The bioinformatics core contributes bioinformatic analysis by collecting and curating complete sequence data sets, generating sequence similarity networks, and classification of superfamily members into subgroups and families for subsequent annotation transfer and evaluation as targets for functional characterization.
The protein core develops cloning, expression, and protein purification strategies for the enzymes targeted for study.
The structure core fulfills the structural biology component for EFI by providing high resolution structures of targeted enzymes.
The computation core performs in silico docking to generate rank-ordered lists of predicted substrates for targeted enzymes using both experimentally determined and/or homology modeled protein structures.
The microbiology core examines in vivo functions using genetic techniques and metabolomics to complement in vitro functions determined by the Bridging Projects.
The data and dissemination core maintains a public database for experimental data (EFI-DB).