 This cleavage is highly sensitive to mismatches and can be used to interrogate SNPs with a high degree of specificity

In the basic Invader assay, a FEN called cleavase is combined with two specific oligonucleotide probes, that together with the target DNA, can form a tripartite structure recognized by cleavase.The first probe, called the Invader oligonucleotide is complementary to the 3’ end of the target DNA.The last base of the Invader oligonucleotide is a non-matching base that overlaps the SNP nucleotide in the target DNA.