Bacterial recombination undergoes various different processes. The processes include: transformation, transduction, conjugation and homologous recombination. Homologous recombination relies on cDNA transferring genetic material. Complementary DNA sequences transport genetic material in the identical homologous chromosomes. The paternal and maternal paired chromosomes will align in order for the DNA sequences to undergo the process of crossing over. Transformation involves the uptake of exogenous DNA from the encircling environment. DNA fragments from a degraded bacterium will transfer into the surrounding, competent bacterium resulting in an exchange of DNA from the recipient. Transduction is associated with viral-mediated vectors transferring DNA material from one bacterium to another within the genome. Bacterial DNA is placed into the bacteriophage genome via bacterial transduction. In bacterial conjugation, DNA is transferred via cell-to-cell communication. Cell-to-cell communication may involve plasmids that allow for the transfer of DNA into another neighboring cell. The neighboring cells absorb the F-plasmid (fertility plasmid: inherited material that is present in the chromosome). The recipient and donor cell come into contact during a F-plasmid transfer. The cells undergo horizontal gene transfer in which the genetic material is transferred.
Mechanisms for double-stranded breaks
The RecBCD pathway in homologous recombination repairs the double-strand breaks in DNA that has degraded in bacteria. Base pairs attached to the DNA strands go through an exchange at a Holliday junction. In the second step of bacterial recombination, branch migration. involves the base pairs of the homologous DNA strands to continuously be interchanged at a Holliday junction. This results in the formation of two DNA duplexes. The RecBCD pathway undergoes helicase activity by unzipping the DNA duplex and stops when the nucleotide sequence reaches 5′-GCTGGTGG-3′. This nucleotide sequence is known as the Chi site. RecBCD enzymes will change after the nucleotide sequence reaches the Chi site. The RecF pathway repairs the degradation of the DNA strands.