 In December 2018, CCMV replication was fully reconstituted in "Saccharomyces cerevisiae", a type of yeast.In this experiment, it was found that protein 1a was the only viral factor needed to induce invagination of the endoplasmic reticulum and begin the replication process.The 2a polymerase was found to be recruited by protein 1a after the formation of the replication spherule.