 The first section of I-65 to be completed in Indiana was a stretch between a temporary connection with US 52 near Royalton in Boone County (just south of the present-day interchange with I-865) and the US 52 junction northwest of Lebanon, which opened in December 1960.The initial southern Indiana portion, running between then-US 31E (now US 31) in Clarksville and US 50 east of Seymour, saw its first traffic in November 1961.The final of the 17 segments of I-65 outside of I-465, from SR 252 near Edinburgh to Southport Road on Indy's far south side, opened on June 30, 1972.