Unlike in marine sediments, diagenesis does not pose a large threat to the integrity of the C/N ratio in lacustrine sediments. Though wood from living trees around lakes have consistently higher C/N ratios than wood buried in sediment, the change in elemental composition is not large enough to remove the vascular versus non-vascular plant signals due to the refractory nature of terrestrial organic matter. Abrupt shifts in the C/N ratio down-core can be interpreted as shifts in the organic source material.
For example, two separate studies on Mangrove Lake, Bermuda and Lake Yunoko, Japan show irregular, abrupt fluctuations between C/N around 11 to around 18. These fluctuations are attributed to shifts from mainly algal dominance to land-based vascular dominance. Results of studies that show abrupt shifts in algal dominance and vascular dominance often lead to conclusions about the state of the lake during these distinct periods of isotopic signatures. Times in which lakes are dominated by algal signals suggest the lake is a deep-water lake, while times in which lakes are dominated by vascular plant signals suggest the lake is shallow, dry, or marshy. Using the C/N ratio in conjunction with other sediment observations, such as physical variations, D/H isotopic analyses of fatty acids and alkanes, and δ13C analyses on similar biomarkers can lead to further regional climate interpretations that describe the larger phenomena at play.