C. albicans is a fungal pathogen of humans and is responsible for hospital-acquired bloodstream infections. MLST technique has used to characterize C. albicans isolates. Combination of the alleles at the different loci results in unique diploid sequence types that can be used to discriminate strains. MLST has been shown successfully applied to study the epidemiology of C. albicans in the hospital as well as the diversity of C. albicans isolates obtained from diverse ecological niches including human and animal hosts.