the chemiosmotic theory for energy conservation in mitochondria and chloroplasts was proposed by peter mitchell.
the chemiosmotic theory for energy conservation in mitochondria and chloroplasts was proposed by peter mitchell.
the chemiosmotic theory for energy conservation in mitochondria and chloroplasts was proposed by?
peter mitchell
one of moyle's major contributions to the field of biochemistry is her contribution to the development of the chemiosmotic theory. moyle conducted research with peter mitchell on chemiosmotic reactions and reaction mechanisms, which led to the development of the theory in 1967. the chemiosmotic theory explained the mechanism for oxidative phosphorylation, stating that atp synthesis requires chemiosmosis to function. the proton gradient across the inner membrane of the mitochondria is created by the electron transport chain. this causes protons to re-enter the mitochondrial matrix through the protein atp synthase. the movement of protons through this enzyme causes mechanical movement and a conformational change in the enzyme that combines adp and inorganic phosphate to produce atp. the proposal of the chemiosmotic theory was not accepted in the scientific community for over ten years until. when it finally was accepted, peter mitchell received a nobel prize for their work. while the nobel committee recognized moyle's contributions, she did not receive a nobel prize.
jennifer moyle
the chemiosmotic theory was radical at the time, and it was not immediately accepted by the scientific community. one experiment that moyle and mitchell conducted in 1967 was an investigation of ways to improve the precision at which the quotients of translocation of a proton to oxygen can be measured, and what the optimum conditions are for these measurements. their goal was to improve the precision of that measurement in order to allow others to confirm their findings in the proposed chemiosmotic hypothesis. they conducted this experiment because the arrangement of the electron transport chain was only a hypothesis at the time, and it had been inferred from a quotient of protons translocated to oxygen in the chain (→h+/o). moyle and mitchell isolated mitochondria from a rat liver, measured ph displacement across the mitochondrial membrane, and computed the relationship between proton translocation and changes in ph. they found that the pulses of acidification of the inner membrane space of the mitochondria are due to the transport of h+ ions from the mitochondrial matrix into the inner membrane space, and can't be attributed to the formation and breakdown of an intermediate as was previously hypothesized. their work provided sufficient detail for others to reproduce their experiment and confirm their previous findings regarding the chemiosmotic theory.
jennifer moyle