 Rags do not contain membrane-targeting sequences, and as a result, depend on the entire Ragulator-Rag Complex to bind to the lysosome, activating mTORC1.While most amino acids indirectly activate mTORC1 in mammals, Leucine has the ability to directly activate mTORC1 in cells that are depleted of amino acids.Yeast contain LRS (leucyltRNA synthetase), which is a molecule that can interact with Rags, directly activating the molecule.