emulsion test is the method that you use to test for the presence of lipids.
emulsion test is the method that you use to test for the presence of lipids.
what method do you use to test for the presence of lipids?
emulsion test
emulsion test









































the emulsion test is a method to determine the presence of lipids using wet chemistry. the procedure is for the sample to be suspended in ethanol, allowing lipids present to dissolve (lipids are soluble in alcohols). the liquid (alcohol with dissolved fat) is then decanted into water. since lipids do not dissolve in water, when the ethanol is diluted, it falls out of solution to give a cloudy white emulsion.









































add ethanol to the solution you want to test, and add water. a colour change to cloudy white shows that lipids are present.
emulsion test
annular lipids (also called shell lipids or boundary lipids) are a set of lipids or lipidic molecules which preferentially bind or stick to the surface of membrane proteins in biological cells. they constitute a layer, or an annulus/ shell, of lipids which are highly immobilized due to the existence of strong lipid-protein (binding) interactions. polar headgroups of these lipids bind to the hydrophilic part of the membrane protein(s) at the inner and outer surfaces of lipid bilayer membrane. the hydrophobic surface of the membrane proteins is bound to the apposed lipid fatty acid chains of the membrane bilayer. for integral membrane proteins spanning the thickness of the membrane bilayer, these annular/shell lipids act like a lubricating layer on the proteins' surfaces, thereby facilitating almost free rotation and lateral diffusion of membrane proteins within the 2-dimensional expanse of the biological membrane(s). outside the layer of shell/annular lipids, lipids are not tied down to protein molecules. however, they may be slightly restricted in their segmental motion freedom due to mild peer pressure of protein molecules, if present in high concentration, which arises from extended influence of protein-lipid interaction. membrane areas away from protein molecules contain lamellar phase bulk lipids, which are largely free from any restraining effects due to protein-lipid interactions. thermal denaturation of membrane proteins may destroy the secondary and tertiary structure of membrane proteins, exposing newer surfaces to membrane lipids and therefore increasing the number of lipids molecules in the annulus/shell layer. this phenomenon can be studied by the spin label electron paramagnetic resonance technique.
annular lipid shell