Based on the sequencing approach used, the 5’ and 3’ adaptor sequences used to tag the cDNA library can be altered as needed. Previously, dual adapter-tagged cDNA libraries have been characterized using Illumina NGS. Low-cycle PCR can also be used to index universal adaptor cDNA libraries following the RT reaction. Alternatively, full-length adaptor sequences of choice can be included in the 5’ and 3’ adaptors used in the initial RT reaction.