The dynamic behavior of Min proteins has been reconstituted in vitro using artificial lipid bilayers, with varying lipid composition and different confinement geometry as mimics for the cell membrane.  The first pattern to be reconstituted were spiraling waves of MinD chased by MinE, followed by the reconstitution of waves of all three proteins, MinD, MinE and MinC. Importantly, MinD and MinE can self-organize into a wide variety of patterns depending on the reaction conditions.
Additional study is required to elucidate the extent of temporal and spatial signaling permissible by this biological function. These in vitro systems offered unprecedented access to features such as residence times and molecular motility.