After time is allowed for binding with the oligonucleotide probes, the hope is that some of the proteins on the membrane filter have bound to the probes. Any probe that was not able to bind a protein needs to be removed. Once unbound probe removal has been taken care of, to better visualize the membrane filter, it is subjected to further varying procedures. By corresponding the resulting membrane filter to the second membrane filter that the gel was sandwiched between, the position of the protein in comparison to the molecular weight ladder gives information about the weight of the protein that bound to the probe.