antibody cocktail to sars-cov-2 spike protein induces rapid mutational escape seen with individual antibodies
antibody cocktail to sars-cov-2 spike protein induces rapid mutational escape seen with individual antibodies
the prospective goal of generating this very large collection was to select pairs of highly potent individual antibodies that could simultaneously bind the rbd spike, and thus might be ideal partners for a therapeutic antibody cocktail that not only could be an effective treatment, but might also protect against antibody resistance resulting from virus escape mutants that could arise in response to selective pressure from single-antibody treatments.", "our data strongly support the notion that cocktail therapy may provide a powerful way to minimize mutational escape by sars-cov-2; in particular, our studies point to the potential value of antibody cocktails in which two antibodies were chosen so as to bind to distinct and non-overlapping regions of the viral target (in this case, the rbd of the spike protein), and thus require the unlikely occurrence of simultaneous mutations at two distinct genetic sites for viral escape.", 


































"to functionally confirm that the spike protein mutations detected by sequencing are responsible for the loss of sars-cov-2 neutralization by the antibodies, we generated vsv-sars-cov-2 spike pseudoparticles expressing the individual identified spike mutations.", "for an antibody cocktail in which the components demonstrate complete competition (regn10989+regn10934), a single amino acid substitution was sufficient to ablate neutralization of the cocktail; hence, both of these antibodies require binding to the glu484 residue in order to neutralize sars-cov-2."


































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