if a solution is blue, yellow - orange will show the most response in terms of absorption.
if a solution is blue, yellow - orange will show the most response in terms of absorption.
if a solution is blue what color will it show the most response in terms of absorption?
yellow - orange
in aqueous solution large numbers of alcian blue molecules stack together as micelles of very large size, too large to be even dialysed. thus even at a fairly high dilution, it has an absorption maximum at ~600–615 nm, which is actually not the absorption maximum of a dye monomer but that of the multimer. since the absorbed light is of yellow orange spectrum, the transmitted/reflected light is perceived by our eye as the complementary color of slightly greenish blue or cyan. in aqueous solution alcian blues continue to be metachromatic at molar concentrations one hundredth those at which toluidine blue is mainly orthochromatic. only a very small shoulder of the absorption curve at 670–680 nm represent the monomeric dye, which is usually the minority and becomes even lesser minority (<10m) in presence of salts. however, when the solvent is dmso—a non-protic solvent of moderately high dielectric constant, alcian blue does not aggregate and a big monomeric absorption peak can be well visualized. a similar spectral shift to the longer monomeric peak is also observed when solvents like ethanol (or ethanol water mixture) is used as a vehicle or when nonionic detergents like triton x-100 are used, that make exogenous micelles.
alcian blue stain
chappuis absorption refers to the absorption of electromagnetic radiation by ozone, which is especially noticeable in the ozone layer, which absorbs a small part of sunlight in the visible part of the electromagnetic spectrum. the chappuis absorption bands occur at wavelengths between 400 and 650 nm. within this range are two absorption maxima of similar height at 575 and 603 nm wavelengths. compared to the absorption of ultraviolet light by the ozone layer, known as the hartley and huggins absorptions, chappuis absorption is distinctly weaker. along with rayleigh scattering, it contributes to the blue color of the sky, and is noticeable when the light has to travel a long path through the atmosphere. for this reason, chappuis absorption only has a significant effect on the color of the sky at dusk, during the so-called blue hour. it is named after the french chemist james chappuis (1854–1934), who discovered this effect.
chappuis absorption