# Clone the P element into a plasmid and transform and grow this in bacteria.
# Eliminate the P transposase and replace it with your gene of interest.
#Microinject the posterior end of an early-stage (pre-cellularization) embryo with DNA coding for transposase and a plasmid with the reporter gene, gene of interest and transposase recognition sequences.
#Random transposition occurs, inserting the gene of interest and reporter gene.
# Once the gene of interest has been inserted it is no longer mobile because it cannot produce its own P transposase.
#Grow flies and cross to remove genetic variation between the cells of the organism. (Only some of the cells of the organism will have been transformed. Hopefully, some of these transformed cells end up in the germ line. A transformed gamete will give rise to an organism with no variation between its cells).
#Look for flies expressing the reporter gene. These carry the inserted gene of interest, so can be investigated to determine the phenotype due to the gene of interest.
The inserted gene may have damaged the function of one of the host's genes. Several lines of flies are required so comparison can take place and ensure that no additional genes have been knocked out.