Advantages of HPTLC:
* Provides straightforward information about effects arising from individual compounds in complex or natural samples separated in parallel.
* Combines chromatographic separation with effect-directed detection using enzymatic or biological assays.
* Helps to select important compounds from a sample for further characterization using high-resolution mass spectrometry.
* Offers unique benefits such as super-hyphenation, minimum sample preparation requirements, detection of multi-modulating compounds, and distinguishing agonistic versus antagonistic effects.