text: user9234: Im trying to change the active.ident to another column in metadata but this error keeps popping up! I recently upgraded to R version 4.0.2 from 3.6.1 The older version was working but the new one isn't. I don't understand how to fix it?
  M@active.ident <- M@meta.data$status
  Error in (function (cl, name, valueClass)  : 
  assignment of an object of class “character” is not valid for @‘active.ident’ in an object of class 
  “Seurat”; is(value, "factor") is not TRUE
  
  head(M@meta.data) 
          orig.ident nCount_RNA nFeature_RNA percent.mito   status  RNA_snn_res.0.6 seurat_clusters 
  BC03_03       BC03   998414.7         3356   0.07869098 nonTumor                0               0
  BC03_09       BC03   999853.6         7616   0.12964336  primary                0               0
  BC03_13       BC03   998206.5         8457   0.17672205  primary                0               0
  BC03_17       BC03   999535.1         7494   0.11323008  primary                0               0
  
  I want to run the UMAP with the clusters named according to the status and not seurat clusters.However, the code isnt working..
  
  user3541: M <- SetIdent(M, value = "status")
  or more explicitly
  M <- SetIdent(M, value = M@meta.data$status)
  You can also use the group.by argument of UMAPPlot() or other plotting functions from Seurat for that matter.
  
original_text: 9234: <p>Im trying to change the active.ident to another column in metadata but this error keeps popping up! I recently upgraded to R version 4.0.2 from 3.6.1 The older version was working but the new one isn't. I don't understand how to fix it?</p>
  <pre><code>M@active.ident &lt;- M@meta.data$status
  Error in (function (cl, name, valueClass)  : 
  assignment of an object of class “character” is not valid for @‘active.ident’ in an object of class 
  “Seurat”; is(value, &quot;factor&quot;) is not TRUE
  
  head(M@meta.data) 
          orig.ident nCount_RNA nFeature_RNA percent.mito   status  RNA_snn_res.0.6 seurat_clusters 
  BC03_03       BC03   998414.7         3356   0.07869098 nonTumor                0               0
  BC03_09       BC03   999853.6         7616   0.12964336  primary                0               0
  BC03_13       BC03   998206.5         8457   0.17672205  primary                0               0
  BC03_17       BC03   999535.1         7494   0.11323008  primary                0               0
  </code></pre>
  <p>I want to run the UMAP with the clusters named according to the status and not seurat clusters.However, the code isnt working..</p>
   3541: <p><code>M &lt;- SetIdent(M, value = &quot;status&quot;)</code></p>
  <p>or more explicitly</p>
  <p><code>M &lt;- SetIdent(M, value = M@meta.data$status)</code></p>
  <p>You can also use the <code>group.by</code> argument of <code>UMAPPlot()</code> or other plotting functions from <code>Seurat</code> for that matter.</p>
  