To date, PAMPA models have been developed that exhibit a high degree of correlation with permeation across a variety of barriers, including Caco-2 cultures, the gastrointestinal tract, blood–brain barrier and skin.
The donor and/or acceptor compartments may contain solubilizing agents, or additives that bind the drugs as they permeate.  To improve the in vitro - in vivo correlation and performance of the PAMPA method, the lipid, pH and chemical composition of the system is often designed with biomimetic considerations in mind.
Although active transport is not modeled by the artificial PAMPA membrane, up to 95% of known drugs are absorbed by passive transport. Some experts support a lower figure, so the amount is open to some interpretation.  Microtiter plates with 96 wells can be used for the assay which increases the speed and lowers the per sample cost.