Small differences in mass between stable isotopes of the same element can lead to a phenomenon called an "isotope effect," where heavier or lighter isotopes are preferentially incorporated into different natural materials depending on the materials chemical composition or physical state. Isotope effects are divided into two main groups: kinetic isotope effects and equilibrium isotope effects. A kinetic isotope effect occurs when a reaction is irreversible, meaning that the reaction only proceeds in the direction from reactants to products. Kinetic isotope effects cause isotopic fractionation—meaning that they affect the isotopic composition of reactant and product compounds—because the mass differences between stable isotopes can affect the rate of chemical reactions. It takes more energy to reach the transition state of a reaction if the compound has bonds with a heavier isotope, which causes the compound with heavier isotopes to react more slowly. Normal kinetic isotope effects cause the lighter isotope (or isotopes) to be preferentially included in a reactions product. The products are then said to be "depleted" in the heavy isotope relative to the reactant. Rarely, inverse kinetic isotope effects may occur, where the heavier isotope is preferentially included in a reaction's product.
Equilibrium isotope effects cause fractionation because it is more chemically favorable for heavy isotopes to take part in stronger bonds. An equilibrium isotope effect occurs when a reaction is at equilibrium, meaning that the reaction is able to occur in both directions simultaneously. When a reaction is at equilibrium, heavy isotopes will preferentially accumulate where they can form the strongest bonds. For example, when the water in a sealed, half-full bottle is in equilibrium with the vapor above it, the heavier isotopes H and O will accumulate in the liquid, where they form stronger bonds, while the lighter isotopes H and O will accumulate in the vapor. The liquid is then said to be "enriched" in the heavy isotope relative to the vapor.