Uniporters play an essential role in carrying out various cellular functions. Each uniporter is specialized to facilitate the transport of a specific molecule or ion across the cell membrane. Examples of a few of the physiological roles uniporters aid in include:
# Nutrient Uptake: Uniporters facilitate the transport of essential nutrients into the cell. Glucose transporters (GLUTs) are uniporters that uptake glucose for energy production.
# Ion homeostasis: Uniporters facilitate in maintaining the balance of ions (i.e., Na+, K+, Ca2+, Cl-)  within cells 
# Metabolism: Uniporters are involved in the transport of essential ions, amino acids and molecules required for the metabolic pathway, protein synthesis and energy production
# Cell signaling: Calcium uniporters help regulate intercellular calcium levels essential for signal transduction
# Waste removal: Uniporters aid in removing metabolic waste products and toxins from cells
# pH regulation: Transport of ions by uniporters also helps to maintain the overall acid-base balance within cells