the role of the active site in enzyme catalysed reactions is chemical reaction.
the role of the active site in enzyme catalysed reactions is chemical reaction.
what is the role of the active site in enzyme catalysed reactions?
chemical reaction
the reaction catalysed by an enzyme uses exactly the same reactants and produces exactly the same products as the uncatalysed reaction. like other catalysts, enzymes do not alter the position of equilibrium between substrates and products. however, unlike uncatalysed chemical reactions, enzyme-catalysed reactions display saturation kinetics. for a given enzyme concentration and for relatively low substrate concentrations, the reaction rate increases linearly with substrate concentration; the enzyme molecules are largely free to catalyse the reaction, and increasing substrate concentration means an increasing rate at which the enzyme and substrate molecules encounter one another. however, at relatively high substrate concentrations, the reaction rate asymptotically approaches the theoretical maximum; the enzyme active sites are almost all occupied by substrates resulting in saturation, and the reaction rate is determined by the intrinsic turnover rate of the enzyme. the substrate concentration midway between these two limiting cases is denoted by "k". thus, "k" is the substrate concentration at which the reaction velocity is half of the maximum velocity.
enzyme kinetics
immobilized enzyme









































an immobilized enzyme is an enzyme attached to an inert, insoluble material—such as calcium alginate (produced by reacting a mixture of sodium alginate solution and enzyme solution with calcium chloride). this can provide increased resistance to changes in conditions such as ph or temperature. it also lets enzymes be held in place throughout the reaction, following which they are easily separated from the products and may be used again - a far more efficient process and so is widely used in industry for enzyme catalysed reactions. an alternative to enzyme immobilization is whole cell immobilization.









































section::::commercial use.
immobilized enzyme