* This is an irreversible physical immobilization technique which can be considered as a physical restriction of enzyme in a specified area/space. It can be used for raising mechanical stability and can be also used for the reduction of leaching events of enzymes. Since the enzyme in this process does not interact chemically with the polymer/ material of the support fibers/lattice, it remains protected from denaturation with time.
* Basically, the enzyme is trapped in insoluble beads or microspheres, such as calcium alginate beads. However, these insoluble substances hinder the arrival of the substrate, and the exit of products.