Systemic ablation of dendritic cells (DCs) was shown to cause fatal manifestations of autoimmunity which points to their importance in central tolerance. Indeed, as mTECs represent exclusive donors of TRAs, experiments with first antigen transfer mouse models discovered thymic dendritic cells (DCs) to be so far the only known TRAs acceptors involved in antigen transfer. Indispensability of DCs for the establishment of central tolerance was further verified by recent analysis, which revealed that DCs mediate both recessive and dominant tolerance, with preference for the latter, via presentation of more common TRAs.