Bioaccumulation should be considered during the utilization of the triad approach depending on the study goals. It preparation for measuring bioaccumulation, it must be specified if the test will serve to assess secondary poisoning or biomagnification (Chapman, 1997). Bioaccumulation analysis should be conducted appropriately based on the contaminants of concern (for example, metals do not biomagnify). This can be done with field-collected, caged organisms, or laboratory exposed organisms (Chapman, 1997). While the bioaccumulation portion is recommended, it is not required. However, it serves an important role with the purpose of quantifying effects due to trophic transfer of contaminants through consumption of contaminated prey.