首先在GFP 基因中添加由4 个连续脯氨酸稀有密码子CCG 组成的阻遏区，结果显示该GFP 基因的表达量明显降低。
The expression of GFP in P. pastoris can be greatly reduced when a repressor region composed of four continuous proline rare codon CCG was added into the GFP gene.