Higher selectivity: for example, positive, negative and neutral substances could be separated by a reversed phase (RP)/anion-cation exchange (ACE) column in a single run.
Higher loading capacity,
for example, loading capacity of ACE/ hydrophilic interaction chromatography (HILIC) increased 10-100 times when compared with RPLC,
which offered a new selection and idea for developing semi-preparative and preparative chromatography.
One mixed-mode column can replace two or even more single mode columns, which is economic and eco-friendly for employing the stationary phase more sufficiently and reducing the consuming and ‘waste’ of raw materials.
Single mixed-mode column can be applied for on-line two-dimensional (2D) analysis in a sealed system via establishing corresponding chromatographic system or off-line 2D analysis as two columns.