Lillie's trichrome is a combination of dyes used in histology.
It is similar to Massons trichrome stain, but it uses Biebrich scarlet for the plasma stain. It was initially published by Ralph D. Lillie in 1940. It is applied by submerging the fixated sample into the following three solutions: Weigerts iron hematoxylin working solution, Biebrich scarlet solution, and Fast Green FCF solution.
The resulting stains are black cell nuclei, brown cytoplasm, red muscle and myelinated fibers, blue collagen, and scarlet erythrocytes.