Radionuclides are used in two major ways: either for their radiation alone (irradiation, nuclear batteries) or for the combination of chemical properties and their radiation (tracers, biopharmaceuticals).
* In biology, radionuclides of carbon can serve as radioactive tracers because they are chemically very similar to the nonradioactive nuclides, so most chemical, biological, and ecological processes treat them in a nearly identical way. One can then examine the result with a radiation detector, such as a Geiger counter, to determine where the provided atoms were incorporated. For example, one might culture plants in an environment in which the carbon dioxide contained radioactive carbon; then the parts of the plant that incorporate atmospheric carbon would be radioactive. Radionuclides can be used to monitor processes such as DNA replication or amino acid transport.
* in physics and biology radionuclide X-ray fluorescence spectrometry is used to determine chemical composition of the compound. Radiation from a radionuclide source hits the sample and excites characteristic X-rays in the sample. This radiation is registered and the chemical composition of the sample can be determined from the analysis of the measured spectrum. By measuring the energy of the characteristic radiation lines, it is possible to determine the proton number of the chemical element that emits the radiation, and by measuring the number of emitted photons, it is possible to determine the concentration of individual chemical elements.
* In nuclear medicine, radioisotopes are used for diagnosis, treatment, and research. Radioactive chemical tracers emitting gamma rays or positrons can provide diagnostic information about internal anatomy and the functioning of specific organs, including the human brain. This is used in some forms of tomography: single-photon emission computed tomography and positron emission tomography (PET) scanning and Cherenkov luminescence imaging. Radioisotopes are also a method of treatment in hemopoietic forms of tumors; the success for treatment of solid tumors has been limited. More powerful gamma sources sterilise syringes and other medical equipment.
* In food preservation, radiation is used to stop the sprouting of root crops after harvesting, to kill parasites and pests, and to control the ripening of stored fruit and vegetables. Food irradiation usually uses beta-decaying nuclides with strong gamma emissions like cobalt-60 or caesium-137.
* In industry, and in mining, radionuclides are used to examine welds, to detect leaks, to study the rate of wear, erosion and corrosion of metals, and for on-stream analysis of a wide range of minerals and fuels.
* In spacecraft, radionuclides are used to provide power and heat, notably through radioisotope thermoelectric generators (RTGs) and radioisotope heater units (RHUs).
* In astronomy and cosmology, radionuclides play a role in understanding stellar and planetary process.
* In particle physics, radionuclides help discover new physics (physics beyond the Standard Model) by measuring the energy and momentum of their beta decay products (for example, neutrinoless double beta decay and the search for weakly interacting massive particles).
* In ecology, radionuclides are used to trace and analyze pollutants, to study the movement of surface water, and to measure water runoffs from rain and snow, as well as the flow rates of streams and rivers.
* In geology, archaeology, and paleontology, natural radionuclides are used to measure ages of rocks, minerals, and fossil materials.