Although m6A sites could be profiled at high resolution using UV-based methods, the stoichiometry of m6A sites - the methylation status or the ratio m6A+ to m6A- for each individual site within a type of RNA - is still unknown. SCARLET (2013) and m6A-LAIC-seq (2016) allows for the quantitation of stoichiometry at a specific locus and transcriptome-wide, respectively.
Bioinformatics methods used to analyze m6A peaks do not make any prior assumptions about the sequence motifs within which m6A sites are usually found, and take into consideration all possible motifs. Therefore, it is less likely to miss sites.