 It was completed around the end of 1485.It includes several symbols of Mary's virginity (the hortus conclusus in the background), purity (white lilies), her sinless state (the white flowers in the 'mystic vase' at the foot of her throne) and her status as a new Venus (myrtle).The red flowers in the vase also allude to Christ's Passion and to the two Johns' martyrdoms, whilst the olive and laurel branches refer to the mystery of the Incarnation.The ascetic character of the figure of Mary shows Savonarola's influence on the artist.