low cost is why we use bsa as standard for protein estimation.
low cost is why we use bsa as standard for protein estimation.
why we use bsa as standard for protein estimation?
low cost
bsa has numerous biochemical applications including elisas (enzyme-linked immunosorbent assay), immunoblots, and immunohistochemistry. because bsa is a small, stable, moderately non-reactive protein, it is often used as a blocker in immunohistochemistry. during immunohistochemistry, which is the process that uses antibodies to identify antigens in cells, tissue sections are often incubated with bsa blockers to bind nonspecific binding sites. this binding of bsa to nonspecific binding sites increases the chance that the antibodies will bind only to the antigens of interest. the bsa blocker improves sensitivity by decreasing background noise as the sites are covered with the moderately non-reactive protein. during this process, minimization of nonspecific binding of antibodies is essential in order to acquire the highest signal to noise ratio. bsa is also used as a nutrient in cell and microbial culture. in restriction digests, bsa is used to stabilize some enzymes during the digestion of dna and to prevent adhesion of the enzyme to reaction tubes, pipette tips, and other vessels. this protein does not affect other enzymes that do not need it for stabilization. bsa is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of bsa (see bradford protein assay). bsa is used because of its ability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry. another use for bsa is that it can be used to temporarily isolate substances that are blocking the activity of the enzyme that is needed, thus impeding polymerase chain reaction (pcr).
bovine serum albumin
good examples of this pistol are still available, however many have been poorly maintained and although most parts are still available from specialist suppliers, some are in short supply and restoration is neither straightforward nor cheap.









































a full stocked "carbine" version of the gun, the buccaneer was produced from 1977, neither the scorpion or buccaneer remain in production.









































in the james bond film 'octopussy', octopussy (maud adams) and her team use bsa scorpion pistols loaded with tranquilizers to fight off kamal's men.









































section::::external links.









































bullet::::- bsa website









































bullet::::- screen shot of the original 1970's advertisement
bsa scorpion air pistol