text: user1075: I am sequencing a whole genome on MinION. I have used this flow cell for 6 hours for the phage lambda control. That gave me around 300,000 reads. I started a 48h sequencing run on the same flow cell after washing it. The sequencing has been going on for 1 day and 21 hours. It is not producing any reads at the moment (Strand = 0). The number of single pores is also 0. Can I "Stop acquisition" without negatively affecting the results?
  The image below is a screenshot taken from MinKNOW, the software used to control the MinION.
  
  
  user3576: Yes, you can stop the run and it should not affect the base calling. We normally do the runs and then do the base calling after the run has completed.
  
  user2085: Yes, you can click "stop acquisition" and your run won't be negatively affected. All of the reads are saved as they are generated. I am not sure how this will impact live basecalling though if that is something that you do.
  
original_text: 1075: <p>I am sequencing a whole genome on MinION. I have used this flow cell for 6 hours for the phage lambda control. That gave me around 300,000 reads. I started a 48h sequencing run on the same flow cell after washing it. The sequencing has been going on for 1 day and 21 hours. It is not producing any reads at the moment (Strand = 0). The number of single pores is also 0. Can I "Stop acquisition" without negatively affecting the results?</p>
  
  <p>The image below is a screenshot taken from MinKNOW, the software used to control the MinION.</p>
  
  <p><a href="https://i.stack.imgur.com/D5FuH.png" rel="nofollow noreferrer"><img src="https://i.stack.imgur.com/D5FuH.png" alt="Pore description and read summary"></a></p>
   3576: <p>Yes, you can stop the run and it should not affect the base calling. We normally do the runs and then do the base calling after the run has completed.</p>
   2085: <p>Yes, you can click "stop acquisition" and your run won't be negatively affected. All of the reads are saved as they are generated. I am not sure how this will impact live basecalling though if that is something that you do.</p>
  