NMR spectroscopic research includes the following steps:
*Extraction of carbohydrate material (for natural glycans)
*Chemical removal of moieties masking regularity (for polymers)
*Separation and purification of carbohydrate material (for 2D NMR experiments, 10 mg or more is recommended)
*Sample preparation (usually in DO)
*Acquisition of 1D spectra 
*Planning, acquisition and processing of other NMR experiments (usually requires from 5 to 20 hours)
*Assignment and interpretation of spectra (see exemplary figure)
*If a structural problem could not be solved: chemical modification/degradation and NMR analysis of products
*Acquisition of spectra of the native (unmasked) compound and their interpretation based on modified structure
*Presentation of results