The isomeric shift (also called isomer shift) is the shift on atomic spectral lines and gamma spectral lines, which occurs as a consequence of replacement of one nuclear isomer by another. It is usually called isomeric shift on atomic spectral lines and Mössbauer isomeric shift respectively. If the spectra also have hyperfine structure the shift refers to the center of gravity of the spectra. The isomeric shift provides important information about the nuclear structure and the physical, chemical or biological environment of atoms. More recently the effect has also been proposed as a tool in the search for the time variation of fundamental constants of nature.