FISSEQ schematic. A tagged random hexamer  primer is used to prime M-MuLV reverse transcriptase to generate aminoallyl dUTP- modified cDNA fragments in fixed cells or tissues.  BS(PEG)9 permanently cross-links the modified cDNA and the cellular protein matrix.  After cDNA circularization, Phi29 DNA polymerase generates cDNA amplicon. The  cross-linked amplicons in the 3D in situ RNA within the cell is sequenced.
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Sinverso