After the annexation of the Republic of Genoa to the Kingdom of Sardinia at the beginning of the 19th century, the pharmacy was further refurbished by brother Bernardino di Sant'Anna.
In those years, the Pharmacy started a cooperation with the famous Parisian surgeon Louis Le Roy, author of the treatise Healing Medicine, aka Purgation, translated in Italian for the first time in Bologna in 1824. Although its content was later superseded by improvement in scientific research, the treatise enjoyed a great success at the time and was reprinted several times. A Genoese reprint in 1885 mentioned the cooperation with SantAnnas pharmacy in the back cover.
A document stored in the library of the Convent shows that brother Modesto, whose lay name was Stefano Montabone, received his diploma of chemist in 1840, granting him permission to practice his profession in Genoa and all other territories of the Kingdom of Sardinia in accordance to letter patents issued by King Victor Emmanuel I of Savoy. In 1882, however, the difficulties in securing chemist friars with appropriate legal qualifications appeared insurmountable. Therefore, keen to remain licensed according to the law, the Convent of SantAnna decided to enact a compromise solution and appointed a lay chemist "to ensure the regular preparation and administration of the medicines'", while the business administration of the pharmacy remained entrusted to a friar.
At the end of the 19th century, the pharmacy had several prominent clients, including the main hospital of Genoa, the hospital of Pammatone. At the time, the most sought after products were white sugar, quinine, English salts, cinnamomum, rosolio, poppyseed oil, medicines against parasites and a medicine against demodicosis.