Other methods mapping and profiling the methylome have been effective but are not without their limitations that can affect resolution, level of throughput, or experimental variations.  For instance, RLGS is limited by the number of restriction sites in genome that can be targets for the restriction enzyme; typically, a maximum of ~4100 landmarks can be assessed. Bisulfite sequencing-based methods, despite possible single-nucleotide resolution, have a drawback: the conversion of unmethylated cytosine to uracil can be unstable.  In addition, when bisulfite conversion is coupled with DNA microarrays to detect bisulfite converted sites, the reduced sequence complexity of DNA is a problem.  Microarrays capable of comprehensively profiling the whole-genome become difficult to design as fewer unique probes are available.