a third point mutation in the n gene of sars-cov-2 may affect the detection of the virus by rt-qpcr.
a third point mutation in the n gene of sars-cov-2 may affect the detection of the virus by rt-qpcr.
finally, rt-qpcr primers are designed to amplify the target regions of the sars-cov-2 genome.", "once virus mutation and recombination occur, it is difficult to effectively diagnose viral infection by existing rt-qpcr primers.", "there may be selective mutations in sars-cov-2, and it is necessary to avoid certain regions when designing primers and probes.", "the large- scale sustained person-to-person transmission of sars-cov-2 has led to many mutational events, some of which may affect the sensitivity and specificity of available pcr assays (2).", 


































"a mutation in a reverse primer has been shown to affect the sensitivity of an rt-qpcr assay in use early in the pandemic10 and further mutations could lead to the occurrence of false negative results, particularly if the mutation occurs at the extreme 3 end of the primers.")