The uses that have been derived from Vectorette PCR are many and have been useful to the science of biology. For example, it gives rise to methods that can help during the outbreaks of diseases by making it easier to subtype pathogens that are similar or closely related. It can also be used to help diagnose certain diseases. Earlier in this page it was noted that Vectorette PCR can give rise to multiple functions that can be performed on novel DNA sequences located near a sequence that is already known. These functions like isolating DNA, amplifying it, and analyzing it are behind the uses for Vectorette PCR. These uses are things like genome walking, DNA sequencing for the termini of Yeast Artificial Chromosomes (YAC) and cosmid inserts, being able to map introns and promoters in genomic DNA and regions with mutations, facilitating the sequencing of clones of a large size, and filling in the gaps that arise during the mapping of genomes.
An intron is a DNA sequence that is flanked by exons and therefore located in between them. It is the region that gets cut out while exons are expressed, and so introns do not affect the code of amino acids. Gene expression can be affected by only a number of intronic sequences. Vectorette PCR has been found to be beneficial when it comes to the characterization of these intronic sequences when they are found to be next to known sequences.
cDNA or complementary DNA is a DNA sequence which is complementary to the RNA that is the template when synthesizing DNA during the reverse transcriptase process. Vectorette PCR that utilizes the primers that originate from cDNA gives rise to a method that is capable of acquiring intron sequences which are located adjacent to exons and aiding in the development of the structure of genes. It is able to achieve this when initializing the process with a sequence of cDNA and a clone of a genome.
Vectorette PCR also gives the user an advantage than if he/she were using other existing technologies. The user will be able to carry out tasks like gene manipulation that is cell-free, Vectorette PCR with minimal material to start with, and performing Vectorette PCR with DNA that needs not be of high purity. These advantages allow the user to save time and resources while increasing the range of DNA that can be targeted.