To perform SCODA concentration of DNA molecules, the sample must be embedded in the separation media (gel) in locations where the electrophoretic field is of optimal intensity. This initial translocation of the sample into the optimal concentration position is referred to as "injection". The optimal position is determined by the gel geometry and location of the SCODA driving electrodes. Initially the sample is located in a buffer solution in the sample chamber, adjacent to the concentration gel. Injection is achieved by the application of a controlled DC electrophoretic field across the sample chamber which results in all charged particles being transferred into the concentration gel. To obtain a good stacking of the sample (i.e. tight DNA band) multiple methods can be employed. One example is to exploit the conductivity ratio between the sample chamber buffer and the concentration gel buffer. If the sample chamber buffer has a low conductivity and the concentration gel buffer has a high conductivity this results in a sharp drop off in electric field at the gel-buffer interface which promotes stacking.