Strep Tamer is a technology which allows the reversible isolation and staining of antigen-specific T-cells. This technology combines a current T-cell isolation method with the Strep-Tag technology. In principle, the T-cells are separated by establishing a specific interaction between the T-cell of interest and a molecule, that is conjugated to a marker which enables the isolation. The reversibility of this interaction and the low temperatures at which it is performed allow for the isolation and characterization of functional T-cells. Because T-cells remain phenotypically and functionally indistinguishable from untreated cells, this method offers modern strategies in clinical and basic T-cell research.