Although initially used in conjunction with UV/Vis spectroscopy, many modifications have been made that allow the B–H method to be applied to other spectroscopic techniques involving fluorescence, infrared, and NMR.
Modifications have also been done to further improve the accuracy in the determination of K and ε based on the Benesi–Hildebrand equations. One such modification was done by Rose and Drago. The equation that they developed is as follows:
Their method relied on a set of chosen values of ε and the collection of absorbance data and initial concentrations of the host and guest. This would thus allow the calculation of K. By plotting a graph of ε versus K, the result would be a linear relationship. When the procedure is repeated for a series of concentrations and plotted on the same graph, the lines intersect at a point giving the optimum value of ε and K. However, some problems have surfaced with this modified method as some examples displayed an imprecise point of intersection or no intersection at all.
More recently, another graphical procedure has been developed in order to evaluate K and ε independently of each other. This approach relies on a more complex mathematical rearrangement of the Benesi–Hildebrand method but has proven to be quite accurate when compared to standard values.