the cell wall is the part of the bacterial cell that is most involved in gram staining.
the cell wall is the part of the bacterial cell that is most involved in gram staining.
3. what part of the bacterial cell is most involved in gram staining?
the cell wall
the cell envelope is composed of the plasma membrane and cell wall. as in other organisms, the bacterial cell wall provides structural integrity to the cell. in prokaryotes, the primary function of the cell wall is to protect the cell from internal turgor pressure caused by the much higher concentrations of proteins and other molecules inside the cell compared to its external environment. the bacterial cell wall differs from that of all other organisms by the presence of peptidoglycan which is located immediately outside of the cytoplasmic membrane. peptidoglycan is made up of a polysaccharide backbone consisting of alternating n-acetylmuramic acid (nam) and n-acetylglucosamine (nag) residues in equal amounts. peptidoglycan is responsible for the rigidity of the bacterial cell wall and for the determination of cell shape. it is relatively porous and is not considered to be a permeability barrier for small substrates. while all bacterial cell walls (with a few exceptions e.g. extracellular parasites such as "mycoplasma") contain peptidoglycan, not all cell walls have the same overall structures. since the cell wall is required for bacterial survival, but is absent in some eukaryotes, several antibiotics (notably the penicillins and cephalosporins) stop bacterial infections by interfering with cell wall synthesis, while having no effects on human cells which have no cell wall, only a cell membrane. there are two main types of bacterial cell walls, those of gram-positive bacteria and those of gram-negative bacteria, which are differentiated by their gram staining characteristics. for both these types of bacteria, particles of approximately 2 nm can pass through the peptidoglycan. if the bacterial cell wall is entirely removed, it is called a protoplast while if it's partially removed, it is called a spheroplast. "β"-lactam antibiotics such as penicillin inhibit the formation of peptidoglycan cross-links in the bacterial cell wall. the enzyme lysozyme, found in human tears, also digests the cell wall of bacteria and is the body's main defense against eye infections.
bacterial cell structure
although bacteria are traditionally divided into two main groups, gram-positive and gram-negative, based on their gram stain retention property, this classification system is ambiguous as it refers to three distinct aspects (staining result, envelope organization, taxonomic group), which do not necessarily coalesce for some bacterial species. the gram-positive and gram-negative staining response is also not a reliable characteristic as these two kinds of bacteria do not form phylogenetic coherent groups. however, although gram staining response is an empirical criterion, its basis lies in the marked differences in the ultrastructure and chemical composition of the bacterial cell wall, marked by the absence or presence of an outer lipid membrane.
gram-positive bacteria