text: user4595: I have downloaded log2(TPM/10+1) values of 11,548 genes and 9609 cells from GSE146026 (10x) in tsv format as the raw data is not available
  I see patient IDs, cell barcodes, genes, even assigned clusters are here
  > dput(head(a[1:5,1:5]))
  structure(list(Cell_ID = c("10x_barcode", "patient", "time", 
  "sample_ID", "clst"), X10x_1 = c("10x_3288_t1_AAACATACCTTCCG-1", 
  "5", "1", "3288.1", "1"), X10x_2 = c("10x_3288_t1_AAACATACTCCTAT-1", 
  "5", "1", "3288.1", "1"), X10x_3 = c("10x_3288_t1_AAACATTGAACTGC-1", 
  "5", "1", "3288.1", "1"), X10x_4 = c("10x_3288_t1_AAACATTGCTGACA-1", 
  "5", "1", "3288.1", "2")), row.names = c(NA, 5L), class = "data.frame")
  >
  
  I want to make a Seurat object of that but really I don't know how to do that
  I have tried this
      CreateSeuratObject(getwd(),gene.column = 2,cell.column = 1,unique.features = TRUE,strip.suffix = FALSE)
  
  But get the following error:
  Error in CreateAssayObject(counts = counts, min.cells = min.cells, min.features = min.features) : 
    No cell names (colnames) names present in the input matrix
  
  Anybody have ever dealt with such a case to help me please?
  
  user3541: The function you need is CreateSeuratObject() and not Read10X() as you start from TPMs.
  
original_text: 4595: <p>I have downloaded <code>log2(TPM/10+1)</code> values of <code>11,548 genes</code> and <code>9609 cells</code> from <code>GSE146026</code> (10x) in <code>tsv format</code> as the raw data is not available</p>
  <p>I see patient IDs, cell barcodes, genes, even assigned clusters are here</p>
  <pre><code>&gt; dput(head(a[1:5,1:5]))
  structure(list(Cell_ID = c(&quot;10x_barcode&quot;, &quot;patient&quot;, &quot;time&quot;, 
  &quot;sample_ID&quot;, &quot;clst&quot;), X10x_1 = c(&quot;10x_3288_t1_AAACATACCTTCCG-1&quot;, 
  &quot;5&quot;, &quot;1&quot;, &quot;3288.1&quot;, &quot;1&quot;), X10x_2 = c(&quot;10x_3288_t1_AAACATACTCCTAT-1&quot;, 
  &quot;5&quot;, &quot;1&quot;, &quot;3288.1&quot;, &quot;1&quot;), X10x_3 = c(&quot;10x_3288_t1_AAACATTGAACTGC-1&quot;, 
  &quot;5&quot;, &quot;1&quot;, &quot;3288.1&quot;, &quot;1&quot;), X10x_4 = c(&quot;10x_3288_t1_AAACATTGCTGACA-1&quot;, 
  &quot;5&quot;, &quot;1&quot;, &quot;3288.1&quot;, &quot;2&quot;)), row.names = c(NA, 5L), class = &quot;data.frame&quot;)
  &gt;
  </code></pre>
  <p>I want to make a Seurat object of that but really I don't know how to do that</p>
  <p>I have tried this</p>
  <pre><code>    CreateSeuratObject(getwd(),gene.column = 2,cell.column = 1,unique.features = TRUE,strip.suffix = FALSE)
  </code></pre>
  <p>But get the following error:</p>
  <pre><code>Error in CreateAssayObject(counts = counts, min.cells = min.cells, min.features = min.features) : 
    No cell names (colnames) names present in the input matrix
  </code></pre>
  <p>Anybody have ever dealt with such a case to help me please?</p>
   3541: <p>The function you need is <code>CreateSeuratObject()</code> and not <code>Read10X()</code> as you start from TPMs.</p>
  