transcription takes place in the nucleus of a typical eukaryotic cell.
transcription takes place in the nucleus of a typical eukaryotic cell.
where does transcription take place in a typical eukaryotic cell?
nucleus
eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in dna into units of transportable complementary rna replica. gene transcription occurs in both eukaryotic and prokaryotic cells. unlike prokaryotic rna polymerase that initiates the transcription of all different types of rna, rna polymerase in eukaryotes (including humans) comes in three variations, each translating a different type of gene. a eukaryotic cell has a nucleus that separates the processes of transcription and translation. eukaryotic transcription occurs within the nucleus where dna is packaged into nucleosomes and higher order chromatin structures. the complexity of the eukaryotic genome necessitates a great variety and complexity of gene expression control.
eukaryotic transcription
these chips haves been developed for ribosomal rna (rrna) targets, commonly used for detecting bacteria. rrna are very abundant in the cell comprising about 80% of the rna content of the typical eukaryotic cell. the rrna is pre-amplified by bacteria and there are present in several thyousand copies per cell, making a good target for microassays. single nucleotide polymorphisms present in the bacterial rrna sequence are used to differentiate bacteria at the genus, species and strain level. this is a unique feature of this microchip that does not require pcr based amplification. the process for detecting bacterial is relatively simple. the bacteria are cultured, washed and pelleted. lysosome is used to lyse the pellets - to destroy the cell walls and release the nucleic acid. lysed bacteria are passed through a colourmn preparation where nucleic acid from the cell is immobilized and other debris is washed out. all the processes after lysis - isolation, purification, fragmentation and labelling of target rrna's - are stable chemical reactions fragments 500 bp easily hybridize to the gel matrix. the total number of eluted off the chip is determined by uv spectrophotometer. the process from sample preparation to identification of organisms based on automated algorithms take place in 2 hours.
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