In population ecology, a regulating factor, also known as a limiting factor, is something that keeps a population at equilibrium (neither increasing nor decreasing in size over time). Common limiting factor resources are environmental features that limit the growth, abundance, or distribution of an organism or a population of organisms in an ecosystem. The concept of limiting factors is based on Liebigs Law of the Minimum, which states that growth is controlled not by the total amount of resources available, but by the scarcest resource. In other words, a factor is limiting if a change in the factor produces increased growth, abundance, or distribution of an organism when other factors necessary to the organisms life do not. Limiting factors may be physical or biological.
Limiting factors are not limited to the condition of the species. Some factors may be increased or reduced based on circumstances. An example of a limiting factor is sunlight in the rain forest, where growth is limited to all plants on the forest floor unless more light becomes available. This decreases the number of potential factors that could influence a biological process, but only one is in effect at any one place and time. This recognition that there is always a single limiting factor is vital in ecology, and the concept has parallels in numerous other processes. The limiting factor also causes competition between individuals of a species population. For example, space is a limiting factor. Many predators and prey need a certain amount of space for survival: food, water, and other biological needs. If the population of a species is too high, they start competing for those needs. Thus the limiting factors hold down population in an area by causing some individuals to seek better prospects elsewhere and others to stay and starve. Some other limiting factors in biology include temperature and other weather related factors. Species can also be limited by the availability of macro- and micronutrients.  There has even been evidence of co-limitation in prairie ecosystems.  A study published in 2017 showed that sodium (a micronutrient) had no effect on its own, but when in combination with nitrogen and phosphorus (macronutrients), it did show positive effects, which is evidence of serial co-limitation.