text: user6471: I am trying to find sequence homology between viral sequences and my protein of interest. I have the sequences of their epitopes which varies from 5 to 500 amino acids long. For shorter sequences, it is recommended to use blastp-short but I am confused until which length it is okay to run blastp?
  For shorter sequences, what are the parameters needs to be added for effective results?
  blastp -task blastp-short -query POI.fasta -db virus.fasta -out output.txt -outfmt 6 -evalue 20000
  
  user4282: You should increase the -evalue 10 default option to a higher value because this value it's adjusted to the sequence database size. Check this table, maybe this can help you to decide which parameters are the best option for you.
      OPTION      TASK          DEFAUL    DESCRIPTION
      word_size   blastp        3         Word size of initial match.
      word_size   blastp-short  2         Word size of initial match.
      gapopen     blastp        1         Cost to open a gap.
      gapopen     blastp-short  9         Cost to open a gap.
      gapextend   blastp        1         Cost to extend a gap.
      gapextend   blastp-short  1         Cost to extend a gap.
      matrix      blastp        BLOSUM62  Scoring matrix name.
      matrix      blastp-short  PAM30     Scoring matrix name.
      threshold   blastp        11        Minimum score to add a word to the BLAST lookup table.
      threshold   blastp-short  16        Minimum score to add a word to the BLAST lookup table.
      window_size blastp        40        Multiple hits window size, use 0 to specify 1-hit algorithm.
      window_size blastp-short  15        Multiple hits window size, use 0 to specify 1-hit algorithm.
  
  
original_text: 6471: <p>I am trying to find sequence homology between viral sequences and my protein of interest. I have the sequences of their epitopes which varies from 5 to 500 amino acids long. For shorter sequences, it is recommended to use <code>blastp-short</code> but I am confused until which length it is okay to run <code>blastp</code>?</p>
  
  <p>For shorter sequences, what are the parameters needs to be added for effective results?</p>
  
  <p><code>blastp -task blastp-short -query POI.fasta -db virus.fasta -out output.txt -outfmt 6 -evalue 20000</code></p>
   4282: <p>You should increase the <code>-evalue 10</code> default option to a higher value because this value it's adjusted to the sequence database size. Check this table, maybe this can help you to decide which parameters are the best option for you.</p>
  
  <pre><code>    OPTION      TASK          DEFAUL    DESCRIPTION
      word_size   blastp        3         Word size of initial match.
      word_size   blastp-short  2         Word size of initial match.
      gapopen     blastp        1         Cost to open a gap.
      gapopen     blastp-short  9         Cost to open a gap.
      gapextend   blastp        1         Cost to extend a gap.
      gapextend   blastp-short  1         Cost to extend a gap.
      matrix      blastp        BLOSUM62  Scoring matrix name.
      matrix      blastp-short  PAM30     Scoring matrix name.
      threshold   blastp        11        Minimum score to add a word to the BLAST lookup table.
      threshold   blastp-short  16        Minimum score to add a word to the BLAST lookup table.
      window_size blastp        40        Multiple hits window size, use 0 to specify 1-hit algorithm.
      window_size blastp-short  15        Multiple hits window size, use 0 to specify 1-hit algorithm.
  </code></pre>
  