MDA can generate 1&ndash;2 µg of DNA from single cell with genome coverage of up to 99%. Products also have lower error rate and larger sizes compared to PCR based Taq amplification.
General work flow of MDA:
# Sample preparation: Samples are collected and diluted in the appropriate reaction buffer (Ca and Mg free). Cells are lysed with alkaline buffer.
# Condition: The MDA reaction with Ф29 polymerase is carried out at 30 °C. The reaction usually takes about 2.5&ndash;3 hours.
# End of reaction: Inactivate enzymes at 65 °C before collection of the amplified DNA products
# DNA products can be purified with commercial purification kit.