 During his stint at Ruhr University Bochum, Sane worked on the modality of electron transport and transduction of energy in chloroplast membranes and discovered that the generation of pH gradient across the membranes is due to the presence of proton-translocating proteins.Basing his studies on a mutant of Gateway Barley, he did an elaborate biochemical analysis of carbon assimilation and reactions of Ch1 biosynthesis.Associating with Roderic Park, he developed a new method for the isolation of PS-I and PS-II, without the use of detergents and propounded a model for distributing the two photosystems in the chloroplast lamellae.