Hans Gross applied scientific methods to crime scenes and was responsible for the birth of criminalistics.
Edmond Locard expanded on Gross work with Locards Exchange Principle which stated "whenever two objects come into contact with one another, materials are exchanged between them". This means that every contact by a criminal leaves a trace.
Alexander Lacassagne, who taught Locard, produced autopsy standards on actual forensic cases.
Alphonse Bertillon was a French criminologist and founder of Anthropometry (scientific study of measurements and proportions of the human body). He used anthropometry for identification, stating that, since each individual is unique, by measuring aspects of physical difference there could be a personal identification system. He created the Bertillon System around 1879, a way of identifying criminals and citizens by measuring 20 parts of the body. In 1884, over 240 repeat offenders were caught using the Bertillon system, but the system was largely superseded by fingerprinting.
Frances Glessner Lee, known as "the mother of forensic science", was instrumental in the development of forensic science in the US. She lobbied to have coroners replaced by medical professionals, endowed the Harvard Associates in Police Science, and conducted many seminars to educate homicide investigators. She also created the Nutshell Studies of Unexplained Death, intricate crime scene dioramas used to train investigators, which are still in use today.