The use of 1,4-butanediol as an intelligent cosubstrate has also been validated in non-aqueous media using a commercial Alcohol dehydrogenase .Kara S , Spickermann D , Weckbecker A , Leggewie C , Arends IWCE , Hollmann F ( 2014 ) Bioreductions Catalyzed by an Alcohol Dehydrogenase in Non-aqueous Media .