In the two examples (on the right) the isoelectric point is shown by the green vertical line. In glycine the pK values are separated by nearly 7 units. Thus in the gas phase, the concentration of the neutral species, glycine (GlyH), is effectively 100% of the analytical glycine concentration. Glycine may exist as a zwitterion at the isoelectric point, but the equilibrium constant for the isomerization reaction in solution
is not known.
The other example, adenosine monophosphate is shown to illustrate the fact that a third species may, in principle, be involved. In fact the concentration of  is negligible at the isoelectric point in this case.
If the pI is greater than the pH, the molecule will have a positive charge.