mobile phase is more polar in reverse phase column chromatography.
mobile phase is more polar in reverse phase column chromatography.
in reverse phase column chromatography which phase is more polar?
mobile
chromatography is based on the concept of partition coefficient. any solute partitions between two immiscible solvents. when we make one solvent immobile (by adsorption on a solid support matrix) and another mobile it results in most common applications of chromatography. if the matrix support, or stationary phase, is polar (e.g. paper, silica etc.) it is forward phase chromatography, and if it is non-polar (c-18) it is reverse phase.









































section::::techniques by chromatographic bed shape.









































section::::techniques by chromatographic bed shape.:column chromatography.
chromatography
in chemistry, silica gel is used in chromatography as a stationary phase. in column chromatography, the stationary phase is most often composed of silica gel particles of 40–63 μm. different particle sizes are used for different kinds of column chromatography as the particle size is related to surface area. the differences in particle size dictate if the silica gel should be used for flash or gravity chromatography. in this application, due to silica gel's polarity, non-polar components tend to elute before more polar ones, hence the name normal phase chromatography. however, when hydrophobic groups (such as c groups) are attached to the silica gel then polar components elute first and the method is referred to as reverse phase chromatography. silica gel is also applied to aluminium, glass, or plastic sheets for thin layer chromatography.
silica gel