 By 1600, the population of Ghent had shrunk from 50,000 (before the Revolt) to 31,000 inhabitants.With the except of Ostend, the Revolt was over in Flanders, and by the time Maurice of Nassau conducted his campaign towards Dunkirk, leading to the Battle of Nieuwpoort (1600), the Flemish populace did not support him.At the conclusion of the Siege of Ostend in 1604, the emerging Dutch Republic lost its last major Flemish city.