Using the MagPen (a Nano3D Biosciences, Inc. product), organized 3D co-cultures similar to native tissue architecture can be rapidly created. Endothelial cells (PEC), smooth muscle cells (SMC), fibroblasts (PF), and epithelial cells (EpiC) cultured with the Bio-Assembler can be sequentially layered in a drag-and-drop manner to create bronchioles that maintain phenotype and induce extracellular matrix formation.