The general use and design of CDAs obey the following rules so that the CDA can effectively determine the stereochemistry of an analyte:
# The CDA must be enantiomerically pure, or (less satisfactorily) its enantiomeric purity must be accurately known.
#The reaction of the CDA with both enantiomers should go to completion under reaction conditions.  This acts to avoid enrichment or depletion of one enantiomer of the analyte by kinetic resolution.
# CDA must not racemize under derivatization or analysis conditions. Its attachment should be mild enough so that the substrate does not racemize either.  If analysis is completed by HPLC, the CDA must contain a chromophore to enhance detectability.  
# If analysis is completed by NMR, the CDA should have a functional group that gives a singlet in the resultant NMR spectrum, where the singlet must be remote from other peaks.