The second-generation of Glycoazodyes was first reported in 2008. These Glycoazodyes use an etherel linker. An ether group bonds the sugar and the dye to an n-alkane spacer, and the spacer bonds to the dye through another ether group. Like first-generation Glycoazodyes, second-generation Glycoazodyes use glucose, galactose or lactose as the sugar group.