Amine oxides with an average chain length of 12.6 have been measured to be water-soluble at ~410 g/L. They are considered to have low bioaccumulation potential in aquatic species based on log K data from chain lengths less than C14 (bioconcentration factor < 87%). Levels of AO in untreated influent were found to be 2.3–27.8 ug/L, while in effluent they were found to be 0.4–2.91 ug/L. The highest effluent concentrations were found in oxidation ditch and trickling filter treatment plants. On average, over 96% removal has been found with secondary activated sludge treatment. Acute toxicity in fish, as indicated by 96h LC50 tests, is in the range of 1,000–3,000 ug/L for carbon chain lengths less than C14. LC50 values for chain lengths greater than C14 range from 600 to 1400 ug/L. Chronic toxicity data for fish is 420 ug/L. When normalized to C12.9, the NOEC is 310 ug/L for growth and hatchability.