text: user1684: Single stranded cDNA is used by race pcr and rt-pcr while next-gen sequencing (Illumina) requires double stranded cDNA. What is the reason behind this ? 
  
  user77: Sequencing also requires single stranded DNA, otherwise the sequencing primers can’t bind. Sure there are steps in the protocol where double stranded material exists, but that’s transient. 
  
original_text: 1684: <p>Single stranded cDNA is used by race pcr and rt-pcr while next-gen sequencing (Illumina) requires double stranded cDNA. What is the reason behind this ? </p>
   77: <p>Sequencing also requires single stranded DNA, otherwise the sequencing primers can’t bind. Sure there are steps in the protocol where double stranded material exists, but that’s transient. </p>
  