blood plasma is the liquid in blood culture bottles.
blood plasma is the liquid in blood culture bottles.
what is the liquid in blood culture bottles?
blood plasma
a minimum of 10 ml of blood is taken through venipuncture and injected into two or more "blood bottles" with specific media for aerobic and anaerobic organisms. a common medium used for anaerobes is thioglycollate broth.









































the blood is collected using aseptic technique. this requires that both the tops of the culture bottles and the venipuncture site of the patient are cleaned prior to collection by swabbing with 70% isopropyl alcohol (povidone and left to dry before venipuncture).
blood culture
isolation and identification is challenging because growth of the bacteria is inhibited by sodium polyanethol sulfonate, an anticoagulant present in most commercial aerobic blood culture bottles that are used as enriched growth media. trypticase soy agar or broth containing 10-20% blood, serum, or ascites fluid, as well as anaerobic culture bottles and resin bead culture systems can be used for growth because sodium polyanethol sulfonate is not normally present. samples from the inner ear of rats have been used to inoculate 5% sheep blood agar, which ranges in ph from 7.2 to 7.6. the samples were incubated at 37 °c, the same as the average temperature of the human body. it typically takes 2 to 3 days for colonies to appear, however it may take as long as 7. colonies appear round, convex, smooth, shiny, and greyish in color on solid agar but, in liquid media, colonies appear as "cotton balls." once they have been cultured, confirmation of their identity can be made using conventional biochemical analyses, such as tests for the production of oxidase, catalase, indole, nitrate, as well as carbohydrate fermentation. "s. moniliformis" can be biochemically differentiated by similar bacteria by their negative production of indole, catalase, and oxidase, and reduction of nitrate to nitrite. "s. moniliformis" has been shown to be sensitive to penicillin, cephalosporins, macrolides, tetracyclines and other broad spectrum antibiotics.
streptobacillus moniliformis