Suppose the concentration of glutamine in an unknown sample needs to be measured. To do so, a series of standard solutions containing glutamine is prepared to create a calibration curve. A table summarizing a method for creating these solutions is shown below:
Here, a stock solution of glutamine is added in increasing increments with a high-accuracy instrument, such as a volumetric pipette, and diluted to the same volume in volumetric flasks. The resulting concentration is calculated using the formula for molar concentration. The result is 4 standard solutions with varying known concentrations plus a blank for instrument calibration.