Freeze-dried samples of Caldora penicillata had EtOAc−MeOH and HO−EtOH applied to them in order to extract Caldoramide. The extracts were partitioned with n-BuOH and HO and then fractions were taken based on solubility in either EtOAc or BuOH. Caldoramide was extracted from the BuOH soluble fraction.