Other modifications have also been used, such as the one described in Bagriantsev et al., using traditional wet transfer and a TGB buffering system, and others using semi-dry transfer or capillary transfer.
DD-AGE, a further variation of the method that uses fully denaturing conditions - including reducing agents such as dithiothreitol (DTT) and heat denaturation at 95°C - is suitable for the analysis of heat-stable inclusion bodies of polyglutamine proteins.