* Instead of relying on standard diffusion to transfer the proteins from the gel to the filter, electroblotting is commonly used because it removes the denaturing agent SDS thereby allowing the proteins to renature as they move to the filter.  
* Skim milk is added to the filter before hybridizing with probes as it contains Bovine Serum Albumin (BSA) which prevents any unwanted or weak interactions of DNA to the nitrocellulose membrane.
* A rapid dimethylsulphate (DMS) protection assay can be used to identify non-specific binding vs. specific binding on blots.
* During the DNA probe hybridization step, defined amounts of salt are used to enhance specific interactions that occur between the DNA and proteins.