 The temperature gradient gel electrophoresis (TGGE) or temperature gradient capillary electrophoresis (TGCE) method is based on the principle that partially denatured DNA is more restricted and travels slower in a porous material such as a gel.This property allows for the separation of DNA by melting temperature.To adapt these methods for SNP detection, two fragments are used; the target DNA which contain the SNP polymorphic site being interrogated and an allele-specific DNA sequence, referred to as the normal DNA fragment.