The BBE-like enzyme's optimum pH is 8.9, this means it works in an alkaline medium, but the isoelectric point of the homogeneous enzyme is located at pH 4.9, which is a rather an acid medium. This value was obtained with isoelectric focusing and chromatofocusing techniques.
The enzyme broad temperature range is between 40-50 degrees Celsius. The molecular weight of the protein was determined by two processes that show two different results: by SDS gel electrophoresis comes to be 54 kD, and by gel filtration on AcA 54 the enzyme corresponds to a molecular weight of 49 kD, adopting globular shape. A purification study shows that the true molecular weight is in the range of 52 ±4 kD.
On the other hand, total activity decreases drastically during the stationary phase.