Two alternate techniques have been described that allow for 5’ tag analysis in organisms that do not have trans-spliced leader sequences. The techniques presented by Toshiyuki et al. and Shin-ichi et al. are called CAGE and 5’ SAGE respectively. CAGE utilizes biotinylated cap-trapper technology to maintain mRNA signal long enough to create and select full length cDNAs, which have adapter sequences ligated on the 5‘ end. 5’ SAGE utilizes oligo-capping technology. Both use their adapter sequence to prime from after the cDNA is created. Both of these methods have disadvantages though. CAGE has shown tags with addition of a guanine on the first position and oligo-capping may lead to sequence bias due to the use of RNA ligase.