Velocyto is a package for the analysis of expression dynamics in single cell RNA seq data. In particular, it enables estimations of RNA velocities of single cells by distinguishing unspliced and spliced mRNAs in standard single-cell RNA sequencing protocols. It is the first paper proposed the concept of RNA velocity. velocyto predicted RNA velocity by solving the proposed differential equations for each gene. The authors envision future manifold learning algorithms that simultaneously fit a manifold and the kinetics on that manifold, on the basis of RNA velocity.