A lot of research has been conducted on Vectorette PCR and the applications it has in the field of biology. Scientists used Vectorette PCR to take the transgene flanking DNA and isolate it. They used this technique on the DNA belonging to mice that was next to transgene sections. From this the scientists were able to show that the use of Vectorettes is capable of facilitating the recovery and mapping of sequences in complex genomes. They have also found that Vectorette PCR can help in the analysis of sequences by subvectoretting when PCR products of a large size are the subject at hand.
Other work has looked at developing a method using Vectorette PCR in order to accomplish genomic walking. By using Vectorette PCR, scientists were able to acquire single-stranded DNA which were obtained from PCR products in order to sequence them. From this an approach was identified in which the amplification of sequences which were previously uncharacterized was possible. This research demonstrates how novel sequences can be rapidly developed when only a known sequence of DNA is used to start.
Further research has experimented with the creation of a method that progresses the isolation of microsatellite repeats. By using Vectorette PCR, researchers have found a rapid technique to accomplish this with novel, microsatellite repeats. They have attempted and succeeded in using this technique to isolate an amount of six microsatellite repeats.
Vectorette PCR has also been used to not only identify genomic positions of insertion sequences (IS) but also to map them. Research on this has shed light on a way to complete the typing of microbial stains and the identification and mapping of things like IS insertion sites that reside in microbial genomes. Vectorette PCR proves useful when it comes to rapidly and simply surveying genomes’ IS elements.
Transposable element, transposon, or TE is a variation of genetic elements that is capable of changing its location in a genome by a process called “jumping”. TE display is designed to present the different variations of TE insertion sites which helps to make numerous dominant markers. A problem that arose in the original method was finding a PCR method that was capable of being specific and efficient in its output of the transposon within the genome. Researchers have found a solution for this problem by using Vectorette PCR as the PCR method. Since Vectorette PCR is capable of being specific with its isolation and amplification of genes, this helped with their research and aided in improving the method of TE display by saving both time and costs. The researchers were then able to produce numerous dominant markers with the use of Vectorette PCR that is based on a TE display that is nonradioactive.
Thyroid lymphoma is an illness which leads to the transformation of the lymphocytes belonging to the thyroid into cells of a cancerous nature. Researchers have tested a new method that aids in the diagnosis of this condition. The use of Vectorette PCR was combined with restriction enzyme digestion, and it was found that Vectorette PCR proved to be useful in their study and aided in the diagnosis of thyroid lymphoma.
Researchers have looked into the potential use of Vectorette PCR in the examination of the genes of diseases. They have taken two methods, trinucleotide repeats which are specifically used for the targeting of transcribed regions and Vectorette PCR, to obtain simple sequence repeats or SSRs. It is believed that genetic markers can be made from these SSRs. The outcome from this research is hoped to aid researchers attempt the derivation of genetic markers which are transportable from unknown genomes. Vectorette PCR was used to uncover SSRs which flank the trinucleotide repeat that was targeted for testing. This is also known as TNR or trinucleotide Vectorette PCR. They believe that their TNR method combined with the amplification provided by Vectorette PCR can be used in eukaryotes to create molecular markers that are based on simple repeat sequences. The researchers also think that this method will be of value when attempting to isolate genes that are able to bring about diseases.