Sophorolipids are glycolipids consisting of a hydrophobic fatty acid tail of 16 or 18 carbon atoms and a hydrophilic carbohydrate head sophorose, a glucose-derived di-saccharide with an unusual β-1,2 bond and can be acetylated on the 6′- and/or 6′′- positions. One terminal or sub terminal hydroxylated fatty acid is β-glycosidically linked to the sophorose module. The carboxylic end of this fatty acid is either free (acidic or open form) or internally esterified at the 4′′ or in some rare cases at the 6′- or 6′′-position (lactonic form). The physicochemical and biological properties of sophorolipids are significantly influenced by the distribution of the lactone vs. acidic forms produced in the fermentative broth. In general, lactone sophorolipids are more efficient in reducing surface tension and are better antimicrobial agents, whereas acidic sophorolipids display better foaming properties. Acetyl groups can also lower the hydrophilicity of sophorolipids and enhance their antiviral and cytokine stimulating effects.
Sophorolipids are produced by various non pathogenic yeast species such as Candida apicola, Rhodotorula bogoriensis, Wickerhamiella domercqiae, and Starmerella bombicola. Recent research has meant sophorolipids can be recovered during a fermentation using a gravity separator in a loop with the bioreactor, enabling the production of >770 g/L sophorolipid at a productivity 4.24 g/L/h, some of the highest values seen in a fermentation process  Desirable properties of biosurfactants are biodegradability and low toxicity. Sophorolipids produced by several yeasts belonging to candida and the starmerella clade, and Rhamnolipid produced by Pseudomonas aeruginosa etc.
Besides biodegradability, low toxicity, and high production potential, sophorolipids have a high surface and interfacial activity. Sophorolipids are reported to lower surface tension (ST) of water from 72 to 30-35 mN/m and the interfacial tension (IT) water/hexadecane from 40 to 1 mN/m.  In addition to this, sophorolipids are reported to function under wide ranges of temperatures, pressures and ionic strengths; and they also possess a number of other useful biological activities including Antimicrobial, virucidal, Anticancer, Immuno-modulatory properties.