Some bacteria such as enteropathogenic E. coli and enterohemorrhagic E. coli can induce membrane ruffling by secreting toxins via the type three secretion system and modifying the host cytoskeleton. Such toxins include EspT, Map, and SopE, which mimic RhoGEF and activate endogenous Rho GTPases to manipulate actin polymerisation in the infected cell.