longer dna molecules move more slowly than shorter ones during electrophoresis because of the negative charge.
longer dna molecules move more slowly than shorter ones during electrophoresis because of the negative charge.
why do longer dna molecules move more slowly than shorter ones during electrophoresis?
the negative charge
nucleic acid electrophoresis is an analytical technique used to separate dna or rna fragments by size and reactivity. nucleic acid molecules which are to be analyzed are set upon a viscous medium, the gel, where an electric field induces the nucleic acids (which are negatively charged due to their sugar-phosphate backbone) to migrate toward the anode (which is positively charged because this is an electrolytic rather than galvanic cell). the separation of these fragments is accomplished by exploiting the mobilities with which different sized molecules are able to pass through the gel. longer molecules migrate more slowly because they experience more resistance within the gel. because the size of the molecule affects its mobility, smaller fragments end up nearer to the anode than longer ones in a given period. after some time, the voltage is removed and the fragmentation gradient is analyzed. for larger separations between similar sized fragments, either the voltage or run time can be increased. extended runs across a low voltage gel yield the most accurate resolution. voltage is, however, not the sole factor in determining electrophoresis of nucleic acids.
gel electrophoresis of nucleic acids
in simple terms, electrophoresis is a process which enables the sorting of molecules based on size. using an electric field, molecules (such as dna) can be made to move through a gel made of agarose or polyacrylamide. the electric field consists of a negative charge at one end which pushes the molecules through the gel, and a positive charge at the other end that pulls the molecules through the gel. the molecules being sorted are dispensed into a well in the gel material. the gel is placed in an electrophoresis chamber, which is then connected to a power source. when the electric current is applied, the larger molecules move more slowly through the gel while the smaller molecules move faster. the different sized molecules form distinct bands on the gel.
gel electrophoresis