Design and fabrication of E-AB aptamers is consistent with methods used for previously reported aptamers. SELEX is a well known selection method for fabrication and selection of nucleotide aptamers. In 1990s, scientists introduced SELEX. Aptamers are chosen based on their in vitro target recognition through this process. In SELEX, aptamers are chosen based on their ability to recognize specific targets. This method involves three key steps: First, single-stranded nucleic acids are bound to the target. Next, the bound nucleic acids are separated from unbound ones. Finally, Polymerase Chain Reaction (PCR) amplifies the nucleic acids that have an affinity for the target, allowing for further screening or functional analysis. Following SELEX, high-throughput sequencing is used to identify sequences that have been enriched due to their target-binding abilities. SELEX is relatively limited by the amount of enrichment that can be achieved in a single round. A less-reported screening method for aptamer fabrication that overcomes this limitation is affinity-based library enrichment that has been termed Particle Display.