Samples that change their properties after dilution may not be analyzed via static light scattering in terms of the simple model presented here as the Zimm equation. A more sophisticated analysis known as composition-gradient static (or multi-angle) light scattering (CG-SLS or CG-MALS) is an important class of methods to investigate protein–protein interactions, colligative properties, and other macromolecular interactions as it yields, in addition to size and molecular weight, information on the affinity and stoichiometry of molecular complexes formed by one or more associating macromolecular/biomolecular species. In particular, static light scattering from a dilution series may be analyzed to quantify self-association, reversible oligomerization, and non-specific attraction or repulsion, while static light scattering from mixtures of species may be analyzed to quantify hetero-association.