Source:
When hemoglobin is exposed to certain conditions, reversible or irreversible hemichromes are formed.
Reversible hemichrome formation occurs in the presence of:
* Fatty acids
* Aliphatic alcohol (n-butanol)
* Dehydration
* High concentration of glycerol
* Polyethylene glycol
Irreversible hemichrome formation occurs in the presence of:
* Phenylhydrazine
* Sodium dodecyl sulphate (SDS)