Various bioinformatics tools can be used to analyze end-sequence profiling. Common ones include BreakDancer, PEMer, Variation Hunter, common LAW, GASV, and Spanner. ESP can be used to map structural variation at high-resolution in disease tissue. This technique is mainly used on tumor samples from different cancer types. Accurate identification of copy neutral chromosomal abnormalities is particularly important as translocation can lead to fusion proteins, chimeric proteins, or misregulated proteins that can be seen in tumors. This technique can also be used in evolution studies by identifying large structural variation between different populations. Similar methods are being developed for various applications. For example, a barcoded Illumina paired-end sequencing (BIPES) approach was used to assess microbial diversity by sequencing the 16S V6 tag.