 Signs of breast development, such as increased nipple sensitivity, were, along with decreased male-pattern hair, the earliest indications of feminization, and occurred in all subjects within 6 weeks.Nilutamide by itself more than doubled luteinizing hormone (LH) and testosterone levels and tripled estradiol levels.The addition of 100 μg/day oral ethinylestradiol to nilutamide therapy after 8 weeks abolished the increase in , testosterone, and estradiol levels and dramatically suppressed testosterone levels, into the female/castrate range.