original_docstring: 
          Get the Tm using simple calculation.
          
docstring: Get the Tm using simple calculation.
docstring_tokens: Get the Tm using simple calculation .
code: def Tm_simple(self):
          """
          Get the Tm using simple calculation.
          """
          
          Tm_simple_params_short = {
              'A': 2,
              'T': 2,
              'C': 4,
              'G': 4
              }
  
          Tm_simple_params_long = {
              'A': 64.9,
              'T': 64.9,
              'G': 64.9 + 41,
              'C': 64.9 + 41
              }
          
          if len(self.seq) < 14:
              return sum([Tm_simple_params_short[nucleotide] * self.nucleotide_count()[nucleotide] for nucleotide in ['A', 'T', 'C', 'G']])
          
          else:
              return (sum([Tm_simple_params_long[nucleotide] * self.nucleotide_count()[nucleotide] for nucleotide in ['A', 'T', 'C', 'G']])- 41 *16.4)/len(self.seq)
code_tokens: def Tm_simple ( self ) : Tm_simple_params_short = { 'A' : 2 , 'T' : 2 , 'C' : 4 , 'G' : 4 } Tm_simple_params_long = { 'A' : 64.9 , 'T' : 64.9 , 'G' : 64.9 + 41 , 'C' : 64.9 + 41 } if len ( self . seq ) < 14 : return sum ( [ Tm_simple_params_short [ nucleotide ] * self . nucleotide_count ( ) [ nucleotide ] for nucleotide in [ 'A' , 'T' , 'C' , 'G' ] ] ) else : return ( sum ( [ Tm_simple_params_long [ nucleotide ] * self . nucleotide_count ( ) [ nucleotide ] for nucleotide in [ 'A' , 'T' , 'C' , 'G' ] ] ) - 41 * 16.4 ) / len ( self . seq )
short_docstring: Get the Tm using simple calculation.
short_docstring_tokens: Get the Tm using simple calculation .
comment: """
          Get the Tm using simple calculation.
          """