<prompt> Theha teko ea lab ea phetoho ea baktheria.nan  <response>: Instruction in English: Construct a bacterial transformation lab experiment. . Response in English: In this bacterial transformation experiment, we will introduce a plasmid carrying an antibiotic resistance gene into E. coli bacteria, and observe the ability of the transformed bacteria to grow on a medium containing the antibiotic. Materials:- Petri dishes containing Luria-Bertani LB agar- LB broth- Ampicillin- Calcium chloride CaCl2- Plasmid DNA containing an ampicillin resistance gene e.g. pUC19- E. coli bacteria e.g. JM109 strain- Microcentrifuge tubes- Incubator- Heat block- Pipettes Method:1. Begin by preparing the LB agar plates for the experiment. Mix ampicillin into the LB agar to a final concentration of 100 μgmL. Pour the LBampicillin agar into Petri dishes, and allow it to solidify.2. Thaw the competent E. coli cells, and place them on ice.3. Aliquot 50 μL of the competent cells into a microcentrifuge tube.4. Add 10 ng of the plasmid DNA to the tube containing the competent cells.5. Gently tap the tube to mix the cells and DNA, and then incubate the tube on ice for 30 minutes.6. After incubation, heat shock the cells by placing the tube in a heat block at 42C for 45 seconds.7. Immediately return the tube to the ice, and incubate for another 2 minutes.8. Add 250 μL of LB broth to the tube, and gently mix the contents.9. Incubate the tube at 37C for 1 hour, with gentle shaking, to allow the cells to recover and express the ampicillin resistance gene.10. Spread 100 μL of the transformation mixture onto the prepared LBampicillin agar plates.11. Incubate the plates at 37C for 16-18 hours. Results:After incubation, check the plates for growth. If the transformation was successful, there should be visible colonies of bacteria growing on the LBampicillin agar plates. These colonies represent individual bacterial cells that have taken up the plasmid DNA, and are expressing the ampicillin resistance gene, allowing them to grow in the presence of the antibiotic.If there is no growth on the plates, this indicates that the transformation was not successful . Response in Sesotho: Tekong ena ea phetoho ea baktheria, re tla hlahisa plasmid e jereng phatsa ea lefutso ea lithibela-mafu ka hara baktheria ea E. coli, me re hlokomele bokhoni ba baktheria e fetohileng ea ho hōla ka mokhoa o nang le sebolaya-mafu. Lisebelisoa: - Lijana tsa Petri tse nang le Luria-Bertani LB agar - LB broth - Ampicillin - Calcium chloride CaCl2 - Plasmid DNA e nang le mofuta oa ampicillin resistance mohlala pUC19 - E. coli bacteria mohlala mofuta oa JM109 - Microcentrifuge li-tubes - Incubator - Heat block - Pipettes Mokhoa: 1. Qala ka ho lokisa lipoleiti tsa agar tsa LB bakeng sa teko. Kopanya ampicillin ka hara LB agar ho ea ho 100 μgmL ea ho qetela. Tšela LBampicillin agar ka hara lijana tsa Petri, me u e lumelle hore e tiee. 2. Qhibiliha lisele tse nang le bokhoni ba E. coli, me u li behe holima leqhoa. 3. Aliquot 50 μL ea lisele tse nang le bokhoni ka hara tube ea microcentrifuge. 4. Kenya 10 ng ea plasmid DNA ho tube e nang le lisele tse nang le bokhoni. 5. Tlanya tube ka bonolo ho kopanya lisele le DNA, ebe u e kenya ka hara leqhoa ka metsotso e 30. 6. Ka mora ho kenngoa, mocheso o tšosa lisele ka ho beha tube ka hara mocheso oa mocheso oa 42 C ka metsotsoana e 45. 7. Hang-hang khutlisetsa tube ho leqhoa, me u emise metsotso e meng ea 2. 8. Kenya 250 μL ea moro oa LB ka hara tube, me ka bonolo kopanya likahare. 9. Eketsa tube ka 37 C bakeng sa hora e le ngoe, ka ho sisinyeha ha bonolo, ho lumella lisele hore li hlaphoheloe le ho hlahisa mofuta oa ho hanyetsa ampicillin. 10. Hasa 100 μL ea motsoako oa phetoho holima lipoleiti tsa agar tsa LBampicillin tse lokisitsoeng. 11. Koahela lipoleiti ka 37 C bakeng sa lihora tse 16-18. Liphetho: Kamora ho qhoqhoa, hlahloba lipoleiti bakeng sa kholo. Haeba phetoho e atlehile, ho lokela ho ba le likolone tse bonahalang tsa libaktheria tse ntseng li hola holima lipoleiti tsa agar LBampicillin. Likolone tsena li emela lisele tsa baktheria tse nkileng plasmid DNA, me li hlahisa mofuta oa ampicillin oa ho hanyetsa, o li lumellang ho hola boteng ba lithibela-mafu. Haeba ho se na khōlo holima lipoleiti, sena se bontša hore phetoho ha ea ka ea atleha 