substrates fit into an enzyme.
substrates fit into an enzyme.
where does a substrate fit into an enzyme?
substrates
in biochemistry, the substrate is a molecule upon which an enzyme acts. enzymes catalyze chemical reactions involving the substrate(s). in the case of a single substrate, the substrate bonds with the enzyme active site, and an enzyme-substrate complex is formed. the substrate is transformed into one or more products, which are then released from the active site. the active site is then free to accept another substrate molecule. in the case of more than one substrate, these may bind in a particular order to the active site, before reacting together to produce products. a substrate is called 'chromogenic' if it gives rise to a coloured product when acted on by an enzyme. in histological enzyme localization studies, the colored product of enzyme action can be viewed under a microscope, in thin sections of biological tissues. similarly, a substrate is called 'fluorogenic' if it gives rise to a fluorescent product when acted on by an enzyme.
substrate (chemistry)
the michaelis constant is equal to the substrate concentration at which the enzyme converts substrates into products at half its maximal rate and hence is related to the affinity of the substrate for the enzyme. the catalytic constant (formula_6) is the rate of product formation when the enzyme is saturated with substrate and therefore reflects the enzyme's maximum rate. the rate of product formation is dependent on both how well the enzyme binds substrate and how fast the enzyme converts substrate into product once substrate is bound. for a kinetically perfect enzyme, every encounter between enzyme and substrate leads to product and hence the reaction velocity is only limited by the rate the enzyme encounters substrate in solution. hence the upper limit for formula_1 is equal to rate of substrate diffusion which is between10 and 10 sm.
specificity constant