The following is a sample recipe for TBST:
*20 mM Tris
*150 mM NaCl
*0.1% Tween 20
Adjust pH with HCl to pH 7.4–7.6
The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water.