 Anne Sophia died on 3 June 1643 of smallpox.Her husband did not long outlive her; Carnarvon was killed at the first Battle of Newbury on 20 September 1643.Anna Sophia Herbert was born in around 1610, the daughter of Philip Herbert, 4th Earl of Pembroke and Lady Susan de Vere, the youngest daughter of the Elizabethan courtier, poet, and playwright, Edward de Vere, the 17th Earl of Oxford.