Upon reaction of a CDA with the target analyte, chromatography can be used to separate the resulting products. In general, chromatography can be used to separate chiral compounds to bypass difficult crystallizations and/or to collect all diastereomer pairs in solution. Chromatography also has many variations (e.g. HPLC, Gas Chromatography, flash chromatography) with a wide array of applicability to diverse categories of molecules. The ability for CDAs to separate chiral molecules is dependent on two major mechanisms of chromatography:
#Differential solvation in the mobile phase
#Differential adsorption to the stationary phase