There are several bacteria that use the Entner–Doudoroff pathway for metabolism of glucose and are unable to catabolize via glycolysis (e.g., therefore lacking essential glycolytic enzymes such as phosphofructokinase as seen in Pseudomonas).  Genera in which the pathway is prominent include Gram-negative, as listed below, Gram-positive bacteria such as Enterococcus faecalis, as well as several in the Archaea, the second distinct branch of the prokaryotes (and the "third domain of life", after the prokaryotic Eubacteria and the eukaryotes). Due to the low energy yield of the ED pathway, anaerobic bacteria seem to mainly use glycolysis while aerobic and facultative anaerobes are more likely to have the ED pathway. This is thought to be due to the fact that aerobic and facultative anaerobes have other non-glycolytic pathways for creating ATP such as oxidative phosphorylation. Thus, the ED pathway is favored due to the lesser amounts of proteins required. While anaerobic bacteria must rely on the glycolysis pathway to create a greater percentage of their required ATP thus its 2 ATP production is more favored over the ED pathway's 1 ATP production.
Examples of bacteria using the pathway are:
* Pseudomonas, a genus of Gram-negative bacteria
* Azotobacter, a genus of Gram-negative bacteria
* Rhizobium, a plant root-associated and plant differentiation-active genus of Gram-negative bacteria
* Agrobacterium, a plant pathogen (oncogenic) genus of Gram-negative bacteria, also of biotechnologic use
* Escherichia coli, a Gram-negative bacterium
* Enterococcus faecalis, a Gram-positive bacterium
* Zymomonas mobilis, a Gram-negative facultative anaerobe
* Xanthomonas campestris, a Gram-negative bacterium which uses this pathway as main pathway for providing energy.
To date there is evidence of Eukaryotes using the pathway, suggesting it may be more widespread than previously thought: 
*Hordeum vulgare, barley uses the Entner–Duodoroff pathway.
*Phaeodactylum tricornutum diatom model species presents functional phosphogluconate dehydratase and dehoxyphosphogluconate aldolase genes in its genome 
The Entner–Doudoroff pathway is present in many species of Archaea (caveat, see following), whose metabolisms "resemble... in [their] complexity those of Bacteria and lower Eukarya", and often include both this pathway and the Embden-Meyerhof-Parnas pathway of glycolysis, except most often as unique, modified variants.