Biocurious planned to tweak the biobrick containing six genes, including luciferin-regenerating enzyme and luciferase from fireflies. During initial development, they would use Agrobacterium to test the transfer of the genetic circuit. When producing the final product, they intended to instead use a gene gun to avoid issues related to regulation of GM plants. Over the course of the project, several plants were mentioned as being recipients, including Arabidopsis thaliana, Nicotiana tabacum, and roses. Issues surrounding the production included the difficulty of moving the six component genes of the metabolic pathway, increasing the dim light produced by the plant following insertion, and preventing the pathway from being silenced.