In chemical kinetics, isosbestic points are used as reference points in the study of reaction rates, as the absorbance at those wavelengths remains constant throughout the whole reaction.
Isosbestic points are used in medicine in a laboratory technique called oximetry to determine hemoglobin concentration, regardless of its saturation. Oxyhaemoglobin and deoxyhaemoglobin have (not exclusively) isosbestic points at 586 nm and near 808 nm.
Isosbestic points are also used in clinical chemistry, as a quality assurance method, to verify the accuracy in the wavelength of a spectrophotometer. This is done by measuring the spectra of a standard solution at two different pH conditions (above and below the pK of the substance). The standards used include potassium dichromate (isosbestic points at 339 and 445 nm), bromothymol blue (325 and 498 nm) and congo red (541 nm). The wavelength of the isosbestic point determined does not depend on the concentration of the substance used, and so it becomes a very reliable reference.
One example of the use of isosbestic points in organic synthesis is seen in the photochemical A/D-corrin cycloisomerization ring closure reaction, which was the key step in the Eschenmoser / ETH Zürich vitamin B total synthesis. The isosbestic points provide proof for a direct conversion of the seco-corrin complex to the metal-free corrin ligand without intermediary or side products (within the detection limits of UV/VIS spectroscopy).