Some capillary techniques provide also a chamber like arrangement, however, there is no filter between the cells and the test substance. Quantitative results are gained by the multiwell type of this probe using 4-8-12-channel pipettes. Accuracy of the pipette and increased number of the parallel running samples is the great advantage of this test.
Counting of cells: positive responder cells are count from the lower chamber (long incubation time) or from the filter (short incubation time). For detection of cells general staining techniques (e.g. trypan blue) or special probes (e.g. mt-dehydrogenase detection with MTT assay) are used. Labelled (e.g. fluorochromes) cells are also used, in some assays cells get labelled during transmigration the filter.