It was first developed and utilized by Roy Britten and his colleagues at the Carnegie Institution of Washington in the 1960s.  Of particular note, it was through Ct analysis that the redundant (repetitive) nature of eukaryotic genomes was first discovered.  However, it wasn't until the breakthrough DNA reassociation kinetics experiments of Britten and his colleagues that it was shown that not all DNA coded for genes. In fact, their experiments demonstrated that the majority of eukaryotic genomic DNA is composed of repetitive, non-coding elements.