 Also to prevent disease onset in greenhouse settings, use UV-absorbing vinyl film, to prevent fungal sporulation [5].Currently cultural practices and fungicides work well in greenhouses and in the field only if "D. bryoniae" is diagnosed in the early stages of disease development [8].Molecular tools such as Polymerase Chain Reaction (PCR), PCR-enzyme-linked immunosorbent assay and magnetic-capture hybridization multiplex real-time PCR are used to diagnose "D. bryoniae" in the early stages disease development, although these molecular tools may only be useful for specific isolates of "D. bryoniae" [8,9,10].