 The TaqMan assay is performed concurrently with a PCR reaction and the results can be read in real-time as the PCR reaction proceeds (McGuigan & Ralston 2002).The assay requires forward and reverse PCR primers that will amplify a region that includes the SNP polymorphic site.Allele discrimination is achieved using FRET combined with one or two allele-specific probes that hybridize to the SNP polymorphic site.