Adolf Hitler was sworn in as the Chancellor of Germany on 30 January 1933, as his Nazi Party (NSDAP) was now the largest party in the Reichstag (Weimar Republic). The 7 April 1933 Law for the Restoration of the Professional Civil Service removed Jews from the civil service, which included academia. Meitner never tried to conceal her Jewish descent, but initially was exempt from its impact on multiple grounds: she had been employed before 1914, had served in the military during the World War, was an Austrian rather than a German citizen, and the Kaiser Wilhelm Institute was a government-industry partnership. However, she was dismissed from her adjunct professorship on 6 September on the grounds that her World War I service was not at the front, and she had not completed her habilitation until 1922. This had no effect on her salary or work at the KWI for Chemistry. Carl Bosch, the director of IG Farben, a major sponsor of the KWI for Chemistry, assured Meitner that her position there was safe. Although Hahn and Meitner remained in charge, their assistants, Otto Erbacher and Kurt Philipp respectively, who were both NSDAP members, were given increasing influence over the day-to-day running of the institute.
Others were not so fortunate; her nephew Otto Frisch was dismissed from his post in the Institute for Physical Chemistry at the University of Hamburg, as was Otto Stern, the director of the institute. Stern found Frisch a position with Patrick Blackett at Birkbeck College in England, and he later worked at the Niels Bohr Institute in Copenhagen from 1934 to 1939. Fritz Strassman had come to the Kaiser Wilhelm Institute for Chemistry to study under Hahn to improve his employment prospects. He declined a lucrative offer of employment because it required political training and Nazi Party membership, and resigned from the Society of German Chemists when it became part of the Nazi German Labour Front rather than become a member of a Nazi-controlled organisation. As a result, he could neither work in the chemical industry nor receive his habilitation. Meitner persuaded Hahn to hire him as an assistant. Soon he would be credited as a third collaborator on the papers they produced, and would sometimes even be listed first. Between 1933 and 1935, Meitner published exclusively in Naturwissenschaften, as its editor Arnold Berliner was Jewish, and continued to accept submissions from Jewish scientists. This generated a boycott of the publication, and in August 1935 publisher Springer-Verlag fired Berliner.