text: user4595: Sorry, 
  I have analysed my time series single cell data with Scuba algorithm but that says that there is no bifurcations in my data and outputted a single chain of 4 states with no branching like below figure
  
  Sorry, I googled but I am not quite sure what this means
  Any suggestion please?
  Thanks a lot
  
  user235: The most obvious interpretation is that the four cell lines are related in a linear pathway rather than a branching one. 
  
original_text: 4595: <p>Sorry, </p>
  
  <p>I have analysed my time series single cell data with Scuba algorithm but that says that there is no bifurcations in my data and outputted a single chain of 4 states with no branching like below figure</p>
  
  <p><a href="https://i.stack.imgur.com/RZKSo.jpg" rel="nofollow noreferrer"><img src="https://i.stack.imgur.com/RZKSo.jpg" alt="enter image description here"></a></p>
  
  <p>Sorry, I googled but I am not quite sure what this means</p>
  
  <p>Any suggestion please?</p>
  
  <p>Thanks a lot</p>
   235: <p>The most obvious interpretation is that the four cell lines are related in a linear pathway rather than a branching one. </p>
  