Wöhler investigated more than twenty‐five chemical elements during his career. Hans Christian Ørsted was the first to separate the element aluminium in 1825, using a reduction of aluminium chloride with a potassium amalgam. Although Ørsted published his findings on the isolation of aluminium in the form of small particles, no other investigators successfully replicated his findings until 1936. Ørsted is now credited with discovering aluminium. Ørsteds findings on aluminium preparation were developed further by Wöhler, with Ørsteds permission. Wöhler modified Ørsted's methods, substituting potassium metal for potassium amalgam for the reduction of aluminium chloride. Using this improved method, Wöhler isolated aluminium powder in pure form on 22 October 1827. He showed that the aluminium powder could convert to solid balls of pure metallic aluminium in 1845. For this work, Wöhler is credited with the first isolation of aluminium in pure form.
In 1828 Wöhler was the first to isolate the element beryllium in pure metallic form (also independently isolated by Antoine Bussy). In the same year, he became the first to isolate the element yttrium in pure metallic form. He achieved these preparations by heating the anhydrous chlorides of beryllium and yttrium with potassium metal.
In 1850, Wöhler determined that what was believed until then to be metallic titanium was a mixture of titanium, carbon, and nitrogen, from which he derived the purest form isolated to that time. (Elemental titanium was later isolated in completely pure form in 1910 by Matthew A. Hunter.) He also developed a chemical synthesis of calcium carbide and silicon nitride.
Wöhler, working with French chemist Sainte Claire Deville, isolated the element boron in a crystalline form. He also isolated the element silicon in a crystalline form. Crystalline forms of these two elements were previously unknown. In 1856, working with Heinrich Buff, Wöhler prepared the inorganic compound silane (SiH). He prepared the first samples of boron nitride by melting together boric acid and potassium cyanide. He also developed a method for the preparation of calcium carbide.
Wöhler had an interest in the chemical composition of meteorites. He showed that some meteoric stones contain organic matter. He analyzed meteorites, and for many years wrote the digest on the literature of meteorites in the Jahresberichte über die Fortschritte der Chemie. Wöhler accumulated the best private collection of meteoric stones and irons that existed.