Biochemical oxygen demand (also known as BOD or biological oxygen demand) is an analytical parameter representing the amount of dissolved oxygen (DO) consumed by aerobic bacteria growing on the organic material present in a water sample at a specific temperature over a specific time period. The BOD value is most commonly expressed in milligrams of oxygen consumed per liter of sample during 5 days of incubation at 20 °C and is often used as a surrogate of the degree of organic water pollution.
Biochemical Oxygen Demand (BOD) reduction is used as a gauge of the effectiveness of wastewater treatment plants. BOD of wastewater effluents is used to indicate the short-term impact on the oxygen levels of the receiving water.
BOD analysis is similar in function to chemical oxygen demand (COD) analysis, in that both measure the amount of organic compounds in water. However, COD analysis is less specific, since it measures everything that can be chemically oxidized, rather than just levels of biologically oxidized organic matter.