 For example, in the wound defense response, phospholipase C will cause the release of alpha-linolenic acid for JA synthesis.In the first step, alpha-linolenic acid is oxidized by the enzyme lipoxygenase.This forms 13-hydroperoxylinolenic acid, which is then modified by a dehydrase and undergoes cyclization by allene oxide cyclase to form 12-oxo-phytodienoic acid.