N6,2'-O-dimethyladenosine, abundant in polyA+ mRNAs, occurs at the first nucleotide after the 5’ cap, when an additional methyl group is added to a 2ʹ-O-methyladenosine residue at the ‘capped’ 5ʹ end of mRNA.
Since m6Am can be recognized by anti-m6A antibodies at transcription start sites, the methods used for m6A profiling can be and were adapted for m6Am profiling, namely m6A-seq, and miCLIP (see m6A-seq and miCLIP descriptions above).