-6          miniUI_0.1.1.1        lattice_0.20-45
   [69] cowplot_1.1.1         splines_4.1.2         tensor_1.5            pillar_1.8.1
   [73] igraph_1.3.4          spatstat.geom_2.4-0   future.apply_1.9.1    reshape2_1.4.4
   [77] codetools_0.2-18      leiden_0.4.3          glue_1.6.2            data.table_1.14.2
   [81] png_0.1-7             vctrs_0.4.1           httpuv_1.6.6          polyclip_1.10-0
   [85] gtable_0.3.1          RANN_2.6.1            purrr_0.3.4           spatstat.core_2.4-4
   [89] tidyr_1.2.1           scattermore_0.8       future_1.28.0         assertthat_0.2.1
   [93] ggplot2_3.3.6         mime_0.12             xtable_1.8-4          later_1.3.0
   [97] survival_3.2-13       viridisLite_0.4.1     tibble_3.1.8          cluster_2.1.2
  [101] globals_0.16.1        fitdistrplus_1.1-8    ellipsis_0.3.2        ROCR_1.0-11
  ```
  
  
  user15931: Check the length of your files. The error clearly says that there is a difference in the length of your files. Your file length should be equal.
  I think the problem may be the presence of a header in one of your files. Removing the header may solve the problem.
  
  user16342: There is a mismatch of 1 line between the several files, suggesting that probably either the barcodes of features file has a header line which is not standard. Remove any header lines from these files.
  
original_text: 16335: <p><em>This question has also been asked on <a href="https://www.biostars.org/p/9542032/" rel="nofollow noreferrer">Biostars</a></em></p>
  <p>I am trying to create a Seurat object using the package &quot;Seurat&quot;. When I am reading my raw files using the function <code>Read10X</code>, I am getting this error</p>
  <pre class="lang-r prettyprint-override"><code>Error in dimnamesGets(x, value) : length of Dimnames[[1]] (45948) is not equal to Dim[1] (45947).
  </code></pre>
  <p>I am unsure how to resolve this issue.</p>
  <p>R sessionInfo:</p>
  <pre class="lang-r prettyprint-override"><code>R version 4.1.2 (2021-11-01)
  Platform: x86_64-w64-mingw32/x64 (64-bit)
  Running under: Windows 10 x64 (build 19044)
  
  Matrix products: default
  
  locale:
  [1] LC_COLLATE=English_United States.1252  LC_CTYPE=English_United States.1252
  [3] LC_MONETARY=English_United States.1252 LC_NUMERIC=C
  [5] LC_TIME=English_United States.1252
  
  attached base packages:
  [1] stats     graphics  grDevices utils     datasets  methods   base
  
  other attached packages:
  [1] sp_1.5-0           SeuratObject_4.1.1 Seurat_4.1.1       Matrix_1.5-1
  
  loaded via a namespace (and not attached):
    [1] nlme_3.1-153          spatstat.sparse_2.1-1 matrixStats_0.62.0    RcppAnnoy_0.0.19
    [5] RColorBrewer_1.1-3    httr_1.4.4            sctransform_0.3.4     tools_4.1.2
    [9] utf8_1.2.2            R6_2.5.1              irlba_2.3.5           rpart_4.1-15
   [13] KernSmooth_2.23-20    uwot_0.1.14           mgcv_1.8-38           rgeos_0.5-9
   [17] DBI_1.1.3             lazyeval_0.2.2        colorspace_2.0-3      tidyselect_1.1.2
   [21] gridExtra_2.3         compiler_4.1.2        progressr_0.11.0      cli_3.4.0
   [25] plotly_4.10.0         scales_1.2.1          lmtest_0.9-40         spatstat.data_2.2-0
   [29] ggridges_0.5.3        pbapply_1.5-0         goftest_1.2-3         stringr_1.4.1
   [33] digest_0.6.29         spatstat.utils_2.3-1  pkgconfig_2.0.3       htmltools_0.5.3
   [37] parallelly_1.32.1     fastmap_1.1.0         htmlwidgets_1.5.4     rlang_1.0.5
   [41] rstudioapi_0.14       shiny_1.7.2           generics_0.1.3        zoo_1.8-10
   [45] jsonlite_1.8.0        spatstat.random_2.2-0 ica_1.0-3             dplyr_1.0.10
   [49] magrittr_2.0.3        patchwork_1.1.2       Rcpp_1.0.9            munsell_0.5.0
   [53] fansi_1.0.3           abind_1.4-5           reticulate_1.26       lifecycle_1.0.2
   [57] stringi_1.7.6         MASS_7.3-54           Rtsne_0.16            plyr_1.8.7
   [61] grid_4.1.2            parallel_4.1.2        listenv_0.8.0         promises_1.2.0.1
   [65] ggrepel_0.9.1         deldir_1.0-6          miniUI_0.1.1.1        lattice_0.20-45
   [69] cowplot_1.1.1         splines_4.1.2         tensor_1.5            pillar_1.8.1
   [73] igraph_1.3.4          spatstat.geom_2.4-0   future.apply_1.9.1    reshape2_1.4.4
   [77] codetools_0.2-18      leiden_0.4.3          glue_1.6.2            data.table_1.14.2
   [81] png_0.1-7             vctrs_0.4.1           httpuv_1.6.6          polyclip_1.10-0
   [85] gtable_0.3.1          RANN_2.6.1            purrr_0.3.4           spatstat.core_2.4-4
   [89] tidyr_1.2.1           scattermore_0.8       future_1.28.0         assertthat_0.2.1
   [93] ggplot2_3.3.6         mime_0.12             xtable_1.8-4          later_1.3.0
   [97] survival_3.2-13       viridisLite_0.4.1     tibble_3.1.8          cluster_2.1.2
  [101] globals_0.16.1        fitdistrplus_1.1-8    ellipsis_0.3.2        ROCR_1.0-11
  <span class="math-container">```</span>
  </code></pre>
   15931: <p>Check the length of your files. The error clearly says that there is a difference in the length of your files. Your file length should be equal.</p>
  <p>I think the problem may be the presence of a header in one of your files. Removing the header may solve the problem.</p>
   16342: <p>There is a mismatch of 1 line between the several files, suggesting that probably either the barcodes of features file has a header line which is not standard. Remove any header lines from these files.</p>
  