The main processes involved in secondary sewage treatment are designed to remove as much of the solid material as possible. They use biological processes to digest and remove the remaining soluble material, especially the organic fraction. This can be done with either suspended-growth or biofilm processes. The microorganisms that feed on the organic matter present in the sewage grow and multiply, constituting the biological solids, or biomass. These grow and group together in the form of flocs or biofilms and, in some specific processes, as granules. The biological floc or biofilm and remaining fine solids form a sludge which can be settled and separated. After separation, a liquid remains that is almost free of solids, and with a greatly reduced concentration of pollutants.
Secondary treatment can reduce organic matter (measured as biological oxygen demand) from sewage,  using aerobic or anaerobic processes. The organisms involved in these processes are sensitive to the presence of toxic materials, although these are not expected to be present at high concentrations in typical municipal sewage.