It is well established that the macromolecular structure of nonviral gene delivery vectors alters their transfection efficacy and cytotoxicity. The cyclized structure has been proven to reduce cytotoxicity and increase circulation time for drug and gene delivery applications. The unique structure of cyclizing chains provides the single chain cyclized polymers a different method of interaction between the polymer and plasmid DNA, and results in a general trend of higher transfection capabilities than branched polymers. Moreover, due to the nature of the single chain structure, this cyclized polymer can “untie” to a linear chain under reducing conditions. Transfection profiles on astrocytes comparing 25 kDa-PEI, SuperFect® and Lipofectamine®2000 and cyclized polymer showed greater efficiency and cell viability whilst maintaining neural cell viability above 80% four days post transfections.