Furthermore, one can assess whether the folding proceeds according to a two-state unfolding as described above. This can be done with differential scanning calorimetry by comparing the calorimetric enthalpy of denaturation i.e. the area under the peak,  to the van 't Hoff enthalpy described as follows:
at  the  can be described as:
When a two-state unfolding is observed the . The  is the height of the heat capacity peak.