eosin is the secondary stain in the acid fast stain.
eosin is the secondary stain in the acid fast stain.
what is the secondary stain in the acid fast stain?
eosin
Na
Na
section::::staining procedure.









































section::::staining procedure.:make an acid-fast stain.









































section::::staining procedure.:make an acid-fast stain.:materials:.









































bullet::::- slide with organism smear









































bullet::::- carbol fuchsin









































bullet::::- acid-alcohol









































bullet::::- methylene blue









































bullet::::- bibulous paper









































bullet::::- microscope









































section::::staining procedure.:make an acid-fast stain.:instructions:.









































bullet::::1. make smear on a slide with organisms









































bullet::::- clean slide, wax label slide, spread organism, air dry for 10 minutes, heat fix









































bullet::::2. dip slide into carbol fuchsin for 20 minutes.









































bullet::::3. rinse slide









































bullet::::4. dip slide into acid-alcohol for 3-5 seconds.









































bullet::::5. rinse slide









































bullet::::6. dip slide into methylene blue for 30 seconds.









































bullet::::7. rinse slide









































bullet::::8. blot slide dry with bibulous paper.
kinyoun stain