#Isolate the fly genome.
#Undergo a light digest (using an enzyme [enzyme 1] known to cut in the boundary between the reporter gene and the E. coli reporter gene and plasmid sequences), giving fragments of a few kilobases, a few with the E. coli reporter, the plasmid sequences and its flanking DNA.
#Self ligate the digest (low DNA concentration to ensure self ligation) giving a selection of circular DNA fragments, a few with the E. coli reporter, the plasmid sequences and its flanking DNA.
#Insert the plasmids into E. coli cells (e.g. by electroporation).
#Select plasmids for the E. coli selectable marker gene. Only successful inserts of plasmids with the plasmid housekeeping sequences will express this gene.
#The gene can be cloned for further analysis.