dna ligase is the class of the enzymes that can be used to join two fragments of dna.
dna ligase is the class of the enzymes that can be used to join two fragments of dna.
which class of the enzymes can be used to join two fragments of dna?
dna ligase
where the lowercase letters can signify the small, dependent groups. ligase can join two complementary fragments of nucleic acid and repair single stranded breaks that arise in double stranded dna during replication.









































section::::nomenclature.









































the common names of ligases often include the word "ligase", such as dna ligase, an enzyme commonly used in molecular biology laboratories to join together dna fragments. other common names for ligases include the word "synthetase", because they are used to synthesize new molecules.
ligase
denaturing high performance liquid chromatography (dhplc) uses reversed-phase hplc to interrogate snps. the key to dhplc is the solid phase which has differential affinity for single and double-stranded dna. in dhplc, dna fragments are denatured by heating and then allowed to reanneal. the melting temperature of the reannealed dna fragments determines the length of time they are retained in the column. using pcr, two fragments are generated; target dna containing the snp polymorphic site and an allele-specific dna sequence, referred to as the normal dna fragment. this normal fragment is identical to the target dna except potentially at the snp polymorphic site, which is unknown in the target dna. the fragments are denatured and then allowed to gradually reanneal. the reannaled products are added to the dhplc column. if the snp allele in the target dna matches the normal dna fragment, only identical homoduplexes will form during the reannealing step. if the target dna contains a different snp allele than the normal dna fragment, heteroduplexes of the target dna and normal dna containing a mismatched polymorphic site will form in addition to homoduplexes. the mismatched heteroduplexes will have a different melting temperature than the homoduplexes and will not be retained in the column as long. this generates a chromatograph pattern that is distinctive from the pattern that would be generated if the target dna fragment and normal dna fragments were identical. the eluted dna is detected by uv absorption.
snp genotyping