 If cells are exposed to these enzymes for too long then cell death results, but if they are not exposed to the enzymes for long enough then digestion of the extracellular matrix will not be complete.After the enzymes have been removed from the tissue by perfusing it with a second solution that does not contain enzymes, cells can be mechanically separated or dissociated.A simple technique for dissociating cells involves cutting the tissue into small chunk before agitating the chunks in a solution using a pipette.