inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °c increases its genome integrity seriously
inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °c increases its genome integrity seriously
our results showed that only 50.11% of the detectable viral templates left after the inactivation of 56 °c for 30 minutes and only 3.36% left after the inactivation of 92 °c for 5 minutes when the samples were preserved by hanks solution, one of an isotonic salt solutions currently suggested.", "according to the laboratory technical guidelines for detection 2019-ncov (fourth edition) issued by national health and commission of china and the experts consensus on nucleic acid detection of 2019-ncov released by chinese society of laboratory medicine, the human samples must be placed under 56°c or higher to inactivate the viruses in order to keep the inspectors from virus infection before the nucleic acids were isolated as the template of qrt-pcr.", 


































"we therefore highly recommend to carry out systematic investigation on the impact of high temperature inactivation on the integrity of 2019-ncov genome and develop a sample preservation solution to protect the detectable templates of 2019-ncov nucleic acids from high temperature inactivation damage.", "however, the detectable templates of viral nucleic acids can be unchanged after the samples were incubated at 56 °c or higher if the samples were preserved with an optimized solution to protect the rna from being disrupted."


































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