 PA 501 begins at an intersection with US 222/PA 272 in the community of Glen Moore in Manheim Township, Lancaster County, north of the city of Lancaster.From here, the route heads north on Lititz Pike, a five-lane road with a center left-turn lane, with the southbound direction running concurrent with southbound US 222.The road passes through business areas and runs to the east of a residential neighborhood before reaching an interchange with the US 30 freeway, where the road is a four-lane divided highway.