Flexibility
Your DNA sequence of interest can be moved across any expression system in just one recombination step when you create the entry clone with it.
Speed
Instead of taking two or more days with conventional restriction and ligation cloning, the Gateway approach allows for the creation of the expression construct in just one day. The attB-PCR products can also be immediately cloned into the target vectors by performing the BP and LR reactions in the same tube. There are no procedures for restriction, ligation, or gel purification during the cloning process.
Multiple fragment cloning
Gateway cloning can be used to simultaneously insert several DNA pieces into numerous vectors in a single tube. To create the necessary expression clone, up to four DNA segments can be cloned into a single Gateway vector in a precise order and orientation in a single tube. The design of the Gateway vectors makes this possible. 
High efficiency
The Gateway Cloning Method uses positive and negative selection markers to increase the chance of successfully cloning a gene. This means that the process is more efficient, meaning it is more likely to produce successful results.
Universality
All types of DNA fragments can be cloned using PCR techniques. Cloning is available for many different kinds of organisms, from mammals to bacteria.