 Phosphorylation of SIRT6 on S10 facilitates the mobilization of SIRT6 to DNA damage sites, where SIRT6 then recruits and mono-phosphorylates poly (ADP-ribose) polymerase 1 (PARP1) at DNA break sites.Half maximum accumulation of PARP1 occurs within 1.6 seconds after the damage occurs.The chromatin remodeler Alc1 quickly attaches to the product of PARP1 action, a poly-ADP ribose chain, allowing half of the maximum chromatin relaxation, presumably due to action of Alc1, by 10 seconds.