The serendipity discovery of dye-ligand ability is from a blue dye called blue dextran. The blue dye is used as a void volume (V) marker for a gel filtration column. It has shown that the dye has a property to bind to some certain proteins like pyruvate kinase and elute out with the void volume. Later on, it was found that "cibacron blue FG3-A", reactive dye link to dextran, is responsible for the interaction with the proteins.