text: user4595: Supposing upcoming RNA-seq for 3 conditions and 3 replications for each, how much is data storage requirements?
  
  user77: The breakdown would be:
  
  1.5-2 GB per sample for fastq files.
  ~3GB per BAM file, depending on the aligner
  
  So ~5GB per BAM file, which is about 50GB total. That can vary by maybe 20%, depending on how compressible the results are (I rearrange our fastq files to make them more compressible, so I'm probably underestimating what others would see).
  Obviously using Salmon/Kallisto would halve the storage requirements. Using CRAM would also decrease things, though obviously fewer tools support it.
  
original_text: 4595: <p>Supposing upcoming RNA-seq for 3 conditions and 3 replications for each, how much is data storage requirements?</p>
   77: <p>The breakdown would be:</p>
  
  <ul>
  <li>1.5-2 GB per sample for fastq files.</li>
  <li>~3GB per BAM file, depending on the aligner</li>
  </ul>
  
  <p>So ~5GB per BAM file, which is about 50GB total. That can vary by maybe 20%, depending on how compressible the results are (I rearrange our fastq files to make them more compressible, so I'm probably underestimating what others would see).</p>
  
  <p>Obviously using Salmon/Kallisto would halve the storage requirements. Using CRAM would also decrease things, though obviously fewer tools support it.</p>
  