The primary sequence for FAM227B is isoform 1 with accession number: NP_689860.2. It is 508 amino acids long. There are 30 isoforms. The molecular weight is 59.9kD and the isoelectric point is predicted to be high, around 10. Compared to other proteins in humans, FAM227B has high abundance of Phenylalanine and Glycine and low abundance levels of Valine. The protein is predicted to be in the nuclear region of the cell. There is a bipartite nuclear localization signal at RKLERYGEFLKKYHKKK, and three other nuclearization signals at HKKK, KKKK, and PKKTKIK. There is also a vacuolar targeting motif at TLPI. An FWWh region, where h signifies hydrophobic, runs from amino acids 135-296 in Homo sapiens FAM227B isoform 1. The function of this region is still unknown.