12 sections contains 56 chapters. As usual, Methods in Enzymology has a detailed subject INDEX and author INDEX at the end.Section I: General methodology; anesthesia etc.Section II: Germ cells and embryos; oocytes culture, acrosome reaction etc.Section III: Fertilization;Section IV: Gene expression: mRNA; quantitative analysis of mRNASection V: Gene expression: Reporter genes; luciferase, lacZ etc.Section VI: Gene expression: protein; 2-D gel, immunohistochem etc.Section VII: Gene expression: methylation; analysis of methylation by PCR etc.Section VIII: Gene identification; cDNA library construction, cloning etc.Section IX: Nuclear transplantation; transplantation of nuclei to oocytes etc.Section X: Transgenic animals: pronuclear injection;Section XI: Transgenic animals: Embryonic stem cells and gene targetingSection XII: Lineage analysis