In interfacial synthesis, the polymerization happens at the interface between an aqueous and an organic layer. A typical reaction involves an aqueous solution of acid and oxidant and an organic layer of aniline together. This creates the reactive interface for polymerization to occur. As polymerization proceeds, the polyaniline nanofibers will diffuse into the water layer, leaving the reactive interface. This prevents overgrowth onto the existing wires, allowing for homogeneous nucleation to continue occurring. Conditions in the interfacial synthesis can be tuned, such as the type of acid used as well as the oxidant used.